Mesenteric Lymph Duct Drainage Attenuates Lung Inflammatory Injury and Inhibits Endothelial Cell Apoptosis in Septic Rats
The present study was to investigate the effect of mesenteric lymph duct drainage on lung inflammatory response, histological alteration, and endothelial cell apoptosis in septic rats. Animals were randomly assigned into four groups: control, sham surgery, sepsis, and sepsis plus mesenteric lymph dr...
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| Veröffentlicht in: | BioMed research international 2020, Vol.2020 (2020), p.1-12 |
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| creator | Hu, Chunlin Ouyang, Bin Chen, Juan Chen, Minying Guan, Xiangdong Wang, Pingping Ma, Jie Liu, Qier Liu, Ning Sun, Huadong Sun, Qing Chen, Chuanxi Liu, Yongjun Wu, Jianfeng |
| description | The present study was to investigate the effect of mesenteric lymph duct drainage on lung inflammatory response, histological alteration, and endothelial cell apoptosis in septic rats. Animals were randomly assigned into four groups: control, sham surgery, sepsis, and sepsis plus mesenteric lymph drainage. We used the colon ascendens stent peritonitis (CASP) procedure to induce the septic model in rats, and mesenteric lymph drainage was performed with a polyethylene (PE) catheter inserted into mesenteric lymphatic. The animals were sacrificed at the end of CASP in 6 h. The mRNA expression levels of inflammatory mediators were measured by qPCR, and the histologic damage were evaluated by the pathological score method. It was found that mesenteric lymph drainage significantly reduced the expression of TNF-α, IL-1β, and IL-6 mRNA in the lung. Pulmonary interstitial edema and infiltration of inflammatory cells were alleviated by mesenteric lymph drainage. Moreover, increased mRNA levels of TNF-α, IL-1β, IL-6 mRNA, and apoptotic rate were observed in PMVECs treated with septic lymph. These results indicate that mesenteric lymph duct drainage significantly attenuated lung inflammatory injury by decreasing the expression of pivotal inflammatory mediators and inhibiting endothelial apoptosis to preserve the pulmonary barrier function in septic rats. |
| doi_str_mv | 10.1155/2020/3049302 |
| format | Article |
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Animals were randomly assigned into four groups: control, sham surgery, sepsis, and sepsis plus mesenteric lymph drainage. We used the colon ascendens stent peritonitis (CASP) procedure to induce the septic model in rats, and mesenteric lymph drainage was performed with a polyethylene (PE) catheter inserted into mesenteric lymphatic. The animals were sacrificed at the end of CASP in 6 h. The mRNA expression levels of inflammatory mediators were measured by qPCR, and the histologic damage were evaluated by the pathological score method. It was found that mesenteric lymph drainage significantly reduced the expression of TNF-α, IL-1β, and IL-6 mRNA in the lung. Pulmonary interstitial edema and infiltration of inflammatory cells were alleviated by mesenteric lymph drainage. Moreover, increased mRNA levels of TNF-α, IL-1β, IL-6 mRNA, and apoptotic rate were observed in PMVECs treated with septic lymph. These results indicate that mesenteric lymph duct drainage significantly attenuated lung inflammatory injury by decreasing the expression of pivotal inflammatory mediators and inhibiting endothelial apoptosis to preserve the pulmonary barrier function in septic rats.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2020/3049302</identifier><identifier>PMID: 33145344</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Animals ; Apoptosis ; Apoptosis - drug effects ; Apoptosis - genetics ; Biological Factors - pharmacology ; Blood ; Care and treatment ; Catheters ; Colon ; Complications and side effects ; Cytokines ; Damage assessment ; Disease Models, Animal ; Drainage - methods ; Edema ; Endothelial cells ; Endothelial Cells - cytology ; Endothelial Cells - drug effects ; Endothelial Cells - metabolism ; Endothelium ; Gene expression ; Gene Expression Regulation ; Health aspects ; Hemorrhagic shock ; IL-1β ; Implants ; Inflammation ; Inflammatory response ; Interleukin 6 ; Interleukin-1beta - genetics ; Interleukin-1beta - metabolism ; Interleukin-6 - genetics ; Interleukin-6 - metabolism ; Laboratory animals ; Laparotomy ; Liver ; Lung diseases ; Lungs ; Lymph ; Lymph - chemistry ; Lymphatic drainage ; Lymphatic Vessels - metabolism ; Lymphatic Vessels - pathology ; Male ; Medical instruments ; Medical research ; Medicine, Experimental ; Mesentery ; Multiple organ dysfunction syndrome ; Pathogenesis ; Peritonitis ; Peritonitis - complications ; Peritonitis - genetics ; Peritonitis - pathology ; Peritonitis - therapy ; Peroxidase - genetics ; Peroxidase - metabolism ; Pneumonia - complications ; Pneumonia - genetics ; Pneumonia - pathology ; Pneumonia - therapy ; Polyethylene ; Polyethylenes ; Primary Cell Culture ; Pulmonary Edema - complications ; Pulmonary Edema - genetics ; Pulmonary Edema - pathology ; Pulmonary Edema - therapy ; Rats ; Rats, Sprague-Dawley ; Sepsis ; Sepsis - complications ; Sepsis - genetics ; Sepsis - pathology ; Sepsis - therapy ; Surgery ; Surgical drains ; Trauma ; Tumor Necrosis Factor-alpha - genetics ; Tumor Necrosis Factor-alpha - metabolism ; Tumor necrosis factor-α ; Veins & arteries ; Wound drainage</subject><ispartof>BioMed research international, 2020, Vol.2020 (2020), p.1-12</ispartof><rights>Copyright © 2020 Yongjun Liu et al.</rights><rights>COPYRIGHT 2020 John Wiley & Sons, Inc.</rights><rights>Copyright © 2020 Yongjun Liu et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2020 Yongjun Liu et al. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-2328e4e8f2829e3b1467e04a63403ef31b7e3dfe0d98d37c04780fc1ddc6908f3</citedby><cites>FETCH-LOGICAL-c499t-2328e4e8f2829e3b1467e04a63403ef31b7e3dfe0d98d37c04780fc1ddc6908f3</cites><orcidid>0000-0003-1060-2905 ; 0000-0001-9351-4315 ; 0000-0002-5688-4234 ; 0000-0003-4147-933X ; 0000-0001-8246-8763 ; 0000-0003-4165-5292 ; 0000-0002-9510-0957 ; 0000-0003-3027-2253 ; 0000-0001-8173-3393 ; 0000-0001-8735-4335 ; 0000-0001-7880-0719 ; 0000-0001-7646-1311 ; 0000-0001-9098-2500 ; 0000-0002-6402-3389</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7596461/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7596461/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,4024,27923,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33145344$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Niu, Bing</contributor><contributor>Bing Niu</contributor><creatorcontrib>Hu, Chunlin</creatorcontrib><creatorcontrib>Ouyang, Bin</creatorcontrib><creatorcontrib>Chen, Juan</creatorcontrib><creatorcontrib>Chen, Minying</creatorcontrib><creatorcontrib>Guan, Xiangdong</creatorcontrib><creatorcontrib>Wang, Pingping</creatorcontrib><creatorcontrib>Ma, Jie</creatorcontrib><creatorcontrib>Liu, Qier</creatorcontrib><creatorcontrib>Liu, Ning</creatorcontrib><creatorcontrib>Sun, Huadong</creatorcontrib><creatorcontrib>Sun, Qing</creatorcontrib><creatorcontrib>Chen, Chuanxi</creatorcontrib><creatorcontrib>Liu, Yongjun</creatorcontrib><creatorcontrib>Wu, Jianfeng</creatorcontrib><title>Mesenteric Lymph Duct Drainage Attenuates Lung Inflammatory Injury and Inhibits Endothelial Cell Apoptosis in Septic Rats</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>The present study was to investigate the effect of mesenteric lymph duct drainage on lung inflammatory response, histological alteration, and endothelial cell apoptosis in septic rats. Animals were randomly assigned into four groups: control, sham surgery, sepsis, and sepsis plus mesenteric lymph drainage. We used the colon ascendens stent peritonitis (CASP) procedure to induce the septic model in rats, and mesenteric lymph drainage was performed with a polyethylene (PE) catheter inserted into mesenteric lymphatic. The animals were sacrificed at the end of CASP in 6 h. The mRNA expression levels of inflammatory mediators were measured by qPCR, and the histologic damage were evaluated by the pathological score method. It was found that mesenteric lymph drainage significantly reduced the expression of TNF-α, IL-1β, and IL-6 mRNA in the lung. Pulmonary interstitial edema and infiltration of inflammatory cells were alleviated by mesenteric lymph drainage. Moreover, increased mRNA levels of TNF-α, IL-1β, IL-6 mRNA, and apoptotic rate were observed in PMVECs treated with septic lymph. These results indicate that mesenteric lymph duct drainage significantly attenuated lung inflammatory injury by decreasing the expression of pivotal inflammatory mediators and inhibiting endothelial apoptosis to preserve the pulmonary barrier function in septic rats.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Apoptosis - genetics</subject><subject>Biological Factors - pharmacology</subject><subject>Blood</subject><subject>Care and treatment</subject><subject>Catheters</subject><subject>Colon</subject><subject>Complications and side effects</subject><subject>Cytokines</subject><subject>Damage assessment</subject><subject>Disease Models, Animal</subject><subject>Drainage - methods</subject><subject>Edema</subject><subject>Endothelial cells</subject><subject>Endothelial Cells - cytology</subject><subject>Endothelial Cells - drug effects</subject><subject>Endothelial Cells - metabolism</subject><subject>Endothelium</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Health aspects</subject><subject>Hemorrhagic shock</subject><subject>IL-1β</subject><subject>Implants</subject><subject>Inflammation</subject><subject>Inflammatory response</subject><subject>Interleukin 6</subject><subject>Interleukin-1beta - genetics</subject><subject>Interleukin-1beta - metabolism</subject><subject>Interleukin-6 - genetics</subject><subject>Interleukin-6 - metabolism</subject><subject>Laboratory animals</subject><subject>Laparotomy</subject><subject>Liver</subject><subject>Lung diseases</subject><subject>Lungs</subject><subject>Lymph</subject><subject>Lymph - chemistry</subject><subject>Lymphatic drainage</subject><subject>Lymphatic Vessels - metabolism</subject><subject>Lymphatic Vessels - pathology</subject><subject>Male</subject><subject>Medical instruments</subject><subject>Medical research</subject><subject>Medicine, Experimental</subject><subject>Mesentery</subject><subject>Multiple organ dysfunction syndrome</subject><subject>Pathogenesis</subject><subject>Peritonitis</subject><subject>Peritonitis - complications</subject><subject>Peritonitis - genetics</subject><subject>Peritonitis - pathology</subject><subject>Peritonitis - therapy</subject><subject>Peroxidase - genetics</subject><subject>Peroxidase - metabolism</subject><subject>Pneumonia - complications</subject><subject>Pneumonia - genetics</subject><subject>Pneumonia - pathology</subject><subject>Pneumonia - therapy</subject><subject>Polyethylene</subject><subject>Polyethylenes</subject><subject>Primary Cell Culture</subject><subject>Pulmonary Edema - complications</subject><subject>Pulmonary Edema - genetics</subject><subject>Pulmonary Edema - pathology</subject><subject>Pulmonary Edema - therapy</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Sepsis</subject><subject>Sepsis - complications</subject><subject>Sepsis - genetics</subject><subject>Sepsis - pathology</subject><subject>Sepsis - therapy</subject><subject>Surgery</subject><subject>Surgical drains</subject><subject>Trauma</subject><subject>Tumor Necrosis Factor-alpha - genetics</subject><subject>Tumor Necrosis Factor-alpha - metabolism</subject><subject>Tumor necrosis factor-α</subject><subject>Veins & arteries</subject><subject>Wound 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Lymph Duct Drainage Attenuates Lung Inflammatory Injury and Inhibits Endothelial Cell Apoptosis in Septic Rats</title><author>Hu, Chunlin ; Ouyang, Bin ; Chen, Juan ; Chen, Minying ; Guan, Xiangdong ; Wang, Pingping ; Ma, Jie ; Liu, Qier ; Liu, Ning ; Sun, Huadong ; Sun, Qing ; Chen, Chuanxi ; Liu, Yongjun ; Wu, Jianfeng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-2328e4e8f2829e3b1467e04a63403ef31b7e3dfe0d98d37c04780fc1ddc6908f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Apoptosis - genetics</topic><topic>Biological Factors - pharmacology</topic><topic>Blood</topic><topic>Care and treatment</topic><topic>Catheters</topic><topic>Colon</topic><topic>Complications and side effects</topic><topic>Cytokines</topic><topic>Damage assessment</topic><topic>Disease Models, Animal</topic><topic>Drainage - methods</topic><topic>Edema</topic><topic>Endothelial cells</topic><topic>Endothelial Cells - cytology</topic><topic>Endothelial Cells - drug effects</topic><topic>Endothelial Cells - metabolism</topic><topic>Endothelium</topic><topic>Gene expression</topic><topic>Gene Expression Regulation</topic><topic>Health aspects</topic><topic>Hemorrhagic shock</topic><topic>IL-1β</topic><topic>Implants</topic><topic>Inflammation</topic><topic>Inflammatory response</topic><topic>Interleukin 6</topic><topic>Interleukin-1beta - genetics</topic><topic>Interleukin-1beta - metabolism</topic><topic>Interleukin-6 - genetics</topic><topic>Interleukin-6 - metabolism</topic><topic>Laboratory animals</topic><topic>Laparotomy</topic><topic>Liver</topic><topic>Lung diseases</topic><topic>Lungs</topic><topic>Lymph</topic><topic>Lymph - chemistry</topic><topic>Lymphatic drainage</topic><topic>Lymphatic Vessels - metabolism</topic><topic>Lymphatic Vessels - 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Xiangdong</au><au>Wang, Pingping</au><au>Ma, Jie</au><au>Liu, Qier</au><au>Liu, Ning</au><au>Sun, Huadong</au><au>Sun, Qing</au><au>Chen, Chuanxi</au><au>Liu, Yongjun</au><au>Wu, Jianfeng</au><au>Niu, Bing</au><au>Bing Niu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mesenteric Lymph Duct Drainage Attenuates Lung Inflammatory Injury and Inhibits Endothelial Cell Apoptosis in Septic Rats</atitle><jtitle>BioMed research international</jtitle><addtitle>Biomed Res Int</addtitle><date>2020</date><risdate>2020</risdate><volume>2020</volume><issue>2020</issue><spage>1</spage><epage>12</epage><pages>1-12</pages><issn>2314-6133</issn><eissn>2314-6141</eissn><abstract>The present study was to investigate the effect of mesenteric lymph duct drainage on lung inflammatory response, histological alteration, and endothelial cell apoptosis in septic rats. Animals were randomly assigned into four groups: control, sham surgery, sepsis, and sepsis plus mesenteric lymph drainage. We used the colon ascendens stent peritonitis (CASP) procedure to induce the septic model in rats, and mesenteric lymph drainage was performed with a polyethylene (PE) catheter inserted into mesenteric lymphatic. The animals were sacrificed at the end of CASP in 6 h. The mRNA expression levels of inflammatory mediators were measured by qPCR, and the histologic damage were evaluated by the pathological score method. It was found that mesenteric lymph drainage significantly reduced the expression of TNF-α, IL-1β, and IL-6 mRNA in the lung. Pulmonary interstitial edema and infiltration of inflammatory cells were alleviated by mesenteric lymph drainage. Moreover, increased mRNA levels of TNF-α, IL-1β, IL-6 mRNA, and apoptotic rate were observed in PMVECs treated with septic lymph. These results indicate that mesenteric lymph duct drainage significantly attenuated lung inflammatory injury by decreasing the expression of pivotal inflammatory mediators and inhibiting endothelial apoptosis to preserve the pulmonary barrier function in septic rats.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>33145344</pmid><doi>10.1155/2020/3049302</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0003-1060-2905</orcidid><orcidid>https://orcid.org/0000-0001-9351-4315</orcidid><orcidid>https://orcid.org/0000-0002-5688-4234</orcidid><orcidid>https://orcid.org/0000-0003-4147-933X</orcidid><orcidid>https://orcid.org/0000-0001-8246-8763</orcidid><orcidid>https://orcid.org/0000-0003-4165-5292</orcidid><orcidid>https://orcid.org/0000-0002-9510-0957</orcidid><orcidid>https://orcid.org/0000-0003-3027-2253</orcidid><orcidid>https://orcid.org/0000-0001-8173-3393</orcidid><orcidid>https://orcid.org/0000-0001-8735-4335</orcidid><orcidid>https://orcid.org/0000-0001-7880-0719</orcidid><orcidid>https://orcid.org/0000-0001-7646-1311</orcidid><orcidid>https://orcid.org/0000-0001-9098-2500</orcidid><orcidid>https://orcid.org/0000-0002-6402-3389</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2314-6133 |
| ispartof | BioMed research international, 2020, Vol.2020 (2020), p.1-12 |
| issn | 2314-6133 2314-6141 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7596461 |
| source | MEDLINE; PubMed Central Open Access; Wiley Online Library Open Access; PubMed Central; Alma/SFX Local Collection |
| subjects | Animals Apoptosis Apoptosis - drug effects Apoptosis - genetics Biological Factors - pharmacology Blood Care and treatment Catheters Colon Complications and side effects Cytokines Damage assessment Disease Models, Animal Drainage - methods Edema Endothelial cells Endothelial Cells - cytology Endothelial Cells - drug effects Endothelial Cells - metabolism Endothelium Gene expression Gene Expression Regulation Health aspects Hemorrhagic shock IL-1β Implants Inflammation Inflammatory response Interleukin 6 Interleukin-1beta - genetics Interleukin-1beta - metabolism Interleukin-6 - genetics Interleukin-6 - metabolism Laboratory animals Laparotomy Liver Lung diseases Lungs Lymph Lymph - chemistry Lymphatic drainage Lymphatic Vessels - metabolism Lymphatic Vessels - pathology Male Medical instruments Medical research Medicine, Experimental Mesentery Multiple organ dysfunction syndrome Pathogenesis Peritonitis Peritonitis - complications Peritonitis - genetics Peritonitis - pathology Peritonitis - therapy Peroxidase - genetics Peroxidase - metabolism Pneumonia - complications Pneumonia - genetics Pneumonia - pathology Pneumonia - therapy Polyethylene Polyethylenes Primary Cell Culture Pulmonary Edema - complications Pulmonary Edema - genetics Pulmonary Edema - pathology Pulmonary Edema - therapy Rats Rats, Sprague-Dawley Sepsis Sepsis - complications Sepsis - genetics Sepsis - pathology Sepsis - therapy Surgery Surgical drains Trauma Tumor Necrosis Factor-alpha - genetics Tumor Necrosis Factor-alpha - metabolism Tumor necrosis factor-α Veins & arteries Wound drainage |
| title | Mesenteric Lymph Duct Drainage Attenuates Lung Inflammatory Injury and Inhibits Endothelial Cell Apoptosis in Septic Rats |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-28T11%3A11%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Mesenteric%20Lymph%20Duct%20Drainage%20Attenuates%20Lung%20Inflammatory%20Injury%20and%20Inhibits%20Endothelial%20Cell%20Apoptosis%20in%20Septic%20Rats&rft.jtitle=BioMed%20research%20international&rft.au=Hu,%20Chunlin&rft.date=2020&rft.volume=2020&rft.issue=2020&rft.spage=1&rft.epage=12&rft.pages=1-12&rft.issn=2314-6133&rft.eissn=2314-6141&rft_id=info:doi/10.1155/2020/3049302&rft_dat=%3Cgale_pubme%3EA697084085%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2456435488&rft_id=info:pmid/33145344&rft_galeid=A697084085&rfr_iscdi=true |