Nanostring-based screening for tyrosine kinase fusions in inflammatory myofibroblastic tumors
Gene expression imbalances were measured for tyrosine kinase ( TK ) genes using Nanostring in 19 samples of inflammatory myofibroblastic tumor (IMT). All cases were immunohistochemically stained with anaplastic lymphoma kinase (ALK) and pan-tropomyosin-related-kinase (pan-Trk) antibodies. Five cases...
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| Veröffentlicht in: | Scientific reports 2020-10, Vol.10 (1), p.18724-18724, Article 18724 |
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| creator | Kurihara, Taisei Suehara, Yoshiyuki Akaike, Keisuke Hayashi, Takuo Kohsaka, Shinji Ueno, Toshihide Hasegawa, Nobuhiko Takagi, Tatsuya Sasa, Keita Okubo, Taketo Kim, Youngji Mano, Hiroyuki Yao, Takashi Kaneko, Kazuo Saito, Tsuyoshi |
| description | Gene expression imbalances were measured for tyrosine kinase (
TK
) genes using Nanostring in 19 samples of inflammatory myofibroblastic tumor (IMT). All cases were immunohistochemically stained with anaplastic lymphoma kinase (ALK) and pan-tropomyosin-related-kinase (pan-Trk) antibodies. Five cases with imbalanced
ALK
expression, reported with Nanostring, were tested using fluorescence in situ hybridization (FISH); two cases with imbalanced neurotrophic tyrosine receptor kinase 3 (
NTRK3
) expression were tested using reverse transcription-polymerase chain reaction (RT-PCR). One case with imbalanced expression for ROS proto-oncogene 1 (
ROS1
) was tested using RNA sequencing and RT-PCR. TK fusions were detected in all cases with imbalanced
TK
expression. RNA sequencing detected a
FN1–ROS1
fusion gene in an adult IMT case. IMT with
ALK
rearrangement showed myofibroblast-dominant features. IMT with
ETV6–NTRK3
fusion showed prominent lymphoplasmacytic infiltration with scattered myofibroblasts. Pan-Trk IHC revealed only scattered positively stained cells in IMT with
ETV6–NTRK3
fusion gene.
ROS1
-positive IMT showed myofibroblast-dominant features. |
| doi_str_mv | 10.1038/s41598-020-75596-3 |
| format | Article |
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TK
) genes using Nanostring in 19 samples of inflammatory myofibroblastic tumor (IMT). All cases were immunohistochemically stained with anaplastic lymphoma kinase (ALK) and pan-tropomyosin-related-kinase (pan-Trk) antibodies. Five cases with imbalanced
ALK
expression, reported with Nanostring, were tested using fluorescence in situ hybridization (FISH); two cases with imbalanced neurotrophic tyrosine receptor kinase 3 (
NTRK3
) expression were tested using reverse transcription-polymerase chain reaction (RT-PCR). One case with imbalanced expression for ROS proto-oncogene 1 (
ROS1
) was tested using RNA sequencing and RT-PCR. TK fusions were detected in all cases with imbalanced
TK
expression. RNA sequencing detected a
FN1–ROS1
fusion gene in an adult IMT case. IMT with
ALK
rearrangement showed myofibroblast-dominant features. IMT with
ETV6–NTRK3
fusion showed prominent lymphoplasmacytic infiltration with scattered myofibroblasts. Pan-Trk IHC revealed only scattered positively stained cells in IMT with
ETV6–NTRK3
fusion gene.
ROS1
-positive IMT showed myofibroblast-dominant features.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-020-75596-3</identifier><identifier>PMID: 33127954</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/208 ; 631/67 ; 692/308 ; 692/4017 ; 692/4028 ; 692/420 ; 692/699 ; Adult ; Aged ; Antibodies - chemistry ; ETS Translocation Variant 6 Protein ; Female ; Fibronectins - genetics ; Fluorescence in situ hybridization ; Fusion protein ; Gene expression ; Gene rearrangement ; Humanities and Social Sciences ; Humans ; Immunohistochemistry ; In Situ Hybridization, Fluorescence ; Inflammation ; Kinases ; Lymphoma ; Male ; Metastases ; Middle Aged ; multidisciplinary ; Myofibroblasts - enzymology ; Nanotechnology - methods ; Neoplasms - enzymology ; Polymerase chain reaction ; Protein-tyrosine kinase ; Protein-Tyrosine Kinases - genetics ; Protein-Tyrosine Kinases - metabolism ; Proto-Oncogene Mas ; Proto-Oncogene Proteins - genetics ; Proto-Oncogene Proteins c-ets - genetics ; Receptor, trkC - genetics ; Repressor Proteins - genetics ; Reverse transcription ; Ribonucleic acid ; RNA ; Science ; Science (multidisciplinary) ; Tropomyosin ; Tumors ; Young Adult</subject><ispartof>Scientific reports, 2020-10, Vol.10 (1), p.18724-18724, Article 18724</ispartof><rights>The Author(s) 2020</rights><rights>The Author(s) 2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c668t-c8b452f6ace3def1150f07cbbe48d9ecbc24084a95a255c5a1affbd0f708ee63</citedby><cites>FETCH-LOGICAL-c668t-c8b452f6ace3def1150f07cbbe48d9ecbc24084a95a255c5a1affbd0f708ee63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7603320/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7603320/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27903,27904,41099,42168,51555,53770,53772</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33127954$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kurihara, Taisei</creatorcontrib><creatorcontrib>Suehara, Yoshiyuki</creatorcontrib><creatorcontrib>Akaike, Keisuke</creatorcontrib><creatorcontrib>Hayashi, Takuo</creatorcontrib><creatorcontrib>Kohsaka, Shinji</creatorcontrib><creatorcontrib>Ueno, Toshihide</creatorcontrib><creatorcontrib>Hasegawa, Nobuhiko</creatorcontrib><creatorcontrib>Takagi, Tatsuya</creatorcontrib><creatorcontrib>Sasa, Keita</creatorcontrib><creatorcontrib>Okubo, Taketo</creatorcontrib><creatorcontrib>Kim, Youngji</creatorcontrib><creatorcontrib>Mano, Hiroyuki</creatorcontrib><creatorcontrib>Yao, Takashi</creatorcontrib><creatorcontrib>Kaneko, Kazuo</creatorcontrib><creatorcontrib>Saito, Tsuyoshi</creatorcontrib><title>Nanostring-based screening for tyrosine kinase fusions in inflammatory myofibroblastic tumors</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Gene expression imbalances were measured for tyrosine kinase (
TK
) genes using Nanostring in 19 samples of inflammatory myofibroblastic tumor (IMT). All cases were immunohistochemically stained with anaplastic lymphoma kinase (ALK) and pan-tropomyosin-related-kinase (pan-Trk) antibodies. Five cases with imbalanced
ALK
expression, reported with Nanostring, were tested using fluorescence in situ hybridization (FISH); two cases with imbalanced neurotrophic tyrosine receptor kinase 3 (
NTRK3
) expression were tested using reverse transcription-polymerase chain reaction (RT-PCR). One case with imbalanced expression for ROS proto-oncogene 1 (
ROS1
) was tested using RNA sequencing and RT-PCR. TK fusions were detected in all cases with imbalanced
TK
expression. RNA sequencing detected a
FN1–ROS1
fusion gene in an adult IMT case. IMT with
ALK
rearrangement showed myofibroblast-dominant features. IMT with
ETV6–NTRK3
fusion showed prominent lymphoplasmacytic infiltration with scattered myofibroblasts. Pan-Trk IHC revealed only scattered positively stained cells in IMT with
ETV6–NTRK3
fusion gene.
ROS1
-positive IMT showed myofibroblast-dominant features.</description><subject>631/208</subject><subject>631/67</subject><subject>692/308</subject><subject>692/4017</subject><subject>692/4028</subject><subject>692/420</subject><subject>692/699</subject><subject>Adult</subject><subject>Aged</subject><subject>Antibodies - chemistry</subject><subject>ETS Translocation Variant 6 Protein</subject><subject>Female</subject><subject>Fibronectins - genetics</subject><subject>Fluorescence in situ hybridization</subject><subject>Fusion protein</subject><subject>Gene expression</subject><subject>Gene rearrangement</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Inflammation</subject><subject>Kinases</subject><subject>Lymphoma</subject><subject>Male</subject><subject>Metastases</subject><subject>Middle Aged</subject><subject>multidisciplinary</subject><subject>Myofibroblasts - enzymology</subject><subject>Nanotechnology - methods</subject><subject>Neoplasms - enzymology</subject><subject>Polymerase chain reaction</subject><subject>Protein-tyrosine kinase</subject><subject>Protein-Tyrosine Kinases - genetics</subject><subject>Protein-Tyrosine Kinases - metabolism</subject><subject>Proto-Oncogene Mas</subject><subject>Proto-Oncogene Proteins - genetics</subject><subject>Proto-Oncogene Proteins c-ets - genetics</subject><subject>Receptor, trkC - genetics</subject><subject>Repressor Proteins - genetics</subject><subject>Reverse transcription</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Tropomyosin</subject><subject>Tumors</subject><subject>Young Adult</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kV1LHTEQhkOxVLH-gV7IgjferM3nftwIIlYFaW-8LSHJTmx0NzlmdoXz75vTY6160RDIhHnmTWZeQr4wesKo6L6iZKrvaspp3SrVN7X4QPY4larmgvOdV_EuOUC8p2Up3kvWfyK7QjDe9krukZ_fTUw45xDvamsQhgpdBojlXvmUq3mdE4YI1UOIJV35BUOKWIVYth_NNJk55XU1rZMPNic7GpyDq-ZlShk_k4_ejAgHz-c-uf12cXt-Vd_8uLw-P7upXdN0c-06KxX3jXEgBvCMKepp66wF2Q09OOu4pJ00vTJcKacMM97bgfqWdgCN2CenW9nVYicYHMQ5m1GvcphMXutkgn6bieGXvktPum2oEJwWgeNngZweF8BZTwEdjKOJkBbUXKpGctWwtqBH79D7tORYuitUyzYj5n2h-JZyZXyYwb98hlG98U9v_dPFP_3HPy1K0eHrNl5K_rpVALEFcLUxDPK_t_8j-xsRnqm4</recordid><startdate>20201030</startdate><enddate>20201030</enddate><creator>Kurihara, Taisei</creator><creator>Suehara, Yoshiyuki</creator><creator>Akaike, Keisuke</creator><creator>Hayashi, Takuo</creator><creator>Kohsaka, Shinji</creator><creator>Ueno, Toshihide</creator><creator>Hasegawa, Nobuhiko</creator><creator>Takagi, Tatsuya</creator><creator>Sasa, Keita</creator><creator>Okubo, Taketo</creator><creator>Kim, Youngji</creator><creator>Mano, Hiroyuki</creator><creator>Yao, Takashi</creator><creator>Kaneko, Kazuo</creator><creator>Saito, Tsuyoshi</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20201030</creationdate><title>Nanostring-based screening for tyrosine kinase fusions in inflammatory myofibroblastic tumors</title><author>Kurihara, Taisei ; Suehara, Yoshiyuki ; Akaike, Keisuke ; Hayashi, Takuo ; Kohsaka, Shinji ; Ueno, Toshihide ; Hasegawa, Nobuhiko ; Takagi, Tatsuya ; Sasa, Keita ; Okubo, Taketo ; Kim, Youngji ; Mano, Hiroyuki ; Yao, Takashi ; Kaneko, Kazuo ; Saito, Tsuyoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c668t-c8b452f6ace3def1150f07cbbe48d9ecbc24084a95a255c5a1affbd0f708ee63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>631/208</topic><topic>631/67</topic><topic>692/308</topic><topic>692/4017</topic><topic>692/4028</topic><topic>692/420</topic><topic>692/699</topic><topic>Adult</topic><topic>Aged</topic><topic>Antibodies - chemistry</topic><topic>ETS Translocation Variant 6 Protein</topic><topic>Female</topic><topic>Fibronectins - genetics</topic><topic>Fluorescence in situ hybridization</topic><topic>Fusion protein</topic><topic>Gene expression</topic><topic>Gene rearrangement</topic><topic>Humanities and Social Sciences</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Inflammation</topic><topic>Kinases</topic><topic>Lymphoma</topic><topic>Male</topic><topic>Metastases</topic><topic>Middle Aged</topic><topic>multidisciplinary</topic><topic>Myofibroblasts - enzymology</topic><topic>Nanotechnology - methods</topic><topic>Neoplasms - enzymology</topic><topic>Polymerase chain reaction</topic><topic>Protein-tyrosine kinase</topic><topic>Protein-Tyrosine Kinases - genetics</topic><topic>Protein-Tyrosine Kinases - metabolism</topic><topic>Proto-Oncogene Mas</topic><topic>Proto-Oncogene Proteins - genetics</topic><topic>Proto-Oncogene Proteins c-ets - genetics</topic><topic>Receptor, trkC - genetics</topic><topic>Repressor Proteins - genetics</topic><topic>Reverse transcription</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Tropomyosin</topic><topic>Tumors</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kurihara, Taisei</creatorcontrib><creatorcontrib>Suehara, Yoshiyuki</creatorcontrib><creatorcontrib>Akaike, Keisuke</creatorcontrib><creatorcontrib>Hayashi, Takuo</creatorcontrib><creatorcontrib>Kohsaka, Shinji</creatorcontrib><creatorcontrib>Ueno, Toshihide</creatorcontrib><creatorcontrib>Hasegawa, Nobuhiko</creatorcontrib><creatorcontrib>Takagi, Tatsuya</creatorcontrib><creatorcontrib>Sasa, Keita</creatorcontrib><creatorcontrib>Okubo, Taketo</creatorcontrib><creatorcontrib>Kim, Youngji</creatorcontrib><creatorcontrib>Mano, Hiroyuki</creatorcontrib><creatorcontrib>Yao, Takashi</creatorcontrib><creatorcontrib>Kaneko, Kazuo</creatorcontrib><creatorcontrib>Saito, Tsuyoshi</creatorcontrib><collection>SpringerOpen</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest Science Journals</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kurihara, Taisei</au><au>Suehara, Yoshiyuki</au><au>Akaike, Keisuke</au><au>Hayashi, Takuo</au><au>Kohsaka, Shinji</au><au>Ueno, Toshihide</au><au>Hasegawa, Nobuhiko</au><au>Takagi, Tatsuya</au><au>Sasa, Keita</au><au>Okubo, Taketo</au><au>Kim, Youngji</au><au>Mano, Hiroyuki</au><au>Yao, Takashi</au><au>Kaneko, Kazuo</au><au>Saito, Tsuyoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nanostring-based screening for tyrosine kinase fusions in inflammatory myofibroblastic tumors</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2020-10-30</date><risdate>2020</risdate><volume>10</volume><issue>1</issue><spage>18724</spage><epage>18724</epage><pages>18724-18724</pages><artnum>18724</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Gene expression imbalances were measured for tyrosine kinase (
TK
) genes using Nanostring in 19 samples of inflammatory myofibroblastic tumor (IMT). All cases were immunohistochemically stained with anaplastic lymphoma kinase (ALK) and pan-tropomyosin-related-kinase (pan-Trk) antibodies. Five cases with imbalanced
ALK
expression, reported with Nanostring, were tested using fluorescence in situ hybridization (FISH); two cases with imbalanced neurotrophic tyrosine receptor kinase 3 (
NTRK3
) expression were tested using reverse transcription-polymerase chain reaction (RT-PCR). One case with imbalanced expression for ROS proto-oncogene 1 (
ROS1
) was tested using RNA sequencing and RT-PCR. TK fusions were detected in all cases with imbalanced
TK
expression. RNA sequencing detected a
FN1–ROS1
fusion gene in an adult IMT case. IMT with
ALK
rearrangement showed myofibroblast-dominant features. IMT with
ETV6–NTRK3
fusion showed prominent lymphoplasmacytic infiltration with scattered myofibroblasts. Pan-Trk IHC revealed only scattered positively stained cells in IMT with
ETV6–NTRK3
fusion gene.
ROS1
-positive IMT showed myofibroblast-dominant features.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>33127954</pmid><doi>10.1038/s41598-020-75596-3</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2045-2322 |
| ispartof | Scientific reports, 2020-10, Vol.10 (1), p.18724-18724, Article 18724 |
| issn | 2045-2322 2045-2322 |
| language | eng |
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| source | PubMed (Medline); SpringerOpen; MEDLINE; Nature Free; Full-Text Journals in Chemistry (Open access); DOAJ Directory of Open Access Journals; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | 631/208 631/67 692/308 692/4017 692/4028 692/420 692/699 Adult Aged Antibodies - chemistry ETS Translocation Variant 6 Protein Female Fibronectins - genetics Fluorescence in situ hybridization Fusion protein Gene expression Gene rearrangement Humanities and Social Sciences Humans Immunohistochemistry In Situ Hybridization, Fluorescence Inflammation Kinases Lymphoma Male Metastases Middle Aged multidisciplinary Myofibroblasts - enzymology Nanotechnology - methods Neoplasms - enzymology Polymerase chain reaction Protein-tyrosine kinase Protein-Tyrosine Kinases - genetics Protein-Tyrosine Kinases - metabolism Proto-Oncogene Mas Proto-Oncogene Proteins - genetics Proto-Oncogene Proteins c-ets - genetics Receptor, trkC - genetics Repressor Proteins - genetics Reverse transcription Ribonucleic acid RNA Science Science (multidisciplinary) Tropomyosin Tumors Young Adult |
| title | Nanostring-based screening for tyrosine kinase fusions in inflammatory myofibroblastic tumors |
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