The double effect of walnut septum extract (Juglans regia L.) counteracts A172 glioblastoma cell survival and bacterial growth

Walnut (Juglans regia L.) is considered to be a 'superfood' for its multiple protective actions on human health. Walnut extracts have proven antitumor activity in different cancer cell lines. However, the efficacy of septum extract against glioblastoma has still not been investigated. Glio...

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Veröffentlicht in:International journal of oncology 2020-11, Vol.57 (5), p.1129-1144
Hauptverfasser: Genovese, Carlo, Cambria, Maria Teresa, D'angeli, Floriana, Addamo, Alessandro Paolo, Malfa, Giuseppe Antonio, Siracusa, Laura, Pulvirenti, Luana, Anfuso, Carmelina Daniela, Lupo, Gabriella, Salmeri, Mario
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container_issue 5
container_start_page 1129
container_title International journal of oncology
container_volume 57
creator Genovese, Carlo
Cambria, Maria Teresa
D'angeli, Floriana
Addamo, Alessandro Paolo
Malfa, Giuseppe Antonio
Siracusa, Laura
Pulvirenti, Luana
Anfuso, Carmelina Daniela
Lupo, Gabriella
Salmeri, Mario
description Walnut (Juglans regia L.) is considered to be a 'superfood' for its multiple protective actions on human health. Walnut extracts have proven antitumor activity in different cancer cell lines. However, the efficacy of septum extract against glioblastoma has still not been investigated. Glioblastoma is the most difficult type of brain cancer to treat. The standard therapy, based on temozolomide, causes several side effects, including neutropenia and lymphocytopenia, which often favor the onset of opportunistic infections. In the present study, the chemical profile of the Sicilian walnut septum ethanolic extract was analyzed using high‑performance liquid chromatography (HPLC)‑diode array detection and HPLC‑electrospray ionization tandem mass spectrometry. The potential cytostatic activity of the extract against the human A172 glioblastoma cell line was investigated and the results showed that the extract could decrease cancer cell proliferation and migration. Using cytofluorimetric analyses and caspase‑3 assays, the pro‑apoptotic action of walnut extract was demonstrated. Furthermore, the evaluation of the antibacterial activity highlighted the efficacy of the extract in reducing Gram‑positive and Gram‑negative bacterial growth, most of which were resistant to the antibiotic, ciprofloxacin. Finally, Prediction of Activity Spectra for Substances analysis showed the predicted antitumor and antibacterial activity of HPLC detected compounds. The promising results could provide novel perspective in the field of chemotherapeutic co‑adjuvants.
doi_str_mv 10.3892/ijo.2020.5130
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Walnut extracts have proven antitumor activity in different cancer cell lines. However, the efficacy of septum extract against glioblastoma has still not been investigated. Glioblastoma is the most difficult type of brain cancer to treat. The standard therapy, based on temozolomide, causes several side effects, including neutropenia and lymphocytopenia, which often favor the onset of opportunistic infections. In the present study, the chemical profile of the Sicilian walnut septum ethanolic extract was analyzed using high‑performance liquid chromatography (HPLC)‑diode array detection and HPLC‑electrospray ionization tandem mass spectrometry. The potential cytostatic activity of the extract against the human A172 glioblastoma cell line was investigated and the results showed that the extract could decrease cancer cell proliferation and migration. Using cytofluorimetric analyses and caspase‑3 assays, the pro‑apoptotic action of walnut extract was demonstrated. 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Furthermore, the evaluation of the antibacterial activity highlighted the efficacy of the extract in reducing Gram‑positive and Gram‑negative bacterial growth, most of which were resistant to the antibiotic, ciprofloxacin. Finally, Prediction of Activity Spectra for Substances analysis showed the predicted antitumor and antibacterial activity of HPLC detected compounds. The promising results could provide novel perspective in the field of chemotherapeutic co‑adjuvants.</abstract><cop>Greece</cop><pub>Spandidos Publications</pub><pmid>33491752</pmid><doi>10.3892/ijo.2020.5130</doi><tpages>16</tpages><oa>free_for_read</oa></addata></record>
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source Spandidos Publications Journals; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection
subjects Acids
Analysis
Antimicrobial agents
Apoptosis
Brain cancer
Brain tumors
Cancer therapies
Chemotherapy
Chromatography
Cytotoxicity
Glioblastomas
Growth
Health aspects
High performance liquid chromatography
Ionization
Medical research
Particle size
Pharmaceutical industry
Scientific equipment industry
Tumors
title The double effect of walnut septum extract (Juglans regia L.) counteracts A172 glioblastoma cell survival and bacterial growth
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