3D Printed Multiplexed Competitive Migration Assays with Spatially Programmable Release Sources
Here, a 3D printed multiplexed competitive migration assay is reported for characterizing a chemotactic response in the presence of multiple spatially distributed chemoattractants. The utility of the assay is demonstrated by examining the chemotactic response of human glioblastoma cells to spatially...
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| Veröffentlicht in: | Advanced biosystems 2020-01, Vol.4 (1), p.e1900225-n/a |
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| creator | Haring, Alexander P. Thompson, Emily G. Hernandez, Raymundo D. Laheri, Sahil Harrigan, Megan E. Lear, Taylor Sontheimer, Harald Johnson, Blake N. |
| description | Here, a 3D printed multiplexed competitive migration assay is reported for characterizing a chemotactic response in the presence of multiple spatially distributed chemoattractants. The utility of the assay is demonstrated by examining the chemotactic response of human glioblastoma cells to spatially opposing chemotactic gradients of epidermal growth factor (EGF) and bradykinin (BK). Competitive migration assays involving spatially opposing gradients of EGF and BK that are optimized in the absence of the second chemoattractant show that 46% more glioblastoma cells migrate toward EGF sources. The migration velocities of human glioblastoma cells toward EGF and BK sources are reduced by 7.6 ± 2.2% and 11.6 ± 6.3% relative to those found in the absence of the spatially opposing chemoattractant. This work provides new insight to the chemotactic response associated with glioblastoma‐vasculature interactions and a versatile, user‐friendly platform for characterizing the chemotactic response of cells in the presence of multiple spatially distributed chemoattractants.
A 3D printed migration assay for the analysis of a chemotactic response in the presence of spatially distributed sources of chemoattractants is presented. The assay enables multiplexed studies with on‐plate controls. The device has broad applications ranging from the analysis of competitive chemotactic responses associated with diseases and development of 3D printed constructs. |
| doi_str_mv | 10.1002/adbi.201900225 |
| format | Article |
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A 3D printed migration assay for the analysis of a chemotactic response in the presence of spatially distributed sources of chemoattractants is presented. The assay enables multiplexed studies with on‐plate controls. The device has broad applications ranging from the analysis of competitive chemotactic responses associated with diseases and development of 3D printed constructs.</description><identifier>ISSN: 2366-7478</identifier><identifier>EISSN: 2366-7478</identifier><identifier>DOI: 10.1002/adbi.201900225</identifier><identifier>PMID: 32293127</identifier><language>eng</language><publisher>Germany</publisher><subject>Bradykinin - pharmacology ; Cell Line, Tumor ; Cell Migration Assays - instrumentation ; Cell Migration Assays - methods ; Chemotactic Factors - pharmacology ; Chemotaxis - drug effects ; Epidermal Growth Factor - pharmacology ; Equipment Design ; Glioblastoma ; gradients ; Humans ; microextrusion 3D printing ; Microfluidic Analytical Techniques - instrumentation ; microphysiological neural systems ; neural system‐on‐a‐chip ; organ‐on‐a‐chip ; Printing, Three-Dimensional</subject><ispartof>Advanced biosystems, 2020-01, Vol.4 (1), p.e1900225-n/a</ispartof><rights>2019 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4405-111d3b9e308f78220aa240de8946a1507b26c422d5a91fd8df1961b713c1a1643</citedby><cites>FETCH-LOGICAL-c4405-111d3b9e308f78220aa240de8946a1507b26c422d5a91fd8df1961b713c1a1643</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fadbi.201900225$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fadbi.201900225$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,776,780,881,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32293127$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Haring, Alexander P.</creatorcontrib><creatorcontrib>Thompson, Emily G.</creatorcontrib><creatorcontrib>Hernandez, Raymundo D.</creatorcontrib><creatorcontrib>Laheri, Sahil</creatorcontrib><creatorcontrib>Harrigan, Megan E.</creatorcontrib><creatorcontrib>Lear, Taylor</creatorcontrib><creatorcontrib>Sontheimer, Harald</creatorcontrib><creatorcontrib>Johnson, Blake N.</creatorcontrib><title>3D Printed Multiplexed Competitive Migration Assays with Spatially Programmable Release Sources</title><title>Advanced biosystems</title><addtitle>Adv Biosyst</addtitle><description>Here, a 3D printed multiplexed competitive migration assay is reported for characterizing a chemotactic response in the presence of multiple spatially distributed chemoattractants. The utility of the assay is demonstrated by examining the chemotactic response of human glioblastoma cells to spatially opposing chemotactic gradients of epidermal growth factor (EGF) and bradykinin (BK). Competitive migration assays involving spatially opposing gradients of EGF and BK that are optimized in the absence of the second chemoattractant show that 46% more glioblastoma cells migrate toward EGF sources. The migration velocities of human glioblastoma cells toward EGF and BK sources are reduced by 7.6 ± 2.2% and 11.6 ± 6.3% relative to those found in the absence of the spatially opposing chemoattractant. This work provides new insight to the chemotactic response associated with glioblastoma‐vasculature interactions and a versatile, user‐friendly platform for characterizing the chemotactic response of cells in the presence of multiple spatially distributed chemoattractants.
A 3D printed migration assay for the analysis of a chemotactic response in the presence of spatially distributed sources of chemoattractants is presented. The assay enables multiplexed studies with on‐plate controls. The device has broad applications ranging from the analysis of competitive chemotactic responses associated with diseases and development of 3D printed constructs.</description><subject>Bradykinin - pharmacology</subject><subject>Cell Line, Tumor</subject><subject>Cell Migration Assays - instrumentation</subject><subject>Cell Migration Assays - methods</subject><subject>Chemotactic Factors - pharmacology</subject><subject>Chemotaxis - drug effects</subject><subject>Epidermal Growth Factor - pharmacology</subject><subject>Equipment Design</subject><subject>Glioblastoma</subject><subject>gradients</subject><subject>Humans</subject><subject>microextrusion 3D printing</subject><subject>Microfluidic Analytical Techniques - instrumentation</subject><subject>microphysiological neural systems</subject><subject>neural system‐on‐a‐chip</subject><subject>organ‐on‐a‐chip</subject><subject>Printing, Three-Dimensional</subject><issn>2366-7478</issn><issn>2366-7478</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkFtPwjAYhhujEYLcemn2B4b92q1rb0wQPJBANKLXTbd1UNMdsg5w_94RFPXKq-_0vs-XvAhdAh4BxuRapbEZEQyiG0h4gvqEMuZHQcRPf_U9NHTuHWMMnFEeinPUo4QICiTqI0mn3nNtikan3mJjG1NZ_dH1kzKvdGMas9Xewqxq1Ziy8MbOqdZ5O9OsvWXV7ZS1becvO0Geq9hq70VbrZz2luWmTrS7QGeZsk4Pv-oAvd3fvU4e_fnTw2wynvtJEODQB4CUxkJTzLOIE4KVIgFONRcBUxDiKCYsCQhJQyUgS3magWAQR0ATUMACOkA3B261iXOdJrpoamVlVZtc1a0slZF_L4VZy1W5lRHjEQ_DDjA6AJK6dK7W2dELWO7Tlvu05THtznD1--NR_p1tJxAHwc5Y3f6Dk-Pp7ewH_gnS041Z</recordid><startdate>202001</startdate><enddate>202001</enddate><creator>Haring, Alexander P.</creator><creator>Thompson, Emily G.</creator><creator>Hernandez, Raymundo D.</creator><creator>Laheri, Sahil</creator><creator>Harrigan, Megan E.</creator><creator>Lear, Taylor</creator><creator>Sontheimer, Harald</creator><creator>Johnson, Blake N.</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>202001</creationdate><title>3D Printed Multiplexed Competitive Migration Assays with Spatially Programmable Release Sources</title><author>Haring, Alexander P. ; Thompson, Emily G. ; Hernandez, Raymundo D. ; Laheri, Sahil ; Harrigan, Megan E. ; Lear, Taylor ; Sontheimer, Harald ; Johnson, Blake N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4405-111d3b9e308f78220aa240de8946a1507b26c422d5a91fd8df1961b713c1a1643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Bradykinin - pharmacology</topic><topic>Cell Line, Tumor</topic><topic>Cell Migration Assays - instrumentation</topic><topic>Cell Migration Assays - methods</topic><topic>Chemotactic Factors - pharmacology</topic><topic>Chemotaxis - drug effects</topic><topic>Epidermal Growth Factor - pharmacology</topic><topic>Equipment Design</topic><topic>Glioblastoma</topic><topic>gradients</topic><topic>Humans</topic><topic>microextrusion 3D printing</topic><topic>Microfluidic Analytical Techniques - instrumentation</topic><topic>microphysiological neural systems</topic><topic>neural system‐on‐a‐chip</topic><topic>organ‐on‐a‐chip</topic><topic>Printing, Three-Dimensional</topic><toplevel>online_resources</toplevel><creatorcontrib>Haring, Alexander P.</creatorcontrib><creatorcontrib>Thompson, Emily G.</creatorcontrib><creatorcontrib>Hernandez, Raymundo D.</creatorcontrib><creatorcontrib>Laheri, Sahil</creatorcontrib><creatorcontrib>Harrigan, Megan E.</creatorcontrib><creatorcontrib>Lear, Taylor</creatorcontrib><creatorcontrib>Sontheimer, Harald</creatorcontrib><creatorcontrib>Johnson, Blake N.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Advanced biosystems</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Haring, Alexander P.</au><au>Thompson, Emily G.</au><au>Hernandez, Raymundo D.</au><au>Laheri, Sahil</au><au>Harrigan, Megan E.</au><au>Lear, Taylor</au><au>Sontheimer, Harald</au><au>Johnson, Blake N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>3D Printed Multiplexed Competitive Migration Assays with Spatially Programmable Release Sources</atitle><jtitle>Advanced biosystems</jtitle><addtitle>Adv Biosyst</addtitle><date>2020-01</date><risdate>2020</risdate><volume>4</volume><issue>1</issue><spage>e1900225</spage><epage>n/a</epage><pages>e1900225-n/a</pages><issn>2366-7478</issn><eissn>2366-7478</eissn><abstract>Here, a 3D printed multiplexed competitive migration assay is reported for characterizing a chemotactic response in the presence of multiple spatially distributed chemoattractants. The utility of the assay is demonstrated by examining the chemotactic response of human glioblastoma cells to spatially opposing chemotactic gradients of epidermal growth factor (EGF) and bradykinin (BK). Competitive migration assays involving spatially opposing gradients of EGF and BK that are optimized in the absence of the second chemoattractant show that 46% more glioblastoma cells migrate toward EGF sources. The migration velocities of human glioblastoma cells toward EGF and BK sources are reduced by 7.6 ± 2.2% and 11.6 ± 6.3% relative to those found in the absence of the spatially opposing chemoattractant. This work provides new insight to the chemotactic response associated with glioblastoma‐vasculature interactions and a versatile, user‐friendly platform for characterizing the chemotactic response of cells in the presence of multiple spatially distributed chemoattractants.
A 3D printed migration assay for the analysis of a chemotactic response in the presence of spatially distributed sources of chemoattractants is presented. The assay enables multiplexed studies with on‐plate controls. The device has broad applications ranging from the analysis of competitive chemotactic responses associated with diseases and development of 3D printed constructs.</abstract><cop>Germany</cop><pmid>32293127</pmid><doi>10.1002/adbi.201900225</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Bradykinin - pharmacology Cell Line, Tumor Cell Migration Assays - instrumentation Cell Migration Assays - methods Chemotactic Factors - pharmacology Chemotaxis - drug effects Epidermal Growth Factor - pharmacology Equipment Design Glioblastoma gradients Humans microextrusion 3D printing Microfluidic Analytical Techniques - instrumentation microphysiological neural systems neural system‐on‐a‐chip organ‐on‐a‐chip Printing, Three-Dimensional |
| title | 3D Printed Multiplexed Competitive Migration Assays with Spatially Programmable Release Sources |
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