A physiologic overview of the organ-specific transcriptome of the cattle tick Rhipicephalus microplus
To further obtain insights into the Rhipicephalus microplus transcriptome, we used RNA-seq to carry out a study of expression in (i) embryos; (ii) ovaries from partially and fully engorged females; (iii) salivary glands from partially engorged females; (iv) fat body from partially and fully engorged...
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| creator | Tirloni, Lucas Braz, Gloria Nunes, Rodrigo Dutra Gandara, Ana Caroline Paiva Vieira, Larissa Rezende Assumpcao, Teresa Cristina Sabadin, Gabriela Alves da Silva, Renato Martins Guizzo, Melina Garcia Machado, Josias Alves Costa, Evenilton Pessoa Santos, Daniele Gomes, Helga Fernandes Moraes, Jorge dos Santos Mota, Maria Beatriz Mesquita, Rafael Dias de Souza Leite, Milane Alvarenga, Patricia Hessab Lara, Flavio Alves Seixas, Adriana da Fonseca, Rodrigo Nunes Fogaça, Andrea C. Logullo, Carlos Tanaka, Aparecida Sadae Daffre, Sirlei Oliveira, Pedro L. da Silva Vaz, Itabajara Ribeiro, José M. C. |
| description | To further obtain insights into the
Rhipicephalus microplus
transcriptome, we used RNA-seq to carry out a study of expression in (i) embryos; (ii) ovaries from partially and fully engorged females; (iii) salivary glands from partially engorged females; (iv) fat body from partially and fully engorged females; and (v) digestive cells from partially, and (vi) fully engorged females. We obtained > 500 million Illumina reads which were assembled de novo, producing > 190,000 contigs, identifying 18,857 coding sequences (CDS). Reads from each library were mapped back into the assembled transcriptome giving a view of gene expression in different tissues. Transcriptomic expression and pathway analysis showed that several genes related in blood digestion and host-parasite interaction were overexpressed in digestive cells compared with other tissues. Furthermore, essential genes for the cell development and embryogenesis were overexpressed in ovaries. Taken altogether, these data offer novel insights into the physiology of production and role of saliva, blood digestion, energy metabolism, and development with submission of 10,932 novel tissue/cell specific CDS to the NCBI database for this important tick species. |
| doi_str_mv | 10.1038/s41598-020-75341-w |
| format | Article |
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Rhipicephalus microplus
transcriptome, we used RNA-seq to carry out a study of expression in (i) embryos; (ii) ovaries from partially and fully engorged females; (iii) salivary glands from partially engorged females; (iv) fat body from partially and fully engorged females; and (v) digestive cells from partially, and (vi) fully engorged females. We obtained > 500 million Illumina reads which were assembled de novo, producing > 190,000 contigs, identifying 18,857 coding sequences (CDS). Reads from each library were mapped back into the assembled transcriptome giving a view of gene expression in different tissues. Transcriptomic expression and pathway analysis showed that several genes related in blood digestion and host-parasite interaction were overexpressed in digestive cells compared with other tissues. Furthermore, essential genes for the cell development and embryogenesis were overexpressed in ovaries. Taken altogether, these data offer novel insights into the physiology of production and role of saliva, blood digestion, energy metabolism, and development with submission of 10,932 novel tissue/cell specific CDS to the NCBI database for this important tick species.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-020-75341-w</identifier><identifier>PMID: 33106528</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/114 ; 631/337 ; 631/45 ; 631/80 ; Animals ; Cattle ; Embryogenesis ; Energy metabolism ; Fat body ; Female ; Females ; Gene expression ; Gene Expression Profiling - methods ; Gene Expression Regulation ; Gene Regulatory Networks ; High-Throughput Nucleotide Sequencing ; Host-parasite interactions ; Humanities and Social Sciences ; multidisciplinary ; Organ Specificity ; Ovaries ; Ovary - chemistry ; Pregnancy ; Rhipicephalus - genetics ; Rhipicephalus - physiology ; Rhipicephalus microplus ; Ribonucleic acid ; RNA ; Saliva ; Saliva - chemistry ; Salivary gland ; Science ; Science (multidisciplinary) ; Sequence Analysis, RNA ; Transcriptomics</subject><ispartof>Scientific reports, 2020-10, Vol.10 (1), p.18296-18296, Article 18296</ispartof><rights>The Author(s) 2020</rights><rights>The Author(s) 2020. 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C.</creatorcontrib><title>A physiologic overview of the organ-specific transcriptome of the cattle tick Rhipicephalus microplus</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>To further obtain insights into the
Rhipicephalus microplus
transcriptome, we used RNA-seq to carry out a study of expression in (i) embryos; (ii) ovaries from partially and fully engorged females; (iii) salivary glands from partially engorged females; (iv) fat body from partially and fully engorged females; and (v) digestive cells from partially, and (vi) fully engorged females. We obtained > 500 million Illumina reads which were assembled de novo, producing > 190,000 contigs, identifying 18,857 coding sequences (CDS). Reads from each library were mapped back into the assembled transcriptome giving a view of gene expression in different tissues. Transcriptomic expression and pathway analysis showed that several genes related in blood digestion and host-parasite interaction were overexpressed in digestive cells compared with other tissues. Furthermore, essential genes for the cell development and embryogenesis were overexpressed in ovaries. 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C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A physiologic overview of the organ-specific transcriptome of the cattle tick Rhipicephalus microplus</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2020-10-26</date><risdate>2020</risdate><volume>10</volume><issue>1</issue><spage>18296</spage><epage>18296</epage><pages>18296-18296</pages><artnum>18296</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>To further obtain insights into the
Rhipicephalus microplus
transcriptome, we used RNA-seq to carry out a study of expression in (i) embryos; (ii) ovaries from partially and fully engorged females; (iii) salivary glands from partially engorged females; (iv) fat body from partially and fully engorged females; and (v) digestive cells from partially, and (vi) fully engorged females. We obtained > 500 million Illumina reads which were assembled de novo, producing > 190,000 contigs, identifying 18,857 coding sequences (CDS). Reads from each library were mapped back into the assembled transcriptome giving a view of gene expression in different tissues. Transcriptomic expression and pathway analysis showed that several genes related in blood digestion and host-parasite interaction were overexpressed in digestive cells compared with other tissues. Furthermore, essential genes for the cell development and embryogenesis were overexpressed in ovaries. Taken altogether, these data offer novel insights into the physiology of production and role of saliva, blood digestion, energy metabolism, and development with submission of 10,932 novel tissue/cell specific CDS to the NCBI database for this important tick species.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>33106528</pmid><doi>10.1038/s41598-020-75341-w</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7588415 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Nature Free; PubMed Central; Alma/SFX Local Collection; Springer Nature OA/Free Journals; Free Full-Text Journals in Chemistry |
| subjects | 631/114 631/337 631/45 631/80 Animals Cattle Embryogenesis Energy metabolism Fat body Female Females Gene expression Gene Expression Profiling - methods Gene Expression Regulation Gene Regulatory Networks High-Throughput Nucleotide Sequencing Host-parasite interactions Humanities and Social Sciences multidisciplinary Organ Specificity Ovaries Ovary - chemistry Pregnancy Rhipicephalus - genetics Rhipicephalus - physiology Rhipicephalus microplus Ribonucleic acid RNA Saliva Saliva - chemistry Salivary gland Science Science (multidisciplinary) Sequence Analysis, RNA Transcriptomics |
| title | A physiologic overview of the organ-specific transcriptome of the cattle tick Rhipicephalus microplus |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-18T09%3A10%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20physiologic%20overview%20of%20the%20organ-specific%20transcriptome%20of%20the%20cattle%20tick%20Rhipicephalus%20microplus&rft.jtitle=Scientific%20reports&rft.au=Tirloni,%20Lucas&rft.date=2020-10-26&rft.volume=10&rft.issue=1&rft.spage=18296&rft.epage=18296&rft.pages=18296-18296&rft.artnum=18296&rft.issn=2045-2322&rft.eissn=2045-2322&rft_id=info:doi/10.1038/s41598-020-75341-w&rft_dat=%3Cproquest_pubme%3E2454654725%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2471522557&rft_id=info:pmid/33106528&rfr_iscdi=true |