Construction of a cDNA expression library in a binary vector using a nicking enzyme

Ligation-independent cloning (LIC), such as Gibson Assembly, tends to produce clones without an insert, depending on the sequences present at the ends of linearized vectors. We used a nicking enzyme-mediated LIC (NE-LIC) method to construct a cDNA library in a binary vector pER8. Prior to constructi...

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Veröffentlicht in:	Plant Biotechnology 2020/09/25, Vol.37(3), pp.369-372
Hauptverfasser:	Nishioka, Daiki, Banno, Hiroharu
Format:	Artikel
Sprache:	eng
Schlagworte:	Aquatic life cDNA expression library Cloning Cloning vectors Construction Contamination Enzymes Inserts ligation-independent cloning Nicking endonuclease nicking enzyme Plasmids
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