Histological and prognostic importance of CD44+/CD24+/EpCAM+ expression in clinical pancreatic cancer
CD44+/CD24+/EpCAM+ cells have been reported to be cancer stem cells in pancreatic cancer; however, the histological and clinical importance of these cells has not yet been investigated. Here we clarified the characteristics of CD44+/CD24+/EpCAM+ cells in clinical specimens of pancreatic cancer using...
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| Veröffentlicht in: | Cancer science 2013-08, Vol.104 (8), p.1127-1134 |
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| creator | Ohara, Yusuke Oda, Tatsuya Sugano, Masato Hashimoto, Shinji Enomoto, Tsuyoshi Yamada, Keiichi Akashi, Yoshimasa Miyamoto, Ryoichi Kobayashi, Akihiko Fukunaga, Kiyoshi Morishita, Yukio Ohkohchi, Nobuhiro |
| description | CD44+/CD24+/EpCAM+ cells have been reported to be cancer stem cells in pancreatic cancer; however, the histological and clinical importance of these cells has not yet been investigated. Here we clarified the characteristics of CD44+/CD24+/EpCAM+ cells in clinical specimens of pancreatic cancer using immunohistochemical assay. We used surgical specimens of pancreatic ductal adenocarcinoma from 101 patients. In view of tumor heterogeneity, we randomly selected 10 high‐power fields per case, and triple‐positive CD44+/CD24+/EpCAM+ expression was identified using our scoring system. The distribution, histological characteristics, and prognostic importance of CD44+/CD24+/EpCAM+ cells were then analyzed. As a result, the distribution of CD44+/CD24+/EpCAM+ cells varied widely among the 101 cases examined, and CD44+/CD24+/EpCAM+ expression was correlated with poor glandular differentiation and high proliferation. Survival analysis showed that CD44+/CD24+/EpCAM+ expression was not correlated with patient outcome; however, CD44+/CD24+ expression appeared to be correlated with poor prognosis. In conclusion, CD44+/CD24+/EpCAM+ expression overlapped with poorly differentiated cells and possessed high proliferative potential in clinical pancreatic cancer. In particular, the presence of double‐positive CD44+/CD24+ expression seemed to have clinical relevance, associating with poor prognosis. |
| doi_str_mv | 10.1111/cas.12198 |
| format | Article |
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Here we clarified the characteristics of CD44+/CD24+/EpCAM+ cells in clinical specimens of pancreatic cancer using immunohistochemical assay. We used surgical specimens of pancreatic ductal adenocarcinoma from 101 patients. In view of tumor heterogeneity, we randomly selected 10 high‐power fields per case, and triple‐positive CD44+/CD24+/EpCAM+ expression was identified using our scoring system. The distribution, histological characteristics, and prognostic importance of CD44+/CD24+/EpCAM+ cells were then analyzed. As a result, the distribution of CD44+/CD24+/EpCAM+ cells varied widely among the 101 cases examined, and CD44+/CD24+/EpCAM+ expression was correlated with poor glandular differentiation and high proliferation. Survival analysis showed that CD44+/CD24+/EpCAM+ expression was not correlated with patient outcome; however, CD44+/CD24+ expression appeared to be correlated with poor prognosis. In conclusion, CD44+/CD24+/EpCAM+ expression overlapped with poorly differentiated cells and possessed high proliferative potential in clinical pancreatic cancer. In particular, the presence of double‐positive CD44+/CD24+ expression seemed to have clinical relevance, associating with poor prognosis.</description><identifier>ISSN: 1347-9032</identifier><identifier>EISSN: 1349-7006</identifier><identifier>DOI: 10.1111/cas.12198</identifier><identifier>PMID: 23679813</identifier><language>eng</language><publisher>England: John Wiley and Sons Inc</publisher><subject>Adenocarcinoma - genetics ; Adenocarcinoma - metabolism ; Adenocarcinoma - pathology ; Adult ; Aged ; Aged, 80 and over ; Antigens, Neoplasm - biosynthesis ; Antigens, Neoplasm - genetics ; Antigens, Neoplasm - metabolism ; Carcinoma, Pancreatic Ductal - genetics ; Carcinoma, Pancreatic Ductal - metabolism ; Carcinoma, Pancreatic Ductal - pathology ; CD24 Antigen - biosynthesis ; CD24 Antigen - genetics ; CD24 Antigen - metabolism ; Cell Adhesion Molecules - biosynthesis ; Cell Adhesion Molecules - genetics ; Cell Adhesion Molecules - metabolism ; Cell Differentiation - genetics ; Cell Growth Processes - genetics ; Epithelial Cell Adhesion Molecule ; Female ; Humans ; Hyaluronan Receptors - biosynthesis ; Hyaluronan Receptors - genetics ; Hyaluronan Receptors - metabolism ; Male ; Middle Aged ; Original ; Pancreatic Neoplasms - genetics ; Pancreatic Neoplasms - metabolism ; Pancreatic Neoplasms - pathology ; Prognosis ; Survival Analysis</subject><ispartof>Cancer science, 2013-08, Vol.104 (8), p.1127-1134</ispartof><rights>2013 Japanese Cancer Association</rights><rights>2013 Japanese Cancer Association.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7657217/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7657217/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,1417,11562,27924,27925,45574,45575,46052,46476,53791,53793</link.rule.ids><linktorsrc>$$Uhttps://onlinelibrary.wiley.com/doi/abs/10.1111%2Fcas.12198$$EView_record_in_Wiley-Blackwell$$FView_record_in_$$GWiley-Blackwell</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23679813$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ohara, Yusuke</creatorcontrib><creatorcontrib>Oda, Tatsuya</creatorcontrib><creatorcontrib>Sugano, Masato</creatorcontrib><creatorcontrib>Hashimoto, Shinji</creatorcontrib><creatorcontrib>Enomoto, Tsuyoshi</creatorcontrib><creatorcontrib>Yamada, Keiichi</creatorcontrib><creatorcontrib>Akashi, Yoshimasa</creatorcontrib><creatorcontrib>Miyamoto, Ryoichi</creatorcontrib><creatorcontrib>Kobayashi, Akihiko</creatorcontrib><creatorcontrib>Fukunaga, Kiyoshi</creatorcontrib><creatorcontrib>Morishita, Yukio</creatorcontrib><creatorcontrib>Ohkohchi, Nobuhiro</creatorcontrib><title>Histological and prognostic importance of CD44+/CD24+/EpCAM+ expression in clinical pancreatic cancer</title><title>Cancer science</title><addtitle>Cancer Sci</addtitle><description>CD44+/CD24+/EpCAM+ cells have been reported to be cancer stem cells in pancreatic cancer; however, the histological and clinical importance of these cells has not yet been investigated. Here we clarified the characteristics of CD44+/CD24+/EpCAM+ cells in clinical specimens of pancreatic cancer using immunohistochemical assay. We used surgical specimens of pancreatic ductal adenocarcinoma from 101 patients. In view of tumor heterogeneity, we randomly selected 10 high‐power fields per case, and triple‐positive CD44+/CD24+/EpCAM+ expression was identified using our scoring system. The distribution, histological characteristics, and prognostic importance of CD44+/CD24+/EpCAM+ cells were then analyzed. As a result, the distribution of CD44+/CD24+/EpCAM+ cells varied widely among the 101 cases examined, and CD44+/CD24+/EpCAM+ expression was correlated with poor glandular differentiation and high proliferation. Survival analysis showed that CD44+/CD24+/EpCAM+ expression was not correlated with patient outcome; however, CD44+/CD24+ expression appeared to be correlated with poor prognosis. In conclusion, CD44+/CD24+/EpCAM+ expression overlapped with poorly differentiated cells and possessed high proliferative potential in clinical pancreatic cancer. In particular, the presence of double‐positive CD44+/CD24+ expression seemed to have clinical relevance, associating with poor prognosis.</description><subject>Adenocarcinoma - genetics</subject><subject>Adenocarcinoma - metabolism</subject><subject>Adenocarcinoma - pathology</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Antigens, Neoplasm - biosynthesis</subject><subject>Antigens, Neoplasm - genetics</subject><subject>Antigens, Neoplasm - metabolism</subject><subject>Carcinoma, Pancreatic Ductal - genetics</subject><subject>Carcinoma, Pancreatic Ductal - metabolism</subject><subject>Carcinoma, Pancreatic Ductal - pathology</subject><subject>CD24 Antigen - biosynthesis</subject><subject>CD24 Antigen - genetics</subject><subject>CD24 Antigen - metabolism</subject><subject>Cell Adhesion Molecules - biosynthesis</subject><subject>Cell Adhesion Molecules - genetics</subject><subject>Cell Adhesion Molecules - metabolism</subject><subject>Cell Differentiation - genetics</subject><subject>Cell Growth Processes - genetics</subject><subject>Epithelial Cell Adhesion Molecule</subject><subject>Female</subject><subject>Humans</subject><subject>Hyaluronan Receptors - biosynthesis</subject><subject>Hyaluronan Receptors - genetics</subject><subject>Hyaluronan Receptors - metabolism</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Original</subject><subject>Pancreatic Neoplasms - genetics</subject><subject>Pancreatic Neoplasms - metabolism</subject><subject>Pancreatic Neoplasms - pathology</subject><subject>Prognosis</subject><subject>Survival Analysis</subject><issn>1347-9032</issn><issn>1349-7006</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkUtLAzEQx4Mo1tfBLyB7FMrazaOb5CKUbX2A4kE9h2yS1pRtsiZbH9_e9GHROcwMzH9-A_MH4BwWVzDFQMl4BRHkbA8cQUx4Toui3F_3NOcFRj1wHOO8KHBJODkEPYRLyhnER8Dc2dj5xs-skk0mnc7a4GfOx86qzC5aHzrplMn8NKvGhPQH1RilPGmr0WM_M19tMDFa7zLrMtVYt8a0aSUYuUKo1XY4BQdT2URztq0n4PVm8lLd5Q9Pt_fV6CGfEwxZDhXmZaHZUCFqWI0kLDliPFWEEGNac8lMyXRtao0ox2U9VLXWiEtNYEr4BFxvuO2yXhitjOuCbEQb7EKGb-GlFf8nzr6Jmf8QtBxSBGkCXG4Bwb8vTezEwkZlmkY645dRQAIZJJSSYZJe_L21O_L72yQYbASftjHfuzksxMo0kUwTa9NENXpeN_gHCseJsg</recordid><startdate>201308</startdate><enddate>201308</enddate><creator>Ohara, Yusuke</creator><creator>Oda, Tatsuya</creator><creator>Sugano, Masato</creator><creator>Hashimoto, Shinji</creator><creator>Enomoto, Tsuyoshi</creator><creator>Yamada, Keiichi</creator><creator>Akashi, Yoshimasa</creator><creator>Miyamoto, Ryoichi</creator><creator>Kobayashi, Akihiko</creator><creator>Fukunaga, Kiyoshi</creator><creator>Morishita, Yukio</creator><creator>Ohkohchi, Nobuhiro</creator><general>John Wiley and Sons Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201308</creationdate><title>Histological and prognostic importance of CD44+/CD24+/EpCAM+ expression in clinical pancreatic cancer</title><author>Ohara, Yusuke ; Oda, Tatsuya ; Sugano, Masato ; Hashimoto, Shinji ; Enomoto, Tsuyoshi ; Yamada, Keiichi ; Akashi, Yoshimasa ; Miyamoto, Ryoichi ; Kobayashi, Akihiko ; Fukunaga, Kiyoshi ; Morishita, Yukio ; Ohkohchi, Nobuhiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j4318-1c3960d85c27e8b2a1692892a122288dd9a8e68dbebd27936b5cbdd29ad419ad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adenocarcinoma - genetics</topic><topic>Adenocarcinoma - metabolism</topic><topic>Adenocarcinoma - pathology</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Antigens, Neoplasm - biosynthesis</topic><topic>Antigens, Neoplasm - genetics</topic><topic>Antigens, Neoplasm - metabolism</topic><topic>Carcinoma, Pancreatic Ductal - genetics</topic><topic>Carcinoma, Pancreatic Ductal - metabolism</topic><topic>Carcinoma, Pancreatic Ductal - pathology</topic><topic>CD24 Antigen - biosynthesis</topic><topic>CD24 Antigen - genetics</topic><topic>CD24 Antigen - metabolism</topic><topic>Cell Adhesion Molecules - biosynthesis</topic><topic>Cell Adhesion Molecules - genetics</topic><topic>Cell Adhesion Molecules - metabolism</topic><topic>Cell Differentiation - genetics</topic><topic>Cell Growth Processes - genetics</topic><topic>Epithelial Cell Adhesion Molecule</topic><topic>Female</topic><topic>Humans</topic><topic>Hyaluronan Receptors - biosynthesis</topic><topic>Hyaluronan Receptors - genetics</topic><topic>Hyaluronan Receptors - metabolism</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Original</topic><topic>Pancreatic Neoplasms - genetics</topic><topic>Pancreatic Neoplasms - metabolism</topic><topic>Pancreatic Neoplasms - pathology</topic><topic>Prognosis</topic><topic>Survival Analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ohara, Yusuke</creatorcontrib><creatorcontrib>Oda, Tatsuya</creatorcontrib><creatorcontrib>Sugano, Masato</creatorcontrib><creatorcontrib>Hashimoto, Shinji</creatorcontrib><creatorcontrib>Enomoto, Tsuyoshi</creatorcontrib><creatorcontrib>Yamada, Keiichi</creatorcontrib><creatorcontrib>Akashi, Yoshimasa</creatorcontrib><creatorcontrib>Miyamoto, Ryoichi</creatorcontrib><creatorcontrib>Kobayashi, Akihiko</creatorcontrib><creatorcontrib>Fukunaga, Kiyoshi</creatorcontrib><creatorcontrib>Morishita, Yukio</creatorcontrib><creatorcontrib>Ohkohchi, Nobuhiro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cancer science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Ohara, Yusuke</au><au>Oda, Tatsuya</au><au>Sugano, Masato</au><au>Hashimoto, Shinji</au><au>Enomoto, Tsuyoshi</au><au>Yamada, Keiichi</au><au>Akashi, Yoshimasa</au><au>Miyamoto, Ryoichi</au><au>Kobayashi, Akihiko</au><au>Fukunaga, Kiyoshi</au><au>Morishita, Yukio</au><au>Ohkohchi, Nobuhiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Histological and prognostic importance of CD44+/CD24+/EpCAM+ expression in clinical pancreatic cancer</atitle><jtitle>Cancer science</jtitle><addtitle>Cancer Sci</addtitle><date>2013-08</date><risdate>2013</risdate><volume>104</volume><issue>8</issue><spage>1127</spage><epage>1134</epage><pages>1127-1134</pages><issn>1347-9032</issn><eissn>1349-7006</eissn><abstract>CD44+/CD24+/EpCAM+ cells have been reported to be cancer stem cells in pancreatic cancer; however, the histological and clinical importance of these cells has not yet been investigated. Here we clarified the characteristics of CD44+/CD24+/EpCAM+ cells in clinical specimens of pancreatic cancer using immunohistochemical assay. We used surgical specimens of pancreatic ductal adenocarcinoma from 101 patients. In view of tumor heterogeneity, we randomly selected 10 high‐power fields per case, and triple‐positive CD44+/CD24+/EpCAM+ expression was identified using our scoring system. The distribution, histological characteristics, and prognostic importance of CD44+/CD24+/EpCAM+ cells were then analyzed. As a result, the distribution of CD44+/CD24+/EpCAM+ cells varied widely among the 101 cases examined, and CD44+/CD24+/EpCAM+ expression was correlated with poor glandular differentiation and high proliferation. Survival analysis showed that CD44+/CD24+/EpCAM+ expression was not correlated with patient outcome; however, CD44+/CD24+ expression appeared to be correlated with poor prognosis. In conclusion, CD44+/CD24+/EpCAM+ expression overlapped with poorly differentiated cells and possessed high proliferative potential in clinical pancreatic cancer. In particular, the presence of double‐positive CD44+/CD24+ expression seemed to have clinical relevance, associating with poor prognosis.</abstract><cop>England</cop><pub>John Wiley and Sons Inc</pub><pmid>23679813</pmid><doi>10.1111/cas.12198</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adenocarcinoma - genetics Adenocarcinoma - metabolism Adenocarcinoma - pathology Adult Aged Aged, 80 and over Antigens, Neoplasm - biosynthesis Antigens, Neoplasm - genetics Antigens, Neoplasm - metabolism Carcinoma, Pancreatic Ductal - genetics Carcinoma, Pancreatic Ductal - metabolism Carcinoma, Pancreatic Ductal - pathology CD24 Antigen - biosynthesis CD24 Antigen - genetics CD24 Antigen - metabolism Cell Adhesion Molecules - biosynthesis Cell Adhesion Molecules - genetics Cell Adhesion Molecules - metabolism Cell Differentiation - genetics Cell Growth Processes - genetics Epithelial Cell Adhesion Molecule Female Humans Hyaluronan Receptors - biosynthesis Hyaluronan Receptors - genetics Hyaluronan Receptors - metabolism Male Middle Aged Original Pancreatic Neoplasms - genetics Pancreatic Neoplasms - metabolism Pancreatic Neoplasms - pathology Prognosis Survival Analysis |
| title | Histological and prognostic importance of CD44+/CD24+/EpCAM+ expression in clinical pancreatic cancer |
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