Molecular and genome characterization of colistin-resistant Escherichia coli isolates from wild sea lions (Zalophus californianus)
Using molecular and whole-genome sequencing tools, we investigated colistin-resistant Escherichia coli isolates from wild sea lions. Two unrelated E. coli colistin-resistant isolates, ST8259 and ST4218, were identified, both belonging to the B2 phylogroup and different serotypes. Polymorphisms in Pm...
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| Veröffentlicht in: | Brazilian journal of microbiology 2020-12, Vol.51 (4), p.2009-2014 |
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| container_title | Brazilian journal of microbiology |
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| creator | Hernández-Castro, Rigoberto Rodríguez-Santiago, Jonathan Téllez-Sosa, Juan Bravo-Romero, Sugey Silva-Sánchez, Jesús Sánchez-Pérez, Alejandro Avalos-Téllez, Rosalia Martínez-Chavarría, Luary Carolina Xicohtencatl-Cortes, Juan Garza-Ramos, Ulises |
| description | Using molecular and whole-genome sequencing tools, we investigated colistin-resistant
Escherichia coli
isolates from wild sea lions. Two unrelated
E. coli
colistin-resistant isolates, ST8259 and ST4218, were identified, both belonging to the B2 phylogroup and different serotypes. Polymorphisms in PmrA, PmrB, and PhoQ proteins were identified, and the role of PmrB and PhoQ in contributing to colistin resistance was determined by complementation assays. However, the mutations characterized in the present study are not involved in colistin resistance, which have been described in
E. coli
isolates from clinical settings. Therefore, the acquired mutations in
pmrB
and
phoQ
genes in resistance to colistin in bacteria related to marine environment animals are different. This work contributes to the surveillance and characterization of colistin resistance in
Escherichia coli
obtained from animals from aquatic environments. |
| doi_str_mv | 10.1007/s42770-020-00371-5 |
| format | Article |
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Escherichia coli
isolates from wild sea lions. Two unrelated
E. coli
colistin-resistant isolates, ST8259 and ST4218, were identified, both belonging to the B2 phylogroup and different serotypes. Polymorphisms in PmrA, PmrB, and PhoQ proteins were identified, and the role of PmrB and PhoQ in contributing to colistin resistance was determined by complementation assays. However, the mutations characterized in the present study are not involved in colistin resistance, which have been described in
E. coli
isolates from clinical settings. Therefore, the acquired mutations in
pmrB
and
phoQ
genes in resistance to colistin in bacteria related to marine environment animals are different. This work contributes to the surveillance and characterization of colistin resistance in
Escherichia coli
obtained from animals from aquatic environments.</description><identifier>ISSN: 1517-8382</identifier><identifier>ISSN: 1678-4405</identifier><identifier>EISSN: 1678-4405</identifier><identifier>DOI: 10.1007/s42770-020-00371-5</identifier><identifier>PMID: 32897511</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Animals ; Animals, Wild - microbiology ; Anti-Bacterial Agents - pharmacology ; Biomedical and Life Sciences ; Colistin - pharmacology ; Drug Resistance, Multiple, Bacterial - genetics ; Environmental Microbiology - Short Communication ; Escherichia coli - classification ; Escherichia coli - drug effects ; Escherichia coli Proteins - genetics ; Food Microbiology ; Genome, Bacterial ; Life Sciences ; Medical Microbiology ; Microbial Ecology ; Microbial Genetics and Genomics ; Microbial Sensitivity Tests ; Microbiology ; Mycology ; Sea Lions - microbiology ; Serogroup ; Whole Genome Sequencing</subject><ispartof>Brazilian journal of microbiology, 2020-12, Vol.51 (4), p.2009-2014</ispartof><rights>Sociedade Brasileira de Microbiologia 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3615-1b3d9f11c27a7e7063104b72d17dfbedaa004b95cc916c20b894c8552e9bf203</citedby><cites>FETCH-LOGICAL-c3615-1b3d9f11c27a7e7063104b72d17dfbedaa004b95cc916c20b894c8552e9bf203</cites><orcidid>0000-0002-5013-3050</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7688835/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7688835/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,41488,42557,51319,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32897511$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hernández-Castro, Rigoberto</creatorcontrib><creatorcontrib>Rodríguez-Santiago, Jonathan</creatorcontrib><creatorcontrib>Téllez-Sosa, Juan</creatorcontrib><creatorcontrib>Bravo-Romero, Sugey</creatorcontrib><creatorcontrib>Silva-Sánchez, Jesús</creatorcontrib><creatorcontrib>Sánchez-Pérez, Alejandro</creatorcontrib><creatorcontrib>Avalos-Téllez, Rosalia</creatorcontrib><creatorcontrib>Martínez-Chavarría, Luary Carolina</creatorcontrib><creatorcontrib>Xicohtencatl-Cortes, Juan</creatorcontrib><creatorcontrib>Garza-Ramos, Ulises</creatorcontrib><title>Molecular and genome characterization of colistin-resistant Escherichia coli isolates from wild sea lions (Zalophus californianus)</title><title>Brazilian journal of microbiology</title><addtitle>Braz J Microbiol</addtitle><addtitle>Braz J Microbiol</addtitle><description>Using molecular and whole-genome sequencing tools, we investigated colistin-resistant
Escherichia coli
isolates from wild sea lions. Two unrelated
E. coli
colistin-resistant isolates, ST8259 and ST4218, were identified, both belonging to the B2 phylogroup and different serotypes. Polymorphisms in PmrA, PmrB, and PhoQ proteins were identified, and the role of PmrB and PhoQ in contributing to colistin resistance was determined by complementation assays. However, the mutations characterized in the present study are not involved in colistin resistance, which have been described in
E. coli
isolates from clinical settings. Therefore, the acquired mutations in
pmrB
and
phoQ
genes in resistance to colistin in bacteria related to marine environment animals are different. This work contributes to the surveillance and characterization of colistin resistance in
Escherichia coli
obtained from animals from aquatic environments.</description><subject>Animals</subject><subject>Animals, Wild - microbiology</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Biomedical and Life Sciences</subject><subject>Colistin - pharmacology</subject><subject>Drug Resistance, Multiple, Bacterial - genetics</subject><subject>Environmental Microbiology - Short Communication</subject><subject>Escherichia coli - classification</subject><subject>Escherichia coli - drug effects</subject><subject>Escherichia coli Proteins - genetics</subject><subject>Food Microbiology</subject><subject>Genome, Bacterial</subject><subject>Life Sciences</subject><subject>Medical Microbiology</subject><subject>Microbial Ecology</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbial Sensitivity Tests</subject><subject>Microbiology</subject><subject>Mycology</subject><subject>Sea Lions - microbiology</subject><subject>Serogroup</subject><subject>Whole Genome Sequencing</subject><issn>1517-8382</issn><issn>1678-4405</issn><issn>1678-4405</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUtv1TAQhS0EoqXwB1ggL8si4EccOxskVJWHVMSmKzbWxHFuXDn2xZOAYMkvx7e3VLBhYXmsc-Ybaw4hzzl7xRnTr7EVWrOGiXqY1LxRD8gp77Rp2paph7VWXDdGGnFCniDeMCYUa8VjciKF6bXi_JT8-pSjd1uEQiGNdOdTXjx1MxRwqy_hJ6whJ5on6nIMuIbUFI-1gLTSS3Rz9bg5wK1MA-YIq0c6lbzQ7yGOFD3QWBFIz79AzPt5Q-oghimXFCBt-PIpeTRBRP_s7j4j1-8ury8-NFef33-8eHvVONlx1fBBjv3EuRMatNesk5y1gxYj1-M0-BGA1XevnOt55wQbTN86o5Tw_TAJJs_ImyN2vw2LH51Pa4Fo9yUsUH7YDMH-q6Qw213-ZnVnjJGqAs7vACV_3TyudgnofIyQfN7Qirr1nrWyPVjF0epKRix-uh_DmT1kZ4_Z2Zqdvc3OHppe_P3B-5Y_YVWDPBqwSmnni73JW0l1Z__D_gZh8ain</recordid><startdate>20201201</startdate><enddate>20201201</enddate><creator>Hernández-Castro, Rigoberto</creator><creator>Rodríguez-Santiago, Jonathan</creator><creator>Téllez-Sosa, Juan</creator><creator>Bravo-Romero, Sugey</creator><creator>Silva-Sánchez, Jesús</creator><creator>Sánchez-Pérez, Alejandro</creator><creator>Avalos-Téllez, Rosalia</creator><creator>Martínez-Chavarría, Luary Carolina</creator><creator>Xicohtencatl-Cortes, Juan</creator><creator>Garza-Ramos, Ulises</creator><general>Springer International Publishing</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-5013-3050</orcidid></search><sort><creationdate>20201201</creationdate><title>Molecular and genome characterization of colistin-resistant Escherichia coli isolates from wild sea lions (Zalophus californianus)</title><author>Hernández-Castro, Rigoberto ; Rodríguez-Santiago, Jonathan ; Téllez-Sosa, Juan ; Bravo-Romero, Sugey ; Silva-Sánchez, Jesús ; Sánchez-Pérez, Alejandro ; Avalos-Téllez, Rosalia ; Martínez-Chavarría, Luary Carolina ; Xicohtencatl-Cortes, Juan ; Garza-Ramos, Ulises</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3615-1b3d9f11c27a7e7063104b72d17dfbedaa004b95cc916c20b894c8552e9bf203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Animals, Wild - microbiology</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Biomedical and Life Sciences</topic><topic>Colistin - pharmacology</topic><topic>Drug Resistance, Multiple, Bacterial - genetics</topic><topic>Environmental Microbiology - Short Communication</topic><topic>Escherichia coli - classification</topic><topic>Escherichia coli - drug effects</topic><topic>Escherichia coli Proteins - genetics</topic><topic>Food Microbiology</topic><topic>Genome, Bacterial</topic><topic>Life Sciences</topic><topic>Medical Microbiology</topic><topic>Microbial Ecology</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbial Sensitivity Tests</topic><topic>Microbiology</topic><topic>Mycology</topic><topic>Sea Lions - microbiology</topic><topic>Serogroup</topic><topic>Whole Genome Sequencing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hernández-Castro, Rigoberto</creatorcontrib><creatorcontrib>Rodríguez-Santiago, Jonathan</creatorcontrib><creatorcontrib>Téllez-Sosa, Juan</creatorcontrib><creatorcontrib>Bravo-Romero, Sugey</creatorcontrib><creatorcontrib>Silva-Sánchez, Jesús</creatorcontrib><creatorcontrib>Sánchez-Pérez, Alejandro</creatorcontrib><creatorcontrib>Avalos-Téllez, Rosalia</creatorcontrib><creatorcontrib>Martínez-Chavarría, Luary Carolina</creatorcontrib><creatorcontrib>Xicohtencatl-Cortes, Juan</creatorcontrib><creatorcontrib>Garza-Ramos, Ulises</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Brazilian journal of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hernández-Castro, Rigoberto</au><au>Rodríguez-Santiago, Jonathan</au><au>Téllez-Sosa, Juan</au><au>Bravo-Romero, Sugey</au><au>Silva-Sánchez, Jesús</au><au>Sánchez-Pérez, Alejandro</au><au>Avalos-Téllez, Rosalia</au><au>Martínez-Chavarría, Luary Carolina</au><au>Xicohtencatl-Cortes, Juan</au><au>Garza-Ramos, Ulises</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular and genome characterization of colistin-resistant Escherichia coli isolates from wild sea lions (Zalophus californianus)</atitle><jtitle>Brazilian journal of microbiology</jtitle><stitle>Braz J Microbiol</stitle><addtitle>Braz J Microbiol</addtitle><date>2020-12-01</date><risdate>2020</risdate><volume>51</volume><issue>4</issue><spage>2009</spage><epage>2014</epage><pages>2009-2014</pages><issn>1517-8382</issn><issn>1678-4405</issn><eissn>1678-4405</eissn><abstract>Using molecular and whole-genome sequencing tools, we investigated colistin-resistant
Escherichia coli
isolates from wild sea lions. Two unrelated
E. coli
colistin-resistant isolates, ST8259 and ST4218, were identified, both belonging to the B2 phylogroup and different serotypes. Polymorphisms in PmrA, PmrB, and PhoQ proteins were identified, and the role of PmrB and PhoQ in contributing to colistin resistance was determined by complementation assays. However, the mutations characterized in the present study are not involved in colistin resistance, which have been described in
E. coli
isolates from clinical settings. Therefore, the acquired mutations in
pmrB
and
phoQ
genes in resistance to colistin in bacteria related to marine environment animals are different. This work contributes to the surveillance and characterization of colistin resistance in
Escherichia coli
obtained from animals from aquatic environments.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>32897511</pmid><doi>10.1007/s42770-020-00371-5</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-5013-3050</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1517-8382 |
| ispartof | Brazilian journal of microbiology, 2020-12, Vol.51 (4), p.2009-2014 |
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| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7688835 |
| source | MEDLINE; PubMed Central; Alma/SFX Local Collection; SpringerLink Journals - AutoHoldings |
| subjects | Animals Animals, Wild - microbiology Anti-Bacterial Agents - pharmacology Biomedical and Life Sciences Colistin - pharmacology Drug Resistance, Multiple, Bacterial - genetics Environmental Microbiology - Short Communication Escherichia coli - classification Escherichia coli - drug effects Escherichia coli Proteins - genetics Food Microbiology Genome, Bacterial Life Sciences Medical Microbiology Microbial Ecology Microbial Genetics and Genomics Microbial Sensitivity Tests Microbiology Mycology Sea Lions - microbiology Serogroup Whole Genome Sequencing |
| title | Molecular and genome characterization of colistin-resistant Escherichia coli isolates from wild sea lions (Zalophus californianus) |
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