Phylogenetic analysis of infectious bronchitis virus circulating in southern China in 2016–2017 and evaluation of an attenuated strain as a vaccine candidate
Avian infectious bronchitis (IB) is a highly contagious viral respiratory disease, caused by infectious bronchitis virus (IBV), that poses an important economic threat to the poultry industry. In recent years, genotypes GI-7, GI-13, and GI-19 have been the most prevalent IBV strains in China. Howeve...
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| Veröffentlicht in: | Archives of virology 2021-01, Vol.166 (1), p.73-81 |
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| creator | Chen, Libin Xiang, Bin Hong, Yanfen Li, Qian Du, Haoyun Lin, Qiuyan Liao, Ming Ren, Tao Xu, Chenggang |
| description | Avian infectious bronchitis (IB) is a highly contagious viral respiratory disease, caused by infectious bronchitis virus (IBV), that poses an important economic threat to the poultry industry. In recent years, genotypes GI-7, GI-13, and GI-19 have been the most prevalent IBV strains in China. However, in this study, we found that most IBV strains from southern China in 2016–2017 belonged to genotype GVI-1. This genotype, for which there is no vaccine, has been reported sporadically in the region. The GDTS13 strain, which caused severe IB outbreaks on the farms where it was isolated, was evaluated as a candidate vaccine strain. GDTS13 was serially passaged in specific-pathogen-free embryonated chicken eggs for 100 generations to produce GDTS13-F100. Safety testing indicated that GDTS13-F100 had no pathogenic effect on chickens. Additionally, GDTS13-F100 showed an excellent protective effect against GDTS13, with no clinical signs or virus shedding observed in immunized chickens challenged with the parent strain. These findings indicate that GVI-1 has become the most prevalent IBV genotype in southern China and that GDTS13-F100 may serve as an attenuated vaccine to protect against infection with this genotype. |
| doi_str_mv | 10.1007/s00705-020-04851-9 |
| format | Article |
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In recent years, genotypes GI-7, GI-13, and GI-19 have been the most prevalent IBV strains in China. However, in this study, we found that most IBV strains from southern China in 2016–2017 belonged to genotype GVI-1. This genotype, for which there is no vaccine, has been reported sporadically in the region. The GDTS13 strain, which caused severe IB outbreaks on the farms where it was isolated, was evaluated as a candidate vaccine strain. GDTS13 was serially passaged in specific-pathogen-free embryonated chicken eggs for 100 generations to produce GDTS13-F100. Safety testing indicated that GDTS13-F100 had no pathogenic effect on chickens. Additionally, GDTS13-F100 showed an excellent protective effect against GDTS13, with no clinical signs or virus shedding observed in immunized chickens challenged with the parent strain. These findings indicate that GVI-1 has become the most prevalent IBV genotype in southern China and that GDTS13-F100 may serve as an attenuated vaccine to protect against infection with this genotype.</description><identifier>ISSN: 0304-8608</identifier><identifier>EISSN: 1432-8798</identifier><identifier>DOI: 10.1007/s00705-020-04851-9</identifier><identifier>PMID: 33067648</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Animals ; Avian infectious bronchitis ; Biomedical and Life Sciences ; Biomedicine ; Bronchitis ; Chickens - virology ; China ; Coronavirus Infections - immunology ; Coronavirus Infections - virology ; Eggs ; Farms ; Genotype ; Genotype & phenotype ; Genotypes ; Infectious bronchitis virus - genetics ; Infectious bronchitis virus - immunology ; Infectious Diseases ; Medical Microbiology ; Original ; Original Article ; Phylogeny ; Poultry Diseases - immunology ; Poultry Diseases - virology ; Respiratory diseases ; Specific Pathogen-Free Organisms - genetics ; Strains (organisms) ; Vaccination - methods ; Vaccines ; Vaccines, Attenuated - immunology ; Viral Vaccines - immunology ; Virology</subject><ispartof>Archives of virology, 2021-01, Vol.166 (1), p.73-81</ispartof><rights>Springer-Verlag GmbH Austria, part of Springer Nature 2020</rights><rights>Springer-Verlag GmbH Austria, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-f375c341d1d39c3e123e3e6ed693416adb85f45a0bd6f82668e8da7a372f44233</citedby><cites>FETCH-LOGICAL-c474t-f375c341d1d39c3e123e3e6ed693416adb85f45a0bd6f82668e8da7a372f44233</cites><orcidid>0000-0003-4146-9895</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00705-020-04851-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00705-020-04851-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,780,784,885,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33067648$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Libin</creatorcontrib><creatorcontrib>Xiang, Bin</creatorcontrib><creatorcontrib>Hong, Yanfen</creatorcontrib><creatorcontrib>Li, Qian</creatorcontrib><creatorcontrib>Du, Haoyun</creatorcontrib><creatorcontrib>Lin, Qiuyan</creatorcontrib><creatorcontrib>Liao, Ming</creatorcontrib><creatorcontrib>Ren, Tao</creatorcontrib><creatorcontrib>Xu, Chenggang</creatorcontrib><title>Phylogenetic analysis of infectious bronchitis virus circulating in southern China in 2016–2017 and evaluation of an attenuated strain as a vaccine candidate</title><title>Archives of virology</title><addtitle>Arch Virol</addtitle><addtitle>Arch Virol</addtitle><description>Avian infectious bronchitis (IB) is a highly contagious viral respiratory disease, caused by infectious bronchitis virus (IBV), that poses an important economic threat to the poultry industry. In recent years, genotypes GI-7, GI-13, and GI-19 have been the most prevalent IBV strains in China. However, in this study, we found that most IBV strains from southern China in 2016–2017 belonged to genotype GVI-1. This genotype, for which there is no vaccine, has been reported sporadically in the region. The GDTS13 strain, which caused severe IB outbreaks on the farms where it was isolated, was evaluated as a candidate vaccine strain. GDTS13 was serially passaged in specific-pathogen-free embryonated chicken eggs for 100 generations to produce GDTS13-F100. Safety testing indicated that GDTS13-F100 had no pathogenic effect on chickens. Additionally, GDTS13-F100 showed an excellent protective effect against GDTS13, with no clinical signs or virus shedding observed in immunized chickens challenged with the parent strain. These findings indicate that GVI-1 has become the most prevalent IBV genotype in southern China and that GDTS13-F100 may serve as an attenuated vaccine to protect against infection with this genotype.</description><subject>Animals</subject><subject>Avian infectious bronchitis</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Bronchitis</subject><subject>Chickens - virology</subject><subject>China</subject><subject>Coronavirus Infections - immunology</subject><subject>Coronavirus Infections - virology</subject><subject>Eggs</subject><subject>Farms</subject><subject>Genotype</subject><subject>Genotype & phenotype</subject><subject>Genotypes</subject><subject>Infectious bronchitis virus - genetics</subject><subject>Infectious bronchitis virus - immunology</subject><subject>Infectious Diseases</subject><subject>Medical Microbiology</subject><subject>Original</subject><subject>Original Article</subject><subject>Phylogeny</subject><subject>Poultry Diseases - immunology</subject><subject>Poultry Diseases - virology</subject><subject>Respiratory diseases</subject><subject>Specific Pathogen-Free Organisms - genetics</subject><subject>Strains (organisms)</subject><subject>Vaccination - methods</subject><subject>Vaccines</subject><subject>Vaccines, Attenuated - immunology</subject><subject>Viral Vaccines - immunology</subject><subject>Virology</subject><issn>0304-8608</issn><issn>1432-8798</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9UcuKFDEULURx2tEfcCEBN25K86okvRGk8QUDutB1SKdudWWoTsYk1dC7-Qc_wH_zS7xlj-Nj4SaB88rNPU3zmNHnjFL9ouBBu5Zy2lJpOtau7zQrJgVvjV6bu82KCipbo6g5ax6UckkpAqK735wJQZVW0qyabx_H45R2EKEGT1x007GEQtJAQhzA15DmQrY5RT-GisQhZAR8yH6eXA1xhzpS0lxHyJFsxhDdgnDK1Pfrr3hpDO0JHNw0oz7FJdpF4mqFiAj0pNTs0OIKceTgvA8RiEdT6JF-2Nwb3FTg0c193nx-8_rT5l178eHt-82ri9ZLLWs7CN15IVnPerH2AhgXIEBBr9aIKtdvTTfIztFtrwbDlTJgeqed0HyQkgtx3rw85V7N2z30HiJONdmrHPYuH21ywf7NxDDaXTpY3SnVGYYBz24CcvoyQ6l2H4qHaXIRcIeWy44ZxY3oUPr0H-llmjOuflFpY5SRYgnkJ5XPqZQMw-0wjNqlf3vq32L_9mf_do2mJ39-49byq3AUiJOgIBV3kH-__Z_YH2j-vr8</recordid><startdate>20210101</startdate><enddate>20210101</enddate><creator>Chen, Libin</creator><creator>Xiang, Bin</creator><creator>Hong, Yanfen</creator><creator>Li, Qian</creator><creator>Du, Haoyun</creator><creator>Lin, Qiuyan</creator><creator>Liao, Ming</creator><creator>Ren, Tao</creator><creator>Xu, Chenggang</creator><general>Springer Vienna</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-4146-9895</orcidid></search><sort><creationdate>20210101</creationdate><title>Phylogenetic analysis of infectious bronchitis virus circulating in southern China in 2016–2017 and evaluation of an attenuated strain as a vaccine candidate</title><author>Chen, Libin ; Xiang, Bin ; Hong, Yanfen ; Li, Qian ; Du, Haoyun ; Lin, Qiuyan ; Liao, Ming ; Ren, Tao ; Xu, Chenggang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-f375c341d1d39c3e123e3e6ed693416adb85f45a0bd6f82668e8da7a372f44233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>Avian infectious bronchitis</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Bronchitis</topic><topic>Chickens - virology</topic><topic>China</topic><topic>Coronavirus Infections - immunology</topic><topic>Coronavirus Infections - virology</topic><topic>Eggs</topic><topic>Farms</topic><topic>Genotype</topic><topic>Genotype & phenotype</topic><topic>Genotypes</topic><topic>Infectious bronchitis virus - genetics</topic><topic>Infectious bronchitis virus - immunology</topic><topic>Infectious Diseases</topic><topic>Medical Microbiology</topic><topic>Original</topic><topic>Original Article</topic><topic>Phylogeny</topic><topic>Poultry Diseases - immunology</topic><topic>Poultry Diseases - virology</topic><topic>Respiratory diseases</topic><topic>Specific Pathogen-Free Organisms - genetics</topic><topic>Strains (organisms)</topic><topic>Vaccination - methods</topic><topic>Vaccines</topic><topic>Vaccines, Attenuated - immunology</topic><topic>Viral Vaccines - immunology</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Libin</creatorcontrib><creatorcontrib>Xiang, Bin</creatorcontrib><creatorcontrib>Hong, Yanfen</creatorcontrib><creatorcontrib>Li, Qian</creatorcontrib><creatorcontrib>Du, Haoyun</creatorcontrib><creatorcontrib>Lin, Qiuyan</creatorcontrib><creatorcontrib>Liao, Ming</creatorcontrib><creatorcontrib>Ren, Tao</creatorcontrib><creatorcontrib>Xu, Chenggang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Archives of virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Libin</au><au>Xiang, Bin</au><au>Hong, Yanfen</au><au>Li, Qian</au><au>Du, Haoyun</au><au>Lin, Qiuyan</au><au>Liao, Ming</au><au>Ren, Tao</au><au>Xu, Chenggang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phylogenetic analysis of infectious bronchitis virus circulating in southern China in 2016–2017 and evaluation of an attenuated strain as a vaccine candidate</atitle><jtitle>Archives of virology</jtitle><stitle>Arch Virol</stitle><addtitle>Arch Virol</addtitle><date>2021-01-01</date><risdate>2021</risdate><volume>166</volume><issue>1</issue><spage>73</spage><epage>81</epage><pages>73-81</pages><issn>0304-8608</issn><eissn>1432-8798</eissn><abstract>Avian infectious bronchitis (IB) is a highly contagious viral respiratory disease, caused by infectious bronchitis virus (IBV), that poses an important economic threat to the poultry industry. In recent years, genotypes GI-7, GI-13, and GI-19 have been the most prevalent IBV strains in China. However, in this study, we found that most IBV strains from southern China in 2016–2017 belonged to genotype GVI-1. This genotype, for which there is no vaccine, has been reported sporadically in the region. The GDTS13 strain, which caused severe IB outbreaks on the farms where it was isolated, was evaluated as a candidate vaccine strain. GDTS13 was serially passaged in specific-pathogen-free embryonated chicken eggs for 100 generations to produce GDTS13-F100. Safety testing indicated that GDTS13-F100 had no pathogenic effect on chickens. Additionally, GDTS13-F100 showed an excellent protective effect against GDTS13, with no clinical signs or virus shedding observed in immunized chickens challenged with the parent strain. These findings indicate that GVI-1 has become the most prevalent IBV genotype in southern China and that GDTS13-F100 may serve as an attenuated vaccine to protect against infection with this genotype.</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><pmid>33067648</pmid><doi>10.1007/s00705-020-04851-9</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0003-4146-9895</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Avian infectious bronchitis Biomedical and Life Sciences Biomedicine Bronchitis Chickens - virology China Coronavirus Infections - immunology Coronavirus Infections - virology Eggs Farms Genotype Genotype & phenotype Genotypes Infectious bronchitis virus - genetics Infectious bronchitis virus - immunology Infectious Diseases Medical Microbiology Original Original Article Phylogeny Poultry Diseases - immunology Poultry Diseases - virology Respiratory diseases Specific Pathogen-Free Organisms - genetics Strains (organisms) Vaccination - methods Vaccines Vaccines, Attenuated - immunology Viral Vaccines - immunology Virology |
| title | Phylogenetic analysis of infectious bronchitis virus circulating in southern China in 2016–2017 and evaluation of an attenuated strain as a vaccine candidate |
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