Outbreak of botulism type A in dairy cows detected by MALDI-TOF mass spectrometry
Twenty-eight lactating dairy cattle in New York State were exposed to botulism toxin; 12 died and 16 recovered but never returned to full productivity. Pieces of a raccoon carcass were found in the total mixed ration on the first day of the outbreak. Clinical signs included anorexia, decreased milk...
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| Veröffentlicht in: | Journal of veterinary diagnostic investigation 2020-09, Vol.32 (5), p.722-726 |
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| creator | Frye, Elisha A. Egan, Christina Perry, Michael J. Crouch, Esther E. Burbank, Kyle E. Kelly, Kathleen M. |
| description | Twenty-eight lactating dairy cattle in New York State were exposed to botulism toxin; 12 died and 16 recovered but never returned to full productivity. Pieces of a raccoon carcass were found in the total mixed ration on the first day of the outbreak. Clinical signs included anorexia, decreased milk production, decreased tongue tone, profound weakness, and recumbency. Clostridium botulinum type A (BoNT/A) was detected in rumen contents from 2 deceased cows via matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). In addition, C. botulinum type C was cultured from the liver of a third cow, and C. botulinum neurotoxin-producing type C gene (bont/C) was detected via real-time PCR. On postmortem examination, 4 cows had findings suggestive of toxic myopathy, but the cause and significance of these lesions is unknown given that botulism is typically not associated with gross or histologic lesions. This outbreak of BoNT/A in cattle in North America was diagnosed via MALDI-TOF MS, a rapid and sensitive modality for detection of botulinum preformed neurotoxin. |
| doi_str_mv | 10.1177/1040638720943127 |
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Pieces of a raccoon carcass were found in the total mixed ration on the first day of the outbreak. Clinical signs included anorexia, decreased milk production, decreased tongue tone, profound weakness, and recumbency. Clostridium botulinum type A (BoNT/A) was detected in rumen contents from 2 deceased cows via matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). In addition, C. botulinum type C was cultured from the liver of a third cow, and C. botulinum neurotoxin-producing type C gene (bont/C) was detected via real-time PCR. On postmortem examination, 4 cows had findings suggestive of toxic myopathy, but the cause and significance of these lesions is unknown given that botulism is typically not associated with gross or histologic lesions. This outbreak of BoNT/A in cattle in North America was diagnosed via MALDI-TOF MS, a rapid and sensitive modality for detection of botulinum preformed neurotoxin.</description><identifier>ISSN: 1040-6387</identifier><identifier>EISSN: 1943-4936</identifier><identifier>DOI: 10.1177/1040638720943127</identifier><identifier>PMID: 32715936</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>Animals ; Botulinum Toxins - analysis ; Botulism - diagnosis ; Botulism - epidemiology ; Botulism - veterinary ; Brief Communication ; Cattle ; Cattle Diseases - diagnosis ; Cattle Diseases - epidemiology ; Clostridium botulinum - isolation & purification ; Disease Outbreaks - veterinary ; Female ; New York - epidemiology ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - veterinary</subject><ispartof>Journal of veterinary diagnostic investigation, 2020-09, Vol.32 (5), p.722-726</ispartof><rights>2020 The Author(s)</rights><rights>2020 The Author(s) 2020 American Association of Veterinary Laboratory Diagnosticians</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c434t-ab863a1306ee938568fd1025bf5773867742275cd17e81d44fe95257fcd797383</citedby><cites>FETCH-LOGICAL-c434t-ab863a1306ee938568fd1025bf5773867742275cd17e81d44fe95257fcd797383</cites><orcidid>0000-0002-7784-6771</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7488966/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7488966/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,21798,27901,27902,43597,43598,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32715936$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Frye, Elisha A.</creatorcontrib><creatorcontrib>Egan, Christina</creatorcontrib><creatorcontrib>Perry, Michael J.</creatorcontrib><creatorcontrib>Crouch, Esther E.</creatorcontrib><creatorcontrib>Burbank, Kyle E.</creatorcontrib><creatorcontrib>Kelly, Kathleen M.</creatorcontrib><title>Outbreak of botulism type A in dairy cows detected by MALDI-TOF mass spectrometry</title><title>Journal of veterinary diagnostic investigation</title><addtitle>J Vet Diagn Invest</addtitle><description>Twenty-eight lactating dairy cattle in New York State were exposed to botulism toxin; 12 died and 16 recovered but never returned to full productivity. Pieces of a raccoon carcass were found in the total mixed ration on the first day of the outbreak. Clinical signs included anorexia, decreased milk production, decreased tongue tone, profound weakness, and recumbency. Clostridium botulinum type A (BoNT/A) was detected in rumen contents from 2 deceased cows via matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). In addition, C. botulinum type C was cultured from the liver of a third cow, and C. botulinum neurotoxin-producing type C gene (bont/C) was detected via real-time PCR. On postmortem examination, 4 cows had findings suggestive of toxic myopathy, but the cause and significance of these lesions is unknown given that botulism is typically not associated with gross or histologic lesions. This outbreak of BoNT/A in cattle in North America was diagnosed via MALDI-TOF MS, a rapid and sensitive modality for detection of botulinum preformed neurotoxin.</description><subject>Animals</subject><subject>Botulinum Toxins - analysis</subject><subject>Botulism - diagnosis</subject><subject>Botulism - epidemiology</subject><subject>Botulism - veterinary</subject><subject>Brief Communication</subject><subject>Cattle</subject><subject>Cattle Diseases - diagnosis</subject><subject>Cattle Diseases - epidemiology</subject><subject>Clostridium botulinum - isolation & purification</subject><subject>Disease Outbreaks - veterinary</subject><subject>Female</subject><subject>New York - epidemiology</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - veterinary</subject><issn>1040-6387</issn><issn>1943-4936</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kFFLwzAUhYMobk7ffZL8gWrSpEn6IozpdDAZwnwOaZPOzrUpSab035sxHSr4dC-c75x7OQBcYnSNMec3GFHEiOApyinBKT8CQxy3hOaEHcc9yslOH4Az79cIZWnG8SkYkJTjLDJD8LzYhsIZ9QZtBQsbtpvaNzD0nYFjWLdQq9r1sLQfHmoTTBmMhkUPn8bzu1myXExho7yHvouKs40Jrj8HJ5XaeHPxNUfgZXq_nDwm88XDbDKeJyUlNCSqEIwoTBAzJiciY6LSGKVZUWWcE8E4p2nKs1JjbgTWlFYm371flZrnESAjcLvP7bZFY3Rp2uDURnaubpTrpVW1_K209atc2XfJqRA5YzEA7QNKZ713pjp4MZK7euXfeqPl6ufNg-G7zwgke8CrlZFru3Vt7OD_wE8Fq4HU</recordid><startdate>20200901</startdate><enddate>20200901</enddate><creator>Frye, Elisha A.</creator><creator>Egan, Christina</creator><creator>Perry, Michael J.</creator><creator>Crouch, Esther E.</creator><creator>Burbank, Kyle E.</creator><creator>Kelly, Kathleen M.</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-7784-6771</orcidid></search><sort><creationdate>20200901</creationdate><title>Outbreak of botulism type A in dairy cows detected by MALDI-TOF mass spectrometry</title><author>Frye, Elisha A. ; Egan, Christina ; Perry, Michael J. ; Crouch, Esther E. ; Burbank, Kyle E. ; Kelly, Kathleen M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c434t-ab863a1306ee938568fd1025bf5773867742275cd17e81d44fe95257fcd797383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Botulinum Toxins - analysis</topic><topic>Botulism - diagnosis</topic><topic>Botulism - epidemiology</topic><topic>Botulism - veterinary</topic><topic>Brief Communication</topic><topic>Cattle</topic><topic>Cattle Diseases - diagnosis</topic><topic>Cattle Diseases - epidemiology</topic><topic>Clostridium botulinum - isolation & purification</topic><topic>Disease Outbreaks - veterinary</topic><topic>Female</topic><topic>New York - epidemiology</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - veterinary</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Frye, Elisha A.</creatorcontrib><creatorcontrib>Egan, Christina</creatorcontrib><creatorcontrib>Perry, Michael J.</creatorcontrib><creatorcontrib>Crouch, Esther E.</creatorcontrib><creatorcontrib>Burbank, Kyle E.</creatorcontrib><creatorcontrib>Kelly, Kathleen M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of veterinary diagnostic investigation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Frye, Elisha A.</au><au>Egan, Christina</au><au>Perry, Michael J.</au><au>Crouch, Esther E.</au><au>Burbank, Kyle E.</au><au>Kelly, Kathleen M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Outbreak of botulism type A in dairy cows detected by MALDI-TOF mass spectrometry</atitle><jtitle>Journal of veterinary diagnostic investigation</jtitle><addtitle>J Vet Diagn Invest</addtitle><date>2020-09-01</date><risdate>2020</risdate><volume>32</volume><issue>5</issue><spage>722</spage><epage>726</epage><pages>722-726</pages><issn>1040-6387</issn><eissn>1943-4936</eissn><abstract>Twenty-eight lactating dairy cattle in New York State were exposed to botulism toxin; 12 died and 16 recovered but never returned to full productivity. Pieces of a raccoon carcass were found in the total mixed ration on the first day of the outbreak. Clinical signs included anorexia, decreased milk production, decreased tongue tone, profound weakness, and recumbency. Clostridium botulinum type A (BoNT/A) was detected in rumen contents from 2 deceased cows via matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). In addition, C. botulinum type C was cultured from the liver of a third cow, and C. botulinum neurotoxin-producing type C gene (bont/C) was detected via real-time PCR. On postmortem examination, 4 cows had findings suggestive of toxic myopathy, but the cause and significance of these lesions is unknown given that botulism is typically not associated with gross or histologic lesions. This outbreak of BoNT/A in cattle in North America was diagnosed via MALDI-TOF MS, a rapid and sensitive modality for detection of botulinum preformed neurotoxin.</abstract><cop>Los Angeles, CA</cop><pub>SAGE Publications</pub><pmid>32715936</pmid><doi>10.1177/1040638720943127</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0002-7784-6771</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Botulinum Toxins - analysis Botulism - diagnosis Botulism - epidemiology Botulism - veterinary Brief Communication Cattle Cattle Diseases - diagnosis Cattle Diseases - epidemiology Clostridium botulinum - isolation & purification Disease Outbreaks - veterinary Female New York - epidemiology Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - veterinary |
| title | Outbreak of botulism type A in dairy cows detected by MALDI-TOF mass spectrometry |
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