Substrate specificity of 2-deoxy-D-ribose 5-phosphate aldolase (DERA) assessed by different protein engineering and machine learning methods

In this work, deoxyribose-5-phosphate aldolase ( Ec DERA, EC 4.1.2.4) from Escherichia coli was chosen as the protein engineering target for improving the substrate preference towards smaller, non-phosphorylated aldehyde donor substrates, in particular towards acetaldehyde. The initial broad set of...

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Veröffentlicht in:	Applied microbiology and biotechnology 2020-12, Vol.104 (24), p.10515-10529
Hauptverfasser:	Voutilainen, Sanni, Heinonen, Markus, Andberg, Martina, Jokinen, Emmi, Maaheimo, Hannu, Pääkkönen, Johan, Hakulinen, Nina, Rouvinen, Juha, Lähdesmäki, Harri, Kaski, Samuel, Rousu, Juho, Penttilä, Merja, Koivula, Anu
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetaldehyde Aldehyde-Lyases - genetics Aldehyde-Lyases - metabolism Aldehydes Aldolase Amino acids Analysis Biomedical and Life Sciences Biotechnologically Relevant Enzymes and Proteins Biotechnology D-Ribose E coli Engineering Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism Formaldehyde Fructose-Bisphosphate Aldolase - genetics Fructosediphosphate aldolase Gaussian process Glyceraldehyde Learning algorithms Life Sciences Machine Learning Methods Microbial Genetics and Genomics Microbiology Mutagenesis Mutation Protein Engineering Proteins Ribose Substrate preferences Substrate Specificity Substrates Substrates (Biochemistry)
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