Case Report: Confirmation by Metagenomic Sequencing of Visceral Leishmaniasis in an Immunosuppressed Returned Traveler
There has been increased interest in using metagenomic next-generation sequencing as an unbiased approach for diagnosing infectious diseases. We describe a 61-year-old man on fingolimod therapy for multiple sclerosis with an extensive travel history who presented with 7 months of fevers, night sweat...
Gespeichert in:
Veröffentlicht in: | The American journal of tropical medicine and hygiene 2020-11, Vol.103 (5), p.1930-1933 |
---|---|
Hauptverfasser: | , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 1933 |
---|---|
container_issue | 5 |
container_start_page | 1930 |
container_title | The American journal of tropical medicine and hygiene |
container_volume | 103 |
creator | Williams, Eloise Isles, Nicole S Seemann, Torsten Kilpatrick, Trevor Grigg, Andrew Leroi, Marcel Howden, Benjamin P Kwong, Jason C |
description | There has been increased interest in using metagenomic next-generation sequencing as an unbiased approach for diagnosing infectious diseases. We describe a 61-year-old man on fingolimod therapy for multiple sclerosis with an extensive travel history who presented with 7 months of fevers, night sweats, and weight loss. Peripheral blood tests showed pancytopenia and abnormal acute phase reactants. A bone marrow aspirate showed the presence of numerous intracellular and extracellular amastigotes consistent with visceral leishmaniasis (VL). Metagenomic sequencing of the bone marrow aspirate confirmed
, a species widely reported in the Mediterranean region. This correlated with acquisition of VL infection during the patient's most recent epidemiological exposure in southern Italy 12 months prior. This case demonstrates the potential application of metagenomic sequencing for identification and speciation of
in cases of VL; however, further assessment is required using other more readily obtained clinical samples such as blood. |
doi_str_mv | 10.4269/ajtmh.19-0841 |
format | Article |
fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7646797</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2444876556</sourcerecordid><originalsourceid>FETCH-LOGICAL-c415t-509dd3e7b8977932d3e408f39ecc78c6933d128da398691e375b165e90aaad1d3</originalsourceid><addsrcrecordid>eNpdkU2LFDEQhoMo7uzq0asEvHjpNel8dTwIMri6MCLo6jVk0tUzGTpJm3QP7L83466LeqqCeniphxehF5Rc8lbqN_Ywh_0l1Q3pOH2EVpQr2VDJxWO0IoS0jZZMnaHzUg6E0K6l9Ck6Y60WWgm9Qse1LYC_wpTy_BavUxx8Dnb2KeLtLf4Ms91BTME7_A1-LhCdjzucBvzDFwfZjngDvuyDjd4WX7CP2EZ8HcISU1mmKUMp0Nf8ecmxLjfZHmGE_Aw9GexY4Pn9vEDfrz7crD81my8fr9fvN43jVMyNILrvGahtp5XSrK07J93ANDinOic1Yz1tu94y3UlNgSmxpVKAJtbanvbsAr27y52WbYDeQZzr02bKPth8a5L15t9L9HuzS0ejJJdKqxrw-j4gp-pfZhNO5uNoI6SlmJZz3ikphKzoq__QQ6rWVa9SijMiZUcq1dxRLqdSMgwPz1BiTo2a340aqs2p0cq__Nvggf5TIfsFEDOfpw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2474306680</pqid></control><display><type>article</type><title>Case Report: Confirmation by Metagenomic Sequencing of Visceral Leishmaniasis in an Immunosuppressed Returned Traveler</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Williams, Eloise ; Isles, Nicole S ; Seemann, Torsten ; Kilpatrick, Trevor ; Grigg, Andrew ; Leroi, Marcel ; Howden, Benjamin P ; Kwong, Jason C</creator><creatorcontrib>Williams, Eloise ; Isles, Nicole S ; Seemann, Torsten ; Kilpatrick, Trevor ; Grigg, Andrew ; Leroi, Marcel ; Howden, Benjamin P ; Kwong, Jason C</creatorcontrib><description>There has been increased interest in using metagenomic next-generation sequencing as an unbiased approach for diagnosing infectious diseases. We describe a 61-year-old man on fingolimod therapy for multiple sclerosis with an extensive travel history who presented with 7 months of fevers, night sweats, and weight loss. Peripheral blood tests showed pancytopenia and abnormal acute phase reactants. A bone marrow aspirate showed the presence of numerous intracellular and extracellular amastigotes consistent with visceral leishmaniasis (VL). Metagenomic sequencing of the bone marrow aspirate confirmed
, a species widely reported in the Mediterranean region. This correlated with acquisition of VL infection during the patient's most recent epidemiological exposure in southern Italy 12 months prior. This case demonstrates the potential application of metagenomic sequencing for identification and speciation of
in cases of VL; however, further assessment is required using other more readily obtained clinical samples such as blood.</description><identifier>ISSN: 0002-9637</identifier><identifier>EISSN: 1476-1645</identifier><identifier>DOI: 10.4269/ajtmh.19-0841</identifier><identifier>PMID: 32959759</identifier><language>eng</language><publisher>United States: Institute of Tropical Medicine</publisher><subject>Bone marrow ; Bone Marrow - parasitology ; Case reports ; Humans ; Immunocompromised Host ; Italy ; Leishmania infantum - genetics ; Leishmania infantum - isolation & purification ; Leishmaniasis, Visceral - diagnosis ; Leishmaniasis, Visceral - parasitology ; Male ; Metagenomics ; Middle Aged ; Parasitic diseases ; Travel</subject><ispartof>The American journal of tropical medicine and hygiene, 2020-11, Vol.103 (5), p.1930-1933</ispartof><rights>Copyright Institute of Tropical Medicine Nov 2020</rights><rights>The American Society of Tropical Medicine and Hygiene 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-509dd3e7b8977932d3e408f39ecc78c6933d128da398691e375b165e90aaad1d3</citedby><cites>FETCH-LOGICAL-c415t-509dd3e7b8977932d3e408f39ecc78c6933d128da398691e375b165e90aaad1d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7646797/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7646797/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32959759$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Williams, Eloise</creatorcontrib><creatorcontrib>Isles, Nicole S</creatorcontrib><creatorcontrib>Seemann, Torsten</creatorcontrib><creatorcontrib>Kilpatrick, Trevor</creatorcontrib><creatorcontrib>Grigg, Andrew</creatorcontrib><creatorcontrib>Leroi, Marcel</creatorcontrib><creatorcontrib>Howden, Benjamin P</creatorcontrib><creatorcontrib>Kwong, Jason C</creatorcontrib><title>Case Report: Confirmation by Metagenomic Sequencing of Visceral Leishmaniasis in an Immunosuppressed Returned Traveler</title><title>The American journal of tropical medicine and hygiene</title><addtitle>Am J Trop Med Hyg</addtitle><description>There has been increased interest in using metagenomic next-generation sequencing as an unbiased approach for diagnosing infectious diseases. We describe a 61-year-old man on fingolimod therapy for multiple sclerosis with an extensive travel history who presented with 7 months of fevers, night sweats, and weight loss. Peripheral blood tests showed pancytopenia and abnormal acute phase reactants. A bone marrow aspirate showed the presence of numerous intracellular and extracellular amastigotes consistent with visceral leishmaniasis (VL). Metagenomic sequencing of the bone marrow aspirate confirmed
, a species widely reported in the Mediterranean region. This correlated with acquisition of VL infection during the patient's most recent epidemiological exposure in southern Italy 12 months prior. This case demonstrates the potential application of metagenomic sequencing for identification and speciation of
in cases of VL; however, further assessment is required using other more readily obtained clinical samples such as blood.</description><subject>Bone marrow</subject><subject>Bone Marrow - parasitology</subject><subject>Case reports</subject><subject>Humans</subject><subject>Immunocompromised Host</subject><subject>Italy</subject><subject>Leishmania infantum - genetics</subject><subject>Leishmania infantum - isolation & purification</subject><subject>Leishmaniasis, Visceral - diagnosis</subject><subject>Leishmaniasis, Visceral - parasitology</subject><subject>Male</subject><subject>Metagenomics</subject><subject>Middle Aged</subject><subject>Parasitic diseases</subject><subject>Travel</subject><issn>0002-9637</issn><issn>1476-1645</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkU2LFDEQhoMo7uzq0asEvHjpNel8dTwIMri6MCLo6jVk0tUzGTpJm3QP7L83466LeqqCeniphxehF5Rc8lbqN_Ywh_0l1Q3pOH2EVpQr2VDJxWO0IoS0jZZMnaHzUg6E0K6l9Ck6Y60WWgm9Qse1LYC_wpTy_BavUxx8Dnb2KeLtLf4Ms91BTME7_A1-LhCdjzucBvzDFwfZjngDvuyDjd4WX7CP2EZ8HcISU1mmKUMp0Nf8ecmxLjfZHmGE_Aw9GexY4Pn9vEDfrz7crD81my8fr9fvN43jVMyNILrvGahtp5XSrK07J93ANDinOic1Yz1tu94y3UlNgSmxpVKAJtbanvbsAr27y52WbYDeQZzr02bKPth8a5L15t9L9HuzS0ejJJdKqxrw-j4gp-pfZhNO5uNoI6SlmJZz3ikphKzoq__QQ6rWVa9SijMiZUcq1dxRLqdSMgwPz1BiTo2a340aqs2p0cq__Nvggf5TIfsFEDOfpw</recordid><startdate>20201101</startdate><enddate>20201101</enddate><creator>Williams, Eloise</creator><creator>Isles, Nicole S</creator><creator>Seemann, Torsten</creator><creator>Kilpatrick, Trevor</creator><creator>Grigg, Andrew</creator><creator>Leroi, Marcel</creator><creator>Howden, Benjamin P</creator><creator>Kwong, Jason C</creator><general>Institute of Tropical Medicine</general><general>The American Society of Tropical Medicine and Hygiene</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20201101</creationdate><title>Case Report: Confirmation by Metagenomic Sequencing of Visceral Leishmaniasis in an Immunosuppressed Returned Traveler</title><author>Williams, Eloise ; Isles, Nicole S ; Seemann, Torsten ; Kilpatrick, Trevor ; Grigg, Andrew ; Leroi, Marcel ; Howden, Benjamin P ; Kwong, Jason C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-509dd3e7b8977932d3e408f39ecc78c6933d128da398691e375b165e90aaad1d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Bone marrow</topic><topic>Bone Marrow - parasitology</topic><topic>Case reports</topic><topic>Humans</topic><topic>Immunocompromised Host</topic><topic>Italy</topic><topic>Leishmania infantum - genetics</topic><topic>Leishmania infantum - isolation & purification</topic><topic>Leishmaniasis, Visceral - diagnosis</topic><topic>Leishmaniasis, Visceral - parasitology</topic><topic>Male</topic><topic>Metagenomics</topic><topic>Middle Aged</topic><topic>Parasitic diseases</topic><topic>Travel</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Williams, Eloise</creatorcontrib><creatorcontrib>Isles, Nicole S</creatorcontrib><creatorcontrib>Seemann, Torsten</creatorcontrib><creatorcontrib>Kilpatrick, Trevor</creatorcontrib><creatorcontrib>Grigg, Andrew</creatorcontrib><creatorcontrib>Leroi, Marcel</creatorcontrib><creatorcontrib>Howden, Benjamin P</creatorcontrib><creatorcontrib>Kwong, Jason C</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The American journal of tropical medicine and hygiene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Williams, Eloise</au><au>Isles, Nicole S</au><au>Seemann, Torsten</au><au>Kilpatrick, Trevor</au><au>Grigg, Andrew</au><au>Leroi, Marcel</au><au>Howden, Benjamin P</au><au>Kwong, Jason C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Case Report: Confirmation by Metagenomic Sequencing of Visceral Leishmaniasis in an Immunosuppressed Returned Traveler</atitle><jtitle>The American journal of tropical medicine and hygiene</jtitle><addtitle>Am J Trop Med Hyg</addtitle><date>2020-11-01</date><risdate>2020</risdate><volume>103</volume><issue>5</issue><spage>1930</spage><epage>1933</epage><pages>1930-1933</pages><issn>0002-9637</issn><eissn>1476-1645</eissn><abstract>There has been increased interest in using metagenomic next-generation sequencing as an unbiased approach for diagnosing infectious diseases. We describe a 61-year-old man on fingolimod therapy for multiple sclerosis with an extensive travel history who presented with 7 months of fevers, night sweats, and weight loss. Peripheral blood tests showed pancytopenia and abnormal acute phase reactants. A bone marrow aspirate showed the presence of numerous intracellular and extracellular amastigotes consistent with visceral leishmaniasis (VL). Metagenomic sequencing of the bone marrow aspirate confirmed
, a species widely reported in the Mediterranean region. This correlated with acquisition of VL infection during the patient's most recent epidemiological exposure in southern Italy 12 months prior. This case demonstrates the potential application of metagenomic sequencing for identification and speciation of
in cases of VL; however, further assessment is required using other more readily obtained clinical samples such as blood.</abstract><cop>United States</cop><pub>Institute of Tropical Medicine</pub><pmid>32959759</pmid><doi>10.4269/ajtmh.19-0841</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0002-9637 |
ispartof | The American journal of tropical medicine and hygiene, 2020-11, Vol.103 (5), p.1930-1933 |
issn | 0002-9637 1476-1645 |
language | eng |
recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7646797 |
source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
subjects | Bone marrow Bone Marrow - parasitology Case reports Humans Immunocompromised Host Italy Leishmania infantum - genetics Leishmania infantum - isolation & purification Leishmaniasis, Visceral - diagnosis Leishmaniasis, Visceral - parasitology Male Metagenomics Middle Aged Parasitic diseases Travel |
title | Case Report: Confirmation by Metagenomic Sequencing of Visceral Leishmaniasis in an Immunosuppressed Returned Traveler |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T06%3A45%3A23IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Case%20Report:%20Confirmation%20by%20Metagenomic%20Sequencing%20of%20Visceral%20Leishmaniasis%20in%20an%20Immunosuppressed%20Returned%20Traveler&rft.jtitle=The%20American%20journal%20of%20tropical%20medicine%20and%20hygiene&rft.au=Williams,%20Eloise&rft.date=2020-11-01&rft.volume=103&rft.issue=5&rft.spage=1930&rft.epage=1933&rft.pages=1930-1933&rft.issn=0002-9637&rft.eissn=1476-1645&rft_id=info:doi/10.4269/ajtmh.19-0841&rft_dat=%3Cproquest_pubme%3E2444876556%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2474306680&rft_id=info:pmid/32959759&rfr_iscdi=true |