Risk of genotoxic damage in schoolchildren exposed to organochloride pesticides
This study identified and determined organochloride pesticide (OCs) concentrations in hair samples from children at two elementary schools: one exposed to fumigations in agricultural fields, the other unexposed. Three concentrations of OCs levels in the hair were compared (high, medium, low), and to...
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| Veröffentlicht in: | Scientific reports 2020-10, Vol.10 (1), p.17584, Article 17584 |
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| description | This study identified and determined organochloride pesticide (OCs) concentrations in hair samples from children at two elementary schools: one exposed to fumigations in agricultural fields, the other unexposed. Three concentrations of OCs levels in the hair were compared (high, medium, low), and total nuclear abnormalities in buccal cells were determined: micronuclei (MNi), condensed chromatin, karyorrhexis, pyknosis, binucleate cells, karyolysis, lobed nuclei, and apoptosis. No significant differences were found for the presence of MNi between the schoolchildren from the exposed and unexposed schools, but the prevalence of OCs in both schools was over 50%, as well as the frequencies of MNi in the children were over 58%. Findings show a significant difference between the frequency of MNi in the total sample of schoolchildren (exposed school + unexposed school) in relation to the concentration of OCs detected in their hair. The children from exposed school that showed the higher concentrations of OCs in hair had higher levels of genotoxic damage in the buccal cells; compared against children with lower concentrations of OCs. The most frequent nuclear abnormalities in the exposed children were lobed nuclei (79.4%), binucleate cells (66.66%), apoptosis (65.07), and MNi (58.7%). We determined the prevalence ratio (PR) and prevalence odds ratio (POR) for the presence of MNi in buccal cells in relation to the OCs concentrations in the hair samples. Both ratios were high for MNi [PR 3.93, 95% confidence interval (CI) 1.97–7.84,
p
= 0.0003; and POR 7.97, 95% CI 2.62–24.28,
p
= 0.0003], indicating a 7.97 times greater risk that the exposed children will present > 0.2% of MNi when OCs concentrations exceed 0.447 μg/g. These indicators may be useful biomarkers of genotoxic damage in children exposed to persistent, highly-toxic compounds. Results suggest the potential risk to which those schoolchildren are exposed on a daily basis due to fumigations in nearby agricultural fields. |
| doi_str_mv | 10.1038/s41598-020-74620-w |
| format | Article |
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p
= 0.0003; and POR 7.97, 95% CI 2.62–24.28,
p
= 0.0003], indicating a 7.97 times greater risk that the exposed children will present > 0.2% of MNi when OCs concentrations exceed 0.447 μg/g. These indicators may be useful biomarkers of genotoxic damage in children exposed to persistent, highly-toxic compounds. Results suggest the potential risk to which those schoolchildren are exposed on a daily basis due to fumigations in nearby agricultural fields.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-020-74620-w</identifier><identifier>PMID: 33067503</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/80 ; 692/499 ; 704/172 ; Cell Death ; Cell Nucleus ; Child ; Chromatography, Gas - methods ; DNA Damage ; Environmental Exposure - adverse effects ; Environmental Exposure - analysis ; Female ; Hair - chemistry ; Hair - metabolism ; Humanities and Social Sciences ; Humans ; Hydrocarbons, Chlorinated - adverse effects ; Hydrocarbons, Chlorinated - toxicity ; Male ; Micronuclei, Chromosome-Defective - statistics & numerical data ; Micronucleus Tests - methods ; Mouth Mucosa - chemistry ; Mouth Mucosa - metabolism ; multidisciplinary ; Pesticides - adverse effects ; Pesticides - toxicity ; Science ; Science (multidisciplinary)</subject><ispartof>Scientific reports, 2020-10, Vol.10 (1), p.17584, Article 17584</ispartof><rights>The Author(s) 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-2e5e9eaafdf6ec5fbc2b397f6520f35f9ae91f0152ea03bfe34bfbf9a84775e63</citedby><cites>FETCH-LOGICAL-c499t-2e5e9eaafdf6ec5fbc2b397f6520f35f9ae91f0152ea03bfe34bfbf9a84775e63</cites><orcidid>0000-0002-3784-7358</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7567889/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7567889/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,41096,42165,51551,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33067503$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Anguiano-Vega, Gerardo Alfonso</creatorcontrib><creatorcontrib>Cazares-Ramirez, Linette Hazel</creatorcontrib><creatorcontrib>Rendon-Von Osten, Jaime</creatorcontrib><creatorcontrib>Santillan-Sidon, Alma Patricia</creatorcontrib><creatorcontrib>Vazquez-Boucard, Celia Gloria</creatorcontrib><title>Risk of genotoxic damage in schoolchildren exposed to organochloride pesticides</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>This study identified and determined organochloride pesticide (OCs) concentrations in hair samples from children at two elementary schools: one exposed to fumigations in agricultural fields, the other unexposed. Three concentrations of OCs levels in the hair were compared (high, medium, low), and total nuclear abnormalities in buccal cells were determined: micronuclei (MNi), condensed chromatin, karyorrhexis, pyknosis, binucleate cells, karyolysis, lobed nuclei, and apoptosis. No significant differences were found for the presence of MNi between the schoolchildren from the exposed and unexposed schools, but the prevalence of OCs in both schools was over 50%, as well as the frequencies of MNi in the children were over 58%. Findings show a significant difference between the frequency of MNi in the total sample of schoolchildren (exposed school + unexposed school) in relation to the concentration of OCs detected in their hair. The children from exposed school that showed the higher concentrations of OCs in hair had higher levels of genotoxic damage in the buccal cells; compared against children with lower concentrations of OCs. The most frequent nuclear abnormalities in the exposed children were lobed nuclei (79.4%), binucleate cells (66.66%), apoptosis (65.07), and MNi (58.7%). We determined the prevalence ratio (PR) and prevalence odds ratio (POR) for the presence of MNi in buccal cells in relation to the OCs concentrations in the hair samples. Both ratios were high for MNi [PR 3.93, 95% confidence interval (CI) 1.97–7.84,
p
= 0.0003; and POR 7.97, 95% CI 2.62–24.28,
p
= 0.0003], indicating a 7.97 times greater risk that the exposed children will present > 0.2% of MNi when OCs concentrations exceed 0.447 μg/g. These indicators may be useful biomarkers of genotoxic damage in children exposed to persistent, highly-toxic compounds. Results suggest the potential risk to which those schoolchildren are exposed on a daily basis due to fumigations in nearby agricultural fields.</description><subject>631/80</subject><subject>692/499</subject><subject>704/172</subject><subject>Cell Death</subject><subject>Cell Nucleus</subject><subject>Child</subject><subject>Chromatography, Gas - methods</subject><subject>DNA Damage</subject><subject>Environmental Exposure - adverse effects</subject><subject>Environmental Exposure - analysis</subject><subject>Female</subject><subject>Hair - chemistry</subject><subject>Hair - metabolism</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Hydrocarbons, Chlorinated - adverse effects</subject><subject>Hydrocarbons, Chlorinated - toxicity</subject><subject>Male</subject><subject>Micronuclei, Chromosome-Defective - statistics & numerical data</subject><subject>Micronucleus Tests - methods</subject><subject>Mouth Mucosa - chemistry</subject><subject>Mouth Mucosa - metabolism</subject><subject>multidisciplinary</subject><subject>Pesticides - adverse effects</subject><subject>Pesticides - toxicity</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><recordid>eNp9kN9LwzAQx4Mobsz9Az5I_oFqmjRt8yLI8BcIA9HnkKaXLrNrRtK5-d8brY754j3cHXf3_R58EDpPyWVKWHkVspSLMiGUJEWWx7w9QmNKMp5QRunxQT9C0xCWJAanIkvFKRoxRvKCEzZG82cb3rAzuIHO9W5nNa7VSjWAbYeDXjjX6oVtaw8dht3aBahx77DzjeqcXrTO2xrwGkJvdezCGToxqg0w_akT9Hp3-zJ7SJ7m94-zm6dEZ0L0CQUOApQytclBc1NpWjFRmJxTYhg3QoFIDUk5BUVYZYBllaniuMyKgkPOJuh68F1vqhXUGrreq1auvV0p_yGdsvLvprML2bh3WfC8KEsRDehgoL0LwYPZa1MivwjLgbCMhOU3YbmNoovDr3vJL894wIaDEFddA14u3cZ3kcR_tp9haIsu</recordid><startdate>20201016</startdate><enddate>20201016</enddate><creator>Anguiano-Vega, Gerardo Alfonso</creator><creator>Cazares-Ramirez, Linette Hazel</creator><creator>Rendon-Von Osten, Jaime</creator><creator>Santillan-Sidon, Alma Patricia</creator><creator>Vazquez-Boucard, Celia Gloria</creator><general>Nature Publishing Group UK</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-3784-7358</orcidid></search><sort><creationdate>20201016</creationdate><title>Risk of genotoxic damage in schoolchildren exposed to organochloride pesticides</title><author>Anguiano-Vega, Gerardo Alfonso ; Cazares-Ramirez, Linette Hazel ; Rendon-Von Osten, Jaime ; Santillan-Sidon, Alma Patricia ; Vazquez-Boucard, Celia Gloria</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-2e5e9eaafdf6ec5fbc2b397f6520f35f9ae91f0152ea03bfe34bfbf9a84775e63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>631/80</topic><topic>692/499</topic><topic>704/172</topic><topic>Cell Death</topic><topic>Cell Nucleus</topic><topic>Child</topic><topic>Chromatography, Gas - methods</topic><topic>DNA Damage</topic><topic>Environmental Exposure - adverse effects</topic><topic>Environmental Exposure - analysis</topic><topic>Female</topic><topic>Hair - chemistry</topic><topic>Hair - metabolism</topic><topic>Humanities and Social Sciences</topic><topic>Humans</topic><topic>Hydrocarbons, Chlorinated - adverse effects</topic><topic>Hydrocarbons, Chlorinated - toxicity</topic><topic>Male</topic><topic>Micronuclei, Chromosome-Defective - statistics & numerical data</topic><topic>Micronucleus Tests - methods</topic><topic>Mouth Mucosa - chemistry</topic><topic>Mouth Mucosa - metabolism</topic><topic>multidisciplinary</topic><topic>Pesticides - adverse effects</topic><topic>Pesticides - toxicity</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Anguiano-Vega, Gerardo Alfonso</creatorcontrib><creatorcontrib>Cazares-Ramirez, Linette Hazel</creatorcontrib><creatorcontrib>Rendon-Von Osten, Jaime</creatorcontrib><creatorcontrib>Santillan-Sidon, Alma Patricia</creatorcontrib><creatorcontrib>Vazquez-Boucard, Celia Gloria</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Anguiano-Vega, Gerardo Alfonso</au><au>Cazares-Ramirez, Linette Hazel</au><au>Rendon-Von Osten, Jaime</au><au>Santillan-Sidon, Alma Patricia</au><au>Vazquez-Boucard, Celia Gloria</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Risk of genotoxic damage in schoolchildren exposed to organochloride pesticides</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2020-10-16</date><risdate>2020</risdate><volume>10</volume><issue>1</issue><spage>17584</spage><pages>17584-</pages><artnum>17584</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>This study identified and determined organochloride pesticide (OCs) concentrations in hair samples from children at two elementary schools: one exposed to fumigations in agricultural fields, the other unexposed. Three concentrations of OCs levels in the hair were compared (high, medium, low), and total nuclear abnormalities in buccal cells were determined: micronuclei (MNi), condensed chromatin, karyorrhexis, pyknosis, binucleate cells, karyolysis, lobed nuclei, and apoptosis. No significant differences were found for the presence of MNi between the schoolchildren from the exposed and unexposed schools, but the prevalence of OCs in both schools was over 50%, as well as the frequencies of MNi in the children were over 58%. Findings show a significant difference between the frequency of MNi in the total sample of schoolchildren (exposed school + unexposed school) in relation to the concentration of OCs detected in their hair. The children from exposed school that showed the higher concentrations of OCs in hair had higher levels of genotoxic damage in the buccal cells; compared against children with lower concentrations of OCs. The most frequent nuclear abnormalities in the exposed children were lobed nuclei (79.4%), binucleate cells (66.66%), apoptosis (65.07), and MNi (58.7%). We determined the prevalence ratio (PR) and prevalence odds ratio (POR) for the presence of MNi in buccal cells in relation to the OCs concentrations in the hair samples. Both ratios were high for MNi [PR 3.93, 95% confidence interval (CI) 1.97–7.84,
p
= 0.0003; and POR 7.97, 95% CI 2.62–24.28,
p
= 0.0003], indicating a 7.97 times greater risk that the exposed children will present > 0.2% of MNi when OCs concentrations exceed 0.447 μg/g. These indicators may be useful biomarkers of genotoxic damage in children exposed to persistent, highly-toxic compounds. Results suggest the potential risk to which those schoolchildren are exposed on a daily basis due to fumigations in nearby agricultural fields.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>33067503</pmid><doi>10.1038/s41598-020-74620-w</doi><orcidid>https://orcid.org/0000-0002-3784-7358</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 631/80 692/499 704/172 Cell Death Cell Nucleus Child Chromatography, Gas - methods DNA Damage Environmental Exposure - adverse effects Environmental Exposure - analysis Female Hair - chemistry Hair - metabolism Humanities and Social Sciences Humans Hydrocarbons, Chlorinated - adverse effects Hydrocarbons, Chlorinated - toxicity Male Micronuclei, Chromosome-Defective - statistics & numerical data Micronucleus Tests - methods Mouth Mucosa - chemistry Mouth Mucosa - metabolism multidisciplinary Pesticides - adverse effects Pesticides - toxicity Science Science (multidisciplinary) |
| title | Risk of genotoxic damage in schoolchildren exposed to organochloride pesticides |
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