Bone marrow mesenchymal stem cell-derived exosomes promote rotator cuff tendon-bone healing by promoting angiogenesis and regulating M1 macrophages in rats
Rotator cuff tears (RCTs) often require reconstructive surgery. Tendon-bone healing is critical for the outcome of rotator cuff reconstruction, but the process of tendon-bone healing is complex and difficult. Mesenchymal stem cells (MSCs) are considered to be an effective method to promote tendon-bo...
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| Veröffentlicht in: | Stem cell research & therapy 2020-11, Vol.11 (1), p.496-496, Article 496 |
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| description | Rotator cuff tears (RCTs) often require reconstructive surgery. Tendon-bone healing is critical for the outcome of rotator cuff reconstruction, but the process of tendon-bone healing is complex and difficult. Mesenchymal stem cells (MSCs) are considered to be an effective method to promote tendon-bone healing. MSCs have strong paracrine, anti-inflammatory, immunoregulatory, and angiogenic potential. Recent studies have shown that MSCs achieve many regulatory functions through exosomes. The purpose of this study was to explore the role of bone marrow mesenchymal stem cell-derived exosomes (BMSC-Exos) in tendon-bone healing.
Our study found that BMSC-Exos promote the proliferation, migration, and angiogenic tube formation of human umbilical vein endothelial cells (HUVECs). The mechanism by which BMSC-Exos achieve this may be through the regulation of the angiogenic signaling pathway. In addition, BMSC-Exos can inhibit the polarization of M1 macrophages and inhibit the secretion of proinflammatory factors by M1 macrophages. After rotator cuff reconstruction in rats, BMSC-Exos were injected into the tail vein to analyze their effect on the rotator cuff tendon-bone interface healing.
It was confirmed that BMSC-Exos increased the breaking load and stiffness of the rotator cuff after reconstruction in rats, induced angiogenesis around the rotator cuff endpoint, and promoted growth of the tendon-bone interface.
BMSC-Exos promote tendon-bone healing after rotator cuff reconstruction in rats by promoting angiogenesis and inhibiting inflammation. |
| doi_str_mv | 10.1186/s13287-020-02005-x |
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Our study found that BMSC-Exos promote the proliferation, migration, and angiogenic tube formation of human umbilical vein endothelial cells (HUVECs). The mechanism by which BMSC-Exos achieve this may be through the regulation of the angiogenic signaling pathway. In addition, BMSC-Exos can inhibit the polarization of M1 macrophages and inhibit the secretion of proinflammatory factors by M1 macrophages. After rotator cuff reconstruction in rats, BMSC-Exos were injected into the tail vein to analyze their effect on the rotator cuff tendon-bone interface healing.
It was confirmed that BMSC-Exos increased the breaking load and stiffness of the rotator cuff after reconstruction in rats, induced angiogenesis around the rotator cuff endpoint, and promoted growth of the tendon-bone interface.
BMSC-Exos promote tendon-bone healing after rotator cuff reconstruction in rats by promoting angiogenesis and inhibiting inflammation.</description><identifier>ISSN: 1757-6512</identifier><identifier>EISSN: 1757-6512</identifier><identifier>DOI: 10.1186/s13287-020-02005-x</identifier><identifier>PMID: 33239091</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Angiogenesis ; Antibiotics ; Antibodies ; Bone growth ; Bone healing ; Bone marrow ; Bone surgery ; Cartilage ; Cell proliferation ; Collagen ; Endothelial cells ; Exosomes ; Growth factors ; Immunoregulation ; Inflammation ; Injuries ; Leukocyte migration ; Macrophages ; Medical research ; Mesenchymal stem cells ; Paracrine signalling ; Proteins ; Reconstructive surgery ; Rotator cuff ; Signal transduction ; Stem cell transplantation ; Stem cells ; Surgery, Plastic ; Umbilical vein</subject><ispartof>Stem cell research & therapy, 2020-11, Vol.11 (1), p.496-496, Article 496</ispartof><rights>COPYRIGHT 2020 BioMed Central Ltd.</rights><rights>2020. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>The Author(s) 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c562t-88c992a8d05e7e2075d6a7cb663346200908123f69fe6a4e49b275f4bac6dcba3</citedby><cites>FETCH-LOGICAL-c562t-88c992a8d05e7e2075d6a7cb663346200908123f69fe6a4e49b275f4bac6dcba3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7687785/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7687785/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33239091$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Yao</creatorcontrib><creatorcontrib>He, Bing</creatorcontrib><creatorcontrib>Wang, Lei</creatorcontrib><creatorcontrib>Yuan, Bin</creatorcontrib><creatorcontrib>Shu, Hao</creatorcontrib><creatorcontrib>Zhang, Fucheng</creatorcontrib><creatorcontrib>Sun, Luning</creatorcontrib><title>Bone marrow mesenchymal stem cell-derived exosomes promote rotator cuff tendon-bone healing by promoting angiogenesis and regulating M1 macrophages in rats</title><title>Stem cell research & therapy</title><addtitle>Stem Cell Res Ther</addtitle><description>Rotator cuff tears (RCTs) often require reconstructive surgery. Tendon-bone healing is critical for the outcome of rotator cuff reconstruction, but the process of tendon-bone healing is complex and difficult. Mesenchymal stem cells (MSCs) are considered to be an effective method to promote tendon-bone healing. MSCs have strong paracrine, anti-inflammatory, immunoregulatory, and angiogenic potential. Recent studies have shown that MSCs achieve many regulatory functions through exosomes. The purpose of this study was to explore the role of bone marrow mesenchymal stem cell-derived exosomes (BMSC-Exos) in tendon-bone healing.
Our study found that BMSC-Exos promote the proliferation, migration, and angiogenic tube formation of human umbilical vein endothelial cells (HUVECs). The mechanism by which BMSC-Exos achieve this may be through the regulation of the angiogenic signaling pathway. In addition, BMSC-Exos can inhibit the polarization of M1 macrophages and inhibit the secretion of proinflammatory factors by M1 macrophages. After rotator cuff reconstruction in rats, BMSC-Exos were injected into the tail vein to analyze their effect on the rotator cuff tendon-bone interface healing.
It was confirmed that BMSC-Exos increased the breaking load and stiffness of the rotator cuff after reconstruction in rats, induced angiogenesis around the rotator cuff endpoint, and promoted growth of the tendon-bone interface.
BMSC-Exos promote tendon-bone healing after rotator cuff reconstruction in rats by promoting angiogenesis and inhibiting inflammation.</description><subject>Angiogenesis</subject><subject>Antibiotics</subject><subject>Antibodies</subject><subject>Bone growth</subject><subject>Bone healing</subject><subject>Bone marrow</subject><subject>Bone surgery</subject><subject>Cartilage</subject><subject>Cell proliferation</subject><subject>Collagen</subject><subject>Endothelial cells</subject><subject>Exosomes</subject><subject>Growth factors</subject><subject>Immunoregulation</subject><subject>Inflammation</subject><subject>Injuries</subject><subject>Leukocyte migration</subject><subject>Macrophages</subject><subject>Medical research</subject><subject>Mesenchymal stem cells</subject><subject>Paracrine signalling</subject><subject>Proteins</subject><subject>Reconstructive surgery</subject><subject>Rotator cuff</subject><subject>Signal transduction</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>Surgery, Plastic</subject><subject>Umbilical vein</subject><issn>1757-6512</issn><issn>1757-6512</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNptkt1u1DAQhSMEolXpC3CBLCEhuEix48RObpBKxU-lIiR-ri3HmWRdJfZiO2X7LLwsk-5SdhGJIsfxN8fOmZNlTxk9Y6wWryPjRS1zWtDloVW-eZAdM1nJXFSseLj3fpSdxnhN8eKcUlE-zo44L3hDG3ac_XrrHZBJh-B_kgkiOLO6nfRIYoKJGBjHvINgb6AjsPHRI0LWwU8-AQk-6eQDMXPfkwSu8y5vF7kV6NG6gbS3O3aZaDdYP4CDaCNOOhJgmEd9t_aJ4RFM8OuVHnAD60jQKT7JHvV6jHC6G0-y7-_ffbv4mF99_nB5cX6Vm0oUKa9r0zSFrjtagYSCyqoTWppWCM5Lgd40tGYF70XTg9AllE1byKovW21EZ1rNT7I3W9313E7QGXAp6FGtg0VfbpXXVh2uOLtSg79RUtRS1hUKvNwJBP9jhpjUZOPinXbg56iKUpSC1pxJRJ__g177OTj8PaQkYxJPzf5Sgx5BWdd73NcsoupcVFQIhixSZ_-h8O5gsgYb0Vv8flDw6qAAmQSbNOg5RnX59csh-2KPXTqaVtGPc7LexUOw2ILYvxgD9PfGMaqWqKptVBXGVN1FVW2w6Nm-5fclf4LJfwOn-uUf</recordid><startdate>20201125</startdate><enddate>20201125</enddate><creator>Huang, Yao</creator><creator>He, Bing</creator><creator>Wang, Lei</creator><creator>Yuan, Bin</creator><creator>Shu, Hao</creator><creator>Zhang, Fucheng</creator><creator>Sun, Luning</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20201125</creationdate><title>Bone marrow mesenchymal stem cell-derived exosomes promote rotator cuff tendon-bone healing by promoting angiogenesis and regulating M1 macrophages in rats</title><author>Huang, Yao ; He, Bing ; Wang, Lei ; Yuan, Bin ; Shu, Hao ; Zhang, Fucheng ; Sun, Luning</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c562t-88c992a8d05e7e2075d6a7cb663346200908123f69fe6a4e49b275f4bac6dcba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Angiogenesis</topic><topic>Antibiotics</topic><topic>Antibodies</topic><topic>Bone growth</topic><topic>Bone healing</topic><topic>Bone marrow</topic><topic>Bone surgery</topic><topic>Cartilage</topic><topic>Cell proliferation</topic><topic>Collagen</topic><topic>Endothelial cells</topic><topic>Exosomes</topic><topic>Growth factors</topic><topic>Immunoregulation</topic><topic>Inflammation</topic><topic>Injuries</topic><topic>Leukocyte migration</topic><topic>Macrophages</topic><topic>Medical research</topic><topic>Mesenchymal stem cells</topic><topic>Paracrine signalling</topic><topic>Proteins</topic><topic>Reconstructive surgery</topic><topic>Rotator cuff</topic><topic>Signal transduction</topic><topic>Stem cell transplantation</topic><topic>Stem cells</topic><topic>Surgery, Plastic</topic><topic>Umbilical vein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Yao</creatorcontrib><creatorcontrib>He, Bing</creatorcontrib><creatorcontrib>Wang, Lei</creatorcontrib><creatorcontrib>Yuan, Bin</creatorcontrib><creatorcontrib>Shu, Hao</creatorcontrib><creatorcontrib>Zhang, Fucheng</creatorcontrib><creatorcontrib>Sun, Luning</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Stem cell research & therapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Yao</au><au>He, Bing</au><au>Wang, Lei</au><au>Yuan, Bin</au><au>Shu, Hao</au><au>Zhang, Fucheng</au><au>Sun, Luning</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bone marrow mesenchymal stem cell-derived exosomes promote rotator cuff tendon-bone healing by promoting angiogenesis and regulating M1 macrophages in rats</atitle><jtitle>Stem cell research & therapy</jtitle><addtitle>Stem Cell Res Ther</addtitle><date>2020-11-25</date><risdate>2020</risdate><volume>11</volume><issue>1</issue><spage>496</spage><epage>496</epage><pages>496-496</pages><artnum>496</artnum><issn>1757-6512</issn><eissn>1757-6512</eissn><abstract>Rotator cuff tears (RCTs) often require reconstructive surgery. Tendon-bone healing is critical for the outcome of rotator cuff reconstruction, but the process of tendon-bone healing is complex and difficult. Mesenchymal stem cells (MSCs) are considered to be an effective method to promote tendon-bone healing. MSCs have strong paracrine, anti-inflammatory, immunoregulatory, and angiogenic potential. Recent studies have shown that MSCs achieve many regulatory functions through exosomes. The purpose of this study was to explore the role of bone marrow mesenchymal stem cell-derived exosomes (BMSC-Exos) in tendon-bone healing.
Our study found that BMSC-Exos promote the proliferation, migration, and angiogenic tube formation of human umbilical vein endothelial cells (HUVECs). The mechanism by which BMSC-Exos achieve this may be through the regulation of the angiogenic signaling pathway. In addition, BMSC-Exos can inhibit the polarization of M1 macrophages and inhibit the secretion of proinflammatory factors by M1 macrophages. After rotator cuff reconstruction in rats, BMSC-Exos were injected into the tail vein to analyze their effect on the rotator cuff tendon-bone interface healing.
It was confirmed that BMSC-Exos increased the breaking load and stiffness of the rotator cuff after reconstruction in rats, induced angiogenesis around the rotator cuff endpoint, and promoted growth of the tendon-bone interface.
BMSC-Exos promote tendon-bone healing after rotator cuff reconstruction in rats by promoting angiogenesis and inhibiting inflammation.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>33239091</pmid><doi>10.1186/s13287-020-02005-x</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Angiogenesis Antibiotics Antibodies Bone growth Bone healing Bone marrow Bone surgery Cartilage Cell proliferation Collagen Endothelial cells Exosomes Growth factors Immunoregulation Inflammation Injuries Leukocyte migration Macrophages Medical research Mesenchymal stem cells Paracrine signalling Proteins Reconstructive surgery Rotator cuff Signal transduction Stem cell transplantation Stem cells Surgery, Plastic Umbilical vein |
| title | Bone marrow mesenchymal stem cell-derived exosomes promote rotator cuff tendon-bone healing by promoting angiogenesis and regulating M1 macrophages in rats |
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