Critical Involvement of Calcium-Dependent Cytosolic Phospholipase A2α in Aortic Valve Interstitial Cell Calcification
The involvement of calcium-dependent cytosolic phospholipase A2α (cPLA2α) in aortic valve calcification is not exhaustively elucidated. Here, cPLA2α expression in aortic valve interstitial cell (AVIC) pro-calcific cultures simulating either metastatic or dystrophic calcification was estimated by qPC...
Gespeichert in:
| Veröffentlicht in: | International journal of molecular sciences 2020-09, Vol.21 (17), p.6398 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 17 |
| container_start_page | 6398 |
| container_title | International journal of molecular sciences |
| container_volume | 21 |
| creator | Bonetti, Antonella Allegri, Lorenzo Baldan, Federica Contin, Magali Battistella, Claudio Damante, Giuseppe Marchini, Maurizio Ortolani, Fulvia |
| description | The involvement of calcium-dependent cytosolic phospholipase A2α (cPLA2α) in aortic valve calcification is not exhaustively elucidated. Here, cPLA2α expression in aortic valve interstitial cell (AVIC) pro-calcific cultures simulating either metastatic or dystrophic calcification was estimated by qPCR, Western blotting, and counting of cPLA2α-immunoreactive cells, with parallel ultrastructural examination of AVIC calcific degeneration. These evaluations also involved pro-calcific AVIC cultures treated with cPLA2α inhibitor dexamethasone. cPLA2α over-expression resulted for both types of pro-calcific AVIC cultures. Compared to controls, enzyme content was found to increase by up to 300% and 186% in metastatic and dystrophic calcification-like cultures, respectively. Increases in mRNA amounts were also observed, although they were not as striking as those in enzyme content. Moreover, cPLA2α increases were time-dependent and strictly associated with mineralization progression. Conversely, drastically lower levels of enzyme content resulted for the pro-calcific AVIC cultures supplemented with dexamethasone. In particular, cPLA2α amounts were found to decrease by almost 88% and 48% in metastatic and dystrophic calcification-like cultures, respectively, with mRNA amounts showing a similar trend. Interestingly, these drastic decreases in cPLA2α amounts were paralleled by drastic decreases in mineralization degrees, as revealed ultrastructurally. In conclusion, cPLA2α may be regarded as a crucial co-factor contributing to AVIC mineralization in vitro, thus being an attractive potential target for designing novel therapeutic strategies aimed to counteract onset or progression of calcific aortic valve diseases. |
| doi_str_mv | 10.3390/ijms21176398 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7503521</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2441279542</sourcerecordid><originalsourceid>FETCH-LOGICAL-c412t-4e15878572b911440a88e83d00d1f8f09b1faf8eeb14f5c7f9fd195ab348b7c63</originalsourceid><addsrcrecordid>eNpdkc1q3DAYRUVJ6KRpd10HQzZZ1Il-R9ImMLg_CQTSRdutkGUpo8G2HMkeyGPlRfJM0TDTYZKVPqTD0b18AHxF8JIQCa_8qksYIT4nUnwAJ4hiXEI450cH8wx8SmkFISaYyY9gRrCQEmF2AtZV9KM3ui1u-3Vo17az_VgEV1S6NX7qyu92sH2zuayexpBC603xexnSsMzjoJMtFvjlufB9sQgxm4p_OluybbQxjdmd1ZVt263Q5a9GH_rP4NjpNtkvu_MU_P354091U97d_7qtFneloQiPJbWICS4Yx7VEiFKohbCCNBA2yAkHZY2cdsLaGlHHDHfSNUgyXRMqam7m5BRcb73DVHe2MblH1K0aou90fFJBe_X2pfdL9RDWijNIGEZZcLETxPA42TSqzieT--jehikpTHNQLhnFGT1_h67CFPtcb0NBziThm0TftpSJIaVo3T4MgmqzUHW40IyfHRbYw_83SF4BEn6e1A</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2440759376</pqid></control><display><type>article</type><title>Critical Involvement of Calcium-Dependent Cytosolic Phospholipase A2α in Aortic Valve Interstitial Cell Calcification</title><source>MDPI - Multidisciplinary Digital Publishing Institute</source><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><creator>Bonetti, Antonella ; Allegri, Lorenzo ; Baldan, Federica ; Contin, Magali ; Battistella, Claudio ; Damante, Giuseppe ; Marchini, Maurizio ; Ortolani, Fulvia</creator><creatorcontrib>Bonetti, Antonella ; Allegri, Lorenzo ; Baldan, Federica ; Contin, Magali ; Battistella, Claudio ; Damante, Giuseppe ; Marchini, Maurizio ; Ortolani, Fulvia</creatorcontrib><description>The involvement of calcium-dependent cytosolic phospholipase A2α (cPLA2α) in aortic valve calcification is not exhaustively elucidated. Here, cPLA2α expression in aortic valve interstitial cell (AVIC) pro-calcific cultures simulating either metastatic or dystrophic calcification was estimated by qPCR, Western blotting, and counting of cPLA2α-immunoreactive cells, with parallel ultrastructural examination of AVIC calcific degeneration. These evaluations also involved pro-calcific AVIC cultures treated with cPLA2α inhibitor dexamethasone. cPLA2α over-expression resulted for both types of pro-calcific AVIC cultures. Compared to controls, enzyme content was found to increase by up to 300% and 186% in metastatic and dystrophic calcification-like cultures, respectively. Increases in mRNA amounts were also observed, although they were not as striking as those in enzyme content. Moreover, cPLA2α increases were time-dependent and strictly associated with mineralization progression. Conversely, drastically lower levels of enzyme content resulted for the pro-calcific AVIC cultures supplemented with dexamethasone. In particular, cPLA2α amounts were found to decrease by almost 88% and 48% in metastatic and dystrophic calcification-like cultures, respectively, with mRNA amounts showing a similar trend. Interestingly, these drastic decreases in cPLA2α amounts were paralleled by drastic decreases in mineralization degrees, as revealed ultrastructurally. In conclusion, cPLA2α may be regarded as a crucial co-factor contributing to AVIC mineralization in vitro, thus being an attractive potential target for designing novel therapeutic strategies aimed to counteract onset or progression of calcific aortic valve diseases.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms21176398</identifier><identifier>PMID: 32899125</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Animals ; Aorta ; Aortic valve ; Aortic Valve - metabolism ; Aortic Valve - pathology ; Aortic Valve Stenosis - metabolism ; Aortic Valve Stenosis - pathology ; Calcification ; Calcification (ectopic) ; Calcinosis - metabolism ; Calcinosis - pathology ; Calcium ; Calcium - metabolism ; Cattle ; Cells, Cultured ; Degeneration ; Dexamethasone ; Enzymes ; Gene expression ; Group IV Phospholipases A2 - genetics ; Group IV Phospholipases A2 - metabolism ; Heart valves ; Interstitial Cells of Cajal - metabolism ; Interstitial Cells of Cajal - pathology ; Lipids ; Metastases ; Metastasis ; Mineralization ; mRNA ; Overexpression ; Phosphatase ; Phospholipase ; Phospholipase A2 ; Steroids ; Western blotting</subject><ispartof>International journal of molecular sciences, 2020-09, Vol.21 (17), p.6398</ispartof><rights>2020. This work is licensed under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 by the authors. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-4e15878572b911440a88e83d00d1f8f09b1faf8eeb14f5c7f9fd195ab348b7c63</citedby><cites>FETCH-LOGICAL-c412t-4e15878572b911440a88e83d00d1f8f09b1faf8eeb14f5c7f9fd195ab348b7c63</cites><orcidid>0000-0001-5028-8135 ; 0000-0001-5268-7201 ; 0000-0003-1164-1316</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7503521/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7503521/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32899125$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bonetti, Antonella</creatorcontrib><creatorcontrib>Allegri, Lorenzo</creatorcontrib><creatorcontrib>Baldan, Federica</creatorcontrib><creatorcontrib>Contin, Magali</creatorcontrib><creatorcontrib>Battistella, Claudio</creatorcontrib><creatorcontrib>Damante, Giuseppe</creatorcontrib><creatorcontrib>Marchini, Maurizio</creatorcontrib><creatorcontrib>Ortolani, Fulvia</creatorcontrib><title>Critical Involvement of Calcium-Dependent Cytosolic Phospholipase A2α in Aortic Valve Interstitial Cell Calcification</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>The involvement of calcium-dependent cytosolic phospholipase A2α (cPLA2α) in aortic valve calcification is not exhaustively elucidated. Here, cPLA2α expression in aortic valve interstitial cell (AVIC) pro-calcific cultures simulating either metastatic or dystrophic calcification was estimated by qPCR, Western blotting, and counting of cPLA2α-immunoreactive cells, with parallel ultrastructural examination of AVIC calcific degeneration. These evaluations also involved pro-calcific AVIC cultures treated with cPLA2α inhibitor dexamethasone. cPLA2α over-expression resulted for both types of pro-calcific AVIC cultures. Compared to controls, enzyme content was found to increase by up to 300% and 186% in metastatic and dystrophic calcification-like cultures, respectively. Increases in mRNA amounts were also observed, although they were not as striking as those in enzyme content. Moreover, cPLA2α increases were time-dependent and strictly associated with mineralization progression. Conversely, drastically lower levels of enzyme content resulted for the pro-calcific AVIC cultures supplemented with dexamethasone. In particular, cPLA2α amounts were found to decrease by almost 88% and 48% in metastatic and dystrophic calcification-like cultures, respectively, with mRNA amounts showing a similar trend. Interestingly, these drastic decreases in cPLA2α amounts were paralleled by drastic decreases in mineralization degrees, as revealed ultrastructurally. In conclusion, cPLA2α may be regarded as a crucial co-factor contributing to AVIC mineralization in vitro, thus being an attractive potential target for designing novel therapeutic strategies aimed to counteract onset or progression of calcific aortic valve diseases.</description><subject>Animals</subject><subject>Aorta</subject><subject>Aortic valve</subject><subject>Aortic Valve - metabolism</subject><subject>Aortic Valve - pathology</subject><subject>Aortic Valve Stenosis - metabolism</subject><subject>Aortic Valve Stenosis - pathology</subject><subject>Calcification</subject><subject>Calcification (ectopic)</subject><subject>Calcinosis - metabolism</subject><subject>Calcinosis - pathology</subject><subject>Calcium</subject><subject>Calcium - metabolism</subject><subject>Cattle</subject><subject>Cells, Cultured</subject><subject>Degeneration</subject><subject>Dexamethasone</subject><subject>Enzymes</subject><subject>Gene expression</subject><subject>Group IV Phospholipases A2 - genetics</subject><subject>Group IV Phospholipases A2 - metabolism</subject><subject>Heart valves</subject><subject>Interstitial Cells of Cajal - metabolism</subject><subject>Interstitial Cells of Cajal - pathology</subject><subject>Lipids</subject><subject>Metastases</subject><subject>Metastasis</subject><subject>Mineralization</subject><subject>mRNA</subject><subject>Overexpression</subject><subject>Phosphatase</subject><subject>Phospholipase</subject><subject>Phospholipase A2</subject><subject>Steroids</subject><subject>Western blotting</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkc1q3DAYRUVJ6KRpd10HQzZZ1Il-R9ImMLg_CQTSRdutkGUpo8G2HMkeyGPlRfJM0TDTYZKVPqTD0b18AHxF8JIQCa_8qksYIT4nUnwAJ4hiXEI450cH8wx8SmkFISaYyY9gRrCQEmF2AtZV9KM3ui1u-3Vo17az_VgEV1S6NX7qyu92sH2zuayexpBC603xexnSsMzjoJMtFvjlufB9sQgxm4p_OluybbQxjdmd1ZVt263Q5a9GH_rP4NjpNtkvu_MU_P354091U97d_7qtFneloQiPJbWICS4Yx7VEiFKohbCCNBA2yAkHZY2cdsLaGlHHDHfSNUgyXRMqam7m5BRcb73DVHe2MblH1K0aou90fFJBe_X2pfdL9RDWijNIGEZZcLETxPA42TSqzieT--jehikpTHNQLhnFGT1_h67CFPtcb0NBziThm0TftpSJIaVo3T4MgmqzUHW40IyfHRbYw_83SF4BEn6e1A</recordid><startdate>20200903</startdate><enddate>20200903</enddate><creator>Bonetti, Antonella</creator><creator>Allegri, Lorenzo</creator><creator>Baldan, Federica</creator><creator>Contin, Magali</creator><creator>Battistella, Claudio</creator><creator>Damante, Giuseppe</creator><creator>Marchini, Maurizio</creator><creator>Ortolani, Fulvia</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-5028-8135</orcidid><orcidid>https://orcid.org/0000-0001-5268-7201</orcidid><orcidid>https://orcid.org/0000-0003-1164-1316</orcidid></search><sort><creationdate>20200903</creationdate><title>Critical Involvement of Calcium-Dependent Cytosolic Phospholipase A2α in Aortic Valve Interstitial Cell Calcification</title><author>Bonetti, Antonella ; Allegri, Lorenzo ; Baldan, Federica ; Contin, Magali ; Battistella, Claudio ; Damante, Giuseppe ; Marchini, Maurizio ; Ortolani, Fulvia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-4e15878572b911440a88e83d00d1f8f09b1faf8eeb14f5c7f9fd195ab348b7c63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Aorta</topic><topic>Aortic valve</topic><topic>Aortic Valve - metabolism</topic><topic>Aortic Valve - pathology</topic><topic>Aortic Valve Stenosis - metabolism</topic><topic>Aortic Valve Stenosis - pathology</topic><topic>Calcification</topic><topic>Calcification (ectopic)</topic><topic>Calcinosis - metabolism</topic><topic>Calcinosis - pathology</topic><topic>Calcium</topic><topic>Calcium - metabolism</topic><topic>Cattle</topic><topic>Cells, Cultured</topic><topic>Degeneration</topic><topic>Dexamethasone</topic><topic>Enzymes</topic><topic>Gene expression</topic><topic>Group IV Phospholipases A2 - genetics</topic><topic>Group IV Phospholipases A2 - metabolism</topic><topic>Heart valves</topic><topic>Interstitial Cells of Cajal - metabolism</topic><topic>Interstitial Cells of Cajal - pathology</topic><topic>Lipids</topic><topic>Metastases</topic><topic>Metastasis</topic><topic>Mineralization</topic><topic>mRNA</topic><topic>Overexpression</topic><topic>Phosphatase</topic><topic>Phospholipase</topic><topic>Phospholipase A2</topic><topic>Steroids</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bonetti, Antonella</creatorcontrib><creatorcontrib>Allegri, Lorenzo</creatorcontrib><creatorcontrib>Baldan, Federica</creatorcontrib><creatorcontrib>Contin, Magali</creatorcontrib><creatorcontrib>Battistella, Claudio</creatorcontrib><creatorcontrib>Damante, Giuseppe</creatorcontrib><creatorcontrib>Marchini, Maurizio</creatorcontrib><creatorcontrib>Ortolani, Fulvia</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bonetti, Antonella</au><au>Allegri, Lorenzo</au><au>Baldan, Federica</au><au>Contin, Magali</au><au>Battistella, Claudio</au><au>Damante, Giuseppe</au><au>Marchini, Maurizio</au><au>Ortolani, Fulvia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Critical Involvement of Calcium-Dependent Cytosolic Phospholipase A2α in Aortic Valve Interstitial Cell Calcification</atitle><jtitle>International journal of molecular sciences</jtitle><addtitle>Int J Mol Sci</addtitle><date>2020-09-03</date><risdate>2020</risdate><volume>21</volume><issue>17</issue><spage>6398</spage><pages>6398-</pages><issn>1422-0067</issn><issn>1661-6596</issn><eissn>1422-0067</eissn><abstract>The involvement of calcium-dependent cytosolic phospholipase A2α (cPLA2α) in aortic valve calcification is not exhaustively elucidated. Here, cPLA2α expression in aortic valve interstitial cell (AVIC) pro-calcific cultures simulating either metastatic or dystrophic calcification was estimated by qPCR, Western blotting, and counting of cPLA2α-immunoreactive cells, with parallel ultrastructural examination of AVIC calcific degeneration. These evaluations also involved pro-calcific AVIC cultures treated with cPLA2α inhibitor dexamethasone. cPLA2α over-expression resulted for both types of pro-calcific AVIC cultures. Compared to controls, enzyme content was found to increase by up to 300% and 186% in metastatic and dystrophic calcification-like cultures, respectively. Increases in mRNA amounts were also observed, although they were not as striking as those in enzyme content. Moreover, cPLA2α increases were time-dependent and strictly associated with mineralization progression. Conversely, drastically lower levels of enzyme content resulted for the pro-calcific AVIC cultures supplemented with dexamethasone. In particular, cPLA2α amounts were found to decrease by almost 88% and 48% in metastatic and dystrophic calcification-like cultures, respectively, with mRNA amounts showing a similar trend. Interestingly, these drastic decreases in cPLA2α amounts were paralleled by drastic decreases in mineralization degrees, as revealed ultrastructurally. In conclusion, cPLA2α may be regarded as a crucial co-factor contributing to AVIC mineralization in vitro, thus being an attractive potential target for designing novel therapeutic strategies aimed to counteract onset or progression of calcific aortic valve diseases.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>32899125</pmid><doi>10.3390/ijms21176398</doi><orcidid>https://orcid.org/0000-0001-5028-8135</orcidid><orcidid>https://orcid.org/0000-0001-5268-7201</orcidid><orcidid>https://orcid.org/0000-0003-1164-1316</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1422-0067 |
| ispartof | International journal of molecular sciences, 2020-09, Vol.21 (17), p.6398 |
| issn | 1422-0067 1661-6596 1422-0067 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7503521 |
| source | MDPI - Multidisciplinary Digital Publishing Institute; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Animals Aorta Aortic valve Aortic Valve - metabolism Aortic Valve - pathology Aortic Valve Stenosis - metabolism Aortic Valve Stenosis - pathology Calcification Calcification (ectopic) Calcinosis - metabolism Calcinosis - pathology Calcium Calcium - metabolism Cattle Cells, Cultured Degeneration Dexamethasone Enzymes Gene expression Group IV Phospholipases A2 - genetics Group IV Phospholipases A2 - metabolism Heart valves Interstitial Cells of Cajal - metabolism Interstitial Cells of Cajal - pathology Lipids Metastases Metastasis Mineralization mRNA Overexpression Phosphatase Phospholipase Phospholipase A2 Steroids Western blotting |
| title | Critical Involvement of Calcium-Dependent Cytosolic Phospholipase A2α in Aortic Valve Interstitial Cell Calcification |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-08T05%3A09%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Critical%20Involvement%20of%20Calcium-Dependent%20Cytosolic%20Phospholipase%20A2%CE%B1%20in%20Aortic%20Valve%20Interstitial%20Cell%20Calcification&rft.jtitle=International%20journal%20of%20molecular%20sciences&rft.au=Bonetti,%20Antonella&rft.date=2020-09-03&rft.volume=21&rft.issue=17&rft.spage=6398&rft.pages=6398-&rft.issn=1422-0067&rft.eissn=1422-0067&rft_id=info:doi/10.3390/ijms21176398&rft_dat=%3Cproquest_pubme%3E2441279542%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2440759376&rft_id=info:pmid/32899125&rfr_iscdi=true |