Mechanoadaptive organization of stress fiber subtypes in epithelial cells under cyclic stretches and stretch release
Cyclic stretch applied to cells induces the reorganization of stress fibers. However, the correlation between the reorganization of stress fiber subtypes and strain-dependent responses of the cytoplasm and nucleus has remained unclear. Here, we investigated the dynamic involvement of stress fiber su...
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description | Cyclic stretch applied to cells induces the reorganization of stress fibers. However, the correlation between the reorganization of stress fiber subtypes and strain-dependent responses of the cytoplasm and nucleus has remained unclear. Here, we investigated the dynamic involvement of stress fiber subtypes in the orientation and elongation of cyclically stretched epithelial cells. We applied uniaxial cyclic stretches at 5%, 10%, and 15% strains to cells followed by the release of the mechanical stretch. Dorsal, transverse arcs, and peripheral stress fibers were mainly involved in the cytoplasm responses whereas perinuclear cap fibers were associated with the reorientation and elongation of the nucleus. Dorsal stress fibers and transverse arcs rapidly responded within 15 min regardless of the strain magnitude to facilitate the subsequent changes in the orientation and elongation of the cytoplasm. The cyclic stretches induced the additional formation of perinuclear cap fibers and their increased number was almost maintained with a slight decline after 2-h-long stretch release. The slow formation and high stability of perinuclear cap fibers were linked to the slow reorientation kinetics and partial morphology recovery of nucleus in the presence or absence of cyclic stretches. The reorganization of stress fiber subtypes occurred in accordance with the reversible distribution of myosin II. These findings allowed us to propose a model for stretch-induced responses of the cytoplasm and nucleus in epithelial cells based on different mechanoadaptive properties of stress fiber subtypes. |
doi_str_mv | 10.1038/s41598-020-75791-2 |
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However, the correlation between the reorganization of stress fiber subtypes and strain-dependent responses of the cytoplasm and nucleus has remained unclear. Here, we investigated the dynamic involvement of stress fiber subtypes in the orientation and elongation of cyclically stretched epithelial cells. We applied uniaxial cyclic stretches at 5%, 10%, and 15% strains to cells followed by the release of the mechanical stretch. Dorsal, transverse arcs, and peripheral stress fibers were mainly involved in the cytoplasm responses whereas perinuclear cap fibers were associated with the reorientation and elongation of the nucleus. Dorsal stress fibers and transverse arcs rapidly responded within 15 min regardless of the strain magnitude to facilitate the subsequent changes in the orientation and elongation of the cytoplasm. The cyclic stretches induced the additional formation of perinuclear cap fibers and their increased number was almost maintained with a slight decline after 2-h-long stretch release. The slow formation and high stability of perinuclear cap fibers were linked to the slow reorientation kinetics and partial morphology recovery of nucleus in the presence or absence of cyclic stretches. The reorganization of stress fiber subtypes occurred in accordance with the reversible distribution of myosin II. These findings allowed us to propose a model for stretch-induced responses of the cytoplasm and nucleus in epithelial cells based on different mechanoadaptive properties of stress fiber subtypes.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-020-75791-2</identifier><identifier>PMID: 33122754</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/57/343/1361 ; 631/80/128 ; 631/80/128/2031 ; A549 Cells ; Animals ; Cytoplasm ; Elasticity ; Elongation ; Epithelial cells ; Epithelial Cells - cytology ; Fibers ; Homeostasis ; Humanities and Social Sciences ; Humans ; Kinetics ; multidisciplinary ; Myosin ; Science ; Science (multidisciplinary) ; Strain ; Stress ; Stress Fibers - physiology ; Stress, Mechanical</subject><ispartof>Scientific reports, 2020-10, Vol.10 (1), p.18684-18684, Article 18684</ispartof><rights>The Author(s) 2020</rights><rights>The Author(s) 2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). 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However, the correlation between the reorganization of stress fiber subtypes and strain-dependent responses of the cytoplasm and nucleus has remained unclear. Here, we investigated the dynamic involvement of stress fiber subtypes in the orientation and elongation of cyclically stretched epithelial cells. We applied uniaxial cyclic stretches at 5%, 10%, and 15% strains to cells followed by the release of the mechanical stretch. Dorsal, transverse arcs, and peripheral stress fibers were mainly involved in the cytoplasm responses whereas perinuclear cap fibers were associated with the reorientation and elongation of the nucleus. Dorsal stress fibers and transverse arcs rapidly responded within 15 min regardless of the strain magnitude to facilitate the subsequent changes in the orientation and elongation of the cytoplasm. The cyclic stretches induced the additional formation of perinuclear cap fibers and their increased number was almost maintained with a slight decline after 2-h-long stretch release. The slow formation and high stability of perinuclear cap fibers were linked to the slow reorientation kinetics and partial morphology recovery of nucleus in the presence or absence of cyclic stretches. The reorganization of stress fiber subtypes occurred in accordance with the reversible distribution of myosin II. These findings allowed us to propose a model for stretch-induced responses of the cytoplasm and nucleus in epithelial cells based on different mechanoadaptive properties of stress fiber subtypes.</description><subject>631/57/343/1361</subject><subject>631/80/128</subject><subject>631/80/128/2031</subject><subject>A549 Cells</subject><subject>Animals</subject><subject>Cytoplasm</subject><subject>Elasticity</subject><subject>Elongation</subject><subject>Epithelial cells</subject><subject>Epithelial Cells - cytology</subject><subject>Fibers</subject><subject>Homeostasis</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Kinetics</subject><subject>multidisciplinary</subject><subject>Myosin</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Strain</subject><subject>Stress</subject><subject>Stress Fibers - physiology</subject><subject>Stress, Mechanical</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kU9vFSEUxYnR2Kb2C7gwJG7cTOXPMDNsTEyj1qTGja7JHbjzHs08GIFp8vrppX1trS5kA-T87oGTQ8hrzs44k8P73HKlh4YJ1vSq17wRz8ixYK1qhBTi-ZPzETnN-YrVpYRuuX5JjqTkQvSqPSblG9othAgOluKvkca0geBvoPgYaJxoLglzppMfMdG8jmW_YKY-UFx82eLsYaYW5znTNbiK2L2dvb0bK3ZbUQju4UYTzggZX5EXE8wZT-_3E_Lz86cf5xfN5fcvX88_XjZWcV4aQIZiAOTouNYwInN27CclxSic6AG0mjptpWgtF9JK6zpQk66KHAbZOXlCPhx8l3XcobMYSoLZLMnvIO1NBG_-VoLfmk28Nr3SHVOqGry7N0jx14q5mJ3Pt2khYFyzEa3qWt5qJiv69h_0Kq4p1HiV6rlqWadYpcSBsinmnHB6_Axn5rZXc-jV1F7NXa9G1KE3T2M8jjy0WAF5AHKVwgbTn7f_Y_sbP3uxZg</recordid><startdate>20201029</startdate><enddate>20201029</enddate><creator>Roshanzadeh, Amir</creator><creator>Nguyen, Tham Thi</creator><creator>Nguyen, Khoa Dang</creator><creator>Kim, Dong-Su</creator><creator>Lee, Bong-Kee</creator><creator>Lee, Dong-Weon</creator><creator>Kim, Eung-Sam</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20201029</creationdate><title>Mechanoadaptive organization of stress fiber subtypes in epithelial cells under cyclic stretches and stretch release</title><author>Roshanzadeh, Amir ; 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However, the correlation between the reorganization of stress fiber subtypes and strain-dependent responses of the cytoplasm and nucleus has remained unclear. Here, we investigated the dynamic involvement of stress fiber subtypes in the orientation and elongation of cyclically stretched epithelial cells. We applied uniaxial cyclic stretches at 5%, 10%, and 15% strains to cells followed by the release of the mechanical stretch. Dorsal, transverse arcs, and peripheral stress fibers were mainly involved in the cytoplasm responses whereas perinuclear cap fibers were associated with the reorientation and elongation of the nucleus. Dorsal stress fibers and transverse arcs rapidly responded within 15 min regardless of the strain magnitude to facilitate the subsequent changes in the orientation and elongation of the cytoplasm. The cyclic stretches induced the additional formation of perinuclear cap fibers and their increased number was almost maintained with a slight decline after 2-h-long stretch release. The slow formation and high stability of perinuclear cap fibers were linked to the slow reorientation kinetics and partial morphology recovery of nucleus in the presence or absence of cyclic stretches. The reorganization of stress fiber subtypes occurred in accordance with the reversible distribution of myosin II. These findings allowed us to propose a model for stretch-induced responses of the cytoplasm and nucleus in epithelial cells based on different mechanoadaptive properties of stress fiber subtypes.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>33122754</pmid><doi>10.1038/s41598-020-75791-2</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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subjects | 631/57/343/1361 631/80/128 631/80/128/2031 A549 Cells Animals Cytoplasm Elasticity Elongation Epithelial cells Epithelial Cells - cytology Fibers Homeostasis Humanities and Social Sciences Humans Kinetics multidisciplinary Myosin Science Science (multidisciplinary) Strain Stress Stress Fibers - physiology Stress, Mechanical |
title | Mechanoadaptive organization of stress fiber subtypes in epithelial cells under cyclic stretches and stretch release |
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