Detection and molecular characterization of kobuvirus from diarrheic goats in Minnesota
Kobuvirus infections are common among humans, rodents, carnivores, pigs, and ruminants. We report herein the complete genome sequence of a novel caprine kobuvirus (MN604700) from diarrheic kids in Minnesota. Whole-genome sequencing revealed a kobuvirus genome of 8,139 nt with a single ORF region enc...
Gespeichert in:
| Veröffentlicht in: | Journal of veterinary diagnostic investigation 2020-11, Vol.32 (6), p.873-879 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 879 |
|---|---|
| container_issue | 6 |
| container_start_page | 873 |
| container_title | Journal of veterinary diagnostic investigation |
| container_volume | 32 |
| creator | Sobhy, Nader M. Armién, Aníbal G. Wünschmann, Arno Muldoon, Dean Goyal, Sagar M. Mor, Sunil K. |
| description | Kobuvirus infections are common among humans, rodents, carnivores, pigs, and ruminants. We report herein the complete genome sequence of a novel caprine kobuvirus (MN604700) from diarrheic kids in Minnesota. Whole-genome sequencing revealed a kobuvirus genome of 8,139 nt with a single ORF region encoding a polyprotein of 2,480 amino acids. Further analysis revealed nt substitutions along the genome compared with that of the caprine kobuvirus reference strain, with 93% identity. Phylogenetic analysis indicated that the clade of the caprine kobuvirus was most closely related to porcine kobuviruses rather than bovine or ovine kobuviruses. Using primers designed from this genome, caprine kobuvirus was identified in the stools of other goats. Sanger sequencing of PCR products indicated 3D and VP1 gene nucleotides of this latter strain were 95% and 91% identical with those of MN604700, respectively. There were 35 and 101 nt substitutions in 3D and VP1 genes, respectively. Findings of kobuvirus over a 2-y period may indicate an endemic state, which needs further research. In addition, screening for kobuviruses over large geographic areas is needed to identify the evolutionary connections among different strains. |
| doi_str_mv | 10.1177/1040638720949475 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7649533</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sage_id>10.1177_1040638720949475</sage_id><sourcerecordid>2457277268</sourcerecordid><originalsourceid>FETCH-LOGICAL-c434t-ef5385e5adf5f360e7a10836b14bb9fe646037de9a83563001ff8daffc25ce683</originalsourceid><addsrcrecordid>eNp1UUtv1DAQthAVXRbunJCPXAJ2_EouSKjlJW3VSxFHa-KMd10Se7GTSvDrm3ZL1VbiNCN9r9F8hLzh7D3nxnzgTDItGlOzVrbSqGdkxVspKtkK_XzZF7i6wY_Jy1IuGVO1MvwFORaCS2ZYuyI_T3FCN4UUKcSejmlANw-QqdtBBjdhDn_hFk6e_krdfBXyXKjPaaR9gJx3GBzdJpgKDZGehRixpAlekSMPQ8HXd3NNfnz5fHHyrdqcf_1-8mlTOSnkVKFXolGooPfKC83QAGeN0B2XXdd61FIzYXpsoRFKC8a4900P3rtaOdSNWJOPB9_93I3YO4xThsHucxgh_7EJgn2MxLCz23RljZatEmIxeHdnkNPvGctkx1AcDgNETHOxtVSmNqa-zWIHqsuplIz-PoYze9OHfdrHInn78Lx7wb8CFkJ1IBTYor1Mc47Lu_5veA1A1JUc</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2457277268</pqid></control><display><type>article</type><title>Detection and molecular characterization of kobuvirus from diarrheic goats in Minnesota</title><source>SAGE Complete A-Z List</source><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Sobhy, Nader M. ; Armién, Aníbal G. ; Wünschmann, Arno ; Muldoon, Dean ; Goyal, Sagar M. ; Mor, Sunil K.</creator><creatorcontrib>Sobhy, Nader M. ; Armién, Aníbal G. ; Wünschmann, Arno ; Muldoon, Dean ; Goyal, Sagar M. ; Mor, Sunil K.</creatorcontrib><description>Kobuvirus infections are common among humans, rodents, carnivores, pigs, and ruminants. We report herein the complete genome sequence of a novel caprine kobuvirus (MN604700) from diarrheic kids in Minnesota. Whole-genome sequencing revealed a kobuvirus genome of 8,139 nt with a single ORF region encoding a polyprotein of 2,480 amino acids. Further analysis revealed nt substitutions along the genome compared with that of the caprine kobuvirus reference strain, with 93% identity. Phylogenetic analysis indicated that the clade of the caprine kobuvirus was most closely related to porcine kobuviruses rather than bovine or ovine kobuviruses. Using primers designed from this genome, caprine kobuvirus was identified in the stools of other goats. Sanger sequencing of PCR products indicated 3D and VP1 gene nucleotides of this latter strain were 95% and 91% identical with those of MN604700, respectively. There were 35 and 101 nt substitutions in 3D and VP1 genes, respectively. Findings of kobuvirus over a 2-y period may indicate an endemic state, which needs further research. In addition, screening for kobuviruses over large geographic areas is needed to identify the evolutionary connections among different strains.</description><identifier>ISSN: 1040-6387</identifier><identifier>EISSN: 1943-4936</identifier><identifier>DOI: 10.1177/1040638720949475</identifier><identifier>PMID: 33140709</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>Animals ; Diarrhea - epidemiology ; Diarrhea - veterinary ; Diarrhea - virology ; DNA Primers ; Feces - virology ; Full Scientific Reports ; Goat Diseases - epidemiology ; Goat Diseases - virology ; Goats ; Kobuvirus - genetics ; Kobuvirus - isolation & purification ; Minnesota - epidemiology ; Phylogeny ; Picornaviridae Infections - epidemiology ; Picornaviridae Infections - veterinary ; Picornaviridae Infections - virology</subject><ispartof>Journal of veterinary diagnostic investigation, 2020-11, Vol.32 (6), p.873-879</ispartof><rights>2020 The Author(s)</rights><rights>2020 The Author(s) 2020 American Association of Veterinary Laboratory Diagnosticians</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c434t-ef5385e5adf5f360e7a10836b14bb9fe646037de9a83563001ff8daffc25ce683</citedby><cites>FETCH-LOGICAL-c434t-ef5385e5adf5f360e7a10836b14bb9fe646037de9a83563001ff8daffc25ce683</cites><orcidid>0000-0003-4292-4896 ; 0000-0002-9834-9627</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7649533/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7649533/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,21800,27903,27904,43600,43601,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33140709$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sobhy, Nader M.</creatorcontrib><creatorcontrib>Armién, Aníbal G.</creatorcontrib><creatorcontrib>Wünschmann, Arno</creatorcontrib><creatorcontrib>Muldoon, Dean</creatorcontrib><creatorcontrib>Goyal, Sagar M.</creatorcontrib><creatorcontrib>Mor, Sunil K.</creatorcontrib><title>Detection and molecular characterization of kobuvirus from diarrheic goats in Minnesota</title><title>Journal of veterinary diagnostic investigation</title><addtitle>J Vet Diagn Invest</addtitle><description>Kobuvirus infections are common among humans, rodents, carnivores, pigs, and ruminants. We report herein the complete genome sequence of a novel caprine kobuvirus (MN604700) from diarrheic kids in Minnesota. Whole-genome sequencing revealed a kobuvirus genome of 8,139 nt with a single ORF region encoding a polyprotein of 2,480 amino acids. Further analysis revealed nt substitutions along the genome compared with that of the caprine kobuvirus reference strain, with 93% identity. Phylogenetic analysis indicated that the clade of the caprine kobuvirus was most closely related to porcine kobuviruses rather than bovine or ovine kobuviruses. Using primers designed from this genome, caprine kobuvirus was identified in the stools of other goats. Sanger sequencing of PCR products indicated 3D and VP1 gene nucleotides of this latter strain were 95% and 91% identical with those of MN604700, respectively. There were 35 and 101 nt substitutions in 3D and VP1 genes, respectively. Findings of kobuvirus over a 2-y period may indicate an endemic state, which needs further research. In addition, screening for kobuviruses over large geographic areas is needed to identify the evolutionary connections among different strains.</description><subject>Animals</subject><subject>Diarrhea - epidemiology</subject><subject>Diarrhea - veterinary</subject><subject>Diarrhea - virology</subject><subject>DNA Primers</subject><subject>Feces - virology</subject><subject>Full Scientific Reports</subject><subject>Goat Diseases - epidemiology</subject><subject>Goat Diseases - virology</subject><subject>Goats</subject><subject>Kobuvirus - genetics</subject><subject>Kobuvirus - isolation & purification</subject><subject>Minnesota - epidemiology</subject><subject>Phylogeny</subject><subject>Picornaviridae Infections - epidemiology</subject><subject>Picornaviridae Infections - veterinary</subject><subject>Picornaviridae Infections - virology</subject><issn>1040-6387</issn><issn>1943-4936</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1UUtv1DAQthAVXRbunJCPXAJ2_EouSKjlJW3VSxFHa-KMd10Se7GTSvDrm3ZL1VbiNCN9r9F8hLzh7D3nxnzgTDItGlOzVrbSqGdkxVspKtkK_XzZF7i6wY_Jy1IuGVO1MvwFORaCS2ZYuyI_T3FCN4UUKcSejmlANw-QqdtBBjdhDn_hFk6e_krdfBXyXKjPaaR9gJx3GBzdJpgKDZGehRixpAlekSMPQ8HXd3NNfnz5fHHyrdqcf_1-8mlTOSnkVKFXolGooPfKC83QAGeN0B2XXdd61FIzYXpsoRFKC8a4900P3rtaOdSNWJOPB9_93I3YO4xThsHucxgh_7EJgn2MxLCz23RljZatEmIxeHdnkNPvGctkx1AcDgNETHOxtVSmNqa-zWIHqsuplIz-PoYze9OHfdrHInn78Lx7wb8CFkJ1IBTYor1Mc47Lu_5veA1A1JUc</recordid><startdate>20201101</startdate><enddate>20201101</enddate><creator>Sobhy, Nader M.</creator><creator>Armién, Aníbal G.</creator><creator>Wünschmann, Arno</creator><creator>Muldoon, Dean</creator><creator>Goyal, Sagar M.</creator><creator>Mor, Sunil K.</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-4292-4896</orcidid><orcidid>https://orcid.org/0000-0002-9834-9627</orcidid></search><sort><creationdate>20201101</creationdate><title>Detection and molecular characterization of kobuvirus from diarrheic goats in Minnesota</title><author>Sobhy, Nader M. ; Armién, Aníbal G. ; Wünschmann, Arno ; Muldoon, Dean ; Goyal, Sagar M. ; Mor, Sunil K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c434t-ef5385e5adf5f360e7a10836b14bb9fe646037de9a83563001ff8daffc25ce683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Diarrhea - epidemiology</topic><topic>Diarrhea - veterinary</topic><topic>Diarrhea - virology</topic><topic>DNA Primers</topic><topic>Feces - virology</topic><topic>Full Scientific Reports</topic><topic>Goat Diseases - epidemiology</topic><topic>Goat Diseases - virology</topic><topic>Goats</topic><topic>Kobuvirus - genetics</topic><topic>Kobuvirus - isolation & purification</topic><topic>Minnesota - epidemiology</topic><topic>Phylogeny</topic><topic>Picornaviridae Infections - epidemiology</topic><topic>Picornaviridae Infections - veterinary</topic><topic>Picornaviridae Infections - virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sobhy, Nader M.</creatorcontrib><creatorcontrib>Armién, Aníbal G.</creatorcontrib><creatorcontrib>Wünschmann, Arno</creatorcontrib><creatorcontrib>Muldoon, Dean</creatorcontrib><creatorcontrib>Goyal, Sagar M.</creatorcontrib><creatorcontrib>Mor, Sunil K.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of veterinary diagnostic investigation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sobhy, Nader M.</au><au>Armién, Aníbal G.</au><au>Wünschmann, Arno</au><au>Muldoon, Dean</au><au>Goyal, Sagar M.</au><au>Mor, Sunil K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection and molecular characterization of kobuvirus from diarrheic goats in Minnesota</atitle><jtitle>Journal of veterinary diagnostic investigation</jtitle><addtitle>J Vet Diagn Invest</addtitle><date>2020-11-01</date><risdate>2020</risdate><volume>32</volume><issue>6</issue><spage>873</spage><epage>879</epage><pages>873-879</pages><issn>1040-6387</issn><eissn>1943-4936</eissn><abstract>Kobuvirus infections are common among humans, rodents, carnivores, pigs, and ruminants. We report herein the complete genome sequence of a novel caprine kobuvirus (MN604700) from diarrheic kids in Minnesota. Whole-genome sequencing revealed a kobuvirus genome of 8,139 nt with a single ORF region encoding a polyprotein of 2,480 amino acids. Further analysis revealed nt substitutions along the genome compared with that of the caprine kobuvirus reference strain, with 93% identity. Phylogenetic analysis indicated that the clade of the caprine kobuvirus was most closely related to porcine kobuviruses rather than bovine or ovine kobuviruses. Using primers designed from this genome, caprine kobuvirus was identified in the stools of other goats. Sanger sequencing of PCR products indicated 3D and VP1 gene nucleotides of this latter strain were 95% and 91% identical with those of MN604700, respectively. There were 35 and 101 nt substitutions in 3D and VP1 genes, respectively. Findings of kobuvirus over a 2-y period may indicate an endemic state, which needs further research. In addition, screening for kobuviruses over large geographic areas is needed to identify the evolutionary connections among different strains.</abstract><cop>Los Angeles, CA</cop><pub>SAGE Publications</pub><pmid>33140709</pmid><doi>10.1177/1040638720949475</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0003-4292-4896</orcidid><orcidid>https://orcid.org/0000-0002-9834-9627</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1040-6387 |
| ispartof | Journal of veterinary diagnostic investigation, 2020-11, Vol.32 (6), p.873-879 |
| issn | 1040-6387 1943-4936 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7649533 |
| source | SAGE Complete A-Z List; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Animals Diarrhea - epidemiology Diarrhea - veterinary Diarrhea - virology DNA Primers Feces - virology Full Scientific Reports Goat Diseases - epidemiology Goat Diseases - virology Goats Kobuvirus - genetics Kobuvirus - isolation & purification Minnesota - epidemiology Phylogeny Picornaviridae Infections - epidemiology Picornaviridae Infections - veterinary Picornaviridae Infections - virology |
| title | Detection and molecular characterization of kobuvirus from diarrheic goats in Minnesota |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T19%3A11%3A23IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Detection%20and%20molecular%20characterization%20of%20kobuvirus%20from%20diarrheic%20goats%20in%20Minnesota&rft.jtitle=Journal%20of%20veterinary%20diagnostic%20investigation&rft.au=Sobhy,%20Nader%20M.&rft.date=2020-11-01&rft.volume=32&rft.issue=6&rft.spage=873&rft.epage=879&rft.pages=873-879&rft.issn=1040-6387&rft.eissn=1943-4936&rft_id=info:doi/10.1177/1040638720949475&rft_dat=%3Cproquest_pubme%3E2457277268%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2457277268&rft_id=info:pmid/33140709&rft_sage_id=10.1177_1040638720949475&rfr_iscdi=true |