The Mechanism of Bisdemethoxycurcumin Enhances Conventional Antibiotics against Methicillin-Resistant Staphylococcus aureus
Methicillin-resistant (MRSA) infection has posed a serious threat to public health, therefore, the development of new antibacterial drugs is imperative. Bisdemethoxycurcumin (BDMC) is a curcumin analog that exists in nature and possesses extensive pharmacological actions. This review focuses on inve...
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| Veröffentlicht in: | International journal of molecular sciences 2020-10, Vol.21 (21), p.7945 |
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| creator | Wang, Shu Kim, Min-Chul Kang, Ok-Hwa Kwon, Dong-Yeul |
| description | Methicillin-resistant
(MRSA) infection has posed a serious threat to public health, therefore, the development of new antibacterial drugs is imperative. Bisdemethoxycurcumin (BDMC) is a curcumin analog that exists in nature and possesses extensive pharmacological actions. This review focuses on investigating the antibacterial activity of BDMC alone or in combination with three antibiotics against MRSA. We determined the minimal inhibitory concentration of BDMC, with a broth microdilution assay, and the value against all six strains was 7.8 μg/mL. The synergistic effect of BDMC combined with the antibiotics was determined using a checkerboard dilution test and a time-kill curve assay. The results showed that the antimicrobial effect of BDMC combined with antibiotics was superior to treatment with that of a single agent alone. We examined the antibacterial activity of BDMC in the presence of a membrane-permeabilizing agent and an ATPase-inhibiting agent, respectively. In addition, we analyzed the
transcription gene and the penicillin-binding protein 2a (PBP2a) level of MRSA treated with BDMC by quantitative RT-PCR or Western blot assay. The gene transcription and the protein level were significantly inhibited. This study demonstrated that BDMC has potent antibacterial activity, and proved that BDMC may be a potential natural modulator of antibiotics. |
| doi_str_mv | 10.3390/ijms21217945 |
| format | Article |
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(MRSA) infection has posed a serious threat to public health, therefore, the development of new antibacterial drugs is imperative. Bisdemethoxycurcumin (BDMC) is a curcumin analog that exists in nature and possesses extensive pharmacological actions. This review focuses on investigating the antibacterial activity of BDMC alone or in combination with three antibiotics against MRSA. We determined the minimal inhibitory concentration of BDMC, with a broth microdilution assay, and the value against all six strains was 7.8 μg/mL. The synergistic effect of BDMC combined with the antibiotics was determined using a checkerboard dilution test and a time-kill curve assay. The results showed that the antimicrobial effect of BDMC combined with antibiotics was superior to treatment with that of a single agent alone. We examined the antibacterial activity of BDMC in the presence of a membrane-permeabilizing agent and an ATPase-inhibiting agent, respectively. In addition, we analyzed the
transcription gene and the penicillin-binding protein 2a (PBP2a) level of MRSA treated with BDMC by quantitative RT-PCR or Western blot assay. The gene transcription and the protein level were significantly inhibited. This study demonstrated that BDMC has potent antibacterial activity, and proved that BDMC may be a potential natural modulator of antibiotics.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms21217945</identifier><identifier>PMID: 33114703</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Adenosine triphosphatase ; Ampicillin - pharmacology ; Anti-Bacterial Agents - pharmacology ; Antibacterial activity ; Antibiotics ; Antimicrobial agents ; Assaying ; Bacterial infections ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Curcumin ; Diarylheptanoids - pharmacology ; Dilution ; Down-Regulation ; Drug development ; Drug resistance ; Drug Synergism ; Gene Expression Regulation, Bacterial - drug effects ; MecA protein ; Methicillin ; Methicillin-Resistant Staphylococcus aureus - drug effects ; Methicillin-Resistant Staphylococcus aureus - genetics ; Methicillin-Resistant Staphylococcus aureus - metabolism ; Microbial Sensitivity Tests ; Nosocomial infections ; Oxacillin - pharmacology ; Penicillin ; Penicillin-binding protein ; Penicillin-binding protein 2a ; Penicillin-Binding Proteins - genetics ; Penicillin-Binding Proteins - metabolism ; Permeability ; Polymerase chain reaction ; Protein expression ; Proteins ; Public health ; Reagents ; Signal transduction ; Staphylococcus aureus ; Staphylococcus infections ; Synergistic effect ; Transcription</subject><ispartof>International journal of molecular sciences, 2020-10, Vol.21 (21), p.7945</ispartof><rights>2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 by the authors. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-678f21abec0525871ad77ddafd2d79b754527cb81e46379bc46acb70cff4b0e53</citedby><cites>FETCH-LOGICAL-c412t-678f21abec0525871ad77ddafd2d79b754527cb81e46379bc46acb70cff4b0e53</cites><orcidid>0000-0002-6885-1499</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7662602/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7662602/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33114703$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Shu</creatorcontrib><creatorcontrib>Kim, Min-Chul</creatorcontrib><creatorcontrib>Kang, Ok-Hwa</creatorcontrib><creatorcontrib>Kwon, Dong-Yeul</creatorcontrib><title>The Mechanism of Bisdemethoxycurcumin Enhances Conventional Antibiotics against Methicillin-Resistant Staphylococcus aureus</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>Methicillin-resistant
(MRSA) infection has posed a serious threat to public health, therefore, the development of new antibacterial drugs is imperative. Bisdemethoxycurcumin (BDMC) is a curcumin analog that exists in nature and possesses extensive pharmacological actions. This review focuses on investigating the antibacterial activity of BDMC alone or in combination with three antibiotics against MRSA. We determined the minimal inhibitory concentration of BDMC, with a broth microdilution assay, and the value against all six strains was 7.8 μg/mL. The synergistic effect of BDMC combined with the antibiotics was determined using a checkerboard dilution test and a time-kill curve assay. The results showed that the antimicrobial effect of BDMC combined with antibiotics was superior to treatment with that of a single agent alone. We examined the antibacterial activity of BDMC in the presence of a membrane-permeabilizing agent and an ATPase-inhibiting agent, respectively. In addition, we analyzed the
transcription gene and the penicillin-binding protein 2a (PBP2a) level of MRSA treated with BDMC by quantitative RT-PCR or Western blot assay. The gene transcription and the protein level were significantly inhibited. This study demonstrated that BDMC has potent antibacterial activity, and proved that BDMC may be a potential natural modulator of antibiotics.</description><subject>Adenosine triphosphatase</subject><subject>Ampicillin - pharmacology</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibacterial activity</subject><subject>Antibiotics</subject><subject>Antimicrobial agents</subject><subject>Assaying</subject><subject>Bacterial infections</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Curcumin</subject><subject>Diarylheptanoids - pharmacology</subject><subject>Dilution</subject><subject>Down-Regulation</subject><subject>Drug development</subject><subject>Drug resistance</subject><subject>Drug Synergism</subject><subject>Gene Expression Regulation, Bacterial - drug effects</subject><subject>MecA protein</subject><subject>Methicillin</subject><subject>Methicillin-Resistant Staphylococcus aureus - drug effects</subject><subject>Methicillin-Resistant Staphylococcus aureus - genetics</subject><subject>Methicillin-Resistant Staphylococcus aureus - metabolism</subject><subject>Microbial Sensitivity Tests</subject><subject>Nosocomial infections</subject><subject>Oxacillin - pharmacology</subject><subject>Penicillin</subject><subject>Penicillin-binding protein</subject><subject>Penicillin-binding protein 2a</subject><subject>Penicillin-Binding Proteins - genetics</subject><subject>Penicillin-Binding Proteins - metabolism</subject><subject>Permeability</subject><subject>Polymerase chain reaction</subject><subject>Protein expression</subject><subject>Proteins</subject><subject>Public health</subject><subject>Reagents</subject><subject>Signal transduction</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus infections</subject><subject>Synergistic effect</subject><subject>Transcription</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpVkc1r3DAQxUVp6Cbb3nouhl7rRJ_W-lJIls0HJATS9CzksbzWYktbSV665J-PStKwOc0w8-PNYx5CXwk-ZazGZ3YzRkookTUXH9Ax4ZSWGFfy40E_QycxbjCmjIr6E5oxRgiXmB2jp8feFHcGeu1sHAvfFRc2tmY0qfd_9zAFmEbripXLAJhYLL3bGZesd3ooznPTWJ8sxEKvtXUxZa3UW7DDYF35YKKNSbtU_Ep62-8HDx5gyvAUzBQ_o6NOD9F8ea1z9Pty9bi8Lm_vr26W57clcEJTWclFR4luDGBBxUIS3UrZtrpraSvrRgouqIRmQQyvWB4ArzQ0EkPX8QYbwebo54vudmpG00L2H_SgtsGOOuyV11a93zjbq7XfKVlVtMpPm6PvrwLB_5lMTGrjp5A_EBUVfCFqQeo6Uz9eKAg-xmC6twsEq39RqcOoMv7t0NUb_D8b9gwYDZRk</recordid><startdate>20201026</startdate><enddate>20201026</enddate><creator>Wang, Shu</creator><creator>Kim, Min-Chul</creator><creator>Kang, Ok-Hwa</creator><creator>Kwon, Dong-Yeul</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-6885-1499</orcidid></search><sort><creationdate>20201026</creationdate><title>The Mechanism of Bisdemethoxycurcumin Enhances Conventional Antibiotics against Methicillin-Resistant Staphylococcus aureus</title><author>Wang, Shu ; Kim, Min-Chul ; Kang, Ok-Hwa ; Kwon, Dong-Yeul</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-678f21abec0525871ad77ddafd2d79b754527cb81e46379bc46acb70cff4b0e53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adenosine triphosphatase</topic><topic>Ampicillin - pharmacology</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibacterial activity</topic><topic>Antibiotics</topic><topic>Antimicrobial agents</topic><topic>Assaying</topic><topic>Bacterial infections</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Curcumin</topic><topic>Diarylheptanoids - pharmacology</topic><topic>Dilution</topic><topic>Down-Regulation</topic><topic>Drug development</topic><topic>Drug resistance</topic><topic>Drug Synergism</topic><topic>Gene Expression Regulation, Bacterial - drug effects</topic><topic>MecA protein</topic><topic>Methicillin</topic><topic>Methicillin-Resistant Staphylococcus aureus - drug effects</topic><topic>Methicillin-Resistant Staphylococcus aureus - genetics</topic><topic>Methicillin-Resistant Staphylococcus aureus - metabolism</topic><topic>Microbial Sensitivity Tests</topic><topic>Nosocomial infections</topic><topic>Oxacillin - pharmacology</topic><topic>Penicillin</topic><topic>Penicillin-binding protein</topic><topic>Penicillin-binding protein 2a</topic><topic>Penicillin-Binding Proteins - genetics</topic><topic>Penicillin-Binding Proteins - metabolism</topic><topic>Permeability</topic><topic>Polymerase chain reaction</topic><topic>Protein expression</topic><topic>Proteins</topic><topic>Public health</topic><topic>Reagents</topic><topic>Signal transduction</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus infections</topic><topic>Synergistic effect</topic><topic>Transcription</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Shu</creatorcontrib><creatorcontrib>Kim, Min-Chul</creatorcontrib><creatorcontrib>Kang, Ok-Hwa</creatorcontrib><creatorcontrib>Kwon, Dong-Yeul</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Shu</au><au>Kim, Min-Chul</au><au>Kang, Ok-Hwa</au><au>Kwon, Dong-Yeul</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Mechanism of Bisdemethoxycurcumin Enhances Conventional Antibiotics against Methicillin-Resistant Staphylococcus aureus</atitle><jtitle>International journal of molecular sciences</jtitle><addtitle>Int J Mol Sci</addtitle><date>2020-10-26</date><risdate>2020</risdate><volume>21</volume><issue>21</issue><spage>7945</spage><pages>7945-</pages><issn>1422-0067</issn><issn>1661-6596</issn><eissn>1422-0067</eissn><abstract>Methicillin-resistant
(MRSA) infection has posed a serious threat to public health, therefore, the development of new antibacterial drugs is imperative. Bisdemethoxycurcumin (BDMC) is a curcumin analog that exists in nature and possesses extensive pharmacological actions. This review focuses on investigating the antibacterial activity of BDMC alone or in combination with three antibiotics against MRSA. We determined the minimal inhibitory concentration of BDMC, with a broth microdilution assay, and the value against all six strains was 7.8 μg/mL. The synergistic effect of BDMC combined with the antibiotics was determined using a checkerboard dilution test and a time-kill curve assay. The results showed that the antimicrobial effect of BDMC combined with antibiotics was superior to treatment with that of a single agent alone. We examined the antibacterial activity of BDMC in the presence of a membrane-permeabilizing agent and an ATPase-inhibiting agent, respectively. In addition, we analyzed the
transcription gene and the penicillin-binding protein 2a (PBP2a) level of MRSA treated with BDMC by quantitative RT-PCR or Western blot assay. The gene transcription and the protein level were significantly inhibited. This study demonstrated that BDMC has potent antibacterial activity, and proved that BDMC may be a potential natural modulator of antibiotics.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>33114703</pmid><doi>10.3390/ijms21217945</doi><orcidid>https://orcid.org/0000-0002-6885-1499</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Adenosine triphosphatase Ampicillin - pharmacology Anti-Bacterial Agents - pharmacology Antibacterial activity Antibiotics Antimicrobial agents Assaying Bacterial infections Bacterial Proteins - genetics Bacterial Proteins - metabolism Curcumin Diarylheptanoids - pharmacology Dilution Down-Regulation Drug development Drug resistance Drug Synergism Gene Expression Regulation, Bacterial - drug effects MecA protein Methicillin Methicillin-Resistant Staphylococcus aureus - drug effects Methicillin-Resistant Staphylococcus aureus - genetics Methicillin-Resistant Staphylococcus aureus - metabolism Microbial Sensitivity Tests Nosocomial infections Oxacillin - pharmacology Penicillin Penicillin-binding protein Penicillin-binding protein 2a Penicillin-Binding Proteins - genetics Penicillin-Binding Proteins - metabolism Permeability Polymerase chain reaction Protein expression Proteins Public health Reagents Signal transduction Staphylococcus aureus Staphylococcus infections Synergistic effect Transcription |
| title | The Mechanism of Bisdemethoxycurcumin Enhances Conventional Antibiotics against Methicillin-Resistant Staphylococcus aureus |
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