Immunohistochemical analysis of periostin in the hearts of Lewis rats with experimental autoimmune myocarditis
Periostin plays a critical role in tissue regeneration and homeostasis. The aim of this study was to evaluate the changes in periostin levels in the hearts of rats with experimental autoimmune myocarditis (EAM). Western blot analysis revealed that the expression levels of periostin and alpha-smooth...
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| Veröffentlicht in: | Journal of Veterinary Medical Science 2020, Vol.82(10), pp.1545-1550 |
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| creator | CHOI, Yuna OH, Hanseul AHN, Meejung KANG, Taeyoung CHUN, Jiyoon SHIN, Taekyun KIM, Jeongtae |
| description | Periostin plays a critical role in tissue regeneration and homeostasis. The aim of this study was to evaluate the changes in periostin levels in the hearts of rats with experimental autoimmune myocarditis (EAM). Western blot analysis revealed that the expression levels of periostin and alpha-smooth muscle actin were significantly increased at day 14 post-immunization. Immunohistochemical analysis indicated that periostin was expressed in macrophages and fibroblasts in the hearts of EAM-induced rats. In conclusion, these results suggest that increased periostin expression in macrophages and fibroblasts promotes cardiac fibrosis in EAM-induced rats, potentially by enhancing immune cell infiltration. Therefore, periostin should be further investigated as a candidate therapeutic target for myocarditis. |
| doi_str_mv | 10.1292/jvms.20-0225 |
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The aim of this study was to evaluate the changes in periostin levels in the hearts of rats with experimental autoimmune myocarditis (EAM). Western blot analysis revealed that the expression levels of periostin and alpha-smooth muscle actin were significantly increased at day 14 post-immunization. Immunohistochemical analysis indicated that periostin was expressed in macrophages and fibroblasts in the hearts of EAM-induced rats. In conclusion, these results suggest that increased periostin expression in macrophages and fibroblasts promotes cardiac fibrosis in EAM-induced rats, potentially by enhancing immune cell infiltration. Therefore, periostin should be further investigated as a candidate therapeutic target for myocarditis.</description><identifier>ISSN: 0916-7250</identifier><identifier>EISSN: 1347-7439</identifier><identifier>DOI: 10.1292/jvms.20-0225</identifier><identifier>PMID: 32759575</identifier><language>eng</language><publisher>Tokyo: JAPANESE SOCIETY OF VETERINARY SCIENCE</publisher><subject>Actin ; autoimmune disease ; Fibroblasts ; Fibrosis ; Heart diseases ; Homeostasis ; Immunization ; Macrophages ; Myocarditis ; Pathology ; periostin ; Rodents ; Smooth muscle ; smooth muscle actin</subject><ispartof>Journal of Veterinary Medical Science, 2020, Vol.82(10), pp.1545-1550</ispartof><rights>2020 by the Japanese Society of Veterinary Science</rights><rights>2020. This work is published under https://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 The Japanese Society of Veterinary Science 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c539t-89892d434b94e746b9b95f92a4ed25d417637c00b59444c9183bd0b6da3fba233</citedby><cites>FETCH-LOGICAL-c539t-89892d434b94e746b9b95f92a4ed25d417637c00b59444c9183bd0b6da3fba233</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7653304/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7653304/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,1877,4010,27900,27901,27902,53766,53768</link.rule.ids></links><search><creatorcontrib>CHOI, Yuna</creatorcontrib><creatorcontrib>OH, Hanseul</creatorcontrib><creatorcontrib>AHN, Meejung</creatorcontrib><creatorcontrib>KANG, Taeyoung</creatorcontrib><creatorcontrib>CHUN, Jiyoon</creatorcontrib><creatorcontrib>SHIN, Taekyun</creatorcontrib><creatorcontrib>KIM, Jeongtae</creatorcontrib><title>Immunohistochemical analysis of periostin in the hearts of Lewis rats with experimental autoimmune myocarditis</title><title>Journal of Veterinary Medical Science</title><addtitle>J. Vet. Med. Sci.</addtitle><description>Periostin plays a critical role in tissue regeneration and homeostasis. The aim of this study was to evaluate the changes in periostin levels in the hearts of rats with experimental autoimmune myocarditis (EAM). Western blot analysis revealed that the expression levels of periostin and alpha-smooth muscle actin were significantly increased at day 14 post-immunization. Immunohistochemical analysis indicated that periostin was expressed in macrophages and fibroblasts in the hearts of EAM-induced rats. In conclusion, these results suggest that increased periostin expression in macrophages and fibroblasts promotes cardiac fibrosis in EAM-induced rats, potentially by enhancing immune cell infiltration. Therefore, periostin should be further investigated as a candidate therapeutic target for myocarditis.</description><subject>Actin</subject><subject>autoimmune disease</subject><subject>Fibroblasts</subject><subject>Fibrosis</subject><subject>Heart diseases</subject><subject>Homeostasis</subject><subject>Immunization</subject><subject>Macrophages</subject><subject>Myocarditis</subject><subject>Pathology</subject><subject>periostin</subject><subject>Rodents</subject><subject>Smooth muscle</subject><subject>smooth muscle actin</subject><issn>0916-7250</issn><issn>1347-7439</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNpdkU9r3DAQxUVpaTZpb_0Ahlx6qFP9taxLICxNGljopT0LWR7HWmxrI8lJ9ttHzi4LDQiJ4f3mMZqH0DeCrwhV9Of2aYxXFJeYUvEBrQjjspScqY9ohRWpSkkFPkPnMW4xpoRX6jM6Y1QKJaRYoel-HOfJ9y4mb3sYnTVDYSYz7KOLhe-KHQTnY3JTkU_qoejBhPQmbeA5M8Hk6tmlvoCXBR5hSovHnLxbvKEY996a0Lrk4hf0qTNDhK_H9wL9u_31d_273Py5u1_fbEormEplrWpFW854ozhIXjWqUaJT1HBoqWg5kRWTFuNGKM65VaRmTYubqjWsawxl7AJdH3x3czNCa_NMwQx6l8czYa-9cfp_ZXK9fvBPWlaCMcyzwfejQfCPM8SkRxctDIOZwM9RU85IjVXFVEYv36FbP4e8woUSef35H3WmfhwoG3yMAbrTMATrJUi9BKkp1kuQGV8f8G1M5gFOcN69swMc4Jouzfk-dp1U25ugYWKvPleqwQ</recordid><startdate>2020</startdate><enddate>2020</enddate><creator>CHOI, Yuna</creator><creator>OH, Hanseul</creator><creator>AHN, Meejung</creator><creator>KANG, Taeyoung</creator><creator>CHUN, Jiyoon</creator><creator>SHIN, Taekyun</creator><creator>KIM, Jeongtae</creator><general>JAPANESE SOCIETY OF VETERINARY SCIENCE</general><general>Japan Science and Technology Agency</general><general>The Japanese Society of Veterinary Science</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>2020</creationdate><title>Immunohistochemical analysis of periostin in the hearts of Lewis rats with experimental autoimmune myocarditis</title><author>CHOI, Yuna ; OH, Hanseul ; AHN, Meejung ; KANG, Taeyoung ; CHUN, Jiyoon ; SHIN, Taekyun ; KIM, Jeongtae</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c539t-89892d434b94e746b9b95f92a4ed25d417637c00b59444c9183bd0b6da3fba233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Actin</topic><topic>autoimmune disease</topic><topic>Fibroblasts</topic><topic>Fibrosis</topic><topic>Heart diseases</topic><topic>Homeostasis</topic><topic>Immunization</topic><topic>Macrophages</topic><topic>Myocarditis</topic><topic>Pathology</topic><topic>periostin</topic><topic>Rodents</topic><topic>Smooth muscle</topic><topic>smooth muscle actin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>CHOI, Yuna</creatorcontrib><creatorcontrib>OH, Hanseul</creatorcontrib><creatorcontrib>AHN, Meejung</creatorcontrib><creatorcontrib>KANG, Taeyoung</creatorcontrib><creatorcontrib>CHUN, Jiyoon</creatorcontrib><creatorcontrib>SHIN, Taekyun</creatorcontrib><creatorcontrib>KIM, Jeongtae</creatorcontrib><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Veterinary Medical Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>CHOI, Yuna</au><au>OH, Hanseul</au><au>AHN, Meejung</au><au>KANG, Taeyoung</au><au>CHUN, Jiyoon</au><au>SHIN, Taekyun</au><au>KIM, Jeongtae</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunohistochemical analysis of periostin in the hearts of Lewis rats with experimental autoimmune myocarditis</atitle><jtitle>Journal of Veterinary Medical Science</jtitle><addtitle>J. Vet. Med. Sci.</addtitle><date>2020</date><risdate>2020</risdate><volume>82</volume><issue>10</issue><spage>1545</spage><epage>1550</epage><pages>1545-1550</pages><issn>0916-7250</issn><eissn>1347-7439</eissn><abstract>Periostin plays a critical role in tissue regeneration and homeostasis. The aim of this study was to evaluate the changes in periostin levels in the hearts of rats with experimental autoimmune myocarditis (EAM). Western blot analysis revealed that the expression levels of periostin and alpha-smooth muscle actin were significantly increased at day 14 post-immunization. Immunohistochemical analysis indicated that periostin was expressed in macrophages and fibroblasts in the hearts of EAM-induced rats. In conclusion, these results suggest that increased periostin expression in macrophages and fibroblasts promotes cardiac fibrosis in EAM-induced rats, potentially by enhancing immune cell infiltration. 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| source | J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access |
| subjects | Actin autoimmune disease Fibroblasts Fibrosis Heart diseases Homeostasis Immunization Macrophages Myocarditis Pathology periostin Rodents Smooth muscle smooth muscle actin |
| title | Immunohistochemical analysis of periostin in the hearts of Lewis rats with experimental autoimmune myocarditis |
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