The antiandrogenic vinclozolin induces differentiation delay of germ cells and changes in energy metabolism in 3D cultures of fetal ovaries
Vinclozolin is a pesticide with antiandrogenic activity as an endocrine disruptor compound. Its effects upon the progression of primordial follicles were assessed in cultures of mouse fetal ovaries from the onset of meiotic differentiation of germ cells (13.5 days post coitum) and from both in vivo...
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| Veröffentlicht in: | Scientific reports 2020-10, Vol.10 (1), p.18036-18036, Article 18036 |
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| creator | González-Sanz, Silvia Barreñada, Odei Rial, Eduardo Brieño-Enriquez, Miguel A. del Mazo, Jesús |
| description | Vinclozolin is a pesticide with antiandrogenic activity as an endocrine disruptor compound. Its effects upon the progression of primordial follicles were assessed in cultures of mouse fetal ovaries from the onset of meiotic differentiation of germ cells (13.5 days post coitum) and from both in vivo exposed mice and in vitro exposed ovaries. Exposure of ovaries to vinclozolin—at in vitro dosages ranging from 10 to 200 μM and in 3D ex vivo culture following in vivo exposure to 50 mg/kg bw/day—showed delays in meiocyte differentiation and in follicle growth, even at the lowest in vitro dose exposure. Immunofluorescent analysis showed the presence of the proteins MSY2 and NOBOX in the primary follicles but no difference in the level of protein signals or in the number of follicles in relation to treatment. However, assessing the cytological differentiation of germ cells by detecting the synaptonemal complex protein SYCP3, the exposure to vinclozolin delayed meiotic differentiation from both in vitro- and in vivo-exposed ovaries. These effects were concomitant with changes in the energy metabolism, detected as a relative increase of glycolytic metabolism in live-cell metabolic assays in exposed ovaries. |
| doi_str_mv | 10.1038/s41598-020-75116-3 |
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Its effects upon the progression of primordial follicles were assessed in cultures of mouse fetal ovaries from the onset of meiotic differentiation of germ cells (13.5 days post coitum) and from both in vivo exposed mice and in vitro exposed ovaries. Exposure of ovaries to vinclozolin—at in vitro dosages ranging from 10 to 200 μM and in 3D ex vivo culture following in vivo exposure to 50 mg/kg bw/day—showed delays in meiocyte differentiation and in follicle growth, even at the lowest in vitro dose exposure. Immunofluorescent analysis showed the presence of the proteins MSY2 and NOBOX in the primary follicles but no difference in the level of protein signals or in the number of follicles in relation to treatment. However, assessing the cytological differentiation of germ cells by detecting the synaptonemal complex protein SYCP3, the exposure to vinclozolin delayed meiotic differentiation from both in vitro- and in vivo-exposed ovaries. These effects were concomitant with changes in the energy metabolism, detected as a relative increase of glycolytic metabolism in live-cell metabolic assays in exposed ovaries.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-020-75116-3</identifier><identifier>PMID: 33093579</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/136 ; 631/337 ; 631/80 ; 704/172 ; Androgen Antagonists - pharmacology ; Animals ; Antiandrogenic activity ; Cell culture ; Cell Differentiation ; Dosage ; Energy metabolism ; Energy Metabolism - drug effects ; Female ; Fetus - cytology ; Fetus - drug effects ; Fetus - metabolism ; Fetuses ; Follicles ; Germ cells ; Germ Cells - cytology ; Germ Cells - drug effects ; Germ Cells - metabolism ; Glycolysis ; Humanities and Social Sciences ; Meiosis ; Metabolism ; Mice ; multidisciplinary ; Ovaries ; Ovary - cytology ; Ovary - drug effects ; Ovary - metabolism ; Oxazoles - pharmacology ; Pesticides ; Science ; Science (multidisciplinary) ; Synaptonemal complex ; Vinclozolin</subject><ispartof>Scientific reports, 2020-10, Vol.10 (1), p.18036-18036, Article 18036</ispartof><rights>The Author(s) 2020</rights><rights>The Author(s) 2020. 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>González-Sanz, Silvia</au><au>Barreñada, Odei</au><au>Rial, Eduardo</au><au>Brieño-Enriquez, Miguel A.</au><au>del Mazo, Jesús</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The antiandrogenic vinclozolin induces differentiation delay of germ cells and changes in energy metabolism in 3D cultures of fetal ovaries</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2020-10-22</date><risdate>2020</risdate><volume>10</volume><issue>1</issue><spage>18036</spage><epage>18036</epage><pages>18036-18036</pages><artnum>18036</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Vinclozolin is a pesticide with antiandrogenic activity as an endocrine disruptor compound. Its effects upon the progression of primordial follicles were assessed in cultures of mouse fetal ovaries from the onset of meiotic differentiation of germ cells (13.5 days post coitum) and from both in vivo exposed mice and in vitro exposed ovaries. Exposure of ovaries to vinclozolin—at in vitro dosages ranging from 10 to 200 μM and in 3D ex vivo culture following in vivo exposure to 50 mg/kg bw/day—showed delays in meiocyte differentiation and in follicle growth, even at the lowest in vitro dose exposure. Immunofluorescent analysis showed the presence of the proteins MSY2 and NOBOX in the primary follicles but no difference in the level of protein signals or in the number of follicles in relation to treatment. However, assessing the cytological differentiation of germ cells by detecting the synaptonemal complex protein SYCP3, the exposure to vinclozolin delayed meiotic differentiation from both in vitro- and in vivo-exposed ovaries. These effects were concomitant with changes in the energy metabolism, detected as a relative increase of glycolytic metabolism in live-cell metabolic assays in exposed ovaries.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>33093579</pmid><doi>10.1038/s41598-020-75116-3</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 631/136 631/337 631/80 704/172 Androgen Antagonists - pharmacology Animals Antiandrogenic activity Cell culture Cell Differentiation Dosage Energy metabolism Energy Metabolism - drug effects Female Fetus - cytology Fetus - drug effects Fetus - metabolism Fetuses Follicles Germ cells Germ Cells - cytology Germ Cells - drug effects Germ Cells - metabolism Glycolysis Humanities and Social Sciences Meiosis Metabolism Mice multidisciplinary Ovaries Ovary - cytology Ovary - drug effects Ovary - metabolism Oxazoles - pharmacology Pesticides Science Science (multidisciplinary) Synaptonemal complex Vinclozolin |
| title | The antiandrogenic vinclozolin induces differentiation delay of germ cells and changes in energy metabolism in 3D cultures of fetal ovaries |
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