Surveillance of Shiga toxin-producing Escherichia coli and Campylobacter spp. in wild Japanese deer (Cervus nippon) and boar (Sus scrofa)
Increasing game meat consumption in Japan requires the dissemination of safety information regarding the presence of human pathogens in game animals. Health information regarding the suitability of these animals as a meat source is not widely available. In this study, we aimed to evaluate the safety...
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| Veröffentlicht in: | Journal of Veterinary Medical Science 2020, Vol.82(9), pp.1287-1294 |
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| creator | TOMINO, Yoshiyuki ANDOH, Masako HORIUCHI, Yuta SHIN, Jiye AI, Ryunosuke NAKAMURA, Takaki TODA, Mizuki YONEMITSU, Kenzo TAKANO, Ai SHIMODA, Hiroshi MAEDA, Ken KODERA, Yuuji OSHIMA, Ichiro TAKAYAMA, Koji INADOME, Takayasu SHIOYA, Katsunori FUKAZAWA, Motoki ISHIHARA, Kanako CHUMA, Takehisa |
| description | Increasing game meat consumption in Japan requires the dissemination of safety information regarding the presence of human pathogens in game animals. Health information regarding the suitability of these animals as a meat source is not widely available. In this study, we aimed to evaluate the safety of game meat and detect potential human pathogens in wild deer (Cervus nippon) and boar (Sus scrofa) in Japan. Fecal samples from 305 wild deer and 248 boars of Yamaguchi, Kagoshima, and Tochigi prefectures collected monthly for 2 years were examined for the prevalence of Shiga toxin-producing Escherichia coli (STEC) and Campylobacter spp. STEC was isolated from 51 deer consistently throughout the year and from three boars; O-antigen genotype O146, the expression of stx2b, and eaeA absence (n=33) were the major characteristics of our STEC isolates. Other serotypes included the medically important O157, stx2b or stx2c, and eaeA-positive (n=4) and O26, stx1a, and eaeA-positive strains (n=1). Campylobacter spp. were isolated from 17 deer and 31 boars. Campylobacter hyointestinalis was the most common species isolated from 17 deer and 25 boars, whereas Campylobacter lanienae and Campylobacter coli were isolated from three and two boars, respectively. Seasonal trends for the isolation of these bacteria were not significant. This study demonstrates that wild game animals carry human pathogens; therefore, detailed knowledge of the safe handling of game meat is needed to prevent foodborne infections. |
| doi_str_mv | 10.1292/jvms.19-0265 |
| format | Article |
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Health information regarding the suitability of these animals as a meat source is not widely available. In this study, we aimed to evaluate the safety of game meat and detect potential human pathogens in wild deer (Cervus nippon) and boar (Sus scrofa) in Japan. Fecal samples from 305 wild deer and 248 boars of Yamaguchi, Kagoshima, and Tochigi prefectures collected monthly for 2 years were examined for the prevalence of Shiga toxin-producing Escherichia coli (STEC) and Campylobacter spp. STEC was isolated from 51 deer consistently throughout the year and from three boars; O-antigen genotype O146, the expression of stx2b, and eaeA absence (n=33) were the major characteristics of our STEC isolates. Other serotypes included the medically important O157, stx2b or stx2c, and eaeA-positive (n=4) and O26, stx1a, and eaeA-positive strains (n=1). Campylobacter spp. were isolated from 17 deer and 31 boars. Campylobacter hyointestinalis was the most common species isolated from 17 deer and 25 boars, whereas Campylobacter lanienae and Campylobacter coli were isolated from three and two boars, respectively. Seasonal trends for the isolation of these bacteria were not significant. This study demonstrates that wild game animals carry human pathogens; therefore, detailed knowledge of the safe handling of game meat is needed to prevent foodborne infections.</description><identifier>ISSN: 0916-7250</identifier><identifier>EISSN: 1347-7439</identifier><identifier>DOI: 10.1292/jvms.19-0265</identifier><identifier>PMID: 32655094</identifier><language>eng</language><publisher>Tokyo: JAPANESE SOCIETY OF VETERINARY SCIENCE</publisher><subject>boar ; Campylobacter ; Cervus nippon ; Deer ; E coli ; Escherichia coli ; Game animals ; game meat safety ; Genotypes ; Meat ; Pathogens ; Public Health ; Serotypes ; Shiga toxin ; Shiga toxin-producing Escherichia coli ; Sus scrofa</subject><ispartof>Journal of Veterinary Medical Science, 2020, Vol.82(9), pp.1287-1294</ispartof><rights>2020 by the Japanese Society of Veterinary Science</rights><rights>2020. This work is published under https://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 The Japanese Society of Veterinary Science 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c639t-c0b2f33b20287cb1e547341e521893531990287b83ecb3c34fe74c50c5a6211a3</citedby><cites>FETCH-LOGICAL-c639t-c0b2f33b20287cb1e547341e521893531990287b83ecb3c34fe74c50c5a6211a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7538328/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7538328/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,1877,4010,27900,27901,27902,53766,53768</link.rule.ids></links><search><creatorcontrib>TOMINO, Yoshiyuki</creatorcontrib><creatorcontrib>ANDOH, Masako</creatorcontrib><creatorcontrib>HORIUCHI, Yuta</creatorcontrib><creatorcontrib>SHIN, Jiye</creatorcontrib><creatorcontrib>AI, Ryunosuke</creatorcontrib><creatorcontrib>NAKAMURA, Takaki</creatorcontrib><creatorcontrib>TODA, Mizuki</creatorcontrib><creatorcontrib>YONEMITSU, Kenzo</creatorcontrib><creatorcontrib>TAKANO, Ai</creatorcontrib><creatorcontrib>SHIMODA, Hiroshi</creatorcontrib><creatorcontrib>MAEDA, Ken</creatorcontrib><creatorcontrib>KODERA, Yuuji</creatorcontrib><creatorcontrib>OSHIMA, Ichiro</creatorcontrib><creatorcontrib>TAKAYAMA, Koji</creatorcontrib><creatorcontrib>INADOME, Takayasu</creatorcontrib><creatorcontrib>SHIOYA, Katsunori</creatorcontrib><creatorcontrib>FUKAZAWA, Motoki</creatorcontrib><creatorcontrib>ISHIHARA, Kanako</creatorcontrib><creatorcontrib>CHUMA, Takehisa</creatorcontrib><title>Surveillance of Shiga toxin-producing Escherichia coli and Campylobacter spp. in wild Japanese deer (Cervus nippon) and boar (Sus scrofa)</title><title>Journal of Veterinary Medical Science</title><addtitle>J. Vet. Med. Sci.</addtitle><description>Increasing game meat consumption in Japan requires the dissemination of safety information regarding the presence of human pathogens in game animals. Health information regarding the suitability of these animals as a meat source is not widely available. In this study, we aimed to evaluate the safety of game meat and detect potential human pathogens in wild deer (Cervus nippon) and boar (Sus scrofa) in Japan. Fecal samples from 305 wild deer and 248 boars of Yamaguchi, Kagoshima, and Tochigi prefectures collected monthly for 2 years were examined for the prevalence of Shiga toxin-producing Escherichia coli (STEC) and Campylobacter spp. STEC was isolated from 51 deer consistently throughout the year and from three boars; O-antigen genotype O146, the expression of stx2b, and eaeA absence (n=33) were the major characteristics of our STEC isolates. Other serotypes included the medically important O157, stx2b or stx2c, and eaeA-positive (n=4) and O26, stx1a, and eaeA-positive strains (n=1). Campylobacter spp. were isolated from 17 deer and 31 boars. Campylobacter hyointestinalis was the most common species isolated from 17 deer and 25 boars, whereas Campylobacter lanienae and Campylobacter coli were isolated from three and two boars, respectively. Seasonal trends for the isolation of these bacteria were not significant. This study demonstrates that wild game animals carry human pathogens; therefore, detailed knowledge of the safe handling of game meat is needed to prevent foodborne infections.</description><subject>boar</subject><subject>Campylobacter</subject><subject>Cervus nippon</subject><subject>Deer</subject><subject>E coli</subject><subject>Escherichia coli</subject><subject>Game animals</subject><subject>game meat safety</subject><subject>Genotypes</subject><subject>Meat</subject><subject>Pathogens</subject><subject>Public Health</subject><subject>Serotypes</subject><subject>Shiga toxin</subject><subject>Shiga toxin-producing Escherichia coli</subject><subject>Sus scrofa</subject><issn>0916-7250</issn><issn>1347-7439</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNpVkd2O0zAQhS0EYsvCHQ9giRtW2hT_JY5vkKBa_rQSF4Vra-I4javUDnZS2EfYt8ahVSVuZqQ535yxdRB6TcmaMsXe7Y-HtKaqIKwqn6AV5UIWUnD1FK2IolUhWUmu0IuU9oQwKir1HF3xzJZEiRV63M7xaN0wgDcWhw5ve7cDPIU_zhdjDO1snN_hu2R6G53pHWATBofBt3gDh_FhCA2YyUacxnGNnce_3dDibzCCt8ni1mbp7cbG45ywd-MY_M2_5SZAFrZ5mkwMHdy8RM86GJJ9de7X6Oenux-bL8X9989fNx_uC1NxNRWGNKzjvGGE1dI01JZCcpEbo7XiJadKLUpTc2sabrjorBSmJKaEilEK_Bq9P_mOc3OwrbF-ijDoMboDxAcdwOn_Fe96vQtHLUtec1Zngzdngxh-zTZNeh_m6PObNRNCKkl5RTN1e6Ly71KKtrtcoEQvweklOE2VXoLL-McTvk8T7OwFhjg5M9gTXDOtlnJeuoimh6it538B_oejvw</recordid><startdate>2020</startdate><enddate>2020</enddate><creator>TOMINO, Yoshiyuki</creator><creator>ANDOH, Masako</creator><creator>HORIUCHI, Yuta</creator><creator>SHIN, Jiye</creator><creator>AI, Ryunosuke</creator><creator>NAKAMURA, Takaki</creator><creator>TODA, Mizuki</creator><creator>YONEMITSU, Kenzo</creator><creator>TAKANO, Ai</creator><creator>SHIMODA, Hiroshi</creator><creator>MAEDA, Ken</creator><creator>KODERA, Yuuji</creator><creator>OSHIMA, Ichiro</creator><creator>TAKAYAMA, Koji</creator><creator>INADOME, Takayasu</creator><creator>SHIOYA, Katsunori</creator><creator>FUKAZAWA, Motoki</creator><creator>ISHIHARA, Kanako</creator><creator>CHUMA, Takehisa</creator><general>JAPANESE SOCIETY OF VETERINARY SCIENCE</general><general>Japan Science and Technology Agency</general><general>The Japanese Society of Veterinary Science</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>5PM</scope></search><sort><creationdate>2020</creationdate><title>Surveillance of Shiga toxin-producing Escherichia coli and Campylobacter spp. in wild Japanese deer (Cervus nippon) and boar (Sus scrofa)</title><author>TOMINO, Yoshiyuki ; ANDOH, Masako ; HORIUCHI, Yuta ; SHIN, Jiye ; AI, Ryunosuke ; NAKAMURA, Takaki ; TODA, Mizuki ; YONEMITSU, Kenzo ; TAKANO, Ai ; SHIMODA, Hiroshi ; MAEDA, Ken ; KODERA, Yuuji ; OSHIMA, Ichiro ; TAKAYAMA, Koji ; INADOME, Takayasu ; SHIOYA, Katsunori ; FUKAZAWA, Motoki ; ISHIHARA, Kanako ; CHUMA, Takehisa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c639t-c0b2f33b20287cb1e547341e521893531990287b83ecb3c34fe74c50c5a6211a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>boar</topic><topic>Campylobacter</topic><topic>Cervus nippon</topic><topic>Deer</topic><topic>E coli</topic><topic>Escherichia coli</topic><topic>Game animals</topic><topic>game meat safety</topic><topic>Genotypes</topic><topic>Meat</topic><topic>Pathogens</topic><topic>Public Health</topic><topic>Serotypes</topic><topic>Shiga toxin</topic><topic>Shiga toxin-producing Escherichia coli</topic><topic>Sus scrofa</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>TOMINO, Yoshiyuki</creatorcontrib><creatorcontrib>ANDOH, Masako</creatorcontrib><creatorcontrib>HORIUCHI, Yuta</creatorcontrib><creatorcontrib>SHIN, Jiye</creatorcontrib><creatorcontrib>AI, Ryunosuke</creatorcontrib><creatorcontrib>NAKAMURA, Takaki</creatorcontrib><creatorcontrib>TODA, Mizuki</creatorcontrib><creatorcontrib>YONEMITSU, Kenzo</creatorcontrib><creatorcontrib>TAKANO, Ai</creatorcontrib><creatorcontrib>SHIMODA, Hiroshi</creatorcontrib><creatorcontrib>MAEDA, Ken</creatorcontrib><creatorcontrib>KODERA, Yuuji</creatorcontrib><creatorcontrib>OSHIMA, Ichiro</creatorcontrib><creatorcontrib>TAKAYAMA, Koji</creatorcontrib><creatorcontrib>INADOME, Takayasu</creatorcontrib><creatorcontrib>SHIOYA, Katsunori</creatorcontrib><creatorcontrib>FUKAZAWA, Motoki</creatorcontrib><creatorcontrib>ISHIHARA, Kanako</creatorcontrib><creatorcontrib>CHUMA, Takehisa</creatorcontrib><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Veterinary Medical Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>TOMINO, Yoshiyuki</au><au>ANDOH, Masako</au><au>HORIUCHI, Yuta</au><au>SHIN, Jiye</au><au>AI, Ryunosuke</au><au>NAKAMURA, Takaki</au><au>TODA, Mizuki</au><au>YONEMITSU, Kenzo</au><au>TAKANO, Ai</au><au>SHIMODA, Hiroshi</au><au>MAEDA, Ken</au><au>KODERA, Yuuji</au><au>OSHIMA, Ichiro</au><au>TAKAYAMA, Koji</au><au>INADOME, Takayasu</au><au>SHIOYA, Katsunori</au><au>FUKAZAWA, Motoki</au><au>ISHIHARA, Kanako</au><au>CHUMA, Takehisa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Surveillance of Shiga toxin-producing Escherichia coli and Campylobacter spp. in wild Japanese deer (Cervus nippon) and boar (Sus scrofa)</atitle><jtitle>Journal of Veterinary Medical Science</jtitle><addtitle>J. Vet. Med. Sci.</addtitle><date>2020</date><risdate>2020</risdate><volume>82</volume><issue>9</issue><spage>1287</spage><epage>1294</epage><pages>1287-1294</pages><issn>0916-7250</issn><eissn>1347-7439</eissn><abstract>Increasing game meat consumption in Japan requires the dissemination of safety information regarding the presence of human pathogens in game animals. Health information regarding the suitability of these animals as a meat source is not widely available. In this study, we aimed to evaluate the safety of game meat and detect potential human pathogens in wild deer (Cervus nippon) and boar (Sus scrofa) in Japan. Fecal samples from 305 wild deer and 248 boars of Yamaguchi, Kagoshima, and Tochigi prefectures collected monthly for 2 years were examined for the prevalence of Shiga toxin-producing Escherichia coli (STEC) and Campylobacter spp. STEC was isolated from 51 deer consistently throughout the year and from three boars; O-antigen genotype O146, the expression of stx2b, and eaeA absence (n=33) were the major characteristics of our STEC isolates. Other serotypes included the medically important O157, stx2b or stx2c, and eaeA-positive (n=4) and O26, stx1a, and eaeA-positive strains (n=1). Campylobacter spp. were isolated from 17 deer and 31 boars. Campylobacter hyointestinalis was the most common species isolated from 17 deer and 25 boars, whereas Campylobacter lanienae and Campylobacter coli were isolated from three and two boars, respectively. Seasonal trends for the isolation of these bacteria were not significant. This study demonstrates that wild game animals carry human pathogens; therefore, detailed knowledge of the safe handling of game meat is needed to prevent foodborne infections.</abstract><cop>Tokyo</cop><pub>JAPANESE SOCIETY OF VETERINARY SCIENCE</pub><pmid>32655094</pmid><doi>10.1292/jvms.19-0265</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of Veterinary Medical Science, 2020, Vol.82(9), pp.1287-1294 |
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| source | PubMed (Medline); J-STAGE (Free - Japanese); EZB Electronic Journals Library; PubMed Central Open Access |
| subjects | boar Campylobacter Cervus nippon Deer E coli Escherichia coli Game animals game meat safety Genotypes Meat Pathogens Public Health Serotypes Shiga toxin Shiga toxin-producing Escherichia coli Sus scrofa |
| title | Surveillance of Shiga toxin-producing Escherichia coli and Campylobacter spp. in wild Japanese deer (Cervus nippon) and boar (Sus scrofa) |
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