Influence of FRET and fluorescent protein maturation on the quantification of binding affinity with dual-channel fluorescence cross-correlation spectroscopy

Protein-protein interactions at the plasma membrane mediate transmembrane signaling. Dual-channel fluorescence cross-correlation spectroscopy (dc-FCCS) is a method with which these interactions can be quantified in a cellular context. However, factors such as incomplete maturation of fluorescent pro...

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Veröffentlicht in:	Biomedical optics express 2020-11, Vol.11 (11), p.6137-6153
Hauptverfasser:	Sreenivasan, Varun K A, Graus, Matthew S, Pillai, Rashmi R, Yang, Zhengmin, Goyette, Jesse, Gaus, Katharina
Format:	Artikel
Sprache:	eng
Online-Zugang:	Volltext
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