Biodegradation of Di (2-Ethylhexyl) Phthalate by a novel Enterobacter spp. Strain YC-IL1 Isolated from Polluted Soil, Mila, Algeria
Di-(2-ethylhexyl) phthalate (DEHP) is one of the phthalic acid ester representatives and is mainly used as a plasticizer to endow polyvinyl chloride plastics with desirable physical properties. It is synthesized in massive amounts worldwide. Many studies have proved the adverse effects of DEHP on hu...
Gespeichert in:
| Veröffentlicht in: | International journal of environmental research and public health 2020-10, Vol.17 (20), p.7501 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 20 |
| container_start_page | 7501 |
| container_title | International journal of environmental research and public health |
| container_volume | 17 |
| creator | Lamraoui, Imane Eltoukhy, Adel Wang, Junhuan Lamraoui, Messaouda Ahmed, Amer Jia, Yang Lu, Tiegang Yan, Yanchun |
| description | Di-(2-ethylhexyl) phthalate (DEHP) is one of the phthalic acid ester representatives and is mainly used as a plasticizer to endow polyvinyl chloride plastics with desirable physical properties. It is synthesized in massive amounts worldwide. Many studies have proved the adverse effects of DEHP on human health and wildlife. DEHP is labeled as an endocrine disruptor which causes human reproductive problems.
spp. YC-IL1, a novel isolated strain from contaminated soil, was identified by 16S rRNA gene analysis and electronic microscope. It is capable of efficiently degrading DEHP (100%) and a wide range of phthalic acid ester PAEs, particularly those containing side chains with branches, or ring structures such as dutylbenzyl phthalate and dicyclohexyl phthalate, which are hard to degrade, with, respectively, 81.15% and 50.69% degradation after 7 days incubation. YC-IL1 is an acido-tolerant strain which remained in pH values lower than pH 5.0 with the optimum pH 7.0 and temperature 30 °C. The DEHP metabolites were detected using HPLC-QQQ and then the degradation pathway was tentatively proposed. Strain YC-IL1 showed high DEHP degradation rate in artificially contaminated soil with 86% removed in 6 days. These results indicate the application potential of YC-IL1 in bioremediation of PAE-polluted sites, even the acidic ones. |
| doi_str_mv | 10.3390/ijerph17207501 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7602616</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2635374180</sourcerecordid><originalsourceid>FETCH-LOGICAL-c418t-8d131b71f017cc6cb806a1276f071e046fdae27706c3d5e9d013f9800beffacc3</originalsourceid><addsrcrecordid>eNpVUU1PGzEUtCpQk9JeOSJLXIrEhvfWib25IEEaIFJQkVIOnCyv1846ctZb7wY15_5xNuJD4TTv6c3MG2kIOUYYMDaGC7cysS5RpCBGgF9IHzmHZMgBD_bmHvnWNCsAlg35-CvpMQaCM4Z98v_ahcIsoypU60JFg6W_HP2ZJtO23PrS_Nv6M_pQtqXyqjU031JFq_BsPJ1WrYkhV7oD2tT1gC7aqFxFnybJbI501oSdpKA2hjV9CN5vdtsiOH9O751X5_TKL0106js5tMo35scbHpHHm-mfyV0y_307m1zNEz3ErE2yAhnmAi2g0JrrPAOuMBXcgkADQ24LZVIhgGtWjMy4AGR2nAHkxlqlNTsil6--9SZfm0KbqgvsZR3dWsWtDMrJz5fKlXIZnqXgkHLkncHpm0EMfzemaeUqbGLVZZYpZyMmupzQsQavLB1D00RjPz4gyF1p8nNpneBkP9cH_b0l9gLoe5Qt</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2635374180</pqid></control><display><type>article</type><title>Biodegradation of Di (2-Ethylhexyl) Phthalate by a novel Enterobacter spp. Strain YC-IL1 Isolated from Polluted Soil, Mila, Algeria</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central Open Access</source><source>MDPI - Multidisciplinary Digital Publishing Institute</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Lamraoui, Imane ; Eltoukhy, Adel ; Wang, Junhuan ; Lamraoui, Messaouda ; Ahmed, Amer ; Jia, Yang ; Lu, Tiegang ; Yan, Yanchun</creator><creatorcontrib>Lamraoui, Imane ; Eltoukhy, Adel ; Wang, Junhuan ; Lamraoui, Messaouda ; Ahmed, Amer ; Jia, Yang ; Lu, Tiegang ; Yan, Yanchun</creatorcontrib><description>Di-(2-ethylhexyl) phthalate (DEHP) is one of the phthalic acid ester representatives and is mainly used as a plasticizer to endow polyvinyl chloride plastics with desirable physical properties. It is synthesized in massive amounts worldwide. Many studies have proved the adverse effects of DEHP on human health and wildlife. DEHP is labeled as an endocrine disruptor which causes human reproductive problems.
spp. YC-IL1, a novel isolated strain from contaminated soil, was identified by 16S rRNA gene analysis and electronic microscope. It is capable of efficiently degrading DEHP (100%) and a wide range of phthalic acid ester PAEs, particularly those containing side chains with branches, or ring structures such as dutylbenzyl phthalate and dicyclohexyl phthalate, which are hard to degrade, with, respectively, 81.15% and 50.69% degradation after 7 days incubation. YC-IL1 is an acido-tolerant strain which remained in pH values lower than pH 5.0 with the optimum pH 7.0 and temperature 30 °C. The DEHP metabolites were detected using HPLC-QQQ and then the degradation pathway was tentatively proposed. Strain YC-IL1 showed high DEHP degradation rate in artificially contaminated soil with 86% removed in 6 days. These results indicate the application potential of YC-IL1 in bioremediation of PAE-polluted sites, even the acidic ones.</description><identifier>ISSN: 1660-4601</identifier><identifier>ISSN: 1661-7827</identifier><identifier>EISSN: 1660-4601</identifier><identifier>DOI: 10.3390/ijerph17207501</identifier><identifier>PMID: 33076331</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Acids ; Algeria ; Biodegradation ; Biodegradation, Environmental ; Bioremediation ; Chain branching ; Chemicals ; Chromatography ; Diethylhexyl Phthalate ; Dioctyl phthalate ; Endocrine disruptors ; Enterobacter ; Enterobacter - genetics ; Environmental degradation ; High-performance liquid chromatography ; Humans ; Incubation ; Interleukin 1 ; Liquid chromatography ; Metabolism ; Metabolites ; Packaging ; Phthalates ; Phthalic acid ; Phthalic Acids ; Physical properties ; Polyvinyl chloride ; Ring structures ; RNA, Ribosomal, 16S - genetics ; rRNA 16S ; Sediment pollution ; Soil ; Soil contamination ; Soil pollution ; Solvents ; Trace elements ; Wildlife</subject><ispartof>International journal of environmental research and public health, 2020-10, Vol.17 (20), p.7501</ispartof><rights>2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 by the authors. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-8d131b71f017cc6cb806a1276f071e046fdae27706c3d5e9d013f9800beffacc3</citedby><cites>FETCH-LOGICAL-c418t-8d131b71f017cc6cb806a1276f071e046fdae27706c3d5e9d013f9800beffacc3</cites><orcidid>0000-0002-0503-4045 ; 0000-0002-9590-7646</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7602616/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7602616/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33076331$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lamraoui, Imane</creatorcontrib><creatorcontrib>Eltoukhy, Adel</creatorcontrib><creatorcontrib>Wang, Junhuan</creatorcontrib><creatorcontrib>Lamraoui, Messaouda</creatorcontrib><creatorcontrib>Ahmed, Amer</creatorcontrib><creatorcontrib>Jia, Yang</creatorcontrib><creatorcontrib>Lu, Tiegang</creatorcontrib><creatorcontrib>Yan, Yanchun</creatorcontrib><title>Biodegradation of Di (2-Ethylhexyl) Phthalate by a novel Enterobacter spp. Strain YC-IL1 Isolated from Polluted Soil, Mila, Algeria</title><title>International journal of environmental research and public health</title><addtitle>Int J Environ Res Public Health</addtitle><description>Di-(2-ethylhexyl) phthalate (DEHP) is one of the phthalic acid ester representatives and is mainly used as a plasticizer to endow polyvinyl chloride plastics with desirable physical properties. It is synthesized in massive amounts worldwide. Many studies have proved the adverse effects of DEHP on human health and wildlife. DEHP is labeled as an endocrine disruptor which causes human reproductive problems.
spp. YC-IL1, a novel isolated strain from contaminated soil, was identified by 16S rRNA gene analysis and electronic microscope. It is capable of efficiently degrading DEHP (100%) and a wide range of phthalic acid ester PAEs, particularly those containing side chains with branches, or ring structures such as dutylbenzyl phthalate and dicyclohexyl phthalate, which are hard to degrade, with, respectively, 81.15% and 50.69% degradation after 7 days incubation. YC-IL1 is an acido-tolerant strain which remained in pH values lower than pH 5.0 with the optimum pH 7.0 and temperature 30 °C. The DEHP metabolites were detected using HPLC-QQQ and then the degradation pathway was tentatively proposed. Strain YC-IL1 showed high DEHP degradation rate in artificially contaminated soil with 86% removed in 6 days. These results indicate the application potential of YC-IL1 in bioremediation of PAE-polluted sites, even the acidic ones.</description><subject>Acids</subject><subject>Algeria</subject><subject>Biodegradation</subject><subject>Biodegradation, Environmental</subject><subject>Bioremediation</subject><subject>Chain branching</subject><subject>Chemicals</subject><subject>Chromatography</subject><subject>Diethylhexyl Phthalate</subject><subject>Dioctyl phthalate</subject><subject>Endocrine disruptors</subject><subject>Enterobacter</subject><subject>Enterobacter - genetics</subject><subject>Environmental degradation</subject><subject>High-performance liquid chromatography</subject><subject>Humans</subject><subject>Incubation</subject><subject>Interleukin 1</subject><subject>Liquid chromatography</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Packaging</subject><subject>Phthalates</subject><subject>Phthalic acid</subject><subject>Phthalic Acids</subject><subject>Physical properties</subject><subject>Polyvinyl chloride</subject><subject>Ring structures</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>rRNA 16S</subject><subject>Sediment pollution</subject><subject>Soil</subject><subject>Soil contamination</subject><subject>Soil pollution</subject><subject>Solvents</subject><subject>Trace elements</subject><subject>Wildlife</subject><issn>1660-4601</issn><issn>1661-7827</issn><issn>1660-4601</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpVUU1PGzEUtCpQk9JeOSJLXIrEhvfWib25IEEaIFJQkVIOnCyv1846ctZb7wY15_5xNuJD4TTv6c3MG2kIOUYYMDaGC7cysS5RpCBGgF9IHzmHZMgBD_bmHvnWNCsAlg35-CvpMQaCM4Z98v_ahcIsoypU60JFg6W_HP2ZJtO23PrS_Nv6M_pQtqXyqjU031JFq_BsPJ1WrYkhV7oD2tT1gC7aqFxFnybJbI501oSdpKA2hjV9CN5vdtsiOH9O751X5_TKL0106js5tMo35scbHpHHm-mfyV0y_307m1zNEz3ErE2yAhnmAi2g0JrrPAOuMBXcgkADQ24LZVIhgGtWjMy4AGR2nAHkxlqlNTsil6--9SZfm0KbqgvsZR3dWsWtDMrJz5fKlXIZnqXgkHLkncHpm0EMfzemaeUqbGLVZZYpZyMmupzQsQavLB1D00RjPz4gyF1p8nNpneBkP9cH_b0l9gLoe5Qt</recordid><startdate>20201015</startdate><enddate>20201015</enddate><creator>Lamraoui, Imane</creator><creator>Eltoukhy, Adel</creator><creator>Wang, Junhuan</creator><creator>Lamraoui, Messaouda</creator><creator>Ahmed, Amer</creator><creator>Jia, Yang</creator><creator>Lu, Tiegang</creator><creator>Yan, Yanchun</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-0503-4045</orcidid><orcidid>https://orcid.org/0000-0002-9590-7646</orcidid></search><sort><creationdate>20201015</creationdate><title>Biodegradation of Di (2-Ethylhexyl) Phthalate by a novel Enterobacter spp. Strain YC-IL1 Isolated from Polluted Soil, Mila, Algeria</title><author>Lamraoui, Imane ; Eltoukhy, Adel ; Wang, Junhuan ; Lamraoui, Messaouda ; Ahmed, Amer ; Jia, Yang ; Lu, Tiegang ; Yan, Yanchun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-8d131b71f017cc6cb806a1276f071e046fdae27706c3d5e9d013f9800beffacc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Acids</topic><topic>Algeria</topic><topic>Biodegradation</topic><topic>Biodegradation, Environmental</topic><topic>Bioremediation</topic><topic>Chain branching</topic><topic>Chemicals</topic><topic>Chromatography</topic><topic>Diethylhexyl Phthalate</topic><topic>Dioctyl phthalate</topic><topic>Endocrine disruptors</topic><topic>Enterobacter</topic><topic>Enterobacter - genetics</topic><topic>Environmental degradation</topic><topic>High-performance liquid chromatography</topic><topic>Humans</topic><topic>Incubation</topic><topic>Interleukin 1</topic><topic>Liquid chromatography</topic><topic>Metabolism</topic><topic>Metabolites</topic><topic>Packaging</topic><topic>Phthalates</topic><topic>Phthalic acid</topic><topic>Phthalic Acids</topic><topic>Physical properties</topic><topic>Polyvinyl chloride</topic><topic>Ring structures</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>rRNA 16S</topic><topic>Sediment pollution</topic><topic>Soil</topic><topic>Soil contamination</topic><topic>Soil pollution</topic><topic>Solvents</topic><topic>Trace elements</topic><topic>Wildlife</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lamraoui, Imane</creatorcontrib><creatorcontrib>Eltoukhy, Adel</creatorcontrib><creatorcontrib>Wang, Junhuan</creatorcontrib><creatorcontrib>Lamraoui, Messaouda</creatorcontrib><creatorcontrib>Ahmed, Amer</creatorcontrib><creatorcontrib>Jia, Yang</creatorcontrib><creatorcontrib>Lu, Tiegang</creatorcontrib><creatorcontrib>Yan, Yanchun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of environmental research and public health</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lamraoui, Imane</au><au>Eltoukhy, Adel</au><au>Wang, Junhuan</au><au>Lamraoui, Messaouda</au><au>Ahmed, Amer</au><au>Jia, Yang</au><au>Lu, Tiegang</au><au>Yan, Yanchun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biodegradation of Di (2-Ethylhexyl) Phthalate by a novel Enterobacter spp. Strain YC-IL1 Isolated from Polluted Soil, Mila, Algeria</atitle><jtitle>International journal of environmental research and public health</jtitle><addtitle>Int J Environ Res Public Health</addtitle><date>2020-10-15</date><risdate>2020</risdate><volume>17</volume><issue>20</issue><spage>7501</spage><pages>7501-</pages><issn>1660-4601</issn><issn>1661-7827</issn><eissn>1660-4601</eissn><abstract>Di-(2-ethylhexyl) phthalate (DEHP) is one of the phthalic acid ester representatives and is mainly used as a plasticizer to endow polyvinyl chloride plastics with desirable physical properties. It is synthesized in massive amounts worldwide. Many studies have proved the adverse effects of DEHP on human health and wildlife. DEHP is labeled as an endocrine disruptor which causes human reproductive problems.
spp. YC-IL1, a novel isolated strain from contaminated soil, was identified by 16S rRNA gene analysis and electronic microscope. It is capable of efficiently degrading DEHP (100%) and a wide range of phthalic acid ester PAEs, particularly those containing side chains with branches, or ring structures such as dutylbenzyl phthalate and dicyclohexyl phthalate, which are hard to degrade, with, respectively, 81.15% and 50.69% degradation after 7 days incubation. YC-IL1 is an acido-tolerant strain which remained in pH values lower than pH 5.0 with the optimum pH 7.0 and temperature 30 °C. The DEHP metabolites were detected using HPLC-QQQ and then the degradation pathway was tentatively proposed. Strain YC-IL1 showed high DEHP degradation rate in artificially contaminated soil with 86% removed in 6 days. These results indicate the application potential of YC-IL1 in bioremediation of PAE-polluted sites, even the acidic ones.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>33076331</pmid><doi>10.3390/ijerph17207501</doi><orcidid>https://orcid.org/0000-0002-0503-4045</orcidid><orcidid>https://orcid.org/0000-0002-9590-7646</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1660-4601 |
| ispartof | International journal of environmental research and public health, 2020-10, Vol.17 (20), p.7501 |
| issn | 1660-4601 1661-7827 1660-4601 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7602616 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; MDPI - Multidisciplinary Digital Publishing Institute; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acids Algeria Biodegradation Biodegradation, Environmental Bioremediation Chain branching Chemicals Chromatography Diethylhexyl Phthalate Dioctyl phthalate Endocrine disruptors Enterobacter Enterobacter - genetics Environmental degradation High-performance liquid chromatography Humans Incubation Interleukin 1 Liquid chromatography Metabolism Metabolites Packaging Phthalates Phthalic acid Phthalic Acids Physical properties Polyvinyl chloride Ring structures RNA, Ribosomal, 16S - genetics rRNA 16S Sediment pollution Soil Soil contamination Soil pollution Solvents Trace elements Wildlife |
| title | Biodegradation of Di (2-Ethylhexyl) Phthalate by a novel Enterobacter spp. Strain YC-IL1 Isolated from Polluted Soil, Mila, Algeria |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-22T19%3A39%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Biodegradation%20of%20Di%20(2-Ethylhexyl)%20Phthalate%20by%20a%20novel%20Enterobacter%20spp.%20Strain%20YC-IL1%20Isolated%20from%20Polluted%20Soil,%20Mila,%20Algeria&rft.jtitle=International%20journal%20of%20environmental%20research%20and%20public%20health&rft.au=Lamraoui,%20Imane&rft.date=2020-10-15&rft.volume=17&rft.issue=20&rft.spage=7501&rft.pages=7501-&rft.issn=1660-4601&rft.eissn=1660-4601&rft_id=info:doi/10.3390/ijerph17207501&rft_dat=%3Cproquest_pubme%3E2635374180%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2635374180&rft_id=info:pmid/33076331&rfr_iscdi=true |