Exosomes derived from chemically induced human hepatic progenitors inhibit oxidative stress induced cell death
The emerging field of regenerative medicine has revealed that the exosome contributes to many aspects of development and disease through intercellular communication between donor and recipient cells. However, the biological functions of exosomes secreted from cells have remained largely unexplored....
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| Veröffentlicht in: | Biotechnology and bioengineering 2020-09, Vol.117 (9), p.2658-2667 |
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| container_title | Biotechnology and bioengineering |
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| creator | Hyung, Sujin Jeong, Jaemin Shin, Kyusoon Kim, Ju Young Yim, Ji‐Hye Yu, Chan Jong Jung, Hyun Suk Hwang, Kyung‐Gyun Choi, Dongho Hong, Jong Wook |
| description | The emerging field of regenerative medicine has revealed that the exosome contributes to many aspects of development and disease through intercellular communication between donor and recipient cells. However, the biological functions of exosomes secreted from cells have remained largely unexplored. Here, we report that the human hepatic progenitor cells (CdHs)‐derived exosome (EXOhCdHs) plays a crucial role in maintaining cell viability. The inhibition of exosome secretion treatment with GW4869 results in the acceleration of reactive oxygen species (ROS) production, thereby causing a decrease of cell viability. This event provokes inhibition of caspase dependent cell death signaling, leading to a ROS‐dependent cell damage response and thus induces promotion of antioxidant gene expression or repair of cell death of hypoxia‐exposed cells. Together, these findings show the effect of exosomes in regeneration of liver cells, and offer valuable new insights into liver regeneration.
Protective effect of hCdHs‐derived exosome against oxidative stress in hepatocyte. Exosomes were isolated from chemically induced human hepatic progenitors (hCdHs) by using ‘H’ method. hCdHs‐derived exosome (EXOhCdHs) has a beneficial effect on antioxidant activity of damaged hepatocytes. |
| doi_str_mv | 10.1002/bit.27447 |
| format | Article |
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Protective effect of hCdHs‐derived exosome against oxidative stress in hepatocyte. Exosomes were isolated from chemically induced human hepatic progenitors (hCdHs) by using ‘H’ method. hCdHs‐derived exosome (EXOhCdHs) has a beneficial effect on antioxidant activity of damaged hepatocytes.</description><identifier>ISSN: 0006-3592</identifier><identifier>EISSN: 1097-0290</identifier><identifier>DOI: 10.1002/bit.27447</identifier><identifier>PMID: 32484909</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc</publisher><subject>antioxidant activity ; Antioxidants ; Apoptosis ; Caspase ; Cell death ; Cell interactions ; Cell signaling ; cell survival ; Cell viability ; exosome therapy ; Exosomes ; Gene expression ; Hepatocytes ; human hepatic progenitor cells‐derived exosome (EXOhCdHs) ; Hypoxia ; Liver ; liver disease ; Mortality ; Oxidative stress ; Progenitor cells ; Reactive oxygen species ; Regeneration (physiology) ; Regenerative medicine ; Stem cells</subject><ispartof>Biotechnology and bioengineering, 2020-09, Vol.117 (9), p.2658-2667</ispartof><rights>2020 The Authors. Published by Wiley Periodicals LLC</rights><rights>2020. This article is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5237-3d0430508910307a82f8ca3108dc2ca342d19cda53c407c7a4200255eb7611693</citedby><cites>FETCH-LOGICAL-c5237-3d0430508910307a82f8ca3108dc2ca342d19cda53c407c7a4200255eb7611693</cites><orcidid>0000-0003-1192-0972 ; 0000-0001-9529-5400</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbit.27447$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbit.27447$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,1411,27901,27902,45550,45551</link.rule.ids></links><search><creatorcontrib>Hyung, Sujin</creatorcontrib><creatorcontrib>Jeong, Jaemin</creatorcontrib><creatorcontrib>Shin, Kyusoon</creatorcontrib><creatorcontrib>Kim, Ju Young</creatorcontrib><creatorcontrib>Yim, Ji‐Hye</creatorcontrib><creatorcontrib>Yu, Chan Jong</creatorcontrib><creatorcontrib>Jung, Hyun Suk</creatorcontrib><creatorcontrib>Hwang, Kyung‐Gyun</creatorcontrib><creatorcontrib>Choi, Dongho</creatorcontrib><creatorcontrib>Hong, Jong Wook</creatorcontrib><title>Exosomes derived from chemically induced human hepatic progenitors inhibit oxidative stress induced cell death</title><title>Biotechnology and bioengineering</title><description>The emerging field of regenerative medicine has revealed that the exosome contributes to many aspects of development and disease through intercellular communication between donor and recipient cells. However, the biological functions of exosomes secreted from cells have remained largely unexplored. Here, we report that the human hepatic progenitor cells (CdHs)‐derived exosome (EXOhCdHs) plays a crucial role in maintaining cell viability. The inhibition of exosome secretion treatment with GW4869 results in the acceleration of reactive oxygen species (ROS) production, thereby causing a decrease of cell viability. This event provokes inhibition of caspase dependent cell death signaling, leading to a ROS‐dependent cell damage response and thus induces promotion of antioxidant gene expression or repair of cell death of hypoxia‐exposed cells. Together, these findings show the effect of exosomes in regeneration of liver cells, and offer valuable new insights into liver regeneration.
Protective effect of hCdHs‐derived exosome against oxidative stress in hepatocyte. Exosomes were isolated from chemically induced human hepatic progenitors (hCdHs) by using ‘H’ method. hCdHs‐derived exosome (EXOhCdHs) has a beneficial effect on antioxidant activity of damaged hepatocytes.</description><subject>antioxidant activity</subject><subject>Antioxidants</subject><subject>Apoptosis</subject><subject>Caspase</subject><subject>Cell death</subject><subject>Cell interactions</subject><subject>Cell signaling</subject><subject>cell survival</subject><subject>Cell viability</subject><subject>exosome therapy</subject><subject>Exosomes</subject><subject>Gene expression</subject><subject>Hepatocytes</subject><subject>human hepatic progenitor cells‐derived exosome (EXOhCdHs)</subject><subject>Hypoxia</subject><subject>Liver</subject><subject>liver disease</subject><subject>Mortality</subject><subject>Oxidative stress</subject><subject>Progenitor cells</subject><subject>Reactive oxygen species</subject><subject>Regeneration (physiology)</subject><subject>Regenerative medicine</subject><subject>Stem cells</subject><issn>0006-3592</issn><issn>1097-0290</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><recordid>eNp1kUtPGzEQgK2qqAnQQ__BSr2Uw8L4sev1pVKLKEVC4gJny7EnWaPddWrvpuTf4zRRpCJx8mO--TQPQr5QuKQA7Grhx0smhZAfyJyCkiUwBR_JHADqkleKzchpSs_5KZu6_kRmnIlGKFBzMty8hBR6TIXD6DfoimUMfWFb7L01Xbct_OAmm__bqTdD0eLajN4W6xhWOPgxxJSJ1ucSivDiXQ5usEhjxJSOqRa7LvvN2J6Tk6XpEn4-nGfk6dfN4_Xv8v7h9u76x31pK8ZlyR0IDhU0igIHaRq2bKzhFBpnWb4I5qiyzlTcCpBWGsHyHKoKF7KmtFb8jHzfe9fTokdncRij6fQ6-t7ErQ7G6_8jg2_1Kmy0FKquBc-CbwdBDH8mTKPufdr1YQYMU9JMgKKKKcEy-vUN-hymOOT2MsU54w3ATnixp2wMKUVcHouhoHdb1HmE-t8WM3u1Z__6Drfvg_rn3eM-4xWIQ54D</recordid><startdate>202009</startdate><enddate>202009</enddate><creator>Hyung, Sujin</creator><creator>Jeong, Jaemin</creator><creator>Shin, Kyusoon</creator><creator>Kim, Ju Young</creator><creator>Yim, Ji‐Hye</creator><creator>Yu, Chan Jong</creator><creator>Jung, Hyun Suk</creator><creator>Hwang, Kyung‐Gyun</creator><creator>Choi, Dongho</creator><creator>Hong, Jong Wook</creator><general>Wiley Subscription Services, Inc</general><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-1192-0972</orcidid><orcidid>https://orcid.org/0000-0001-9529-5400</orcidid></search><sort><creationdate>202009</creationdate><title>Exosomes derived from chemically induced human hepatic progenitors inhibit oxidative stress induced cell death</title><author>Hyung, Sujin ; 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However, the biological functions of exosomes secreted from cells have remained largely unexplored. Here, we report that the human hepatic progenitor cells (CdHs)‐derived exosome (EXOhCdHs) plays a crucial role in maintaining cell viability. The inhibition of exosome secretion treatment with GW4869 results in the acceleration of reactive oxygen species (ROS) production, thereby causing a decrease of cell viability. This event provokes inhibition of caspase dependent cell death signaling, leading to a ROS‐dependent cell damage response and thus induces promotion of antioxidant gene expression or repair of cell death of hypoxia‐exposed cells. Together, these findings show the effect of exosomes in regeneration of liver cells, and offer valuable new insights into liver regeneration.
Protective effect of hCdHs‐derived exosome against oxidative stress in hepatocyte. Exosomes were isolated from chemically induced human hepatic progenitors (hCdHs) by using ‘H’ method. hCdHs‐derived exosome (EXOhCdHs) has a beneficial effect on antioxidant activity of damaged hepatocytes.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc</pub><pmid>32484909</pmid><doi>10.1002/bit.27447</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-1192-0972</orcidid><orcidid>https://orcid.org/0000-0001-9529-5400</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | antioxidant activity Antioxidants Apoptosis Caspase Cell death Cell interactions Cell signaling cell survival Cell viability exosome therapy Exosomes Gene expression Hepatocytes human hepatic progenitor cells‐derived exosome (EXOhCdHs) Hypoxia Liver liver disease Mortality Oxidative stress Progenitor cells Reactive oxygen species Regeneration (physiology) Regenerative medicine Stem cells |
| title | Exosomes derived from chemically induced human hepatic progenitors inhibit oxidative stress induced cell death |
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