γ′ fibrinogen levels are associated with blood clot strength in traumatic brain injury patients
γ′ fibrinogen is an alternatively-spliced fibrinogen variant that displays different coagulation parameters in vitro than the major form of fibrinogen. Purified γ′ fibrinogen has slower clotting kinetics than unfractionated fibrinogen, but forms clots that are stronger and resistant to fibrinolysis....
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| Veröffentlicht in: | The American journal of surgery 2020-08, Vol.220 (2), p.459-463 |
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| creator | Farrell, David H. Rick, Elizabeth A. Dewey, Elizabeth N. Schreiber, Martin A. Rowell, Susan E. |
| description | γ′ fibrinogen is an alternatively-spliced fibrinogen variant that displays different coagulation parameters in vitro than the major form of fibrinogen. Purified γ′ fibrinogen has slower clotting kinetics than unfractionated fibrinogen, but forms clots that are stronger and resistant to fibrinolysis. However, these properties have only been investigated in human populations in a limited number of studies. We therefore performed a retrospective analysis to test the hypothesis that γ′ fibrinogen levels influence coagulation in vivo.
In the present study, we utilized blood samples that were collected from traumatic brain injury patients to probe the relationship between γ′ fibrinogen levels and traditional coagulation parameters.
The results show that the levels of γ′ fibrinogen were inversely associated with clotting kinetics, indicated by a shortened INR. In addition, the levels of γ′ fibrinogen were associated with stronger clots by thrombelastography. However, these changes were not associated with significant changes in hemorrhage progression.
These findings verify that γ′ fibrinogen properties observed in purified systems result in similar properties in a clinical setting, and may affect coagulation.
•The results contradict in vitrostudies that γ′fibrinogen clots slower than unfractionated fibrinogen, but confirm that it forms stronger clots.•γ′ fibrinogen levels were associated with faster clot formation in human whole blood and plasma samples.•γ′ fibrinogen levels were associated with stronger clots in human whole blood samples. |
| doi_str_mv | 10.1016/j.amjsurg.2019.12.028 |
| format | Article |
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In the present study, we utilized blood samples that were collected from traumatic brain injury patients to probe the relationship between γ′ fibrinogen levels and traditional coagulation parameters.
The results show that the levels of γ′ fibrinogen were inversely associated with clotting kinetics, indicated by a shortened INR. In addition, the levels of γ′ fibrinogen were associated with stronger clots by thrombelastography. However, these changes were not associated with significant changes in hemorrhage progression.
These findings verify that γ′ fibrinogen properties observed in purified systems result in similar properties in a clinical setting, and may affect coagulation.
•The results contradict in vitrostudies that γ′fibrinogen clots slower than unfractionated fibrinogen, but confirm that it forms stronger clots.•γ′ fibrinogen levels were associated with faster clot formation in human whole blood and plasma samples.•γ′ fibrinogen levels were associated with stronger clots in human whole blood samples.</description><identifier>ISSN: 0002-9610</identifier><identifier>EISSN: 1879-1883</identifier><identifier>DOI: 10.1016/j.amjsurg.2019.12.028</identifier><identifier>PMID: 31948701</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adolescent ; Adult ; Age ; Aged ; Aged, 80 and over ; Amino acids ; Anticoagulants ; Binding sites ; Blood ; Blood coagulation ; Blood Coagulation - physiology ; Blood platelets ; Brain ; Brain Injuries, Traumatic - complications ; Clotting ; Coagulation ; Consent ; Cytokines ; Female ; Fibrinogen ; Fibrinogen - analysis ; Fibrinolysis ; Head injuries ; Hemorrhage ; Hemostasis ; Human populations ; Humans ; Immunoassay ; In vivo methods and tests ; Kinetics ; Laboratories ; Male ; Middle Aged ; Parameters ; Patients ; Plasma ; Properties (attributes) ; Proteins ; Retrospective Studies ; Studies ; Thrombelastography ; Thrombosis ; Thrombosis - blood ; Thrombosis - etiology ; Trauma ; Traumatic brain injury ; Young Adult</subject><ispartof>The American journal of surgery, 2020-08, Vol.220 (2), p.459-463</ispartof><rights>2020 Elsevier Inc.</rights><rights>Copyright © 2020 Elsevier Inc. All rights reserved.</rights><rights>2020. Elsevier Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c495t-dd939ff42255062614bd7bbda741dd9ec6afb6f43c29954cc892d9cc98dda1133</citedby><cites>FETCH-LOGICAL-c495t-dd939ff42255062614bd7bbda741dd9ec6afb6f43c29954cc892d9cc98dda1133</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/2425699744?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995,64385,64387,64389,72469</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31948701$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Farrell, David H.</creatorcontrib><creatorcontrib>Rick, Elizabeth A.</creatorcontrib><creatorcontrib>Dewey, Elizabeth N.</creatorcontrib><creatorcontrib>Schreiber, Martin A.</creatorcontrib><creatorcontrib>Rowell, Susan E.</creatorcontrib><title>γ′ fibrinogen levels are associated with blood clot strength in traumatic brain injury patients</title><title>The American journal of surgery</title><addtitle>Am J Surg</addtitle><description>γ′ fibrinogen is an alternatively-spliced fibrinogen variant that displays different coagulation parameters in vitro than the major form of fibrinogen. Purified γ′ fibrinogen has slower clotting kinetics than unfractionated fibrinogen, but forms clots that are stronger and resistant to fibrinolysis. However, these properties have only been investigated in human populations in a limited number of studies. We therefore performed a retrospective analysis to test the hypothesis that γ′ fibrinogen levels influence coagulation in vivo.
In the present study, we utilized blood samples that were collected from traumatic brain injury patients to probe the relationship between γ′ fibrinogen levels and traditional coagulation parameters.
The results show that the levels of γ′ fibrinogen were inversely associated with clotting kinetics, indicated by a shortened INR. In addition, the levels of γ′ fibrinogen were associated with stronger clots by thrombelastography. However, these changes were not associated with significant changes in hemorrhage progression.
These findings verify that γ′ fibrinogen properties observed in purified systems result in similar properties in a clinical setting, and may affect coagulation.
•The results contradict in vitrostudies that γ′fibrinogen clots slower than unfractionated fibrinogen, but confirm that it forms stronger clots.•γ′ fibrinogen levels were associated with faster clot formation in human whole blood and plasma samples.•γ′ fibrinogen levels were associated with stronger clots in human whole blood samples.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Age</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Amino acids</subject><subject>Anticoagulants</subject><subject>Binding sites</subject><subject>Blood</subject><subject>Blood coagulation</subject><subject>Blood Coagulation - physiology</subject><subject>Blood platelets</subject><subject>Brain</subject><subject>Brain Injuries, Traumatic - complications</subject><subject>Clotting</subject><subject>Coagulation</subject><subject>Consent</subject><subject>Cytokines</subject><subject>Female</subject><subject>Fibrinogen</subject><subject>Fibrinogen - analysis</subject><subject>Fibrinolysis</subject><subject>Head injuries</subject><subject>Hemorrhage</subject><subject>Hemostasis</subject><subject>Human populations</subject><subject>Humans</subject><subject>Immunoassay</subject><subject>In vivo methods and tests</subject><subject>Kinetics</subject><subject>Laboratories</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Parameters</subject><subject>Patients</subject><subject>Plasma</subject><subject>Properties (attributes)</subject><subject>Proteins</subject><subject>Retrospective Studies</subject><subject>Studies</subject><subject>Thrombelastography</subject><subject>Thrombosis</subject><subject>Thrombosis - blood</subject><subject>Thrombosis - etiology</subject><subject>Trauma</subject><subject>Traumatic brain injury</subject><subject>Young Adult</subject><issn>0002-9610</issn><issn>1879-1883</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkUuO1DAQhi0EYpqGI4AssWGT4LKdhzcgNOIljcQG1pZf6XGUxI3tNJodZ4J7cAhOglvdjIANK6uq_vrLVR9Cj4HUQKB9PtZqHtMadzUlIGqgNaH9HbSBvhMV9D27izaEEFqJFsgFepDSWEIAzu6jCwaC9x2BDdI_vv_8-g0PXke_hJ1b8OQObkpYRYdVSsF4lZ3FX3y-xnoKwWIzhYxTjm7ZlZxfcI5qnVX2BuuoSuyXcY03eF9SbsnpIbo3qCm5R-d3iz69ef3x8l119eHt-8tXV5XhosmVtYKJYeCUNg1paQtc205rqzoOpeZMqwbdDpwZKkTDjekFtcIY0VurABjbohcn3_2qZ2dNmR3VJPfRzyreyKC8_Luy-Gu5CwfZtRx41xSDZ2eDGD6vLmU5-2TcNKnFhTVJyji0DJpywy16-o90DGtcynqSctq0QnT8qGpOKhNDStENt58BIo8U5SjPFOWRogQqC8XS9-TPTW67fmMrgpcnQSHlDt5FmUy5tXHWR2eytMH_Z8Qviy61Rw</recordid><startdate>20200801</startdate><enddate>20200801</enddate><creator>Farrell, David H.</creator><creator>Rick, Elizabeth A.</creator><creator>Dewey, Elizabeth N.</creator><creator>Schreiber, Martin A.</creator><creator>Rowell, Susan E.</creator><general>Elsevier Inc</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20200801</creationdate><title>γ′ fibrinogen levels are associated with blood clot strength in traumatic brain injury patients</title><author>Farrell, David H. ; Rick, Elizabeth A. ; Dewey, Elizabeth N. ; Schreiber, Martin A. ; Rowell, Susan E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c495t-dd939ff42255062614bd7bbda741dd9ec6afb6f43c29954cc892d9cc98dda1133</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Age</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Amino acids</topic><topic>Anticoagulants</topic><topic>Binding sites</topic><topic>Blood</topic><topic>Blood coagulation</topic><topic>Blood Coagulation - physiology</topic><topic>Blood platelets</topic><topic>Brain</topic><topic>Brain Injuries, Traumatic - complications</topic><topic>Clotting</topic><topic>Coagulation</topic><topic>Consent</topic><topic>Cytokines</topic><topic>Female</topic><topic>Fibrinogen</topic><topic>Fibrinogen - analysis</topic><topic>Fibrinolysis</topic><topic>Head injuries</topic><topic>Hemorrhage</topic><topic>Hemostasis</topic><topic>Human populations</topic><topic>Humans</topic><topic>Immunoassay</topic><topic>In vivo methods and tests</topic><topic>Kinetics</topic><topic>Laboratories</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Parameters</topic><topic>Patients</topic><topic>Plasma</topic><topic>Properties (attributes)</topic><topic>Proteins</topic><topic>Retrospective Studies</topic><topic>Studies</topic><topic>Thrombelastography</topic><topic>Thrombosis</topic><topic>Thrombosis - blood</topic><topic>Thrombosis - etiology</topic><topic>Trauma</topic><topic>Traumatic brain injury</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Farrell, David H.</creatorcontrib><creatorcontrib>Rick, Elizabeth A.</creatorcontrib><creatorcontrib>Dewey, Elizabeth N.</creatorcontrib><creatorcontrib>Schreiber, Martin A.</creatorcontrib><creatorcontrib>Rowell, Susan E.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The American journal of surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Farrell, David H.</au><au>Rick, Elizabeth A.</au><au>Dewey, Elizabeth N.</au><au>Schreiber, Martin A.</au><au>Rowell, Susan E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>γ′ fibrinogen levels are associated with blood clot strength in traumatic brain injury patients</atitle><jtitle>The American journal of surgery</jtitle><addtitle>Am J Surg</addtitle><date>2020-08-01</date><risdate>2020</risdate><volume>220</volume><issue>2</issue><spage>459</spage><epage>463</epage><pages>459-463</pages><issn>0002-9610</issn><eissn>1879-1883</eissn><abstract>γ′ fibrinogen is an alternatively-spliced fibrinogen variant that displays different coagulation parameters in vitro than the major form of fibrinogen. Purified γ′ fibrinogen has slower clotting kinetics than unfractionated fibrinogen, but forms clots that are stronger and resistant to fibrinolysis. However, these properties have only been investigated in human populations in a limited number of studies. We therefore performed a retrospective analysis to test the hypothesis that γ′ fibrinogen levels influence coagulation in vivo.
In the present study, we utilized blood samples that were collected from traumatic brain injury patients to probe the relationship between γ′ fibrinogen levels and traditional coagulation parameters.
The results show that the levels of γ′ fibrinogen were inversely associated with clotting kinetics, indicated by a shortened INR. In addition, the levels of γ′ fibrinogen were associated with stronger clots by thrombelastography. However, these changes were not associated with significant changes in hemorrhage progression.
These findings verify that γ′ fibrinogen properties observed in purified systems result in similar properties in a clinical setting, and may affect coagulation.
•The results contradict in vitrostudies that γ′fibrinogen clots slower than unfractionated fibrinogen, but confirm that it forms stronger clots.•γ′ fibrinogen levels were associated with faster clot formation in human whole blood and plasma samples.•γ′ fibrinogen levels were associated with stronger clots in human whole blood samples.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>31948701</pmid><doi>10.1016/j.amjsurg.2019.12.028</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adolescent Adult Age Aged Aged, 80 and over Amino acids Anticoagulants Binding sites Blood Blood coagulation Blood Coagulation - physiology Blood platelets Brain Brain Injuries, Traumatic - complications Clotting Coagulation Consent Cytokines Female Fibrinogen Fibrinogen - analysis Fibrinolysis Head injuries Hemorrhage Hemostasis Human populations Humans Immunoassay In vivo methods and tests Kinetics Laboratories Male Middle Aged Parameters Patients Plasma Properties (attributes) Proteins Retrospective Studies Studies Thrombelastography Thrombosis Thrombosis - blood Thrombosis - etiology Trauma Traumatic brain injury Young Adult |
| title | γ′ fibrinogen levels are associated with blood clot strength in traumatic brain injury patients |
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