Detection of SARS-CoV-2 in conjunctival secretions from patients without ocular symptoms
Purpose To evaluate the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in conjunctival secretions from patients without ocular symptoms. Methods Conjunctival swabs were prospectively collected from laboratory-confirmed Coronavirus disease 2019 (COVID-19) patients without oc...
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| Veröffentlicht in: | Infection 2021-04, Vol.49 (2), p.257-265 |
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| creator | Li, Xin Chan, Jasper Fuk-Woo Li, Kenneth Kai-Wang Tso, Eugene Yuk-Keung Yip, Cyril Chik-Yan Sridhar, Siddharth Chung, Tom Wai-Hin Chiu, Kelvin Hei-Yeung Hung, Derek Ling-Lung Wu, Alan Ka-Lun Chau, Sandy Ka-Yee Liu, Raymond Lung, Kwok-Cheung Tam, Anthony Raymond Cheng, Vincent Chi-Chung To, Kelvin Kai-Wang Chan, Kwok-Hung Hung, Ivan Fan-Ngai Yuen, Kwok-Yung |
| description | Purpose
To evaluate the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in conjunctival secretions from patients without ocular symptoms.
Methods
Conjunctival swabs were prospectively collected from laboratory-confirmed Coronavirus disease 2019 (COVID-19) patients without ocular symptoms for reverse transcription-polymerase chain reaction (RT-PCR) and viral culture.
Results
A total of 158 conjunctival swabs were obtained from 49 laboratory-confirmed COVID-19 patients. The median duration of illness when the first conjunctival swab was obtained was 10 days (range 2–27 days). Four conjunctival swabs from four different patients (4/49, 8.2%) were positive for SARS-CoV-2 RNA by RT-PCR. The Ct values ranged from 32.7 to 37.7 (mean 35.4). Viral cultures were negative for all four RT-PCR-positive conjunctival swabs.
Conclusion
Conjunctival secretions of a minority of COVID-19 patients without ocular symptoms may contain relatively low levels of SARS-CoV-2 RNA, but their infectiousness remains undetermined. Appropriate infection control measures should be implemented during ophthalmological assessment of COVID-19 patients to prevent potential nosocomial transmission of SARS-CoV-2. |
| doi_str_mv | 10.1007/s15010-020-01524-2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7497856</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2504631389</sourcerecordid><originalsourceid>FETCH-LOGICAL-c474t-f04e709b155fd164f872f6c0eaa6e38c58456864059bbacab1ce051bc2c1ac003</originalsourceid><addsrcrecordid>eNp9kU9rFTEUxYMo9ln9Ai4k4MZN6k1yM5PZCOVZrVAoWBV3IZNm2nnMJM8k09Jvb-qr9c-ii3AX53dPzuUQ8pLDAQdo32augAMDUR9XApl4RFYcZcega-VjsgIJwDQXzR55lvMGAFSH7VOyJ0WHqBFW5Pt7X7wrYww0DvTs8PMZW8dvTNAxUBfDZglVvLITzd4lf8tlOqQ4060tow8l0-uxXMal0OiWySaab-ZtiXN-Tp4Mdsr-xd3cJ18_HH1ZH7OT04-f1ocnzGGLhQ2AvoWu50oN57zBQbdiaBx4axsvtVMaVaMbrMn73jrbc-dB8d4Jx60DkPvk3c53u_SzP3c1U7KT2aZxtunGRDuaf5UwXpqLeGVa7Fqtmmrw5s4gxR-Lz8XMY3Z-mmzwcclGIKLUQnZY0df_oZu4pFDPM0IBNpJL3VVK7CiXYs7JD_dhOJjb4syuOFOLM7-KM6Iuvfr7jPuV301VQO6AXKVw4dOfvx-w_Qksh6SG</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2504631389</pqid></control><display><type>article</type><title>Detection of SARS-CoV-2 in conjunctival secretions from patients without ocular symptoms</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Li, Xin ; Chan, Jasper Fuk-Woo ; Li, Kenneth Kai-Wang ; Tso, Eugene Yuk-Keung ; Yip, Cyril Chik-Yan ; Sridhar, Siddharth ; Chung, Tom Wai-Hin ; Chiu, Kelvin Hei-Yeung ; Hung, Derek Ling-Lung ; Wu, Alan Ka-Lun ; Chau, Sandy Ka-Yee ; Liu, Raymond ; Lung, Kwok-Cheung ; Tam, Anthony Raymond ; Cheng, Vincent Chi-Chung ; To, Kelvin Kai-Wang ; Chan, Kwok-Hung ; Hung, Ivan Fan-Ngai ; Yuen, Kwok-Yung</creator><creatorcontrib>Li, Xin ; Chan, Jasper Fuk-Woo ; Li, Kenneth Kai-Wang ; Tso, Eugene Yuk-Keung ; Yip, Cyril Chik-Yan ; Sridhar, Siddharth ; Chung, Tom Wai-Hin ; Chiu, Kelvin Hei-Yeung ; Hung, Derek Ling-Lung ; Wu, Alan Ka-Lun ; Chau, Sandy Ka-Yee ; Liu, Raymond ; Lung, Kwok-Cheung ; Tam, Anthony Raymond ; Cheng, Vincent Chi-Chung ; To, Kelvin Kai-Wang ; Chan, Kwok-Hung ; Hung, Ivan Fan-Ngai ; Yuen, Kwok-Yung</creatorcontrib><description>Purpose
To evaluate the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in conjunctival secretions from patients without ocular symptoms.
Methods
Conjunctival swabs were prospectively collected from laboratory-confirmed Coronavirus disease 2019 (COVID-19) patients without ocular symptoms for reverse transcription-polymerase chain reaction (RT-PCR) and viral culture.
Results
A total of 158 conjunctival swabs were obtained from 49 laboratory-confirmed COVID-19 patients. The median duration of illness when the first conjunctival swab was obtained was 10 days (range 2–27 days). Four conjunctival swabs from four different patients (4/49, 8.2%) were positive for SARS-CoV-2 RNA by RT-PCR. The Ct values ranged from 32.7 to 37.7 (mean 35.4). Viral cultures were negative for all four RT-PCR-positive conjunctival swabs.
Conclusion
Conjunctival secretions of a minority of COVID-19 patients without ocular symptoms may contain relatively low levels of SARS-CoV-2 RNA, but their infectiousness remains undetermined. Appropriate infection control measures should be implemented during ophthalmological assessment of COVID-19 patients to prevent potential nosocomial transmission of SARS-CoV-2.</description><identifier>ISSN: 0300-8126</identifier><identifier>EISSN: 1439-0973</identifier><identifier>DOI: 10.1007/s15010-020-01524-2</identifier><identifier>PMID: 32944840</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Animals ; Chlorocebus aethiops ; Conjunctiva - virology ; Coronaviruses ; COVID-19 ; COVID-19 - diagnosis ; COVID-19 - virology ; COVID-19 Nucleic Acid Testing ; Disease transmission ; Family Medicine ; Female ; General Practice ; Hospitals ; Humans ; Infectious Diseases ; Internal Medicine ; Laboratories ; Male ; Medicine ; Medicine & Public Health ; Middle Aged ; Nosocomial infection ; Original Paper ; Polymerase chain reaction ; Reverse transcription ; RNA, Viral - genetics ; RNA, Viral - isolation & purification ; SARS-CoV-2 ; SARS-CoV-2 - genetics ; SARS-CoV-2 - growth & development ; SARS-CoV-2 - isolation & purification ; Secretions ; Severe acute respiratory syndrome coronavirus 2 ; Signs and symptoms ; Vero Cells ; Viral diseases ; Virus Shedding ; Young Adult</subject><ispartof>Infection, 2021-04, Vol.49 (2), p.257-265</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020</rights><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-f04e709b155fd164f872f6c0eaa6e38c58456864059bbacab1ce051bc2c1ac003</citedby><cites>FETCH-LOGICAL-c474t-f04e709b155fd164f872f6c0eaa6e38c58456864059bbacab1ce051bc2c1ac003</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s15010-020-01524-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s15010-020-01524-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32944840$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Xin</creatorcontrib><creatorcontrib>Chan, Jasper Fuk-Woo</creatorcontrib><creatorcontrib>Li, Kenneth Kai-Wang</creatorcontrib><creatorcontrib>Tso, Eugene Yuk-Keung</creatorcontrib><creatorcontrib>Yip, Cyril Chik-Yan</creatorcontrib><creatorcontrib>Sridhar, Siddharth</creatorcontrib><creatorcontrib>Chung, Tom Wai-Hin</creatorcontrib><creatorcontrib>Chiu, Kelvin Hei-Yeung</creatorcontrib><creatorcontrib>Hung, Derek Ling-Lung</creatorcontrib><creatorcontrib>Wu, Alan Ka-Lun</creatorcontrib><creatorcontrib>Chau, Sandy Ka-Yee</creatorcontrib><creatorcontrib>Liu, Raymond</creatorcontrib><creatorcontrib>Lung, Kwok-Cheung</creatorcontrib><creatorcontrib>Tam, Anthony Raymond</creatorcontrib><creatorcontrib>Cheng, Vincent Chi-Chung</creatorcontrib><creatorcontrib>To, Kelvin Kai-Wang</creatorcontrib><creatorcontrib>Chan, Kwok-Hung</creatorcontrib><creatorcontrib>Hung, Ivan Fan-Ngai</creatorcontrib><creatorcontrib>Yuen, Kwok-Yung</creatorcontrib><title>Detection of SARS-CoV-2 in conjunctival secretions from patients without ocular symptoms</title><title>Infection</title><addtitle>Infection</addtitle><addtitle>Infection</addtitle><description>Purpose
To evaluate the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in conjunctival secretions from patients without ocular symptoms.
Methods
Conjunctival swabs were prospectively collected from laboratory-confirmed Coronavirus disease 2019 (COVID-19) patients without ocular symptoms for reverse transcription-polymerase chain reaction (RT-PCR) and viral culture.
Results
A total of 158 conjunctival swabs were obtained from 49 laboratory-confirmed COVID-19 patients. The median duration of illness when the first conjunctival swab was obtained was 10 days (range 2–27 days). Four conjunctival swabs from four different patients (4/49, 8.2%) were positive for SARS-CoV-2 RNA by RT-PCR. The Ct values ranged from 32.7 to 37.7 (mean 35.4). Viral cultures were negative for all four RT-PCR-positive conjunctival swabs.
Conclusion
Conjunctival secretions of a minority of COVID-19 patients without ocular symptoms may contain relatively low levels of SARS-CoV-2 RNA, but their infectiousness remains undetermined. Appropriate infection control measures should be implemented during ophthalmological assessment of COVID-19 patients to prevent potential nosocomial transmission of SARS-CoV-2.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Animals</subject><subject>Chlorocebus aethiops</subject><subject>Conjunctiva - virology</subject><subject>Coronaviruses</subject><subject>COVID-19</subject><subject>COVID-19 - diagnosis</subject><subject>COVID-19 - virology</subject><subject>COVID-19 Nucleic Acid Testing</subject><subject>Disease transmission</subject><subject>Family Medicine</subject><subject>Female</subject><subject>General Practice</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Infectious Diseases</subject><subject>Internal Medicine</subject><subject>Laboratories</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Middle Aged</subject><subject>Nosocomial infection</subject><subject>Original Paper</subject><subject>Polymerase chain reaction</subject><subject>Reverse transcription</subject><subject>RNA, Viral - genetics</subject><subject>RNA, Viral - isolation & purification</subject><subject>SARS-CoV-2</subject><subject>SARS-CoV-2 - genetics</subject><subject>SARS-CoV-2 - growth & development</subject><subject>SARS-CoV-2 - isolation & purification</subject><subject>Secretions</subject><subject>Severe acute respiratory syndrome coronavirus 2</subject><subject>Signs and symptoms</subject><subject>Vero Cells</subject><subject>Viral diseases</subject><subject>Virus Shedding</subject><subject>Young Adult</subject><issn>0300-8126</issn><issn>1439-0973</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kU9rFTEUxYMo9ln9Ai4k4MZN6k1yM5PZCOVZrVAoWBV3IZNm2nnMJM8k09Jvb-qr9c-ii3AX53dPzuUQ8pLDAQdo32augAMDUR9XApl4RFYcZcega-VjsgIJwDQXzR55lvMGAFSH7VOyJ0WHqBFW5Pt7X7wrYww0DvTs8PMZW8dvTNAxUBfDZglVvLITzd4lf8tlOqQ4060tow8l0-uxXMal0OiWySaab-ZtiXN-Tp4Mdsr-xd3cJ18_HH1ZH7OT04-f1ocnzGGLhQ2AvoWu50oN57zBQbdiaBx4axsvtVMaVaMbrMn73jrbc-dB8d4Jx60DkPvk3c53u_SzP3c1U7KT2aZxtunGRDuaf5UwXpqLeGVa7Fqtmmrw5s4gxR-Lz8XMY3Z-mmzwcclGIKLUQnZY0df_oZu4pFDPM0IBNpJL3VVK7CiXYs7JD_dhOJjb4syuOFOLM7-KM6Iuvfr7jPuV301VQO6AXKVw4dOfvx-w_Qksh6SG</recordid><startdate>20210401</startdate><enddate>20210401</enddate><creator>Li, Xin</creator><creator>Chan, Jasper Fuk-Woo</creator><creator>Li, Kenneth Kai-Wang</creator><creator>Tso, Eugene Yuk-Keung</creator><creator>Yip, Cyril Chik-Yan</creator><creator>Sridhar, Siddharth</creator><creator>Chung, Tom Wai-Hin</creator><creator>Chiu, Kelvin Hei-Yeung</creator><creator>Hung, Derek Ling-Lung</creator><creator>Wu, Alan Ka-Lun</creator><creator>Chau, Sandy Ka-Yee</creator><creator>Liu, Raymond</creator><creator>Lung, Kwok-Cheung</creator><creator>Tam, Anthony Raymond</creator><creator>Cheng, Vincent Chi-Chung</creator><creator>To, Kelvin Kai-Wang</creator><creator>Chan, Kwok-Hung</creator><creator>Hung, Ivan Fan-Ngai</creator><creator>Yuen, Kwok-Yung</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7RV</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20210401</creationdate><title>Detection of SARS-CoV-2 in conjunctival secretions from patients without ocular symptoms</title><author>Li, Xin ; Chan, Jasper Fuk-Woo ; Li, Kenneth Kai-Wang ; Tso, Eugene Yuk-Keung ; Yip, Cyril Chik-Yan ; Sridhar, Siddharth ; Chung, Tom Wai-Hin ; Chiu, Kelvin Hei-Yeung ; Hung, Derek Ling-Lung ; Wu, Alan Ka-Lun ; Chau, Sandy Ka-Yee ; Liu, Raymond ; Lung, Kwok-Cheung ; Tam, Anthony Raymond ; Cheng, Vincent Chi-Chung ; To, Kelvin Kai-Wang ; Chan, Kwok-Hung ; Hung, Ivan Fan-Ngai ; Yuen, Kwok-Yung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-f04e709b155fd164f872f6c0eaa6e38c58456864059bbacab1ce051bc2c1ac003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Animals</topic><topic>Chlorocebus aethiops</topic><topic>Conjunctiva - virology</topic><topic>Coronaviruses</topic><topic>COVID-19</topic><topic>COVID-19 - diagnosis</topic><topic>COVID-19 - virology</topic><topic>COVID-19 Nucleic Acid Testing</topic><topic>Disease transmission</topic><topic>Family Medicine</topic><topic>Female</topic><topic>General Practice</topic><topic>Hospitals</topic><topic>Humans</topic><topic>Infectious Diseases</topic><topic>Internal Medicine</topic><topic>Laboratories</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Middle Aged</topic><topic>Nosocomial infection</topic><topic>Original Paper</topic><topic>Polymerase chain reaction</topic><topic>Reverse transcription</topic><topic>RNA, Viral - genetics</topic><topic>RNA, Viral - isolation & purification</topic><topic>SARS-CoV-2</topic><topic>SARS-CoV-2 - genetics</topic><topic>SARS-CoV-2 - growth & development</topic><topic>SARS-CoV-2 - isolation & purification</topic><topic>Secretions</topic><topic>Severe acute respiratory syndrome coronavirus 2</topic><topic>Signs and symptoms</topic><topic>Vero Cells</topic><topic>Viral diseases</topic><topic>Virus Shedding</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Xin</creatorcontrib><creatorcontrib>Chan, Jasper Fuk-Woo</creatorcontrib><creatorcontrib>Li, Kenneth Kai-Wang</creatorcontrib><creatorcontrib>Tso, Eugene Yuk-Keung</creatorcontrib><creatorcontrib>Yip, Cyril Chik-Yan</creatorcontrib><creatorcontrib>Sridhar, Siddharth</creatorcontrib><creatorcontrib>Chung, Tom Wai-Hin</creatorcontrib><creatorcontrib>Chiu, Kelvin Hei-Yeung</creatorcontrib><creatorcontrib>Hung, Derek Ling-Lung</creatorcontrib><creatorcontrib>Wu, Alan Ka-Lun</creatorcontrib><creatorcontrib>Chau, Sandy Ka-Yee</creatorcontrib><creatorcontrib>Liu, Raymond</creatorcontrib><creatorcontrib>Lung, Kwok-Cheung</creatorcontrib><creatorcontrib>Tam, Anthony Raymond</creatorcontrib><creatorcontrib>Cheng, Vincent Chi-Chung</creatorcontrib><creatorcontrib>To, Kelvin Kai-Wang</creatorcontrib><creatorcontrib>Chan, Kwok-Hung</creatorcontrib><creatorcontrib>Hung, Ivan Fan-Ngai</creatorcontrib><creatorcontrib>Yuen, Kwok-Yung</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nursing & Allied Health Database</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Infection</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Xin</au><au>Chan, Jasper Fuk-Woo</au><au>Li, Kenneth Kai-Wang</au><au>Tso, Eugene Yuk-Keung</au><au>Yip, Cyril Chik-Yan</au><au>Sridhar, Siddharth</au><au>Chung, Tom Wai-Hin</au><au>Chiu, Kelvin Hei-Yeung</au><au>Hung, Derek Ling-Lung</au><au>Wu, Alan Ka-Lun</au><au>Chau, Sandy Ka-Yee</au><au>Liu, Raymond</au><au>Lung, Kwok-Cheung</au><au>Tam, Anthony Raymond</au><au>Cheng, Vincent Chi-Chung</au><au>To, Kelvin Kai-Wang</au><au>Chan, Kwok-Hung</au><au>Hung, Ivan Fan-Ngai</au><au>Yuen, Kwok-Yung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of SARS-CoV-2 in conjunctival secretions from patients without ocular symptoms</atitle><jtitle>Infection</jtitle><stitle>Infection</stitle><addtitle>Infection</addtitle><date>2021-04-01</date><risdate>2021</risdate><volume>49</volume><issue>2</issue><spage>257</spage><epage>265</epage><pages>257-265</pages><issn>0300-8126</issn><eissn>1439-0973</eissn><abstract>Purpose
To evaluate the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in conjunctival secretions from patients without ocular symptoms.
Methods
Conjunctival swabs were prospectively collected from laboratory-confirmed Coronavirus disease 2019 (COVID-19) patients without ocular symptoms for reverse transcription-polymerase chain reaction (RT-PCR) and viral culture.
Results
A total of 158 conjunctival swabs were obtained from 49 laboratory-confirmed COVID-19 patients. The median duration of illness when the first conjunctival swab was obtained was 10 days (range 2–27 days). Four conjunctival swabs from four different patients (4/49, 8.2%) were positive for SARS-CoV-2 RNA by RT-PCR. The Ct values ranged from 32.7 to 37.7 (mean 35.4). Viral cultures were negative for all four RT-PCR-positive conjunctival swabs.
Conclusion
Conjunctival secretions of a minority of COVID-19 patients without ocular symptoms may contain relatively low levels of SARS-CoV-2 RNA, but their infectiousness remains undetermined. Appropriate infection control measures should be implemented during ophthalmological assessment of COVID-19 patients to prevent potential nosocomial transmission of SARS-CoV-2.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>32944840</pmid><doi>10.1007/s15010-020-01524-2</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Infection, 2021-04, Vol.49 (2), p.257-265 |
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| subjects | Adult Aged Aged, 80 and over Animals Chlorocebus aethiops Conjunctiva - virology Coronaviruses COVID-19 COVID-19 - diagnosis COVID-19 - virology COVID-19 Nucleic Acid Testing Disease transmission Family Medicine Female General Practice Hospitals Humans Infectious Diseases Internal Medicine Laboratories Male Medicine Medicine & Public Health Middle Aged Nosocomial infection Original Paper Polymerase chain reaction Reverse transcription RNA, Viral - genetics RNA, Viral - isolation & purification SARS-CoV-2 SARS-CoV-2 - genetics SARS-CoV-2 - growth & development SARS-CoV-2 - isolation & purification Secretions Severe acute respiratory syndrome coronavirus 2 Signs and symptoms Vero Cells Viral diseases Virus Shedding Young Adult |
| title | Detection of SARS-CoV-2 in conjunctival secretions from patients without ocular symptoms |
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