Genes Modulating the Increase in Sexuality in the Facultative Diplosporous Grass Eragrostis curvula under Water Stress Conditions
presents mainly facultative genotypes that reproduce by diplosporous apomixis, retaining a percentage of sexual pistils that increase under drought and other stressful situations, indicating that some regulators activated by stress could be affecting the apomixis/sexual switch. Water stress experime...
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| Veröffentlicht in: | Genes 2020-08, Vol.11 (9), p.969 |
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| creator | Selva, Juan Pablo Zappacosta, Diego Carballo, José Rodrigo, Juan Manuel Bellido, Andrés Gallo, Cristian Andrés Gallardo, Jimena Echenique, Viviana |
| description | presents mainly facultative genotypes that reproduce by diplosporous apomixis, retaining a percentage of sexual pistils that increase under drought and other stressful situations, indicating that some regulators activated by stress could be affecting the apomixis/sexual switch. Water stress experiments were performed in order to associate the increase in sexual embryo sacs with the differential expression of genes in a facultative apomictic cultivar using cytoembryology and RNA sequencing. The percentage of sexual embryo sacs increased from 4 to 24% and 501 out of the 201,011 transcripts were differentially expressed (DE) between control and stressed plants. DE transcripts were compared with previous transcriptomes where apomictic and sexual genotypes were contrasted. The results point as candidates to transcripts related to methylation, ubiquitination, hormone and signal transduction pathways, transcription regulation and cell wall biosynthesis, some acting as a general response to stress and some that are specific to the reproductive mode. We suggest that a DNA glycosylase EcROS1-like could be demethylating, thus de-repressing a gene or genes involved in the sexuality pathways. Many of the other DE transcripts could be part of a complex mechanism that regulates apomixis and sexuality in this grass, the ones in the intersection between control/stress and apo/sex being the strongest candidates. |
| doi_str_mv | 10.3390/genes11090969 |
| format | Article |
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Water stress experiments were performed in order to associate the increase in sexual embryo sacs with the differential expression of genes in a facultative apomictic cultivar using cytoembryology and RNA sequencing. The percentage of sexual embryo sacs increased from 4 to 24% and 501 out of the 201,011 transcripts were differentially expressed (DE) between control and stressed plants. DE transcripts were compared with previous transcriptomes where apomictic and sexual genotypes were contrasted. The results point as candidates to transcripts related to methylation, ubiquitination, hormone and signal transduction pathways, transcription regulation and cell wall biosynthesis, some acting as a general response to stress and some that are specific to the reproductive mode. We suggest that a DNA glycosylase EcROS1-like could be demethylating, thus de-repressing a gene or genes involved in the sexuality pathways. Many of the other DE transcripts could be part of a complex mechanism that regulates apomixis and sexuality in this grass, the ones in the intersection between control/stress and apo/sex being the strongest candidates.</description><identifier>ISSN: 2073-4425</identifier><identifier>EISSN: 2073-4425</identifier><identifier>DOI: 10.3390/genes11090969</identifier><identifier>PMID: 32825586</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Abiotic stress ; Apomixis ; Argentina ; Cell walls ; Cultivars ; DNA glycosylase ; DNA methylation ; Drought ; Droughts ; Environmental aspects ; Epigenetics ; Eragrostis - genetics ; Eragrostis - physiology ; Eragrostis curvula ; Gene expression ; Gene Expression Regulation, Plant ; Gene regulation ; Genetic aspects ; Genotypes ; Grasses ; MicroRNAs ; Physiological aspects ; Pistils ; Plant asexual reproduction ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Ribonucleic acid ; RNA ; Seeds ; Sequence Analysis, RNA ; Sexuality ; Signal transduction ; Stress (Physiology) ; Stress, Physiological ; Transcription ; Transcriptome ; Ubiquitination ; Water stress</subject><ispartof>Genes, 2020-08, Vol.11 (9), p.969</ispartof><rights>COPYRIGHT 2020 MDPI AG</rights><rights>2020. This work is licensed under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 by the authors. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3589-42e8dd68a5026a2464b4c14f587e9cd24d60afdaf0ba7e83b5d6e965f813869e3</citedby><cites>FETCH-LOGICAL-c3589-42e8dd68a5026a2464b4c14f587e9cd24d60afdaf0ba7e83b5d6e965f813869e3</cites><orcidid>0000-0003-3755-9062</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7564825/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7564825/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,886,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32825586$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Selva, Juan Pablo</creatorcontrib><creatorcontrib>Zappacosta, Diego</creatorcontrib><creatorcontrib>Carballo, José</creatorcontrib><creatorcontrib>Rodrigo, Juan Manuel</creatorcontrib><creatorcontrib>Bellido, Andrés</creatorcontrib><creatorcontrib>Gallo, Cristian Andrés</creatorcontrib><creatorcontrib>Gallardo, Jimena</creatorcontrib><creatorcontrib>Echenique, Viviana</creatorcontrib><title>Genes Modulating the Increase in Sexuality in the Facultative Diplosporous Grass Eragrostis curvula under Water Stress Conditions</title><title>Genes</title><addtitle>Genes (Basel)</addtitle><description>presents mainly facultative genotypes that reproduce by diplosporous apomixis, retaining a percentage of sexual pistils that increase under drought and other stressful situations, indicating that some regulators activated by stress could be affecting the apomixis/sexual switch. Water stress experiments were performed in order to associate the increase in sexual embryo sacs with the differential expression of genes in a facultative apomictic cultivar using cytoembryology and RNA sequencing. The percentage of sexual embryo sacs increased from 4 to 24% and 501 out of the 201,011 transcripts were differentially expressed (DE) between control and stressed plants. DE transcripts were compared with previous transcriptomes where apomictic and sexual genotypes were contrasted. The results point as candidates to transcripts related to methylation, ubiquitination, hormone and signal transduction pathways, transcription regulation and cell wall biosynthesis, some acting as a general response to stress and some that are specific to the reproductive mode. We suggest that a DNA glycosylase EcROS1-like could be demethylating, thus de-repressing a gene or genes involved in the sexuality pathways. Many of the other DE transcripts could be part of a complex mechanism that regulates apomixis and sexuality in this grass, the ones in the intersection between control/stress and apo/sex being the strongest candidates.</description><subject>Abiotic stress</subject><subject>Apomixis</subject><subject>Argentina</subject><subject>Cell walls</subject><subject>Cultivars</subject><subject>DNA glycosylase</subject><subject>DNA methylation</subject><subject>Drought</subject><subject>Droughts</subject><subject>Environmental aspects</subject><subject>Epigenetics</subject><subject>Eragrostis - genetics</subject><subject>Eragrostis - physiology</subject><subject>Eragrostis curvula</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>Gene regulation</subject><subject>Genetic aspects</subject><subject>Genotypes</subject><subject>Grasses</subject><subject>MicroRNAs</subject><subject>Physiological aspects</subject><subject>Pistils</subject><subject>Plant asexual reproduction</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Seeds</subject><subject>Sequence Analysis, RNA</subject><subject>Sexuality</subject><subject>Signal transduction</subject><subject>Stress (Physiology)</subject><subject>Stress, Physiological</subject><subject>Transcription</subject><subject>Transcriptome</subject><subject>Ubiquitination</subject><subject>Water stress</subject><issn>2073-4425</issn><issn>2073-4425</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdks9vFSEQx4nR2Kb26NWQePGyleXXsheT5tm-NqnxUI1HwoPZLc0-eAL7Yo_-57Jpra0cYMh88h2-wyD0tiUnjPXk4wgBctuSnvSyf4EOKelYwzkVL5_EB-g451tSFyeUEPEaHTCqqBBKHqLf60UCf4lunkzxYcTlBvBlsAlMBuwDvoZfs5l8uVsuS_Lc2HkqFd4D_ux3U8y7mOKc8TqZnPFZMmOKufiM7Zz2VRbPwUHCP0yp-3VJUKlVDM4XH0N-g14NZspw_HAeoe_nZ99WF83V1_Xl6vSqsUyovuEUlHNSGUGoNJRLvuG25YNQHfTWUe4kMYMzA9mYDhTbCCehl2JQLVOyB3aEPt3r7ubNFpyFUJKZ9C75rUl3Ohqvn2eCv9Fj3OtOSF7bVQU-PAik-HOGXPTWZwvTZAJU-5pyJlnftYxU9P1_6G2cU6j2FqqjpJWC_aNGM4H2YYi1rl1E9ankLZNEdUvZ5p6ytas5wfD45JboZQr0symo_LunPh_pv3_O_gDD26-r</recordid><startdate>20200821</startdate><enddate>20200821</enddate><creator>Selva, Juan Pablo</creator><creator>Zappacosta, Diego</creator><creator>Carballo, José</creator><creator>Rodrigo, Juan Manuel</creator><creator>Bellido, Andrés</creator><creator>Gallo, Cristian Andrés</creator><creator>Gallardo, Jimena</creator><creator>Echenique, Viviana</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-3755-9062</orcidid></search><sort><creationdate>20200821</creationdate><title>Genes Modulating the Increase in Sexuality in the Facultative Diplosporous Grass Eragrostis curvula under Water Stress Conditions</title><author>Selva, Juan Pablo ; 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Water stress experiments were performed in order to associate the increase in sexual embryo sacs with the differential expression of genes in a facultative apomictic cultivar using cytoembryology and RNA sequencing. The percentage of sexual embryo sacs increased from 4 to 24% and 501 out of the 201,011 transcripts were differentially expressed (DE) between control and stressed plants. DE transcripts were compared with previous transcriptomes where apomictic and sexual genotypes were contrasted. The results point as candidates to transcripts related to methylation, ubiquitination, hormone and signal transduction pathways, transcription regulation and cell wall biosynthesis, some acting as a general response to stress and some that are specific to the reproductive mode. We suggest that a DNA glycosylase EcROS1-like could be demethylating, thus de-repressing a gene or genes involved in the sexuality pathways. Many of the other DE transcripts could be part of a complex mechanism that regulates apomixis and sexuality in this grass, the ones in the intersection between control/stress and apo/sex being the strongest candidates.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>32825586</pmid><doi>10.3390/genes11090969</doi><orcidid>https://orcid.org/0000-0003-3755-9062</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Abiotic stress Apomixis Argentina Cell walls Cultivars DNA glycosylase DNA methylation Drought Droughts Environmental aspects Epigenetics Eragrostis - genetics Eragrostis - physiology Eragrostis curvula Gene expression Gene Expression Regulation, Plant Gene regulation Genetic aspects Genotypes Grasses MicroRNAs Physiological aspects Pistils Plant asexual reproduction Plant Proteins - genetics Plant Proteins - metabolism Ribonucleic acid RNA Seeds Sequence Analysis, RNA Sexuality Signal transduction Stress (Physiology) Stress, Physiological Transcription Transcriptome Ubiquitination Water stress |
| title | Genes Modulating the Increase in Sexuality in the Facultative Diplosporous Grass Eragrostis curvula under Water Stress Conditions |
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