The Evaluation of Antibiotic Resistance and nalB Mutants in Pseudomonas eruginosa Isolated from Burnt Patients of Shohada Mehrab Yazd Hospital Burn Ward

Due to extensive damage to the skin, burn victims may acquire life-threatening infections. Though the skin primarily protects against microbial invasions, a large number of bacteria, fungi, and viruses can be isolated from burn patients, specifically , a gram-negative bacterium with both intrinsic and acquired antibiotic resistance (AR) properties. mutations can be found on the in the chromosome. This mutation can induce overexpression of the mexAB-oprMoperon, and affect the MexAB-OprM efflux pump, which removes antimicrobial agents from the bacterial cell. Identifying nalB mutants can be useful for monitoring factors affecting AR. In this study, 70 isolates identified from burn patients and antibacterial sensitivity was evaluated using the Kirby-Bauer method. We also investigated mutations in samples using molecular methods including Polymerase reaction chain (PCR) and Sequencing. We identified mutations in 16 isolates. We also found that the increasing effect of mutants induces hyper production activity of MexAB-OprM resulting in AR. Overall, these findings compliment the findings of previous reports. According to the resistance patterns of the samples, both Amikacin and Ciprofloxacin showed the highest resistance (%). Further, the relationship between Ciprofloxacin resistance and mutations was statistically significant ( = 0.016). The results confirm that the increasing effect of mutants on hyper production activity of MexAB-OprM leads to AR.