Houttuynia cordata Extract Ameliorates Bladder Damage and Improves Bladder Symptoms via Anti-Inflammatory Effect in Rats with Interstitial Cystitis
The mechanism of interstitial cystitis/bladder pain syndrome (IC/BPS) remains unclear to date, but reports showed that bladder inflammation and increasing number of activating mast cells in bladder tissues were common in patients with IC/BPS. Houttuynia cordata is widely used in Chinese traditional...
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| description | The mechanism of interstitial cystitis/bladder pain syndrome (IC/BPS) remains unclear to date, but reports showed that bladder inflammation and increasing number of activating mast cells in bladder tissues were common in patients with IC/BPS. Houttuynia cordata is widely used in Chinese traditional medicine, and its function of anti-inflammation has been proved. The purpose of this study was to investigate the efficacy and possible mechanisms of the Houttuynia cordata (HC) extract in the treatment of interstitial cystitis/bladder pain syndrome (IC/BPS). In the current study, a total of 30 adult female rats were randomly divided into three groups: sham group (n = 10), cyclophosphamide + saline (CYP + NS) group (n = 10), and cyclophosphamide + Houttuynia cordata extract (CYP + HC) group (n = 10). The animal model of IC/BPS was induced with cyclophosphamide (75 mg/kg, intraperitoneal injection, once every 3 days for 10 days) in the CYP + NS group and CYP + HC group, and sham rats received a volume-matched injection of saline. After anesthesia with urethane (0.8 g/kg, intraperitoneal injection), intravesical administration of either saline (1 ml) or Houttuynia cordata extract (1 ml, 2 g/ml) was continued once per day for a week in the CYP + NS group and CYP + HC group, respectively. Subsequently, urinary frequency, nociceptive behaviors, cystometry, bladder weight, histological changes, and cytokine (IL-6, IL-8, TNF-α) concentration were evaluated and compared among the three groups. Variables including inflammatory grade, mast cell number, proportion of activated mast cells, bladder weight, cytokine concentration of bladder homogenates, and frequency of urination significantly increased in the CYP + NS group compared with the sham group (P |
| doi_str_mv | 10.1155/2020/9026901 |
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| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7568804</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A697087638</galeid><sourcerecordid>A697087638</sourcerecordid><originalsourceid>FETCH-LOGICAL-c476t-5a04bd182301cb0baef9751092993472f1ce02f89725aeca9abb2f28611113423</originalsourceid><addsrcrecordid>eNqFks-L1DAUx4so7rp68ywBL4LWTdKmaS7CODu6AwuCP8BbeG2TmSxtMibp7Pbv8B82dYZd9WIuefA-fMh7-WbZc4LfEsLYOcUUnwtMK4HJg-yU8JLkJa3rh3c1_36SPQnhGmMqOOePs5OiIEVBiTjNfl66McZxsgZQ63wHEdDqNnpoI1oMqjfOQ1QBve-h65RHFzDARiGwHVoPO-_2f_S-TMMuuiGgfZItbDT52uoehgGi8xNaaa2S1Vj0GWJANyZu0dpG5UM00UCPltPvKjzNHmnog3p2vM-ybx9WX5eX-dWnj-vl4ipvS17FnAEum47UtMCkbXADSgvOCBZUiKLkVJNWYaprwSkD1YKApqGa1hVJpyhpcZa9O3h3YzOorlU2zd3LnTcD-Ek6MPLvjjVbuXF7yVlV17hMgldHgXc_RhWiHExoVd-DVW4MkpYJZCytOqEv_0Gv3ehtGm-mSDJyzu6pDfRKGqvd_BOzVC4qwXHNq6JO1JsD1XoXglf67skEyzkTcs6EPGYi4a8P-NbYDm7M_-gXB1olRmm4pwmrqWDFL1iFv8M</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2451756775</pqid></control><display><type>article</type><title>Houttuynia cordata Extract Ameliorates Bladder Damage and Improves Bladder Symptoms via Anti-Inflammatory Effect in Rats with Interstitial Cystitis</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central Open Access</source><source>Wiley-Blackwell Open Access Titles</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Zhou, Xiangfu ; Cai, Jiarong ; Liu, Bolong ; Zhan, Hailun ; Yang, Fei ; Li, Wenbiao ; Luo, Yun</creator><contributor>Chen, Xiaojia ; Xiaojia Chen</contributor><creatorcontrib>Zhou, Xiangfu ; Cai, Jiarong ; Liu, Bolong ; Zhan, Hailun ; Yang, Fei ; Li, Wenbiao ; Luo, Yun ; Chen, Xiaojia ; Xiaojia Chen</creatorcontrib><description>The mechanism of interstitial cystitis/bladder pain syndrome (IC/BPS) remains unclear to date, but reports showed that bladder inflammation and increasing number of activating mast cells in bladder tissues were common in patients with IC/BPS. Houttuynia cordata is widely used in Chinese traditional medicine, and its function of anti-inflammation has been proved. The purpose of this study was to investigate the efficacy and possible mechanisms of the Houttuynia cordata (HC) extract in the treatment of interstitial cystitis/bladder pain syndrome (IC/BPS). In the current study, a total of 30 adult female rats were randomly divided into three groups: sham group (n = 10), cyclophosphamide + saline (CYP + NS) group (n = 10), and cyclophosphamide + Houttuynia cordata extract (CYP + HC) group (n = 10). The animal model of IC/BPS was induced with cyclophosphamide (75 mg/kg, intraperitoneal injection, once every 3 days for 10 days) in the CYP + NS group and CYP + HC group, and sham rats received a volume-matched injection of saline. After anesthesia with urethane (0.8 g/kg, intraperitoneal injection), intravesical administration of either saline (1 ml) or Houttuynia cordata extract (1 ml, 2 g/ml) was continued once per day for a week in the CYP + NS group and CYP + HC group, respectively. Subsequently, urinary frequency, nociceptive behaviors, cystometry, bladder weight, histological changes, and cytokine (IL-6, IL-8, TNF-α) concentration were evaluated and compared among the three groups. Variables including inflammatory grade, mast cell number, proportion of activated mast cells, bladder weight, cytokine concentration of bladder homogenates, and frequency of urination significantly increased in the CYP + NS group compared with the sham group (P<0.01) and CYP + HC group (P<0.01). Besides, compared with the CYP + NS group, longer intercontraction interval, bigger bladder capacity, higher nociceptive threshold, fewer number of mast cells, and lower proportion of activated mast cells were found in the CYP + HC group (P<0.01). Our study demonstrated that the Houttuynia cordata extract can effectively inhibit mast cell proliferation and activation and downregulate proinflammatory cytokine in a rat model of IC/BPS induced with cyclophosphamide and might be potentially valuable for the treatment of IC/BPS.</description><identifier>ISSN: 1741-427X</identifier><identifier>EISSN: 1741-4288</identifier><identifier>DOI: 10.1155/2020/9026901</identifier><identifier>PMID: 33133219</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Anesthesia ; Animal models ; Anti-inflammatory agents ; Anti-inflammatory drugs ; Bladder ; Catheters ; Cell activation ; Cell number ; Cell proliferation ; Cyclophosphamide ; Cystitis ; Cytochrome P-450 ; Cytokines ; Drug therapy ; Edema ; Ethyl carbamate ; Hemorrhage ; Histopathology ; Houttuynia cordata ; Inflammation ; Injection ; Interleukin 6 ; Interleukin 8 ; Mast cells ; Methylene blue ; Pain ; Pain perception ; Rodents ; Statistical analysis ; Tumor necrosis factor-TNF ; Tumor necrosis factor-α ; Urination</subject><ispartof>Evidence-based complementary and alternative medicine, 2020, Vol.2020 (2020), p.1-7</ispartof><rights>Copyright © 2020 Wenbiao Li et al.</rights><rights>COPYRIGHT 2020 John Wiley & Sons, Inc.</rights><rights>Copyright © 2020 Wenbiao Li et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2020 Wenbiao Li et al. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c476t-5a04bd182301cb0baef9751092993472f1ce02f89725aeca9abb2f28611113423</citedby><cites>FETCH-LOGICAL-c476t-5a04bd182301cb0baef9751092993472f1ce02f89725aeca9abb2f28611113423</cites><orcidid>0000-0002-7223-6691</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7568804/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7568804/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,4009,27902,27903,27904,53769,53771</link.rule.ids></links><search><contributor>Chen, Xiaojia</contributor><contributor>Xiaojia Chen</contributor><creatorcontrib>Zhou, Xiangfu</creatorcontrib><creatorcontrib>Cai, Jiarong</creatorcontrib><creatorcontrib>Liu, Bolong</creatorcontrib><creatorcontrib>Zhan, Hailun</creatorcontrib><creatorcontrib>Yang, Fei</creatorcontrib><creatorcontrib>Li, Wenbiao</creatorcontrib><creatorcontrib>Luo, Yun</creatorcontrib><title>Houttuynia cordata Extract Ameliorates Bladder Damage and Improves Bladder Symptoms via Anti-Inflammatory Effect in Rats with Interstitial Cystitis</title><title>Evidence-based complementary and alternative medicine</title><description>The mechanism of interstitial cystitis/bladder pain syndrome (IC/BPS) remains unclear to date, but reports showed that bladder inflammation and increasing number of activating mast cells in bladder tissues were common in patients with IC/BPS. Houttuynia cordata is widely used in Chinese traditional medicine, and its function of anti-inflammation has been proved. The purpose of this study was to investigate the efficacy and possible mechanisms of the Houttuynia cordata (HC) extract in the treatment of interstitial cystitis/bladder pain syndrome (IC/BPS). In the current study, a total of 30 adult female rats were randomly divided into three groups: sham group (n = 10), cyclophosphamide + saline (CYP + NS) group (n = 10), and cyclophosphamide + Houttuynia cordata extract (CYP + HC) group (n = 10). The animal model of IC/BPS was induced with cyclophosphamide (75 mg/kg, intraperitoneal injection, once every 3 days for 10 days) in the CYP + NS group and CYP + HC group, and sham rats received a volume-matched injection of saline. After anesthesia with urethane (0.8 g/kg, intraperitoneal injection), intravesical administration of either saline (1 ml) or Houttuynia cordata extract (1 ml, 2 g/ml) was continued once per day for a week in the CYP + NS group and CYP + HC group, respectively. Subsequently, urinary frequency, nociceptive behaviors, cystometry, bladder weight, histological changes, and cytokine (IL-6, IL-8, TNF-α) concentration were evaluated and compared among the three groups. Variables including inflammatory grade, mast cell number, proportion of activated mast cells, bladder weight, cytokine concentration of bladder homogenates, and frequency of urination significantly increased in the CYP + NS group compared with the sham group (P<0.01) and CYP + HC group (P<0.01). Besides, compared with the CYP + NS group, longer intercontraction interval, bigger bladder capacity, higher nociceptive threshold, fewer number of mast cells, and lower proportion of activated mast cells were found in the CYP + HC group (P<0.01). Our study demonstrated that the Houttuynia cordata extract can effectively inhibit mast cell proliferation and activation and downregulate proinflammatory cytokine in a rat model of IC/BPS induced with cyclophosphamide and might be potentially valuable for the treatment of IC/BPS.</description><subject>Anesthesia</subject><subject>Animal models</subject><subject>Anti-inflammatory agents</subject><subject>Anti-inflammatory drugs</subject><subject>Bladder</subject><subject>Catheters</subject><subject>Cell activation</subject><subject>Cell number</subject><subject>Cell proliferation</subject><subject>Cyclophosphamide</subject><subject>Cystitis</subject><subject>Cytochrome P-450</subject><subject>Cytokines</subject><subject>Drug therapy</subject><subject>Edema</subject><subject>Ethyl carbamate</subject><subject>Hemorrhage</subject><subject>Histopathology</subject><subject>Houttuynia cordata</subject><subject>Inflammation</subject><subject>Injection</subject><subject>Interleukin 6</subject><subject>Interleukin 8</subject><subject>Mast cells</subject><subject>Methylene blue</subject><subject>Pain</subject><subject>Pain perception</subject><subject>Rodents</subject><subject>Statistical analysis</subject><subject>Tumor necrosis factor-TNF</subject><subject>Tumor necrosis 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cordata Extract Ameliorates Bladder Damage and Improves Bladder Symptoms via Anti-Inflammatory Effect in Rats with Interstitial Cystitis</title><author>Zhou, Xiangfu ; Cai, Jiarong ; Liu, Bolong ; Zhan, Hailun ; Yang, Fei ; Li, Wenbiao ; Luo, Yun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c476t-5a04bd182301cb0baef9751092993472f1ce02f89725aeca9abb2f28611113423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Anesthesia</topic><topic>Animal models</topic><topic>Anti-inflammatory agents</topic><topic>Anti-inflammatory drugs</topic><topic>Bladder</topic><topic>Catheters</topic><topic>Cell activation</topic><topic>Cell number</topic><topic>Cell proliferation</topic><topic>Cyclophosphamide</topic><topic>Cystitis</topic><topic>Cytochrome P-450</topic><topic>Cytokines</topic><topic>Drug therapy</topic><topic>Edema</topic><topic>Ethyl 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Effect in Rats with Interstitial Cystitis</atitle><jtitle>Evidence-based complementary and alternative medicine</jtitle><date>2020</date><risdate>2020</risdate><volume>2020</volume><issue>2020</issue><spage>1</spage><epage>7</epage><pages>1-7</pages><issn>1741-427X</issn><eissn>1741-4288</eissn><abstract>The mechanism of interstitial cystitis/bladder pain syndrome (IC/BPS) remains unclear to date, but reports showed that bladder inflammation and increasing number of activating mast cells in bladder tissues were common in patients with IC/BPS. Houttuynia cordata is widely used in Chinese traditional medicine, and its function of anti-inflammation has been proved. The purpose of this study was to investigate the efficacy and possible mechanisms of the Houttuynia cordata (HC) extract in the treatment of interstitial cystitis/bladder pain syndrome (IC/BPS). In the current study, a total of 30 adult female rats were randomly divided into three groups: sham group (n = 10), cyclophosphamide + saline (CYP + NS) group (n = 10), and cyclophosphamide + Houttuynia cordata extract (CYP + HC) group (n = 10). The animal model of IC/BPS was induced with cyclophosphamide (75 mg/kg, intraperitoneal injection, once every 3 days for 10 days) in the CYP + NS group and CYP + HC group, and sham rats received a volume-matched injection of saline. After anesthesia with urethane (0.8 g/kg, intraperitoneal injection), intravesical administration of either saline (1 ml) or Houttuynia cordata extract (1 ml, 2 g/ml) was continued once per day for a week in the CYP + NS group and CYP + HC group, respectively. Subsequently, urinary frequency, nociceptive behaviors, cystometry, bladder weight, histological changes, and cytokine (IL-6, IL-8, TNF-α) concentration were evaluated and compared among the three groups. Variables including inflammatory grade, mast cell number, proportion of activated mast cells, bladder weight, cytokine concentration of bladder homogenates, and frequency of urination significantly increased in the CYP + NS group compared with the sham group (P<0.01) and CYP + HC group (P<0.01). Besides, compared with the CYP + NS group, longer intercontraction interval, bigger bladder capacity, higher nociceptive threshold, fewer number of mast cells, and lower proportion of activated mast cells were found in the CYP + HC group (P<0.01). Our study demonstrated that the Houttuynia cordata extract can effectively inhibit mast cell proliferation and activation and downregulate proinflammatory cytokine in a rat model of IC/BPS induced with cyclophosphamide and might be potentially valuable for the treatment of IC/BPS.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>33133219</pmid><doi>10.1155/2020/9026901</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-7223-6691</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; Wiley-Blackwell Open Access Titles; PubMed Central; Alma/SFX Local Collection |
| subjects | Anesthesia Animal models Anti-inflammatory agents Anti-inflammatory drugs Bladder Catheters Cell activation Cell number Cell proliferation Cyclophosphamide Cystitis Cytochrome P-450 Cytokines Drug therapy Edema Ethyl carbamate Hemorrhage Histopathology Houttuynia cordata Inflammation Injection Interleukin 6 Interleukin 8 Mast cells Methylene blue Pain Pain perception Rodents Statistical analysis Tumor necrosis factor-TNF Tumor necrosis factor-α Urination |
| title | Houttuynia cordata Extract Ameliorates Bladder Damage and Improves Bladder Symptoms via Anti-Inflammatory Effect in Rats with Interstitial Cystitis |
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