Selection of endogenous control genes for normalising gene expression data derived from formalin-fixed paraffin-embedded tumour tissue

Quantitative real time polymerase chain reaction (qPCR) data are normalised using endogenous control genes. We aimed to: (1) demonstrate a pathway to identify endogenous control genes for qPCR analysis of formalin-fixed paraffin-embedded (FFPE) tissue using bladder cancer as an exemplar; and (2) exa...

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Veröffentlicht in:	Scientific reports 2020-10, Vol.10 (1), p.17258-17258, Article 17258
Hauptverfasser:	Smith, Tim A. D., AbdelKarem, Omneya A., Irlam-Jones, Joely J., Lane, Brian, Valentine, Helen, Bibby, Becky A. S., Denley, Helen, Choudhury, Ananya, West, Catharine M. L.
Format:	Artikel
Sprache:	eng
Schlagworte:	631/208/199 631/208/69 692/4028/546 Gene Expression Regulation, Neoplastic Humanities and Social Sciences Humans multidisciplinary Neoplasms - metabolism Neoplasms - pathology Paraffin Embedding Prospective Studies Real-Time Polymerase Chain Reaction - standards Reference Standards Regression Analysis Retrospective Studies Ribosomal Proteins - genetics RNA - metabolism RNA - standards Science Science (multidisciplinary) TATA-Box Binding Protein - genetics
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