A far-red cyanobacteriochrome lineage specific for verdins

Cyanobacteriochromes (CBCRs) are photoswitchable linear tetrapyrrole (bilin)-based light sensors in the phytochrome superfamily with a broad spectral range from the near UV through the far red (330 to 760 nm). The recent discovery of far-red absorbing CBCRs (frCBCRs) has garnered considerable intere...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 2020-11, Vol.117 (45), p.27962-27970
Hauptverfasser: Moreno, Marcus V., Rockwell, Nathan C., Mora, Manuel, Fisher, Andrew J., Lagarias, J. Clark
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container_title Proceedings of the National Academy of Sciences - PNAS
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creator Moreno, Marcus V.
Rockwell, Nathan C.
Mora, Manuel
Fisher, Andrew J.
Lagarias, J. Clark
description Cyanobacteriochromes (CBCRs) are photoswitchable linear tetrapyrrole (bilin)-based light sensors in the phytochrome superfamily with a broad spectral range from the near UV through the far red (330 to 760 nm). The recent discovery of far-red absorbing CBCRs (frCBCRs) has garnered considerable interest from the optogenetic and imaging communities because of the deep penetrance of farred light into mammalian tissue and the small size of the CBCR protein scaffold. The present studies were undertaken to determine the structural basis for far-red absorption by JSC1_58120g3, a frCBCR from the thermophilic cyanobacterium Leptolyngbya sp. JSC-1 that is a representative member of a phylogenetically distinct class. Unlike most CBCRs that bind phycocyanobilin (PCB), a phycobilin naturally occurring in cyanobacteria and only a few eukaryotic phototrophs, JSC1_58120g3’s far-red absorption arises from incorporation of the PCB biosynthetic intermediate 18¹,18²-dihydrobiliverdin (18¹,18²-DHBV) rather than the more reduced and more abundant PCB. JSC1_58120g3 can also yield a farred–absorbing adduct with the more widespread linear tetrapyrrole biliverdin IXα (BV), thus circumventing the need to coproduce or supplement optogenetic cell lines with PCB. Using high-resolution X-ray crystal structures of 18¹,18²-DHBV and BV adducts of JSC1_58120g3 along with structure-guided mutagenesis, we have defined residues critical for its verdin-binding preference and far-red absorption. Far-red sensing and verdin incorporation make this frCBCR lineage an attractive template for developing robust optogenetic and imaging reagents for deep tissue applications.
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Clark</creatorcontrib><title>A far-red cyanobacteriochrome lineage specific for verdins</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Cyanobacteriochromes (CBCRs) are photoswitchable linear tetrapyrrole (bilin)-based light sensors in the phytochrome superfamily with a broad spectral range from the near UV through the far red (330 to 760 nm). The recent discovery of far-red absorbing CBCRs (frCBCRs) has garnered considerable interest from the optogenetic and imaging communities because of the deep penetrance of farred light into mammalian tissue and the small size of the CBCR protein scaffold. The present studies were undertaken to determine the structural basis for far-red absorption by JSC1_58120g3, a frCBCR from the thermophilic cyanobacterium Leptolyngbya sp. JSC-1 that is a representative member of a phylogenetically distinct class. Unlike most CBCRs that bind phycocyanobilin (PCB), a phycobilin naturally occurring in cyanobacteria and only a few eukaryotic phototrophs, JSC1_58120g3’s far-red absorption arises from incorporation of the PCB biosynthetic intermediate 18¹,18²-dihydrobiliverdin (18¹,18²-DHBV) rather than the more reduced and more abundant PCB. JSC1_58120g3 can also yield a farred–absorbing adduct with the more widespread linear tetrapyrrole biliverdin IXα (BV), thus circumventing the need to coproduce or supplement optogenetic cell lines with PCB. Using high-resolution X-ray crystal structures of 18¹,18²-DHBV and BV adducts of JSC1_58120g3 along with structure-guided mutagenesis, we have defined residues critical for its verdin-binding preference and far-red absorption. 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Clark</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A far-red cyanobacteriochrome lineage specific for verdins</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>2020-11-10</date><risdate>2020</risdate><volume>117</volume><issue>45</issue><spage>27962</spage><epage>27970</epage><pages>27962-27970</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Cyanobacteriochromes (CBCRs) are photoswitchable linear tetrapyrrole (bilin)-based light sensors in the phytochrome superfamily with a broad spectral range from the near UV through the far red (330 to 760 nm). The recent discovery of far-red absorbing CBCRs (frCBCRs) has garnered considerable interest from the optogenetic and imaging communities because of the deep penetrance of farred light into mammalian tissue and the small size of the CBCR protein scaffold. 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Using high-resolution X-ray crystal structures of 18¹,18²-DHBV and BV adducts of JSC1_58120g3 along with structure-guided mutagenesis, we have defined residues critical for its verdin-binding preference and far-red absorption. 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subjects Absorption
Adducts
Bacterial Proteins - metabolism
Biliverdin
Biliverdine - chemistry
Biological Sciences
Cell lines
Crystal structure
Cyanobacteria
Cyanobacteria - genetics
Cyanobacteria - metabolism
Light
Mutagenesis
Photoreceptor Cells - metabolism
Photoreceptors, Microbial - chemistry
Phycobilin
Phycobilins - genetics
Phycobilins - metabolism
Phycocyanin - genetics
Phycocyanin - metabolism
Phycocyanobilin
Phylogeny
Physical Sciences
Phytochrome - genetics
Phytochrome - metabolism
Porphyrins - genetics
Porphyrins - metabolism
Reagents
title A far-red cyanobacteriochrome lineage specific for verdins
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