Assessment of Holographic Microscopy for Quantifying Marine Particle Size and Concentration
Holographic microscopy has emerged as a tool for in situ imaging of microscopic organisms and other particles in the marine environment: appealing because of relatively larger sampling volumes and simpler optical configurations compared to similar imaging systems. However, its quantitative capabilit...
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Veröffentlicht in: | Limnology and oceanography, methods methods, 2020-09, Vol.18 (9), p.516-530 |
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creator | Walcutt, Noah L. Knörlein, Benjamin Cetinic, Ivona Ljubesic, Zrinka Bosak, Suncica Sgouros, Tom Montalbano, Amanda L. Neeley, Aimee Menden-Deuer, Susanne Omand, Melissa M. |
description | Holographic microscopy has emerged as a tool for in situ imaging of microscopic organisms and other particles in the marine environment: appealing because of relatively larger sampling volumes and simpler optical configurations compared to similar imaging systems. However, its quantitative capabilities have remained uncertain, in part because hologram reconstruction and image recognition have required manual operation. Here we assess the quantitative skill of our automated hologram processing pipeline, the CCV Pipeline, to evaluate the size and
concentration measurements of environmental and cultured assemblages of marine plankton particles, and microspheres. Over one-million particles, ranging from 10-200 microns equivalent spherical diameter (ESD), imaged by the 4-Deep HoloSea digital inline holographic microscope (DIHM) are analyzed. These measurements were collected in parallel with FlowCam, Imaging FlowCytobot, and manual microscope identification. Once corrections for particle location and nonuniform illumination were developed and applied, the DIHM showed an underestimate in ESD of about 3-10%, but successfully reproduced the size-spectral-slope from environmental samples, and the size distribution of monocultures and microspheres. DIHM concentrations (order 1-1000 particles~ml-1) showed linear agreement (r^2=0.73) with the other instruments, but individual comparisons at times had large uncertainty. |
doi_str_mv | 10.1002/lom3.10379 |
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concentration measurements of environmental and cultured assemblages of marine plankton particles, and microspheres. Over one-million particles, ranging from 10-200 microns equivalent spherical diameter (ESD), imaged by the 4-Deep HoloSea digital inline holographic microscope (DIHM) are analyzed. These measurements were collected in parallel with FlowCam, Imaging FlowCytobot, and manual microscope identification. Once corrections for particle location and nonuniform illumination were developed and applied, the DIHM showed an underestimate in ESD of about 3-10%, but successfully reproduced the size-spectral-slope from environmental samples, and the size distribution of monocultures and microspheres. DIHM concentrations (order 1-1000 particles~ml-1) showed linear agreement (r^2=0.73) with the other instruments, but individual comparisons at times had large uncertainty.</description><identifier>ISSN: 1541-5856</identifier><identifier>EISSN: 1541-5856</identifier><identifier>DOI: 10.1002/lom3.10379</identifier><identifier>PMID: 33041697</identifier><language>eng</language><publisher>Goddard Space Flight Center: Association for the Sciences of Limnology and Oceanography</publisher><subject>Comparative and Intercalibration Studies ; Geosciences (General) ; Instrumentation And Photography</subject><ispartof>Limnology and oceanography, methods, 2020-09, Vol.18 (9), p.516-530</ispartof><rights>Copyright Determination: PUBLIC_USE_PERMITTED</rights><rights>2020 The Authors. published by Wiley Periodicals LLC. on behalf of Association for the Sciences of Limnology and Oceanography.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4189-2abdeeb4c031a4443e8975b2217c09503ce085818de857529ca00fe1b9bdc4d83</citedby><cites>FETCH-LOGICAL-c4189-2abdeeb4c031a4443e8975b2217c09503ce085818de857529ca00fe1b9bdc4d83</cites><orcidid>0000-0002-8434-4251 ; 0000-0001-5701-0953 ; 0000-0001-7301-9894 ; 0000-0002-1363-3136</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Flom3.10379$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Flom3.10379$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,796,881,1411,1427,27903,27904,45553,45554,46387,46811</link.rule.ids></links><search><creatorcontrib>Walcutt, Noah L.</creatorcontrib><creatorcontrib>Knörlein, Benjamin</creatorcontrib><creatorcontrib>Cetinic, Ivona</creatorcontrib><creatorcontrib>Ljubesic, Zrinka</creatorcontrib><creatorcontrib>Bosak, Suncica</creatorcontrib><creatorcontrib>Sgouros, Tom</creatorcontrib><creatorcontrib>Montalbano, Amanda L.</creatorcontrib><creatorcontrib>Neeley, Aimee</creatorcontrib><creatorcontrib>Menden-Deuer, Susanne</creatorcontrib><creatorcontrib>Omand, Melissa M.</creatorcontrib><title>Assessment of Holographic Microscopy for Quantifying Marine Particle Size and Concentration</title><title>Limnology and oceanography, methods</title><description>Holographic microscopy has emerged as a tool for in situ imaging of microscopic organisms and other particles in the marine environment: appealing because of relatively larger sampling volumes and simpler optical configurations compared to similar imaging systems. However, its quantitative capabilities have remained uncertain, in part because hologram reconstruction and image recognition have required manual operation. Here we assess the quantitative skill of our automated hologram processing pipeline, the CCV Pipeline, to evaluate the size and
concentration measurements of environmental and cultured assemblages of marine plankton particles, and microspheres. Over one-million particles, ranging from 10-200 microns equivalent spherical diameter (ESD), imaged by the 4-Deep HoloSea digital inline holographic microscope (DIHM) are analyzed. These measurements were collected in parallel with FlowCam, Imaging FlowCytobot, and manual microscope identification. Once corrections for particle location and nonuniform illumination were developed and applied, the DIHM showed an underestimate in ESD of about 3-10%, but successfully reproduced the size-spectral-slope from environmental samples, and the size distribution of monocultures and microspheres. 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concentration measurements of environmental and cultured assemblages of marine plankton particles, and microspheres. Over one-million particles, ranging from 10-200 microns equivalent spherical diameter (ESD), imaged by the 4-Deep HoloSea digital inline holographic microscope (DIHM) are analyzed. These measurements were collected in parallel with FlowCam, Imaging FlowCytobot, and manual microscope identification. Once corrections for particle location and nonuniform illumination were developed and applied, the DIHM showed an underestimate in ESD of about 3-10%, but successfully reproduced the size-spectral-slope from environmental samples, and the size distribution of monocultures and microspheres. DIHM concentrations (order 1-1000 particles~ml-1) showed linear agreement (r^2=0.73) with the other instruments, but individual comparisons at times had large uncertainty.</abstract><cop>Goddard Space Flight Center</cop><pub>Association for the Sciences of Limnology and Oceanography</pub><pmid>33041697</pmid><doi>10.1002/lom3.10379</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0002-8434-4251</orcidid><orcidid>https://orcid.org/0000-0001-5701-0953</orcidid><orcidid>https://orcid.org/0000-0001-7301-9894</orcidid><orcidid>https://orcid.org/0000-0002-1363-3136</orcidid><oa>free_for_read</oa></addata></record> |
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title | Assessment of Holographic Microscopy for Quantifying Marine Particle Size and Concentration |
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