Hypomethylation of the DAZ3 promoter in idiopathic asthenospermia: a screening tool for liquid biopsy
Given the role of the deleted in azoospermia gene in male infertility, whether the somatic deleted in azoospermia methylation status is associated with idiopathic asthenospermia should be determined. To investigate the methylation levels of the deleted in azoospermia promoter in peripheral white blo...
Gespeichert in:
| Veröffentlicht in: | Scientific reports 2020-10, Vol.10 (1), p.17996-17996, Article 17996 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 17996 |
|---|---|
| container_issue | 1 |
| container_start_page | 17996 |
| container_title | Scientific reports |
| container_volume | 10 |
| creator | Zhang, Shichang Xu, Li Yu, Mengyao Zhang, Jiexin |
| description | Given the role of the
deleted in azoospermia
gene in male infertility, whether the somatic
deleted in azoospermia
methylation status is associated with idiopathic asthenospermia should be determined. To investigate the methylation levels of the
deleted in azoospermia
promoter in peripheral white blood cells from idiopathic asthenospermia patients relative to those in normozoospermia controls, 61 ethylene diamine tetraacetic acid anticoagulant blood samples were drawn from all participants for DNA isolation. The
deleted in azoospermia
promoter methylation ratio was detected by MassARRAY-based methylation quantification and confirmed by quantitative methylation-specific polymerase chain reaction. A MassARRAY-based methylation analysis showed that the
deleted in azoospermia 3
promoter (0 to − 2 kbp) was significantly hypomethylated in peripheral white blood cells from idiopathic asthenospermia males, specifically one CpG site (− 246 to − 247). Quantitative methylation-specific polymerase chain reaction data further confirmed that the methylation level of the
deleted in azoospermia 3
promoter region in idiopathic asthenospermia patients was significantly lower than that in normozoospermia males. The area under the receiver operating characteristic curve determined by quantitative methylation-specific polymerase chain reaction was 0.737 (95% confidence interval: 0.552 to 0.924), with a sensitivity of 53.9% and a specificity of 88.2% at a cut-off level of 74.7%. Therefore, our results suggested that methylation ratio detection of the
deleted in azoospermia 3
promoter region by real-time polymerase chain reaction assay is a promising and feasible tool for liquid biopsy in the clinical laboratories. The methylation status of other reported infertility-related genes should also be investigated in peripheral white blood cells. |
| doi_str_mv | 10.1038/s41598-020-75110-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7581813</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2454105005</sourcerecordid><originalsourceid>FETCH-LOGICAL-c474t-a8e232757ec1d6cad350d254d20534318dba48e878f8583250c957a1d2dc07023</originalsourceid><addsrcrecordid>eNp9kT9vFDEQxS1ERKKQL0CBLNHQbPDfs5cCKQqEIEVKExoay2fP3jnatTe2N9J9ewyXhECBG1ua37yZ54fQG0pOKeH6QxFU9rojjHRKUkq6_gU6YkTIjnHGXj57H6KTUm5JO5L1gvav0CHnpOcryo8QXO7mNEHd7kZbQ4o4DbhuAX8--8HxnNOUKmQcIg4-pNnWbXDYlkbEVGbIU7AfscXFZYAY4gbXlEY8pIzHcLcEj9etq-xeo4PBjgVOHu5j9P3iy835ZXd1_fXb-dlV54QStbMa2sJKKnDUr5z1XBLPpPCMSC441X5thQat9KCl5kwS10tlqWfeEUUYP0af9rrzsp7AO4g129HMOUw270yywfxdiWFrNuneKKmpprwJvH8QyOlugVLNFIqDcbQR0lIME1JQIttXNvTdP-htWnJs9hqlqGxO5KpRbE-5nErJMDwtQ4n5FaTZB2lakOZ3kKZvTW-f23hqeYytAXwPlFaKG8h_Zv9H9iew9ak5</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2471523256</pqid></control><display><type>article</type><title>Hypomethylation of the DAZ3 promoter in idiopathic asthenospermia: a screening tool for liquid biopsy</title><source>MEDLINE</source><source>Nature Free</source><source>Full-Text Journals in Chemistry (Open access)</source><source>PubMed Central</source><source>Directory of Open Access Journals</source><source>Alma/SFX Local Collection</source><source>EZB Electronic Journals Library</source><source>Springer Nature OA Free Journals</source><creator>Zhang, Shichang ; Xu, Li ; Yu, Mengyao ; Zhang, Jiexin</creator><creatorcontrib>Zhang, Shichang ; Xu, Li ; Yu, Mengyao ; Zhang, Jiexin</creatorcontrib><description>Given the role of the
deleted in azoospermia
gene in male infertility, whether the somatic
deleted in azoospermia
methylation status is associated with idiopathic asthenospermia should be determined. To investigate the methylation levels of the
deleted in azoospermia
promoter in peripheral white blood cells from idiopathic asthenospermia patients relative to those in normozoospermia controls, 61 ethylene diamine tetraacetic acid anticoagulant blood samples were drawn from all participants for DNA isolation. The
deleted in azoospermia
promoter methylation ratio was detected by MassARRAY-based methylation quantification and confirmed by quantitative methylation-specific polymerase chain reaction. A MassARRAY-based methylation analysis showed that the
deleted in azoospermia 3
promoter (0 to − 2 kbp) was significantly hypomethylated in peripheral white blood cells from idiopathic asthenospermia males, specifically one CpG site (− 246 to − 247). Quantitative methylation-specific polymerase chain reaction data further confirmed that the methylation level of the
deleted in azoospermia 3
promoter region in idiopathic asthenospermia patients was significantly lower than that in normozoospermia males. The area under the receiver operating characteristic curve determined by quantitative methylation-specific polymerase chain reaction was 0.737 (95% confidence interval: 0.552 to 0.924), with a sensitivity of 53.9% and a specificity of 88.2% at a cut-off level of 74.7%. Therefore, our results suggested that methylation ratio detection of the
deleted in azoospermia 3
promoter region by real-time polymerase chain reaction assay is a promising and feasible tool for liquid biopsy in the clinical laboratories. The methylation status of other reported infertility-related genes should also be investigated in peripheral white blood cells.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-020-75110-9</identifier><identifier>PMID: 33093613</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/208/721 ; 692/699/2732 ; Adult ; Asthenozoospermia - diagnosis ; Asthenozoospermia - genetics ; Biopsy ; Blood ; Cohort Studies ; CpG islands ; DNA - analysis ; DNA - chemistry ; DNA - genetics ; DNA Methylation ; Epigenesis, Genetic ; Humanities and Social Sciences ; Humans ; Infertility ; Leukocytes ; Liquid Biopsy - methods ; Male ; Males ; Methylation ; multidisciplinary ; Polymerase chain reaction ; Promoter Regions, Genetic ; Real-Time Polymerase Chain Reaction ; Science ; Science (multidisciplinary)</subject><ispartof>Scientific reports, 2020-10, Vol.10 (1), p.17996-17996, Article 17996</ispartof><rights>The Author(s) 2020</rights><rights>The Author(s) 2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-a8e232757ec1d6cad350d254d20534318dba48e878f8583250c957a1d2dc07023</citedby><cites>FETCH-LOGICAL-c474t-a8e232757ec1d6cad350d254d20534318dba48e878f8583250c957a1d2dc07023</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7581813/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7581813/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,41096,42165,51551,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33093613$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Shichang</creatorcontrib><creatorcontrib>Xu, Li</creatorcontrib><creatorcontrib>Yu, Mengyao</creatorcontrib><creatorcontrib>Zhang, Jiexin</creatorcontrib><title>Hypomethylation of the DAZ3 promoter in idiopathic asthenospermia: a screening tool for liquid biopsy</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Given the role of the
deleted in azoospermia
gene in male infertility, whether the somatic
deleted in azoospermia
methylation status is associated with idiopathic asthenospermia should be determined. To investigate the methylation levels of the
deleted in azoospermia
promoter in peripheral white blood cells from idiopathic asthenospermia patients relative to those in normozoospermia controls, 61 ethylene diamine tetraacetic acid anticoagulant blood samples were drawn from all participants for DNA isolation. The
deleted in azoospermia
promoter methylation ratio was detected by MassARRAY-based methylation quantification and confirmed by quantitative methylation-specific polymerase chain reaction. A MassARRAY-based methylation analysis showed that the
deleted in azoospermia 3
promoter (0 to − 2 kbp) was significantly hypomethylated in peripheral white blood cells from idiopathic asthenospermia males, specifically one CpG site (− 246 to − 247). Quantitative methylation-specific polymerase chain reaction data further confirmed that the methylation level of the
deleted in azoospermia 3
promoter region in idiopathic asthenospermia patients was significantly lower than that in normozoospermia males. The area under the receiver operating characteristic curve determined by quantitative methylation-specific polymerase chain reaction was 0.737 (95% confidence interval: 0.552 to 0.924), with a sensitivity of 53.9% and a specificity of 88.2% at a cut-off level of 74.7%. Therefore, our results suggested that methylation ratio detection of the
deleted in azoospermia 3
promoter region by real-time polymerase chain reaction assay is a promising and feasible tool for liquid biopsy in the clinical laboratories. The methylation status of other reported infertility-related genes should also be investigated in peripheral white blood cells.</description><subject>631/208/721</subject><subject>692/699/2732</subject><subject>Adult</subject><subject>Asthenozoospermia - diagnosis</subject><subject>Asthenozoospermia - genetics</subject><subject>Biopsy</subject><subject>Blood</subject><subject>Cohort Studies</subject><subject>CpG islands</subject><subject>DNA - analysis</subject><subject>DNA - chemistry</subject><subject>DNA - genetics</subject><subject>DNA Methylation</subject><subject>Epigenesis, Genetic</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Infertility</subject><subject>Leukocytes</subject><subject>Liquid Biopsy - methods</subject><subject>Male</subject><subject>Males</subject><subject>Methylation</subject><subject>multidisciplinary</subject><subject>Polymerase chain reaction</subject><subject>Promoter Regions, Genetic</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kT9vFDEQxS1ERKKQL0CBLNHQbPDfs5cCKQqEIEVKExoay2fP3jnatTe2N9J9ewyXhECBG1ua37yZ54fQG0pOKeH6QxFU9rojjHRKUkq6_gU6YkTIjnHGXj57H6KTUm5JO5L1gvav0CHnpOcryo8QXO7mNEHd7kZbQ4o4DbhuAX8--8HxnNOUKmQcIg4-pNnWbXDYlkbEVGbIU7AfscXFZYAY4gbXlEY8pIzHcLcEj9etq-xeo4PBjgVOHu5j9P3iy835ZXd1_fXb-dlV54QStbMa2sJKKnDUr5z1XBLPpPCMSC441X5thQat9KCl5kwS10tlqWfeEUUYP0af9rrzsp7AO4g129HMOUw270yywfxdiWFrNuneKKmpprwJvH8QyOlugVLNFIqDcbQR0lIME1JQIttXNvTdP-htWnJs9hqlqGxO5KpRbE-5nErJMDwtQ4n5FaTZB2lakOZ3kKZvTW-f23hqeYytAXwPlFaKG8h_Zv9H9iew9ak5</recordid><startdate>20201022</startdate><enddate>20201022</enddate><creator>Zhang, Shichang</creator><creator>Xu, Li</creator><creator>Yu, Mengyao</creator><creator>Zhang, Jiexin</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20201022</creationdate><title>Hypomethylation of the DAZ3 promoter in idiopathic asthenospermia: a screening tool for liquid biopsy</title><author>Zhang, Shichang ; Xu, Li ; Yu, Mengyao ; Zhang, Jiexin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-a8e232757ec1d6cad350d254d20534318dba48e878f8583250c957a1d2dc07023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>631/208/721</topic><topic>692/699/2732</topic><topic>Adult</topic><topic>Asthenozoospermia - diagnosis</topic><topic>Asthenozoospermia - genetics</topic><topic>Biopsy</topic><topic>Blood</topic><topic>Cohort Studies</topic><topic>CpG islands</topic><topic>DNA - analysis</topic><topic>DNA - chemistry</topic><topic>DNA - genetics</topic><topic>DNA Methylation</topic><topic>Epigenesis, Genetic</topic><topic>Humanities and Social Sciences</topic><topic>Humans</topic><topic>Infertility</topic><topic>Leukocytes</topic><topic>Liquid Biopsy - methods</topic><topic>Male</topic><topic>Males</topic><topic>Methylation</topic><topic>multidisciplinary</topic><topic>Polymerase chain reaction</topic><topic>Promoter Regions, Genetic</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Shichang</creatorcontrib><creatorcontrib>Xu, Li</creatorcontrib><creatorcontrib>Yu, Mengyao</creatorcontrib><creatorcontrib>Zhang, Jiexin</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest Science Journals</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Shichang</au><au>Xu, Li</au><au>Yu, Mengyao</au><au>Zhang, Jiexin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hypomethylation of the DAZ3 promoter in idiopathic asthenospermia: a screening tool for liquid biopsy</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2020-10-22</date><risdate>2020</risdate><volume>10</volume><issue>1</issue><spage>17996</spage><epage>17996</epage><pages>17996-17996</pages><artnum>17996</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Given the role of the
deleted in azoospermia
gene in male infertility, whether the somatic
deleted in azoospermia
methylation status is associated with idiopathic asthenospermia should be determined. To investigate the methylation levels of the
deleted in azoospermia
promoter in peripheral white blood cells from idiopathic asthenospermia patients relative to those in normozoospermia controls, 61 ethylene diamine tetraacetic acid anticoagulant blood samples were drawn from all participants for DNA isolation. The
deleted in azoospermia
promoter methylation ratio was detected by MassARRAY-based methylation quantification and confirmed by quantitative methylation-specific polymerase chain reaction. A MassARRAY-based methylation analysis showed that the
deleted in azoospermia 3
promoter (0 to − 2 kbp) was significantly hypomethylated in peripheral white blood cells from idiopathic asthenospermia males, specifically one CpG site (− 246 to − 247). Quantitative methylation-specific polymerase chain reaction data further confirmed that the methylation level of the
deleted in azoospermia 3
promoter region in idiopathic asthenospermia patients was significantly lower than that in normozoospermia males. The area under the receiver operating characteristic curve determined by quantitative methylation-specific polymerase chain reaction was 0.737 (95% confidence interval: 0.552 to 0.924), with a sensitivity of 53.9% and a specificity of 88.2% at a cut-off level of 74.7%. Therefore, our results suggested that methylation ratio detection of the
deleted in azoospermia 3
promoter region by real-time polymerase chain reaction assay is a promising and feasible tool for liquid biopsy in the clinical laboratories. The methylation status of other reported infertility-related genes should also be investigated in peripheral white blood cells.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>33093613</pmid><doi>10.1038/s41598-020-75110-9</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2045-2322 |
| ispartof | Scientific reports, 2020-10, Vol.10 (1), p.17996-17996, Article 17996 |
| issn | 2045-2322 2045-2322 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7581813 |
| source | MEDLINE; Nature Free; Full-Text Journals in Chemistry (Open access); PubMed Central; Directory of Open Access Journals; Alma/SFX Local Collection; EZB Electronic Journals Library; Springer Nature OA Free Journals |
| subjects | 631/208/721 692/699/2732 Adult Asthenozoospermia - diagnosis Asthenozoospermia - genetics Biopsy Blood Cohort Studies CpG islands DNA - analysis DNA - chemistry DNA - genetics DNA Methylation Epigenesis, Genetic Humanities and Social Sciences Humans Infertility Leukocytes Liquid Biopsy - methods Male Males Methylation multidisciplinary Polymerase chain reaction Promoter Regions, Genetic Real-Time Polymerase Chain Reaction Science Science (multidisciplinary) |
| title | Hypomethylation of the DAZ3 promoter in idiopathic asthenospermia: a screening tool for liquid biopsy |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-14T18%3A57%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Hypomethylation%20of%20the%20DAZ3%20promoter%20in%20idiopathic%20asthenospermia:%20a%20screening%20tool%20for%20liquid%20biopsy&rft.jtitle=Scientific%20reports&rft.au=Zhang,%20Shichang&rft.date=2020-10-22&rft.volume=10&rft.issue=1&rft.spage=17996&rft.epage=17996&rft.pages=17996-17996&rft.artnum=17996&rft.issn=2045-2322&rft.eissn=2045-2322&rft_id=info:doi/10.1038/s41598-020-75110-9&rft_dat=%3Cproquest_pubme%3E2454105005%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2471523256&rft_id=info:pmid/33093613&rfr_iscdi=true |