Dynamic Characterization of the Biomechanical Behaviour of Bovine Ovarian Cortical Tissue and Its Short-Term Effect on Ovarian Tissue and Follicles

The ovary is a dynamic mechanoresponsive organ. In vitro, tissue biomechanics was reported to affect follicle activation mainly through the Hippo pathway. Only recently, ovary responsiveness to mechanical signals was exploited for reproductive purposes. Unfortunately, poor characterization of ovaria...

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Veröffentlicht in:Materials 2020-08, Vol.13 (17), p.3759
Hauptverfasser: Pascoletti, Giulia, Di Nardo, Maddalena, Fragomeni, Gionata, Barbato, Vincenza, Capriglione, Teresa, Gualtieri, Roberto, Talevi, Riccardo, Catapano, Gerardo, Zanetti, Elisabetta M.
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container_issue 17
container_start_page 3759
container_title Materials
container_volume 13
creator Pascoletti, Giulia
Di Nardo, Maddalena
Fragomeni, Gionata
Barbato, Vincenza
Capriglione, Teresa
Gualtieri, Roberto
Talevi, Riccardo
Catapano, Gerardo
Zanetti, Elisabetta M.
description The ovary is a dynamic mechanoresponsive organ. In vitro, tissue biomechanics was reported to affect follicle activation mainly through the Hippo pathway. Only recently, ovary responsiveness to mechanical signals was exploited for reproductive purposes. Unfortunately, poor characterization of ovarian cortex biomechanics and of the mechanical challenge hampers reproducible and effective treatments, and prevention of tissue damages. In this study the biomechanical response of ovarian cortical tissue from abattoir bovines was characterized for the first time. Ovarian cortical tissue fragments were subjected to uniaxial dynamic testing at frequencies up to 30 Hz, and at increasing average stresses. Tissue structure prior to and after testing was characterized by histology, with established fixation and staining protocols, to assess follicle quality and stage. Tissue properties largely varied with the donor. Bovine ovarian cortical tissue consistently exhibited a nonlinear viscoelastic behavior, with dominant elastic characteristics, in the low range of other reproductive tissues, and significant creep. Strain rate was independent of the applied stress. Histological analysis prior to and after mechanical tests showed that the short-term dynamic mechanical test used for the study did not cause significant tissue tear, nor follicle expulsion or cell damage.
doi_str_mv 10.3390/ma13173759
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In vitro, tissue biomechanics was reported to affect follicle activation mainly through the Hippo pathway. Only recently, ovary responsiveness to mechanical signals was exploited for reproductive purposes. Unfortunately, poor characterization of ovarian cortex biomechanics and of the mechanical challenge hampers reproducible and effective treatments, and prevention of tissue damages. In this study the biomechanical response of ovarian cortical tissue from abattoir bovines was characterized for the first time. Ovarian cortical tissue fragments were subjected to uniaxial dynamic testing at frequencies up to 30 Hz, and at increasing average stresses. Tissue structure prior to and after testing was characterized by histology, with established fixation and staining protocols, to assess follicle quality and stage. Tissue properties largely varied with the donor. Bovine ovarian cortical tissue consistently exhibited a nonlinear viscoelastic behavior, with dominant elastic characteristics, in the low range of other reproductive tissues, and significant creep. Strain rate was independent of the applied stress. Histological analysis prior to and after mechanical tests showed that the short-term dynamic mechanical test used for the study did not cause significant tissue tear, nor follicle expulsion or cell damage.</description><identifier>ISSN: 1996-1944</identifier><identifier>EISSN: 1996-1944</identifier><identifier>DOI: 10.3390/ma13173759</identifier><identifier>PMID: 32854374</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>Abattoirs ; Biomechanics ; Damage prevention ; Dynamic tests ; Expulsion ; Extracellular matrix ; Histology ; Infertility ; Kinases ; Mechanical tests ; Ovaries ; Ovulation ; Patients ; Quality assessment ; Strain rate ; Transplants &amp; implants</subject><ispartof>Materials, 2020-08, Vol.13 (17), p.3759</ispartof><rights>2020. This work is licensed under http://creativecommons.org/licenses/by/3.0/ (the “License”). 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subjects Abattoirs
Biomechanics
Damage prevention
Dynamic tests
Expulsion
Extracellular matrix
Histology
Infertility
Kinases
Mechanical tests
Ovaries
Ovulation
Patients
Quality assessment
Strain rate
Transplants & implants
title Dynamic Characterization of the Biomechanical Behaviour of Bovine Ovarian Cortical Tissue and Its Short-Term Effect on Ovarian Tissue and Follicles
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