Optimization in Detection of Antigen‐Specific T Cells Through Differentially Labeled MHC Multimers

A large variety of fluorescent molecules are used on a regular basis to tag major histocompatibility complex (MHC) multimers for detection of antigen‐specific T cells. We have evaluated the way in which the choice of fluorescent label can impact the detection of MHC multimer binding T cells in an ex...

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Veröffentlicht in:	Cytometry. Part A 2020-09, Vol.97 (9), p.955-964
Hauptverfasser:	Pedersen, Natasja Wulff, Laske, Karoline, Maurer, Dominik, Welters, Marij, Walter, Steffen, Gouttefangeas, Cécile, Hadrup, Sine Reker
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies antigen specific CD8 T cells Antigens Binding Cytometry flow cytometry Fluorescence Lymphocytes Lymphocytes T Major histocompatibility complex MHC multimers Optimization optimized detection of fluorescence Original Performance evaluation Reagents Staining
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