The Role of Amino Acids in Neurotransmission and Fluorescent Tools for Their Detection
Neurotransmission between neurons, which can occur over the span of a few milliseconds, relies on the controlled release of small molecule neurotransmitters, many of which are amino acids. Fluorescence imaging provides the necessary speed to follow these events and has emerged as a powerful techniqu...
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| Veröffentlicht in: | International journal of molecular sciences 2020-08, Vol.21 (17), p.6197 |
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| creator | Dalangin, Rochelin Kim, Anna Campbell, Robert E |
| description | Neurotransmission between neurons, which can occur over the span of a few milliseconds, relies on the controlled release of small molecule neurotransmitters, many of which are amino acids. Fluorescence imaging provides the necessary speed to follow these events and has emerged as a powerful technique for investigating neurotransmission. In this review, we highlight some of the roles of the 20 canonical amino acids, GABA and β-alanine in neurotransmission. We also discuss available fluorescence-based probes for amino acids that have been shown to be compatible for live cell imaging, namely those based on synthetic dyes, nanostructures (quantum dots and nanotubes), and genetically encoded components. We aim to provide tool developers with information that may guide future engineering efforts and tool users with information regarding existing indicators to facilitate studies of amino acid dynamics. |
| doi_str_mv | 10.3390/ijms21176197 |
| format | Article |
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Fluorescence imaging provides the necessary speed to follow these events and has emerged as a powerful technique for investigating neurotransmission. In this review, we highlight some of the roles of the 20 canonical amino acids, GABA and β-alanine in neurotransmission. We also discuss available fluorescence-based probes for amino acids that have been shown to be compatible for live cell imaging, namely those based on synthetic dyes, nanostructures (quantum dots and nanotubes), and genetically encoded components. 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| subjects | Alanine Amino acids Amino Acids - analysis Amino Acids - metabolism Animals beta-Alanine - analysis beta-Alanine - metabolism Biosensing Techniques Brain Controlled release Fluorescence Fluorescent Dyes - chemistry Fluorescent indicators gamma-Aminobutyric Acid - analysis gamma-Aminobutyric Acid - metabolism Gene expression Genetic code Humans Metabolites Nervous system Neurotransmission Neurotransmitters Optical Imaging Physiology Quantum dots Review Roles Synaptic Transmission γ-Aminobutyric acid |
| title | The Role of Amino Acids in Neurotransmission and Fluorescent Tools for Their Detection |
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