Stem-like Cells from Invasive Breast Carcinoma Cell Line MDA-MB-231 Express a Distinct Set of Eph Receptors and Ephrin Ligands
Background/Aim: Breast cancer cell lines consist of bulk tumor cells and a small proportion of stem-like cells. While the bulk cells are known to express a distinct combination of Eph receptors and ephrin ligands, the transcript profiles of stem-like cells in these cell lines have not been adequatel...
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| Veröffentlicht in: | Cancer genomics & proteomics 2020-11, Vol.17 (6), p.729-738 |
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| creator | LUCERO, MARIANA THIND, JASPREET SANDOVAL, JACQUELINE SENAATI, SHAYAN JIMENEZ, BELINDA KANDPAL, RAJ P. |
| description | Background/Aim: Breast cancer cell lines consist of bulk tumor cells and a small proportion of stem-like cells. While the bulk cells are known to express a distinct combination of Eph receptors and ephrin ligands, the transcript profiles of stem-like cells in these cell lines have not been adequately characterized. The aim of this study was to determine Eph receptor/ephrin ligand profiles of cancer stem cells specific to a triple negative breast carcinoma cell line. Materials and Methods: The normal breast cell line MCF10A and the invasive breast carcinoma cell line MDA-MB-231 were used to isolate CD24+/CD24− cell populations. The profiles of Eph receptors and ephrin ligands were determined by real-time PCR and the relative abundance in bulk and stem cells were compared. Results: Based on the mean ΔCT values, the descending order of abundance was as follows. Ephrin-A5 > EPHA2 > (EPHA8, EPHB2) > ephrin-B2 > (EPHA7, EPHB4, ephrin-A4) > ephrin-A3 > ephrin-A1 > (EPHB3, ephrin-B1) > EPHA4 > EPHA1 > EPHA10. EPHA6 and ephrin-A2 transcripts were not detectable in stem cells from either cell line. The expression of EPHA4, EPHA7, EPHA8, and ephrin-A5 in MDA-MB-231 stem cells was up-regulated by 12, 20, ~500, and 6.5-fold respectively. Conclusion: The up-regulation of transcripts for EPHA8 and its cognate ligand, ephrin-A5, in the stem cells isolated from MDA-MB-231, suggest their involvement in the invasiveness of this cell line. Based on literature reports, we propose the role of EPHA8 and ephrin-A5 in MDA-MB-231 stem cells via the PI3K-AKT-mTOR pathway. |
| doi_str_mv | 10.21873/cgp.20227 |
| format | Article |
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While the bulk cells are known to express a distinct combination of Eph receptors and ephrin ligands, the transcript profiles of stem-like cells in these cell lines have not been adequately characterized. The aim of this study was to determine Eph receptor/ephrin ligand profiles of cancer stem cells specific to a triple negative breast carcinoma cell line. Materials and Methods: The normal breast cell line MCF10A and the invasive breast carcinoma cell line MDA-MB-231 were used to isolate CD24+/CD24− cell populations. The profiles of Eph receptors and ephrin ligands were determined by real-time PCR and the relative abundance in bulk and stem cells were compared. Results: Based on the mean ΔCT values, the descending order of abundance was as follows. Ephrin-A5 > EPHA2 > (EPHA8, EPHB2) > ephrin-B2 > (EPHA7, EPHB4, ephrin-A4) > ephrin-A3 > ephrin-A1 > (EPHB3, ephrin-B1) > EPHA4 > EPHA1 > EPHA10. EPHA6 and ephrin-A2 transcripts were not detectable in stem cells from either cell line. The expression of EPHA4, EPHA7, EPHA8, and ephrin-A5 in MDA-MB-231 stem cells was up-regulated by 12, 20, ~500, and 6.5-fold respectively. Conclusion: The up-regulation of transcripts for EPHA8 and its cognate ligand, ephrin-A5, in the stem cells isolated from MDA-MB-231, suggest their involvement in the invasiveness of this cell line. Based on literature reports, we propose the role of EPHA8 and ephrin-A5 in MDA-MB-231 stem cells via the PI3K-AKT-mTOR pathway.</description><identifier>ISSN: 1109-6535</identifier><identifier>EISSN: 1790-6245</identifier><identifier>DOI: 10.21873/cgp.20227</identifier><identifier>PMID: 33099474</identifier><language>eng</language><publisher>Athens: International Institute of Anticancer Research</publisher><subject>1-Phosphatidylinositol 3-kinase ; AKT protein ; Biotechnology ; Breast cancer ; Breast carcinoma ; Eph protein ; EphA2 protein ; EphA4 protein ; Invasiveness ; Ligands ; Receptors ; Relative abundance ; Stem cells ; TOR protein ; Transcription ; Tumor cell lines ; Tumor cells</subject><ispartof>Cancer genomics & proteomics, 2020-11, Vol.17 (6), p.729-738</ispartof><rights>Copyright International Institute of Anticancer Research Nov/Dec 2020</rights><rights>Copyright 2020, International Institute of Anticancer Research 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c383t-9adf86362651553f08775162fc8a741a311ad516c9d47e6a05e2affb483939023</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7675649/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7675649/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids></links><search><creatorcontrib>LUCERO, MARIANA</creatorcontrib><creatorcontrib>THIND, JASPREET</creatorcontrib><creatorcontrib>SANDOVAL, JACQUELINE</creatorcontrib><creatorcontrib>SENAATI, SHAYAN</creatorcontrib><creatorcontrib>JIMENEZ, BELINDA</creatorcontrib><creatorcontrib>KANDPAL, RAJ P.</creatorcontrib><title>Stem-like Cells from Invasive Breast Carcinoma Cell Line MDA-MB-231 Express a Distinct Set of Eph Receptors and Ephrin Ligands</title><title>Cancer genomics & proteomics</title><description>Background/Aim: Breast cancer cell lines consist of bulk tumor cells and a small proportion of stem-like cells. While the bulk cells are known to express a distinct combination of Eph receptors and ephrin ligands, the transcript profiles of stem-like cells in these cell lines have not been adequately characterized. The aim of this study was to determine Eph receptor/ephrin ligand profiles of cancer stem cells specific to a triple negative breast carcinoma cell line. Materials and Methods: The normal breast cell line MCF10A and the invasive breast carcinoma cell line MDA-MB-231 were used to isolate CD24+/CD24− cell populations. The profiles of Eph receptors and ephrin ligands were determined by real-time PCR and the relative abundance in bulk and stem cells were compared. Results: Based on the mean ΔCT values, the descending order of abundance was as follows. Ephrin-A5 > EPHA2 > (EPHA8, EPHB2) > ephrin-B2 > (EPHA7, EPHB4, ephrin-A4) > ephrin-A3 > ephrin-A1 > (EPHB3, ephrin-B1) > EPHA4 > EPHA1 > EPHA10. EPHA6 and ephrin-A2 transcripts were not detectable in stem cells from either cell line. The expression of EPHA4, EPHA7, EPHA8, and ephrin-A5 in MDA-MB-231 stem cells was up-regulated by 12, 20, ~500, and 6.5-fold respectively. Conclusion: The up-regulation of transcripts for EPHA8 and its cognate ligand, ephrin-A5, in the stem cells isolated from MDA-MB-231, suggest their involvement in the invasiveness of this cell line. Based on literature reports, we propose the role of EPHA8 and ephrin-A5 in MDA-MB-231 stem cells via the PI3K-AKT-mTOR pathway.</description><subject>1-Phosphatidylinositol 3-kinase</subject><subject>AKT protein</subject><subject>Biotechnology</subject><subject>Breast cancer</subject><subject>Breast carcinoma</subject><subject>Eph protein</subject><subject>EphA2 protein</subject><subject>EphA4 protein</subject><subject>Invasiveness</subject><subject>Ligands</subject><subject>Receptors</subject><subject>Relative abundance</subject><subject>Stem cells</subject><subject>TOR protein</subject><subject>Transcription</subject><subject>Tumor cell lines</subject><subject>Tumor cells</subject><issn>1109-6535</issn><issn>1790-6245</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNpVUctOHDEQtFCi8EgufIGl3CIN-O3xJRIsG0BaFCnA2TIeezGZsSe2d0Uu-faYBUXKqburStWtLgCOMTohuJf01K7nE4IIkXvgAEuFOkEYf9d6jFQnOOX74LCUJ4SYpAx9APuUIqWYZAfgz211UzeGnw4u3DgW6HOa4HXcmhK2Dp5nZ0qFC5NtiGkyOxFchejgzcVZd3PeEYrh8nnOrhRo4EUoNURb4a2rMHm4nB_hD2fdXFNufBxekBxis1i3qXwE770Zi_v0Vo_A_bfl3eKqW32_vF6crTpLe1o7ZQbfCyqI4Jhz6lEvJceCeNsbybChGJuhAVYNTDphEHfEeP_AeqqoQoQega-vvvPmYXKDdbFmM-o5h8nk3zqZoP9nYnjU67TVUkgumGoGn98Mcvq1caXqp7TJsd2syctXGSKKN9WXV5XNqZTs_L8NGOldVrplpXdZ0b9gmoRB</recordid><startdate>20201101</startdate><enddate>20201101</enddate><creator>LUCERO, MARIANA</creator><creator>THIND, JASPREET</creator><creator>SANDOVAL, JACQUELINE</creator><creator>SENAATI, SHAYAN</creator><creator>JIMENEZ, BELINDA</creator><creator>KANDPAL, RAJ P.</creator><general>International Institute of Anticancer Research</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>20201101</creationdate><title>Stem-like Cells from Invasive Breast Carcinoma Cell Line MDA-MB-231 Express a Distinct Set of Eph Receptors and Ephrin Ligands</title><author>LUCERO, MARIANA ; THIND, JASPREET ; SANDOVAL, JACQUELINE ; SENAATI, SHAYAN ; JIMENEZ, BELINDA ; KANDPAL, RAJ P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c383t-9adf86362651553f08775162fc8a741a311ad516c9d47e6a05e2affb483939023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>1-Phosphatidylinositol 3-kinase</topic><topic>AKT protein</topic><topic>Biotechnology</topic><topic>Breast cancer</topic><topic>Breast carcinoma</topic><topic>Eph protein</topic><topic>EphA2 protein</topic><topic>EphA4 protein</topic><topic>Invasiveness</topic><topic>Ligands</topic><topic>Receptors</topic><topic>Relative abundance</topic><topic>Stem cells</topic><topic>TOR protein</topic><topic>Transcription</topic><topic>Tumor cell lines</topic><topic>Tumor cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LUCERO, MARIANA</creatorcontrib><creatorcontrib>THIND, JASPREET</creatorcontrib><creatorcontrib>SANDOVAL, JACQUELINE</creatorcontrib><creatorcontrib>SENAATI, SHAYAN</creatorcontrib><creatorcontrib>JIMENEZ, BELINDA</creatorcontrib><creatorcontrib>KANDPAL, RAJ P.</creatorcontrib><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cancer genomics & proteomics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LUCERO, MARIANA</au><au>THIND, JASPREET</au><au>SANDOVAL, JACQUELINE</au><au>SENAATI, SHAYAN</au><au>JIMENEZ, BELINDA</au><au>KANDPAL, RAJ P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stem-like Cells from Invasive Breast Carcinoma Cell Line MDA-MB-231 Express a Distinct Set of Eph Receptors and Ephrin Ligands</atitle><jtitle>Cancer genomics & proteomics</jtitle><date>2020-11-01</date><risdate>2020</risdate><volume>17</volume><issue>6</issue><spage>729</spage><epage>738</epage><pages>729-738</pages><issn>1109-6535</issn><eissn>1790-6245</eissn><abstract>Background/Aim: Breast cancer cell lines consist of bulk tumor cells and a small proportion of stem-like cells. While the bulk cells are known to express a distinct combination of Eph receptors and ephrin ligands, the transcript profiles of stem-like cells in these cell lines have not been adequately characterized. The aim of this study was to determine Eph receptor/ephrin ligand profiles of cancer stem cells specific to a triple negative breast carcinoma cell line. Materials and Methods: The normal breast cell line MCF10A and the invasive breast carcinoma cell line MDA-MB-231 were used to isolate CD24+/CD24− cell populations. The profiles of Eph receptors and ephrin ligands were determined by real-time PCR and the relative abundance in bulk and stem cells were compared. Results: Based on the mean ΔCT values, the descending order of abundance was as follows. Ephrin-A5 > EPHA2 > (EPHA8, EPHB2) > ephrin-B2 > (EPHA7, EPHB4, ephrin-A4) > ephrin-A3 > ephrin-A1 > (EPHB3, ephrin-B1) > EPHA4 > EPHA1 > EPHA10. EPHA6 and ephrin-A2 transcripts were not detectable in stem cells from either cell line. The expression of EPHA4, EPHA7, EPHA8, and ephrin-A5 in MDA-MB-231 stem cells was up-regulated by 12, 20, ~500, and 6.5-fold respectively. Conclusion: The up-regulation of transcripts for EPHA8 and its cognate ligand, ephrin-A5, in the stem cells isolated from MDA-MB-231, suggest their involvement in the invasiveness of this cell line. Based on literature reports, we propose the role of EPHA8 and ephrin-A5 in MDA-MB-231 stem cells via the PI3K-AKT-mTOR pathway.</abstract><cop>Athens</cop><pub>International Institute of Anticancer Research</pub><pmid>33099474</pmid><doi>10.21873/cgp.20227</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 1-Phosphatidylinositol 3-kinase AKT protein Biotechnology Breast cancer Breast carcinoma Eph protein EphA2 protein EphA4 protein Invasiveness Ligands Receptors Relative abundance Stem cells TOR protein Transcription Tumor cell lines Tumor cells |
| title | Stem-like Cells from Invasive Breast Carcinoma Cell Line MDA-MB-231 Express a Distinct Set of Eph Receptors and Ephrin Ligands |
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