IRX3/5 regulate mitotic chromatid segregation and limb bud shape
Pattern formation is influenced by transcriptional regulation as well as by morphogenetic mechanisms that shape organ primordia, although factors that link these processes remain under-appreciated. Here we show that, apart from their established transcriptional roles in pattern formation, IRX3/5 hel...
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| Veröffentlicht in: | Development (Cambridge) 2020-10, Vol.147 (19) |
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| creator | Tao, Hirotaka Lambert, Jean-Philippe Yung, Theodora M Zhu, Min Hahn, Noah A Li, Danyi Lau, Kimberly Sturgeon, Kendra Puviindran, Vijitha Zhang, Xiaoyun Gong, Wuming Chen, Xiao Xiao Anderson, Gregory Garry, Daniel J Henkelman, R Mark Sun, Yu Iulianella, Angelo Kawakami, Yasuhiko Gingras, Anne-Claude Hui, Chi-Chung Hopyan, Sevan |
| description | Pattern formation is influenced by transcriptional regulation as well as by morphogenetic mechanisms that shape organ primordia, although factors that link these processes remain under-appreciated. Here we show that, apart from their established transcriptional roles in pattern formation, IRX3/5 help to shape the limb bud primordium by promoting the separation and intercalation of dividing mesodermal cells. Surprisingly, IRX3/5 are required for appropriate cell cycle progression and chromatid segregation during mitosis, possibly in a nontranscriptional manner. IRX3/5 associate with, promote the abundance of, and share overlapping functions with co-regulators of cell division such as the cohesin subunits SMC1, SMC3, NIPBL and CUX1. The findings imply that IRX3/5 coordinate early limb bud morphogenesis with skeletal pattern formation. |
| doi_str_mv | 10.1242/dev.180042 |
| format | Article |
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Here we show that, apart from their established transcriptional roles in pattern formation, IRX3/5 help to shape the limb bud primordium by promoting the separation and intercalation of dividing mesodermal cells. Surprisingly, IRX3/5 are required for appropriate cell cycle progression and chromatid segregation during mitosis, possibly in a nontranscriptional manner. IRX3/5 associate with, promote the abundance of, and share overlapping functions with co-regulators of cell division such as the cohesin subunits SMC1, SMC3, NIPBL and CUX1. The findings imply that IRX3/5 coordinate early limb bud morphogenesis with skeletal pattern formation.</description><identifier>ISSN: 0950-1991</identifier><identifier>EISSN: 1477-9129</identifier><identifier>DOI: 10.1242/dev.180042</identifier><identifier>PMID: 32907847</identifier><language>eng</language><publisher>England: The Company of Biologists Ltd</publisher><subject>Animals ; Blotting, Western ; Chromatids - metabolism ; Chromosome Segregation - genetics ; Chromosome Segregation - physiology ; Female ; Fluorescent Antibody Technique ; HEK293 Cells ; Homeodomain Proteins - genetics ; Homeodomain Proteins - metabolism ; Humans ; Immunoprecipitation ; Limb Buds - embryology ; Limb Buds - metabolism ; Mass Spectrometry ; Mice ; Mitosis - genetics ; Mitosis - physiology ; Pregnancy ; Real-Time Polymerase Chain Reaction ; RNA-Seq ; Transcription Factors - genetics ; Transcription Factors - metabolism</subject><ispartof>Development (Cambridge), 2020-10, Vol.147 (19)</ispartof><rights>2020. 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Published by The Company of Biologists Ltd 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c378t-5f2bfd45a19e8e33cd3cc9914de89ad5f0c3428c903eba00d2dc6d1375305b103</citedby><cites>FETCH-LOGICAL-c378t-5f2bfd45a19e8e33cd3cc9914de89ad5f0c3428c903eba00d2dc6d1375305b103</cites><orcidid>0000-0001-7047-6694 ; 0000-0001-7201-8902 ; 0000-0002-3147-4028 ; 0000-0002-0833-1221 ; 0000-0003-4374-3368</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,777,781,882,3666,27906,27907</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32907847$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tao, Hirotaka</creatorcontrib><creatorcontrib>Lambert, Jean-Philippe</creatorcontrib><creatorcontrib>Yung, Theodora M</creatorcontrib><creatorcontrib>Zhu, Min</creatorcontrib><creatorcontrib>Hahn, Noah A</creatorcontrib><creatorcontrib>Li, Danyi</creatorcontrib><creatorcontrib>Lau, Kimberly</creatorcontrib><creatorcontrib>Sturgeon, Kendra</creatorcontrib><creatorcontrib>Puviindran, Vijitha</creatorcontrib><creatorcontrib>Zhang, Xiaoyun</creatorcontrib><creatorcontrib>Gong, Wuming</creatorcontrib><creatorcontrib>Chen, Xiao Xiao</creatorcontrib><creatorcontrib>Anderson, Gregory</creatorcontrib><creatorcontrib>Garry, Daniel J</creatorcontrib><creatorcontrib>Henkelman, R Mark</creatorcontrib><creatorcontrib>Sun, Yu</creatorcontrib><creatorcontrib>Iulianella, Angelo</creatorcontrib><creatorcontrib>Kawakami, Yasuhiko</creatorcontrib><creatorcontrib>Gingras, Anne-Claude</creatorcontrib><creatorcontrib>Hui, Chi-Chung</creatorcontrib><creatorcontrib>Hopyan, Sevan</creatorcontrib><title>IRX3/5 regulate mitotic chromatid segregation and limb bud shape</title><title>Development (Cambridge)</title><addtitle>Development</addtitle><description>Pattern formation is influenced by transcriptional regulation as well as by morphogenetic mechanisms that shape organ primordia, although factors that link these processes remain under-appreciated. 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The findings imply that IRX3/5 coordinate early limb bud morphogenesis with skeletal pattern formation.</description><subject>Animals</subject><subject>Blotting, Western</subject><subject>Chromatids - metabolism</subject><subject>Chromosome Segregation - genetics</subject><subject>Chromosome Segregation - physiology</subject><subject>Female</subject><subject>Fluorescent Antibody Technique</subject><subject>HEK293 Cells</subject><subject>Homeodomain Proteins - genetics</subject><subject>Homeodomain Proteins - metabolism</subject><subject>Humans</subject><subject>Immunoprecipitation</subject><subject>Limb Buds - embryology</subject><subject>Limb Buds - metabolism</subject><subject>Mass Spectrometry</subject><subject>Mice</subject><subject>Mitosis - genetics</subject><subject>Mitosis - physiology</subject><subject>Pregnancy</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>RNA-Seq</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><issn>0950-1991</issn><issn>1477-9129</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkF1LwzAUhoMobk5v_AHSSxG6nXwtzY0ow4_BQBAF70KapFulbWbSDvz3dmwOvToH3of3HB6ELjGMMWFkYt1mjDMARo7QEDMhUomJPEZDkBxSLCUeoLMYPwGAToU4RQNKJIiMiSG6m79-0AlPglt2lW5dUpetb0uTmFXwtW5Lm0S37NN-9U2iG5tUZZ0nedcHK7125-ik0FV0F_s5Qu-PD2-z53Tx8jSf3S9SQ0XWprwgeWEZ11i6zFFqLDWm_4xZl0lteQGGMpIZCdTlGsASa6YWU8Ep8BwDHaHbXe-6y2tnjWvaoCu1DmWtw7fyulT_k6ZcqaXfKMGnmGWiL7jeFwT_1bnYqrqMxlWVbpzvoiKM4SlISrbozQ41wccYXHE4g0Ftlateudop7-Grv48d0F_H9AfqpHzX</recordid><startdate>20201005</startdate><enddate>20201005</enddate><creator>Tao, Hirotaka</creator><creator>Lambert, Jean-Philippe</creator><creator>Yung, Theodora M</creator><creator>Zhu, Min</creator><creator>Hahn, Noah A</creator><creator>Li, Danyi</creator><creator>Lau, Kimberly</creator><creator>Sturgeon, Kendra</creator><creator>Puviindran, Vijitha</creator><creator>Zhang, Xiaoyun</creator><creator>Gong, Wuming</creator><creator>Chen, Xiao Xiao</creator><creator>Anderson, Gregory</creator><creator>Garry, Daniel J</creator><creator>Henkelman, R Mark</creator><creator>Sun, Yu</creator><creator>Iulianella, Angelo</creator><creator>Kawakami, Yasuhiko</creator><creator>Gingras, Anne-Claude</creator><creator>Hui, Chi-Chung</creator><creator>Hopyan, Sevan</creator><general>The Company of Biologists Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-7047-6694</orcidid><orcidid>https://orcid.org/0000-0001-7201-8902</orcidid><orcidid>https://orcid.org/0000-0002-3147-4028</orcidid><orcidid>https://orcid.org/0000-0002-0833-1221</orcidid><orcidid>https://orcid.org/0000-0003-4374-3368</orcidid></search><sort><creationdate>20201005</creationdate><title>IRX3/5 regulate mitotic chromatid segregation and limb bud shape</title><author>Tao, Hirotaka ; Lambert, Jean-Philippe ; Yung, Theodora M ; Zhu, Min ; Hahn, Noah A ; Li, Danyi ; Lau, Kimberly ; Sturgeon, Kendra ; Puviindran, Vijitha ; Zhang, Xiaoyun ; Gong, Wuming ; Chen, Xiao Xiao ; Anderson, Gregory ; Garry, Daniel J ; Henkelman, R Mark ; Sun, Yu ; Iulianella, Angelo ; Kawakami, Yasuhiko ; Gingras, Anne-Claude ; Hui, Chi-Chung ; Hopyan, Sevan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c378t-5f2bfd45a19e8e33cd3cc9914de89ad5f0c3428c903eba00d2dc6d1375305b103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Blotting, Western</topic><topic>Chromatids - metabolism</topic><topic>Chromosome Segregation - genetics</topic><topic>Chromosome Segregation - physiology</topic><topic>Female</topic><topic>Fluorescent Antibody Technique</topic><topic>HEK293 Cells</topic><topic>Homeodomain Proteins - genetics</topic><topic>Homeodomain Proteins - metabolism</topic><topic>Humans</topic><topic>Immunoprecipitation</topic><topic>Limb Buds - embryology</topic><topic>Limb Buds - metabolism</topic><topic>Mass Spectrometry</topic><topic>Mice</topic><topic>Mitosis - genetics</topic><topic>Mitosis - physiology</topic><topic>Pregnancy</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>RNA-Seq</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tao, Hirotaka</creatorcontrib><creatorcontrib>Lambert, Jean-Philippe</creatorcontrib><creatorcontrib>Yung, Theodora M</creatorcontrib><creatorcontrib>Zhu, Min</creatorcontrib><creatorcontrib>Hahn, Noah A</creatorcontrib><creatorcontrib>Li, Danyi</creatorcontrib><creatorcontrib>Lau, Kimberly</creatorcontrib><creatorcontrib>Sturgeon, Kendra</creatorcontrib><creatorcontrib>Puviindran, Vijitha</creatorcontrib><creatorcontrib>Zhang, Xiaoyun</creatorcontrib><creatorcontrib>Gong, Wuming</creatorcontrib><creatorcontrib>Chen, Xiao Xiao</creatorcontrib><creatorcontrib>Anderson, Gregory</creatorcontrib><creatorcontrib>Garry, Daniel J</creatorcontrib><creatorcontrib>Henkelman, R Mark</creatorcontrib><creatorcontrib>Sun, Yu</creatorcontrib><creatorcontrib>Iulianella, Angelo</creatorcontrib><creatorcontrib>Kawakami, Yasuhiko</creatorcontrib><creatorcontrib>Gingras, Anne-Claude</creatorcontrib><creatorcontrib>Hui, Chi-Chung</creatorcontrib><creatorcontrib>Hopyan, Sevan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Development (Cambridge)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tao, Hirotaka</au><au>Lambert, Jean-Philippe</au><au>Yung, Theodora M</au><au>Zhu, Min</au><au>Hahn, Noah A</au><au>Li, Danyi</au><au>Lau, Kimberly</au><au>Sturgeon, Kendra</au><au>Puviindran, Vijitha</au><au>Zhang, Xiaoyun</au><au>Gong, Wuming</au><au>Chen, Xiao Xiao</au><au>Anderson, Gregory</au><au>Garry, Daniel J</au><au>Henkelman, R Mark</au><au>Sun, Yu</au><au>Iulianella, Angelo</au><au>Kawakami, Yasuhiko</au><au>Gingras, Anne-Claude</au><au>Hui, Chi-Chung</au><au>Hopyan, Sevan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>IRX3/5 regulate mitotic chromatid segregation and limb bud shape</atitle><jtitle>Development (Cambridge)</jtitle><addtitle>Development</addtitle><date>2020-10-05</date><risdate>2020</risdate><volume>147</volume><issue>19</issue><issn>0950-1991</issn><eissn>1477-9129</eissn><abstract>Pattern formation is influenced by transcriptional regulation as well as by morphogenetic mechanisms that shape organ primordia, although factors that link these processes remain under-appreciated. Here we show that, apart from their established transcriptional roles in pattern formation, IRX3/5 help to shape the limb bud primordium by promoting the separation and intercalation of dividing mesodermal cells. Surprisingly, IRX3/5 are required for appropriate cell cycle progression and chromatid segregation during mitosis, possibly in a nontranscriptional manner. IRX3/5 associate with, promote the abundance of, and share overlapping functions with co-regulators of cell division such as the cohesin subunits SMC1, SMC3, NIPBL and CUX1. 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| subjects | Animals Blotting, Western Chromatids - metabolism Chromosome Segregation - genetics Chromosome Segregation - physiology Female Fluorescent Antibody Technique HEK293 Cells Homeodomain Proteins - genetics Homeodomain Proteins - metabolism Humans Immunoprecipitation Limb Buds - embryology Limb Buds - metabolism Mass Spectrometry Mice Mitosis - genetics Mitosis - physiology Pregnancy Real-Time Polymerase Chain Reaction RNA-Seq Transcription Factors - genetics Transcription Factors - metabolism |
| title | IRX3/5 regulate mitotic chromatid segregation and limb bud shape |
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