Dynamic Characterization of the Biomechanical Behaviour of Bovine Ovarian Cortical Tissue and Its Short-Term Effect on Ovarian Tissue and Follicles
The ovary is a dynamic mechanoresponsive organ. In vitro, tissue biomechanics was reported to affect follicle activation mainly through the Hippo pathway. Only recently, ovary responsiveness to mechanical signals was exploited for reproductive purposes. Unfortunately, poor characterization of ovaria...
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creator | Pascoletti, Giulia Di Nardo, Maddalena Fragomeni, Gionata Barbato, Vincenza Capriglione, Teresa Gualtieri, Roberto Talevi, Riccardo Catapano, Gerardo Zanetti, Elisabetta M. |
description | The ovary is a dynamic mechanoresponsive organ. In vitro, tissue biomechanics was reported to affect follicle activation mainly through the Hippo pathway. Only recently, ovary responsiveness to mechanical signals was exploited for reproductive purposes. Unfortunately, poor characterization of ovarian cortex biomechanics and of the mechanical challenge hampers reproducible and effective treatments, and prevention of tissue damages. In this study the biomechanical response of ovarian cortical tissue from abattoir bovines was characterized for the first time. Ovarian cortical tissue fragments were subjected to uniaxial dynamic testing at frequencies up to 30 Hz, and at increasing average stresses. Tissue structure prior to and after testing was characterized by histology, with established fixation and staining protocols, to assess follicle quality and stage. Tissue properties largely varied with the donor. Bovine ovarian cortical tissue consistently exhibited a nonlinear viscoelastic behavior, with dominant elastic characteristics, in the low range of other reproductive tissues, and significant creep. Strain rate was independent of the applied stress. Histological analysis prior to and after mechanical tests showed that the short-term dynamic mechanical test used for the study did not cause significant tissue tear, nor follicle expulsion or cell damage. |
doi_str_mv | 10.3390/ma13173759 |
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In vitro, tissue biomechanics was reported to affect follicle activation mainly through the Hippo pathway. Only recently, ovary responsiveness to mechanical signals was exploited for reproductive purposes. Unfortunately, poor characterization of ovarian cortex biomechanics and of the mechanical challenge hampers reproducible and effective treatments, and prevention of tissue damages. In this study the biomechanical response of ovarian cortical tissue from abattoir bovines was characterized for the first time. Ovarian cortical tissue fragments were subjected to uniaxial dynamic testing at frequencies up to 30 Hz, and at increasing average stresses. Tissue structure prior to and after testing was characterized by histology, with established fixation and staining protocols, to assess follicle quality and stage. Tissue properties largely varied with the donor. Bovine ovarian cortical tissue consistently exhibited a nonlinear viscoelastic behavior, with dominant elastic characteristics, in the low range of other reproductive tissues, and significant creep. Strain rate was independent of the applied stress. Histological analysis prior to and after mechanical tests showed that the short-term dynamic mechanical test used for the study did not cause significant tissue tear, nor follicle expulsion or cell damage.</description><identifier>ISSN: 1996-1944</identifier><identifier>EISSN: 1996-1944</identifier><identifier>DOI: 10.3390/ma13173759</identifier><identifier>PMID: 32854374</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>Abattoirs ; Biomechanics ; Damage prevention ; Dynamic tests ; Expulsion ; Extracellular matrix ; Histology ; Infertility ; Kinases ; Mechanical tests ; Ovaries ; Ovulation ; Patients ; Quality assessment ; Strain rate ; Transplants & implants</subject><ispartof>Materials, 2020-08, Vol.13 (17), p.3759</ispartof><rights>2020. This work is licensed under http://creativecommons.org/licenses/by/3.0/ (the “License”). 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In vitro, tissue biomechanics was reported to affect follicle activation mainly through the Hippo pathway. Only recently, ovary responsiveness to mechanical signals was exploited for reproductive purposes. Unfortunately, poor characterization of ovarian cortex biomechanics and of the mechanical challenge hampers reproducible and effective treatments, and prevention of tissue damages. In this study the biomechanical response of ovarian cortical tissue from abattoir bovines was characterized for the first time. Ovarian cortical tissue fragments were subjected to uniaxial dynamic testing at frequencies up to 30 Hz, and at increasing average stresses. Tissue structure prior to and after testing was characterized by histology, with established fixation and staining protocols, to assess follicle quality and stage. Tissue properties largely varied with the donor. Bovine ovarian cortical tissue consistently exhibited a nonlinear viscoelastic behavior, with dominant elastic characteristics, in the low range of other reproductive tissues, and significant creep. Strain rate was independent of the applied stress. Histological analysis prior to and after mechanical tests showed that the short-term dynamic mechanical test used for the study did not cause significant tissue tear, nor follicle expulsion or cell damage.</description><subject>Abattoirs</subject><subject>Biomechanics</subject><subject>Damage prevention</subject><subject>Dynamic tests</subject><subject>Expulsion</subject><subject>Extracellular matrix</subject><subject>Histology</subject><subject>Infertility</subject><subject>Kinases</subject><subject>Mechanical tests</subject><subject>Ovaries</subject><subject>Ovulation</subject><subject>Patients</subject><subject>Quality assessment</subject><subject>Strain rate</subject><subject>Transplants & implants</subject><issn>1996-1944</issn><issn>1996-1944</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNpdkctuFDEQRS0EIlHIhi-wxAYhdWg_-uENEjMkIVKkLDKsrRp3mXbUbQfbPVL4DX4Y5wF51MYl3eNbvi5C3rP6SAhVf56BCdaJrlGvyD5Tqq2YkvL1k36PHKZ0VZcSgvVcvSV7gveNFJ3cJ3--3XiYnaHrESKYjNH9huyCp8HSPCJduTCjGcE7AxNd4Qg7F5Z4K6_CznmkFzuIDjxdh5jvoI1LaUEKfqBnOdHLsQjVBuNMj61Fk2lx_3fpCXsSpsmZCdM78sbClPDw4TwgP06ON-vv1fnF6dn663llRC9yZRV2TT1ILlsuVCu3JRJyJbBT1gAOreVbzhpmOYCQLTDbq161tWoGZlqQ4oB8ufe9XrYzDgZ9jjDp6-hmiDc6gNPPFe9G_TPsdBkred0Xg48PBjH8WjBlPbtkcJrAY1iS5lL0bdfxXhX0wwv0qvyiL_HuqKbhsm4L9emeMjGkFNH-fwyr9e269eO6xV8OHpzF</recordid><startdate>20200825</startdate><enddate>20200825</enddate><creator>Pascoletti, Giulia</creator><creator>Di Nardo, Maddalena</creator><creator>Fragomeni, Gionata</creator><creator>Barbato, Vincenza</creator><creator>Capriglione, Teresa</creator><creator>Gualtieri, Roberto</creator><creator>Talevi, Riccardo</creator><creator>Catapano, Gerardo</creator><creator>Zanetti, Elisabetta M.</creator><general>MDPI AG</general><general>MDPI</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>KB.</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-4840-4165</orcidid><orcidid>https://orcid.org/0000-0002-6204-4020</orcidid><orcidid>https://orcid.org/0000-0003-4121-6126</orcidid></search><sort><creationdate>20200825</creationdate><title>Dynamic Characterization of the Biomechanical Behaviour of Bovine Ovarian Cortical Tissue and Its Short-Term Effect on Ovarian Tissue and Follicles</title><author>Pascoletti, Giulia ; Di Nardo, Maddalena ; Fragomeni, Gionata ; Barbato, Vincenza ; Capriglione, Teresa ; Gualtieri, Roberto ; Talevi, Riccardo ; Catapano, Gerardo ; Zanetti, Elisabetta M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c383t-f9e750d424623964b543e293e79fcaed6f2b2151f2aa346a1f89896095d1c6a43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Abattoirs</topic><topic>Biomechanics</topic><topic>Damage prevention</topic><topic>Dynamic tests</topic><topic>Expulsion</topic><topic>Extracellular matrix</topic><topic>Histology</topic><topic>Infertility</topic><topic>Kinases</topic><topic>Mechanical tests</topic><topic>Ovaries</topic><topic>Ovulation</topic><topic>Patients</topic><topic>Quality assessment</topic><topic>Strain rate</topic><topic>Transplants & implants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pascoletti, Giulia</creatorcontrib><creatorcontrib>Di Nardo, Maddalena</creatorcontrib><creatorcontrib>Fragomeni, Gionata</creatorcontrib><creatorcontrib>Barbato, Vincenza</creatorcontrib><creatorcontrib>Capriglione, Teresa</creatorcontrib><creatorcontrib>Gualtieri, Roberto</creatorcontrib><creatorcontrib>Talevi, Riccardo</creatorcontrib><creatorcontrib>Catapano, Gerardo</creatorcontrib><creatorcontrib>Zanetti, Elisabetta M.</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>Materials Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Materials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pascoletti, Giulia</au><au>Di Nardo, Maddalena</au><au>Fragomeni, Gionata</au><au>Barbato, Vincenza</au><au>Capriglione, Teresa</au><au>Gualtieri, Roberto</au><au>Talevi, Riccardo</au><au>Catapano, Gerardo</au><au>Zanetti, Elisabetta M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dynamic Characterization of the Biomechanical Behaviour of Bovine Ovarian Cortical Tissue and Its Short-Term Effect on Ovarian Tissue and Follicles</atitle><jtitle>Materials</jtitle><date>2020-08-25</date><risdate>2020</risdate><volume>13</volume><issue>17</issue><spage>3759</spage><pages>3759-</pages><issn>1996-1944</issn><eissn>1996-1944</eissn><abstract>The ovary is a dynamic mechanoresponsive organ. In vitro, tissue biomechanics was reported to affect follicle activation mainly through the Hippo pathway. Only recently, ovary responsiveness to mechanical signals was exploited for reproductive purposes. Unfortunately, poor characterization of ovarian cortex biomechanics and of the mechanical challenge hampers reproducible and effective treatments, and prevention of tissue damages. In this study the biomechanical response of ovarian cortical tissue from abattoir bovines was characterized for the first time. Ovarian cortical tissue fragments were subjected to uniaxial dynamic testing at frequencies up to 30 Hz, and at increasing average stresses. Tissue structure prior to and after testing was characterized by histology, with established fixation and staining protocols, to assess follicle quality and stage. Tissue properties largely varied with the donor. Bovine ovarian cortical tissue consistently exhibited a nonlinear viscoelastic behavior, with dominant elastic characteristics, in the low range of other reproductive tissues, and significant creep. Strain rate was independent of the applied stress. Histological analysis prior to and after mechanical tests showed that the short-term dynamic mechanical test used for the study did not cause significant tissue tear, nor follicle expulsion or cell damage.</abstract><cop>Basel</cop><pub>MDPI AG</pub><pmid>32854374</pmid><doi>10.3390/ma13173759</doi><orcidid>https://orcid.org/0000-0002-4840-4165</orcidid><orcidid>https://orcid.org/0000-0002-6204-4020</orcidid><orcidid>https://orcid.org/0000-0003-4121-6126</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Abattoirs Biomechanics Damage prevention Dynamic tests Expulsion Extracellular matrix Histology Infertility Kinases Mechanical tests Ovaries Ovulation Patients Quality assessment Strain rate Transplants & implants |
title | Dynamic Characterization of the Biomechanical Behaviour of Bovine Ovarian Cortical Tissue and Its Short-Term Effect on Ovarian Tissue and Follicles |
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