Downregulation of miRNA-205 Expression and Biological Mechanism in Prostate Cancer Tumorigenesis and Bone Metastasis
Background. The expression and mechanism of microRNA-205 (miRNA-205) in prostate cancer (PCa) and its bone metastasis remain controversial. Materials and Methods. The expression and discriminating capability of miRNA-205 were assessed by drawing a forest plot and a summarized receiver operating char...
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| description | Background. The expression and mechanism of microRNA-205 (miRNA-205) in prostate cancer (PCa) and its bone metastasis remain controversial. Materials and Methods. The expression and discriminating capability of miRNA-205 were assessed by drawing a forest plot and a summarized receiver operating characteristic (SROC) curve, using data available from 27 miRNA-array and miRNA-sequencing datasets. The miRNA-205 target genes were acquired from online prediction tools, differentially upregulated genes in PCa, and differentially expressed genes (DEGs) after miRNA-205 transfection into PCa cell lines. Functional enrichment analysis was conducted to explore the biological mechanism of miRNA-205 targets. Immunohistochemistry (IHC) was applied to verify the protein level of the hub gene. Results. The expression of miRNA-205 in the PCa group (1,461 samples) was significantly lower than that in the noncancer group (510 samples), and the downregulation of miRNA-205 showed excellent sensitivity and specificity in differentiating between the two groups. In bone metastatic PCa, the miRNA-205 level was further reduced than in nonbone metastatic PCa, and it showed a good capability in distinguishing between the two groups. In total, 153 miRNA-205 targets were screened through the three aforementioned methods. Based on the results of functional enrichment analysis, the targets of miRNA-205 were mainly enriched during chromosome segregation and phospholipid-translocating ATPase activity and in the spindle microtubule and the p53 signaling pathway. CDK1 had the highest connectivity in the PPI network analysis and was screened as one of the hub genes. A statistically significant negative correlation between miRNA-205 and CDK1 was observed. The expression of CDK1 in PCa samples was pronouncedly upregulated in terms of both the mRNA level and the protein level when compared with noncancer samples. Conclusion. miRNA-205 may play a vital role in PCa tumorigenesis and bone metastasis by targeting CDK1. |
| doi_str_mv | 10.1155/2020/6037434 |
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| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7646560</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A697018981</galeid><sourcerecordid>A697018981</sourcerecordid><originalsourceid>FETCH-LOGICAL-c499t-e83f3e7d50b00cc8f5b70dc468f6907e566c6adf66157460f99d0aa9014aa423</originalsourceid><addsrcrecordid>eNqNkUuP0zAUhS0EYkZldqxRJDZIEMaOH7E3SKUMD2l4CHVvuc5161FiFzth4N_jqKUDrPDG1vV3z30chB4T_JIQzi8b3OBLgWnLKLuHzhtKWC0II_dPb0rP0EXON7gcSQRW4iE6o5S0UtLmHI1v4m1IsJ16M_oYquiqwX_9tKwbzKurH_sEOc9xE7rqtY993Hpr-uoj2J0JPg-VD9WXFPNoRqhWJlhI1XoaYvJbCJB9PmTGACVnNIUrsUfogTN9hovjvUDrt1fr1fv6-vO7D6vldW2ZUmMNkjoKbcfxBmNrpeObFneWCemEwi1wIawwnROC8JYJ7JTqsDEKE2YMa-gCvTrI7qfNAJ2FMCbT633yg0k_dTRe__0T_E5v43fdCiZ42eoCPTsKpPhtgjzqwWcLfW8CxCnrphTFZY1krvX0H_QmTimU6QrFJWu5auQdtTU9aB9cLHXtLKqXQrWYSCVJoV4cKFsWmxO4U8sE69l2Pduuj7YX_MmfY57g3yYX4PkB2PnQmVv_n3JQGHDmjiaUMyroLxxAvaY</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2458475928</pqid></control><display><type>article</type><title>Downregulation of miRNA-205 Expression and Biological Mechanism in Prostate Cancer Tumorigenesis and Bone Metastasis</title><source>PubMed Central Free</source><source>MEDLINE</source><source>PubMed Central Open Access</source><source>Wiley Online Library Open Access</source><source>Alma/SFX Local Collection</source><creator>He, Mao-Lin ; Gu, Yong-Yao ; Huang, Zhi-Guang ; Chen, Gang ; Cheng, Ji-Wen ; Li, Sheng-Hua ; Sun, Yu ; Yan, Hai-Biao</creator><contributor>Pizzimenti, Stefania ; Stefania Pizzimenti</contributor><creatorcontrib>He, Mao-Lin ; Gu, Yong-Yao ; Huang, Zhi-Guang ; Chen, Gang ; Cheng, Ji-Wen ; Li, Sheng-Hua ; Sun, Yu ; Yan, Hai-Biao ; Pizzimenti, Stefania ; Stefania Pizzimenti</creatorcontrib><description>Background. The expression and mechanism of microRNA-205 (miRNA-205) in prostate cancer (PCa) and its bone metastasis remain controversial. Materials and Methods. The expression and discriminating capability of miRNA-205 were assessed by drawing a forest plot and a summarized receiver operating characteristic (SROC) curve, using data available from 27 miRNA-array and miRNA-sequencing datasets. The miRNA-205 target genes were acquired from online prediction tools, differentially upregulated genes in PCa, and differentially expressed genes (DEGs) after miRNA-205 transfection into PCa cell lines. Functional enrichment analysis was conducted to explore the biological mechanism of miRNA-205 targets. Immunohistochemistry (IHC) was applied to verify the protein level of the hub gene. Results. The expression of miRNA-205 in the PCa group (1,461 samples) was significantly lower than that in the noncancer group (510 samples), and the downregulation of miRNA-205 showed excellent sensitivity and specificity in differentiating between the two groups. In bone metastatic PCa, the miRNA-205 level was further reduced than in nonbone metastatic PCa, and it showed a good capability in distinguishing between the two groups. In total, 153 miRNA-205 targets were screened through the three aforementioned methods. Based on the results of functional enrichment analysis, the targets of miRNA-205 were mainly enriched during chromosome segregation and phospholipid-translocating ATPase activity and in the spindle microtubule and the p53 signaling pathway. CDK1 had the highest connectivity in the PPI network analysis and was screened as one of the hub genes. A statistically significant negative correlation between miRNA-205 and CDK1 was observed. The expression of CDK1 in PCa samples was pronouncedly upregulated in terms of both the mRNA level and the protein level when compared with noncancer samples. Conclusion. miRNA-205 may play a vital role in PCa tumorigenesis and bone metastasis by targeting CDK1.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2020/6037434</identifier><identifier>PMID: 33178832</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Adenosine triphosphatase ; Archives & records ; Bone cancer ; Bone Neoplasms - secondary ; Cancer therapies ; Carcinogenesis ; Carcinogenesis - genetics ; CDC2 Protein Kinase - metabolism ; Chromosomes ; Complications and side effects ; Correlation analysis ; Down-Regulation - genetics ; Enrichment ; Gene expression ; Gene Expression Regulation, Neoplastic ; Gene Ontology ; Gene Regulatory Networks ; Genes ; Genetic aspects ; Genomes ; Health aspects ; Humans ; Immunohistochemistry ; Kaplan-Meier Estimate ; Male ; Metastases ; Metastasis ; MicroRNA ; MicroRNAs ; MicroRNAs - genetics ; MicroRNAs - metabolism ; Middle Aged ; miRNA ; mRNA ; Network analysis ; Ontology ; p53 Protein ; Pancreatic cancer ; Patient outcomes ; Phospholipid-translocating ATPase ; Phospholipids ; Prognosis ; Prostate cancer ; Prostatic Neoplasms - diagnosis ; Prostatic Neoplasms - genetics ; Prostatic Neoplasms - pathology ; Protein expression ; Protein Interaction Maps - genetics ; Proteins ; Reproducibility of Results ; Ribonucleic acid ; Risk factors ; RNA ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; ROC Curve ; Signal transduction ; Statistical analysis ; Statistical methods ; Target acquisition ; Transfection ; Tumorigenesis</subject><ispartof>BioMed research international, 2020, Vol.2020 (2020), p.1-17</ispartof><rights>Copyright © 2020 Yu Sun et al.</rights><rights>COPYRIGHT 2020 John Wiley & Sons, Inc.</rights><rights>Copyright © 2020 Yu Sun et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2020 Yu Sun et al. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-e83f3e7d50b00cc8f5b70dc468f6907e566c6adf66157460f99d0aa9014aa423</citedby><cites>FETCH-LOGICAL-c499t-e83f3e7d50b00cc8f5b70dc468f6907e566c6adf66157460f99d0aa9014aa423</cites><orcidid>0000-0003-0709-8509 ; 0000-0002-2897-5892</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7646560/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7646560/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,4024,27923,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33178832$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Pizzimenti, Stefania</contributor><contributor>Stefania Pizzimenti</contributor><creatorcontrib>He, Mao-Lin</creatorcontrib><creatorcontrib>Gu, Yong-Yao</creatorcontrib><creatorcontrib>Huang, Zhi-Guang</creatorcontrib><creatorcontrib>Chen, Gang</creatorcontrib><creatorcontrib>Cheng, Ji-Wen</creatorcontrib><creatorcontrib>Li, Sheng-Hua</creatorcontrib><creatorcontrib>Sun, Yu</creatorcontrib><creatorcontrib>Yan, Hai-Biao</creatorcontrib><title>Downregulation of miRNA-205 Expression and Biological Mechanism in Prostate Cancer Tumorigenesis and Bone Metastasis</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>Background. The expression and mechanism of microRNA-205 (miRNA-205) in prostate cancer (PCa) and its bone metastasis remain controversial. Materials and Methods. The expression and discriminating capability of miRNA-205 were assessed by drawing a forest plot and a summarized receiver operating characteristic (SROC) curve, using data available from 27 miRNA-array and miRNA-sequencing datasets. The miRNA-205 target genes were acquired from online prediction tools, differentially upregulated genes in PCa, and differentially expressed genes (DEGs) after miRNA-205 transfection into PCa cell lines. Functional enrichment analysis was conducted to explore the biological mechanism of miRNA-205 targets. Immunohistochemistry (IHC) was applied to verify the protein level of the hub gene. Results. The expression of miRNA-205 in the PCa group (1,461 samples) was significantly lower than that in the noncancer group (510 samples), and the downregulation of miRNA-205 showed excellent sensitivity and specificity in differentiating between the two groups. In bone metastatic PCa, the miRNA-205 level was further reduced than in nonbone metastatic PCa, and it showed a good capability in distinguishing between the two groups. In total, 153 miRNA-205 targets were screened through the three aforementioned methods. Based on the results of functional enrichment analysis, the targets of miRNA-205 were mainly enriched during chromosome segregation and phospholipid-translocating ATPase activity and in the spindle microtubule and the p53 signaling pathway. CDK1 had the highest connectivity in the PPI network analysis and was screened as one of the hub genes. A statistically significant negative correlation between miRNA-205 and CDK1 was observed. The expression of CDK1 in PCa samples was pronouncedly upregulated in terms of both the mRNA level and the protein level when compared with noncancer samples. Conclusion. miRNA-205 may play a vital role in PCa tumorigenesis and bone metastasis by targeting CDK1.</description><subject>Adenosine triphosphatase</subject><subject>Archives & records</subject><subject>Bone cancer</subject><subject>Bone Neoplasms - secondary</subject><subject>Cancer therapies</subject><subject>Carcinogenesis</subject><subject>Carcinogenesis - genetics</subject><subject>CDC2 Protein Kinase - metabolism</subject><subject>Chromosomes</subject><subject>Complications and side effects</subject><subject>Correlation analysis</subject><subject>Down-Regulation - genetics</subject><subject>Enrichment</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Gene Ontology</subject><subject>Gene Regulatory Networks</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Genomes</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Kaplan-Meier Estimate</subject><subject>Male</subject><subject>Metastases</subject><subject>Metastasis</subject><subject>MicroRNA</subject><subject>MicroRNAs</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>Middle Aged</subject><subject>miRNA</subject><subject>mRNA</subject><subject>Network analysis</subject><subject>Ontology</subject><subject>p53 Protein</subject><subject>Pancreatic cancer</subject><subject>Patient outcomes</subject><subject>Phospholipid-translocating ATPase</subject><subject>Phospholipids</subject><subject>Prognosis</subject><subject>Prostate cancer</subject><subject>Prostatic Neoplasms - diagnosis</subject><subject>Prostatic Neoplasms - genetics</subject><subject>Prostatic Neoplasms - pathology</subject><subject>Protein expression</subject><subject>Protein Interaction Maps - genetics</subject><subject>Proteins</subject><subject>Reproducibility of Results</subject><subject>Ribonucleic acid</subject><subject>Risk factors</subject><subject>RNA</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>ROC Curve</subject><subject>Signal transduction</subject><subject>Statistical analysis</subject><subject>Statistical methods</subject><subject>Target acquisition</subject><subject>Transfection</subject><subject>Tumorigenesis</subject><issn>2314-6133</issn><issn>2314-6141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkUuP0zAUhS0EYkZldqxRJDZIEMaOH7E3SKUMD2l4CHVvuc5161FiFzth4N_jqKUDrPDG1vV3z30chB4T_JIQzi8b3OBLgWnLKLuHzhtKWC0II_dPb0rP0EXON7gcSQRW4iE6o5S0UtLmHI1v4m1IsJ16M_oYquiqwX_9tKwbzKurH_sEOc9xE7rqtY993Hpr-uoj2J0JPg-VD9WXFPNoRqhWJlhI1XoaYvJbCJB9PmTGACVnNIUrsUfogTN9hovjvUDrt1fr1fv6-vO7D6vldW2ZUmMNkjoKbcfxBmNrpeObFneWCemEwi1wIawwnROC8JYJ7JTqsDEKE2YMa-gCvTrI7qfNAJ2FMCbT633yg0k_dTRe__0T_E5v43fdCiZ42eoCPTsKpPhtgjzqwWcLfW8CxCnrphTFZY1krvX0H_QmTimU6QrFJWu5auQdtTU9aB9cLHXtLKqXQrWYSCVJoV4cKFsWmxO4U8sE69l2Pduuj7YX_MmfY57g3yYX4PkB2PnQmVv_n3JQGHDmjiaUMyroLxxAvaY</recordid><startdate>2020</startdate><enddate>2020</enddate><creator>He, Mao-Lin</creator><creator>Gu, Yong-Yao</creator><creator>Huang, Zhi-Guang</creator><creator>Chen, Gang</creator><creator>Cheng, Ji-Wen</creator><creator>Li, Sheng-Hua</creator><creator>Sun, Yu</creator><creator>Yan, Hai-Biao</creator><general>Hindawi Publishing Corporation</general><general>Hindawi</general><general>John Wiley & Sons, Inc</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-0709-8509</orcidid><orcidid>https://orcid.org/0000-0002-2897-5892</orcidid></search><sort><creationdate>2020</creationdate><title>Downregulation of miRNA-205 Expression and Biological Mechanism in Prostate Cancer Tumorigenesis and Bone Metastasis</title><author>He, Mao-Lin ; Gu, Yong-Yao ; Huang, Zhi-Guang ; Chen, Gang ; Cheng, Ji-Wen ; Li, Sheng-Hua ; Sun, Yu ; Yan, Hai-Biao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-e83f3e7d50b00cc8f5b70dc468f6907e566c6adf66157460f99d0aa9014aa423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adenosine triphosphatase</topic><topic>Archives & records</topic><topic>Bone cancer</topic><topic>Bone Neoplasms - secondary</topic><topic>Cancer therapies</topic><topic>Carcinogenesis</topic><topic>Carcinogenesis - genetics</topic><topic>CDC2 Protein Kinase - metabolism</topic><topic>Chromosomes</topic><topic>Complications and side effects</topic><topic>Correlation analysis</topic><topic>Down-Regulation - genetics</topic><topic>Enrichment</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Gene Ontology</topic><topic>Gene Regulatory Networks</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>Genomes</topic><topic>Health aspects</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Kaplan-Meier Estimate</topic><topic>Male</topic><topic>Metastases</topic><topic>Metastasis</topic><topic>MicroRNA</topic><topic>MicroRNAs</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>Middle Aged</topic><topic>miRNA</topic><topic>mRNA</topic><topic>Network analysis</topic><topic>Ontology</topic><topic>p53 Protein</topic><topic>Pancreatic cancer</topic><topic>Patient outcomes</topic><topic>Phospholipid-translocating ATPase</topic><topic>Phospholipids</topic><topic>Prognosis</topic><topic>Prostate cancer</topic><topic>Prostatic Neoplasms - diagnosis</topic><topic>Prostatic Neoplasms - genetics</topic><topic>Prostatic Neoplasms - pathology</topic><topic>Protein expression</topic><topic>Protein Interaction Maps - genetics</topic><topic>Proteins</topic><topic>Reproducibility of Results</topic><topic>Ribonucleic acid</topic><topic>Risk factors</topic><topic>RNA</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>ROC Curve</topic><topic>Signal transduction</topic><topic>Statistical analysis</topic><topic>Statistical methods</topic><topic>Target acquisition</topic><topic>Transfection</topic><topic>Tumorigenesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>He, Mao-Lin</creatorcontrib><creatorcontrib>Gu, Yong-Yao</creatorcontrib><creatorcontrib>Huang, Zhi-Guang</creatorcontrib><creatorcontrib>Chen, Gang</creatorcontrib><creatorcontrib>Cheng, Ji-Wen</creatorcontrib><creatorcontrib>Li, Sheng-Hua</creatorcontrib><creatorcontrib>Sun, Yu</creatorcontrib><creatorcontrib>Yan, Hai-Biao</creatorcontrib><collection>الدوريات العلمية والإحصائية - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BioMed research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>He, Mao-Lin</au><au>Gu, Yong-Yao</au><au>Huang, Zhi-Guang</au><au>Chen, Gang</au><au>Cheng, Ji-Wen</au><au>Li, Sheng-Hua</au><au>Sun, Yu</au><au>Yan, Hai-Biao</au><au>Pizzimenti, Stefania</au><au>Stefania Pizzimenti</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Downregulation of miRNA-205 Expression and Biological Mechanism in Prostate Cancer Tumorigenesis and Bone Metastasis</atitle><jtitle>BioMed research international</jtitle><addtitle>Biomed Res Int</addtitle><date>2020</date><risdate>2020</risdate><volume>2020</volume><issue>2020</issue><spage>1</spage><epage>17</epage><pages>1-17</pages><issn>2314-6133</issn><eissn>2314-6141</eissn><abstract>Background. The expression and mechanism of microRNA-205 (miRNA-205) in prostate cancer (PCa) and its bone metastasis remain controversial. Materials and Methods. The expression and discriminating capability of miRNA-205 were assessed by drawing a forest plot and a summarized receiver operating characteristic (SROC) curve, using data available from 27 miRNA-array and miRNA-sequencing datasets. The miRNA-205 target genes were acquired from online prediction tools, differentially upregulated genes in PCa, and differentially expressed genes (DEGs) after miRNA-205 transfection into PCa cell lines. Functional enrichment analysis was conducted to explore the biological mechanism of miRNA-205 targets. Immunohistochemistry (IHC) was applied to verify the protein level of the hub gene. Results. The expression of miRNA-205 in the PCa group (1,461 samples) was significantly lower than that in the noncancer group (510 samples), and the downregulation of miRNA-205 showed excellent sensitivity and specificity in differentiating between the two groups. In bone metastatic PCa, the miRNA-205 level was further reduced than in nonbone metastatic PCa, and it showed a good capability in distinguishing between the two groups. In total, 153 miRNA-205 targets were screened through the three aforementioned methods. Based on the results of functional enrichment analysis, the targets of miRNA-205 were mainly enriched during chromosome segregation and phospholipid-translocating ATPase activity and in the spindle microtubule and the p53 signaling pathway. CDK1 had the highest connectivity in the PPI network analysis and was screened as one of the hub genes. A statistically significant negative correlation between miRNA-205 and CDK1 was observed. The expression of CDK1 in PCa samples was pronouncedly upregulated in terms of both the mRNA level and the protein level when compared with noncancer samples. Conclusion. miRNA-205 may play a vital role in PCa tumorigenesis and bone metastasis by targeting CDK1.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>33178832</pmid><doi>10.1155/2020/6037434</doi><tpages>17</tpages><orcidid>https://orcid.org/0000-0003-0709-8509</orcidid><orcidid>https://orcid.org/0000-0002-2897-5892</orcidid><oa>free_for_read</oa></addata></record> |
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| source | PubMed Central Free; MEDLINE; PubMed Central Open Access; Wiley Online Library Open Access; Alma/SFX Local Collection |
| subjects | Adenosine triphosphatase Archives & records Bone cancer Bone Neoplasms - secondary Cancer therapies Carcinogenesis Carcinogenesis - genetics CDC2 Protein Kinase - metabolism Chromosomes Complications and side effects Correlation analysis Down-Regulation - genetics Enrichment Gene expression Gene Expression Regulation, Neoplastic Gene Ontology Gene Regulatory Networks Genes Genetic aspects Genomes Health aspects Humans Immunohistochemistry Kaplan-Meier Estimate Male Metastases Metastasis MicroRNA MicroRNAs MicroRNAs - genetics MicroRNAs - metabolism Middle Aged miRNA mRNA Network analysis Ontology p53 Protein Pancreatic cancer Patient outcomes Phospholipid-translocating ATPase Phospholipids Prognosis Prostate cancer Prostatic Neoplasms - diagnosis Prostatic Neoplasms - genetics Prostatic Neoplasms - pathology Protein expression Protein Interaction Maps - genetics Proteins Reproducibility of Results Ribonucleic acid Risk factors RNA RNA, Messenger - genetics RNA, Messenger - metabolism ROC Curve Signal transduction Statistical analysis Statistical methods Target acquisition Transfection Tumorigenesis |
| title | Downregulation of miRNA-205 Expression and Biological Mechanism in Prostate Cancer Tumorigenesis and Bone Metastasis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-03T12%3A00%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Downregulation%20of%20miRNA-205%20Expression%20and%20Biological%20Mechanism%20in%20Prostate%20Cancer%20Tumorigenesis%20and%20Bone%20Metastasis&rft.jtitle=BioMed%20research%20international&rft.au=He,%20Mao-Lin&rft.date=2020&rft.volume=2020&rft.issue=2020&rft.spage=1&rft.epage=17&rft.pages=1-17&rft.issn=2314-6133&rft.eissn=2314-6141&rft_id=info:doi/10.1155/2020/6037434&rft_dat=%3Cgale_pubme%3EA697018981%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2458475928&rft_id=info:pmid/33178832&rft_galeid=A697018981&rfr_iscdi=true |