MMP-7 derived peptides with MHC class-I binding motifs from canine mammary tumor tissue elicit strong antigen-specific T-cell responses in BALB/c mice
Matrix Metalloproteinases (MMPs)-induced altered proteolysis of extracellular matrix proteins and basement membrane holds the key for tumor progression and metastasis. Matrix metalloproteinases-7 (Matrilysin), the smallest member of the MMP family also performs quite alike; thus serves as a potentia...
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| Veröffentlicht in: | Molecular and cellular biochemistry 2021-01, Vol.476 (1), p.311-320 |
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| creator | Yadav, Pavan Kumar Gupta, Shishir Kumar Kumar, Saroj Ghosh, Mayukh Yadav, Brijesh Singh Kumar, Dinesh Kumar, Ajay Saini, Mohini Kataria, Meena |
| description | Matrix Metalloproteinases (MMPs)-induced altered proteolysis of extracellular matrix proteins and basement membrane holds the key for tumor progression and metastasis. Matrix metalloproteinases-7 (Matrilysin), the smallest member of the MMP family also performs quite alike; thus serves as a potential candidate for anti-tumor immunotherapy. Conversely, being an endogenous tumor-associated antigen (TAA), targeting MMP-7 for immunization is challenging. But MMP-7-based xenovaccine can surmount the obstacle of poor immunogenicity and immunological tolerance, often encountered in TAA-based conventional vaccine for anti-tumor immunotherapy. This paves the way for investigating the potential of MMP-7-derived major histocompatibility complex (MHC)-binding peptides to elicit precise epitope-specific T-cell responses towards their possible inclusion in anti-tumor vaccine formulations. Perhaps it also ushers the path of achieving multiple epitope-based broad and universal cellular immunity. In current experiment, an immunoinformatics approach has been employed to identify the putative canine matrix matelloproteinases-7 (cMMP-7)-derived peptides with MHC class-I-binding motifs which can elicit potent antigen-specific immune responses in BALB/c mice. Immunization with the cMMP-7 DNA vaccine induced a strong CD8
+
cytotoxic T lymphocytes (CTLs) and Th1- type response, with high level of gamma interferon (IFN-γ) production in BALB/c mice. The two identified putative MHC-I-binding nonameric peptides (Peptide
32-40
and Peptide
175-183
) from cMMP-7 induced significant lymphocyte proliferation along with the production of IFN-γ from CD8
+
T-cells in mice immunized with cMMP-7 DNA vaccine. The current observation has depicted the immunogenic potential of the two cMMP-7-derived nonapeptides for their possible exploitation in xenovaccine-mediated anti-tumor immunotherapy in mouse model. |
| doi_str_mv | 10.1007/s11010-020-03908-2 |
| format | Article |
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+
cytotoxic T lymphocytes (CTLs) and Th1- type response, with high level of gamma interferon (IFN-γ) production in BALB/c mice. The two identified putative MHC-I-binding nonameric peptides (Peptide
32-40
and Peptide
175-183
) from cMMP-7 induced significant lymphocyte proliferation along with the production of IFN-γ from CD8
+
T-cells in mice immunized with cMMP-7 DNA vaccine. The current observation has depicted the immunogenic potential of the two cMMP-7-derived nonapeptides for their possible exploitation in xenovaccine-mediated anti-tumor immunotherapy in mouse model.</description><identifier>ISSN: 0300-8177</identifier><identifier>EISSN: 1573-4919</identifier><identifier>DOI: 10.1007/s11010-020-03908-2</identifier><identifier>PMID: 32970284</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Animals ; Antigen (tumor-associated) ; Antigenic determinants ; Antigens ; Antigens, Neoplasm - metabolism ; Binding ; Biochemistry ; Biomedical and Life Sciences ; Breast cancer ; Cancer Vaccines ; Cardiology ; CD8 antigen ; Cell Line, Tumor ; Cell Proliferation ; Cell-mediated immunity ; Computational Biology ; Cytotoxicity ; Deoxyribonucleic acid ; DNA ; DNA vaccines ; Dogs ; Epitopes ; Epitopes - chemistry ; Extracellular matrix ; Female ; HEK293 Cells ; Histocompatibility Antigens Class I - immunology ; Humans ; Immune response ; Immunization ; Immunogenicity ; Immunoglobulin G - immunology ; Immunological tolerance ; Immunology ; Immunotherapy ; Immunotherapy - methods ; Interferon ; Interferon-gamma ; Life Sciences ; Lymphocyte Activation ; Lymphocytes ; Lymphocytes T ; Major histocompatibility complex ; Mammary gland ; Mammary Glands, Animal - metabolism ; Matrilysin ; Matrix metalloproteinase ; Matrix Metalloproteinase 7 - metabolism ; Matrix metalloproteinases ; Medical Biochemistry ; Metastases ; Mice ; Mice, Inbred BALB C ; Oncology ; Peptides ; Peptides - chemistry ; Protein Binding ; Proteolysis ; T cells ; T-Lymphocytes - cytology ; T-Lymphocytes - immunology ; T-Lymphocytes, Cytotoxic - immunology ; Tumors ; Vaccines ; γ-Interferon</subject><ispartof>Molecular and cellular biochemistry, 2021-01, Vol.476 (1), p.311-320</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020</rights><rights>COPYRIGHT 2020 Springer</rights><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c541t-52f91ceaceb8278e064e69ab9f385e1e091c00dec9a0d8c4bc6e572c4e79a7123</citedby><cites>FETCH-LOGICAL-c541t-52f91ceaceb8278e064e69ab9f385e1e091c00dec9a0d8c4bc6e572c4e79a7123</cites><orcidid>0000-0002-1847-7486</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11010-020-03908-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11010-020-03908-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,780,784,885,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32970284$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yadav, Pavan Kumar</creatorcontrib><creatorcontrib>Gupta, Shishir Kumar</creatorcontrib><creatorcontrib>Kumar, Saroj</creatorcontrib><creatorcontrib>Ghosh, Mayukh</creatorcontrib><creatorcontrib>Yadav, Brijesh Singh</creatorcontrib><creatorcontrib>Kumar, Dinesh</creatorcontrib><creatorcontrib>Kumar, Ajay</creatorcontrib><creatorcontrib>Saini, Mohini</creatorcontrib><creatorcontrib>Kataria, Meena</creatorcontrib><title>MMP-7 derived peptides with MHC class-I binding motifs from canine mammary tumor tissue elicit strong antigen-specific T-cell responses in BALB/c mice</title><title>Molecular and cellular biochemistry</title><addtitle>Mol Cell Biochem</addtitle><addtitle>Mol Cell Biochem</addtitle><description>Matrix Metalloproteinases (MMPs)-induced altered proteolysis of extracellular matrix proteins and basement membrane holds the key for tumor progression and metastasis. Matrix metalloproteinases-7 (Matrilysin), the smallest member of the MMP family also performs quite alike; thus serves as a potential candidate for anti-tumor immunotherapy. Conversely, being an endogenous tumor-associated antigen (TAA), targeting MMP-7 for immunization is challenging. But MMP-7-based xenovaccine can surmount the obstacle of poor immunogenicity and immunological tolerance, often encountered in TAA-based conventional vaccine for anti-tumor immunotherapy. This paves the way for investigating the potential of MMP-7-derived major histocompatibility complex (MHC)-binding peptides to elicit precise epitope-specific T-cell responses towards their possible inclusion in anti-tumor vaccine formulations. Perhaps it also ushers the path of achieving multiple epitope-based broad and universal cellular immunity. In current experiment, an immunoinformatics approach has been employed to identify the putative canine matrix matelloproteinases-7 (cMMP-7)-derived peptides with MHC class-I-binding motifs which can elicit potent antigen-specific immune responses in BALB/c mice. Immunization with the cMMP-7 DNA vaccine induced a strong CD8
+
cytotoxic T lymphocytes (CTLs) and Th1- type response, with high level of gamma interferon (IFN-γ) production in BALB/c mice. The two identified putative MHC-I-binding nonameric peptides (Peptide
32-40
and Peptide
175-183
) from cMMP-7 induced significant lymphocyte proliferation along with the production of IFN-γ from CD8
+
T-cells in mice immunized with cMMP-7 DNA vaccine. The current observation has depicted the immunogenic potential of the two cMMP-7-derived nonapeptides for their possible exploitation in xenovaccine-mediated anti-tumor immunotherapy in mouse model.</description><subject>Animals</subject><subject>Antigen (tumor-associated)</subject><subject>Antigenic determinants</subject><subject>Antigens</subject><subject>Antigens, Neoplasm - metabolism</subject><subject>Binding</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Breast cancer</subject><subject>Cancer Vaccines</subject><subject>Cardiology</subject><subject>CD8 antigen</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation</subject><subject>Cell-mediated immunity</subject><subject>Computational Biology</subject><subject>Cytotoxicity</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA vaccines</subject><subject>Dogs</subject><subject>Epitopes</subject><subject>Epitopes - chemistry</subject><subject>Extracellular matrix</subject><subject>Female</subject><subject>HEK293 Cells</subject><subject>Histocompatibility Antigens Class I - immunology</subject><subject>Humans</subject><subject>Immune response</subject><subject>Immunization</subject><subject>Immunogenicity</subject><subject>Immunoglobulin G - immunology</subject><subject>Immunological tolerance</subject><subject>Immunology</subject><subject>Immunotherapy</subject><subject>Immunotherapy - methods</subject><subject>Interferon</subject><subject>Interferon-gamma</subject><subject>Life Sciences</subject><subject>Lymphocyte Activation</subject><subject>Lymphocytes</subject><subject>Lymphocytes T</subject><subject>Major histocompatibility complex</subject><subject>Mammary gland</subject><subject>Mammary Glands, Animal - metabolism</subject><subject>Matrilysin</subject><subject>Matrix metalloproteinase</subject><subject>Matrix Metalloproteinase 7 - metabolism</subject><subject>Matrix metalloproteinases</subject><subject>Medical Biochemistry</subject><subject>Metastases</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Oncology</subject><subject>Peptides</subject><subject>Peptides - chemistry</subject><subject>Protein Binding</subject><subject>Proteolysis</subject><subject>T cells</subject><subject>T-Lymphocytes - cytology</subject><subject>T-Lymphocytes - immunology</subject><subject>T-Lymphocytes, Cytotoxic - immunology</subject><subject>Tumors</subject><subject>Vaccines</subject><subject>γ-Interferon</subject><issn>0300-8177</issn><issn>1573-4919</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9ksFu1DAQhiMEokvhBTggS1y4pLWdOLYvSNsV0Eq7gkM5W44z2bpK7GA7rXgRnheHLS1FCFmWJc83_-gf_UXxmuATgjE_jYRggktM860kFiV9UqwI41VZSyKfFitcYVwKwvlR8SLGa5xpTMjz4qiikmMq6lXxY7f7UnLUQbA30KEJpmQ7iOjWpiu0O98gM-gYywvUWtdZt0ejT7aPqA9-REY76wCNehx1-I7SPPqAko1xBgSDNTahmILPXdoluwdXxgmM7a1Bl6WBYUAB4uRdzAOtQ2fr7dmpQaM18LJ41ushwqu797j4-vHD5ea83H7-dLFZb0vDapJKRntJDGgDraBcAG5qaKRuZV8JBgRwrmLcgZEad8LUrWmAcWpq4FJzQqvj4v1Bd5rbEToDLgU9qCnYxZHy2qrHFWev1N7fKM4IYXQReHcnEPy3GWJSo42LNe3Az1HRumaykZI1GX37F3rt5-CyvUyJRghWY_5A7fUAyrre57lmEVXrhhHMRN2wTJ38g8qng7w-76C3-f9RAz00mOBjDNDfeyRYLWlShzSpnCb1K01qMffmz-3ct_yOTwaqAxBzye0hPFj6j-xP6B_Veg</recordid><startdate>20210101</startdate><enddate>20210101</enddate><creator>Yadav, Pavan Kumar</creator><creator>Gupta, Shishir Kumar</creator><creator>Kumar, Saroj</creator><creator>Ghosh, Mayukh</creator><creator>Yadav, Brijesh Singh</creator><creator>Kumar, Dinesh</creator><creator>Kumar, Ajay</creator><creator>Saini, Mohini</creator><creator>Kataria, Meena</creator><general>Springer US</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-1847-7486</orcidid></search><sort><creationdate>20210101</creationdate><title>MMP-7 derived peptides with MHC class-I binding motifs from canine mammary tumor tissue elicit strong antigen-specific T-cell responses in BALB/c mice</title><author>Yadav, Pavan Kumar ; Gupta, Shishir Kumar ; Kumar, Saroj ; Ghosh, Mayukh ; Yadav, Brijesh Singh ; Kumar, Dinesh ; Kumar, Ajay ; Saini, Mohini ; Kataria, Meena</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c541t-52f91ceaceb8278e064e69ab9f385e1e091c00dec9a0d8c4bc6e572c4e79a7123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>Antigen (tumor-associated)</topic><topic>Antigenic determinants</topic><topic>Antigens</topic><topic>Antigens, Neoplasm - metabolism</topic><topic>Binding</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Breast cancer</topic><topic>Cancer Vaccines</topic><topic>Cardiology</topic><topic>CD8 antigen</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation</topic><topic>Cell-mediated immunity</topic><topic>Computational Biology</topic><topic>Cytotoxicity</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA vaccines</topic><topic>Dogs</topic><topic>Epitopes</topic><topic>Epitopes - chemistry</topic><topic>Extracellular matrix</topic><topic>Female</topic><topic>HEK293 Cells</topic><topic>Histocompatibility Antigens Class I - immunology</topic><topic>Humans</topic><topic>Immune response</topic><topic>Immunization</topic><topic>Immunogenicity</topic><topic>Immunoglobulin G - immunology</topic><topic>Immunological tolerance</topic><topic>Immunology</topic><topic>Immunotherapy</topic><topic>Immunotherapy - methods</topic><topic>Interferon</topic><topic>Interferon-gamma</topic><topic>Life Sciences</topic><topic>Lymphocyte Activation</topic><topic>Lymphocytes</topic><topic>Lymphocytes T</topic><topic>Major histocompatibility complex</topic><topic>Mammary gland</topic><topic>Mammary Glands, Animal - metabolism</topic><topic>Matrilysin</topic><topic>Matrix metalloproteinase</topic><topic>Matrix Metalloproteinase 7 - metabolism</topic><topic>Matrix metalloproteinases</topic><topic>Medical Biochemistry</topic><topic>Metastases</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Oncology</topic><topic>Peptides</topic><topic>Peptides - chemistry</topic><topic>Protein Binding</topic><topic>Proteolysis</topic><topic>T cells</topic><topic>T-Lymphocytes - cytology</topic><topic>T-Lymphocytes - immunology</topic><topic>T-Lymphocytes, Cytotoxic - immunology</topic><topic>Tumors</topic><topic>Vaccines</topic><topic>γ-Interferon</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yadav, Pavan Kumar</creatorcontrib><creatorcontrib>Gupta, Shishir Kumar</creatorcontrib><creatorcontrib>Kumar, Saroj</creatorcontrib><creatorcontrib>Ghosh, Mayukh</creatorcontrib><creatorcontrib>Yadav, Brijesh Singh</creatorcontrib><creatorcontrib>Kumar, Dinesh</creatorcontrib><creatorcontrib>Kumar, Ajay</creatorcontrib><creatorcontrib>Saini, Mohini</creatorcontrib><creatorcontrib>Kataria, Meena</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - 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Matrix metalloproteinases-7 (Matrilysin), the smallest member of the MMP family also performs quite alike; thus serves as a potential candidate for anti-tumor immunotherapy. Conversely, being an endogenous tumor-associated antigen (TAA), targeting MMP-7 for immunization is challenging. But MMP-7-based xenovaccine can surmount the obstacle of poor immunogenicity and immunological tolerance, often encountered in TAA-based conventional vaccine for anti-tumor immunotherapy. This paves the way for investigating the potential of MMP-7-derived major histocompatibility complex (MHC)-binding peptides to elicit precise epitope-specific T-cell responses towards their possible inclusion in anti-tumor vaccine formulations. Perhaps it also ushers the path of achieving multiple epitope-based broad and universal cellular immunity. In current experiment, an immunoinformatics approach has been employed to identify the putative canine matrix matelloproteinases-7 (cMMP-7)-derived peptides with MHC class-I-binding motifs which can elicit potent antigen-specific immune responses in BALB/c mice. Immunization with the cMMP-7 DNA vaccine induced a strong CD8
+
cytotoxic T lymphocytes (CTLs) and Th1- type response, with high level of gamma interferon (IFN-γ) production in BALB/c mice. The two identified putative MHC-I-binding nonameric peptides (Peptide
32-40
and Peptide
175-183
) from cMMP-7 induced significant lymphocyte proliferation along with the production of IFN-γ from CD8
+
T-cells in mice immunized with cMMP-7 DNA vaccine. The current observation has depicted the immunogenic potential of the two cMMP-7-derived nonapeptides for their possible exploitation in xenovaccine-mediated anti-tumor immunotherapy in mouse model.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>32970284</pmid><doi>10.1007/s11010-020-03908-2</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-1847-7486</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0300-8177 |
| ispartof | Molecular and cellular biochemistry, 2021-01, Vol.476 (1), p.311-320 |
| issn | 0300-8177 1573-4919 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7511522 |
| source | MEDLINE; SpringerNature Journals |
| subjects | Animals Antigen (tumor-associated) Antigenic determinants Antigens Antigens, Neoplasm - metabolism Binding Biochemistry Biomedical and Life Sciences Breast cancer Cancer Vaccines Cardiology CD8 antigen Cell Line, Tumor Cell Proliferation Cell-mediated immunity Computational Biology Cytotoxicity Deoxyribonucleic acid DNA DNA vaccines Dogs Epitopes Epitopes - chemistry Extracellular matrix Female HEK293 Cells Histocompatibility Antigens Class I - immunology Humans Immune response Immunization Immunogenicity Immunoglobulin G - immunology Immunological tolerance Immunology Immunotherapy Immunotherapy - methods Interferon Interferon-gamma Life Sciences Lymphocyte Activation Lymphocytes Lymphocytes T Major histocompatibility complex Mammary gland Mammary Glands, Animal - metabolism Matrilysin Matrix metalloproteinase Matrix Metalloproteinase 7 - metabolism Matrix metalloproteinases Medical Biochemistry Metastases Mice Mice, Inbred BALB C Oncology Peptides Peptides - chemistry Protein Binding Proteolysis T cells T-Lymphocytes - cytology T-Lymphocytes - immunology T-Lymphocytes, Cytotoxic - immunology Tumors Vaccines γ-Interferon |
| title | MMP-7 derived peptides with MHC class-I binding motifs from canine mammary tumor tissue elicit strong antigen-specific T-cell responses in BALB/c mice |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T22%3A53%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=MMP-7%20derived%20peptides%20with%20MHC%20class-I%20binding%20motifs%20from%20canine%20mammary%20tumor%20tissue%20elicit%20strong%20antigen-specific%20T-cell%20responses%20in%20BALB/c%20mice&rft.jtitle=Molecular%20and%20cellular%20biochemistry&rft.au=Yadav,%20Pavan%20Kumar&rft.date=2021-01-01&rft.volume=476&rft.issue=1&rft.spage=311&rft.epage=320&rft.pages=311-320&rft.issn=0300-8177&rft.eissn=1573-4919&rft_id=info:doi/10.1007/s11010-020-03908-2&rft_dat=%3Cgale_pubme%3EA651058465%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2486885407&rft_id=info:pmid/32970284&rft_galeid=A651058465&rfr_iscdi=true |