Electroacupuncture Alleviates Osteoarthritis by Suppressing NLRP3 Inflammasome Activation in Guinea Pigs
Osteoarthritis (OA) is an increasingly prevalent disease affecting synovial joints, which includes joint degeneration, inflammation, and joint pain. The activation of nucleotide-binding and oligomerization domain-like receptor containing protein 3 (NLRP3) could promote synovial inflammation. Previou...
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| Veröffentlicht in: | Evidence-based complementary and alternative medicine 2020, Vol.2020 (2020), p.1-8 |
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| creator | Yuan, Puwei Li, Xiaoqun Yu, Hongchao Kang, Wulin Wang, Bo Chen, Mo Wang, Zhankui Dong, Bo |
| description | Osteoarthritis (OA) is an increasingly prevalent disease affecting synovial joints, which includes joint degeneration, inflammation, and joint pain. The activation of nucleotide-binding and oligomerization domain-like receptor containing protein 3 (NLRP3) could promote synovial inflammation. Previous studies have shown that electroacupuncture (EA) has potential anti-inflammatory effect. However, the effect of EA treatment on OA remains unclear. The aim of this study was to investigate the effect of applied EA on OA and joint pain and its relationship with NLRP3 inflammasome. The Hartley guinea pigs with naturally occurring OA at age 18 months were chosen as the OA model and treated with EA for 4 weeks. Mechanical allodynia was quantified by using von Frey filaments. The expression of NLRP3 inflammasome and the downstream proinflammatory factors in the cartilage tissue were quantified. Our results showed that EA treatment significantly reduces mechanical allodynia, improves the articular cartilage structure, and decreases the fibrillation on the cartilage surface in guinea pigs with spontaneous osteoarthritis. Moreover, we also found that EA treatment attenuates the NLRP3 inflammasome activation and suppresses the protein expression levels of caspase-1 and IL-1β in the cartilage tissue. Our findings suggest that EA treatment attenuates OA and joint pain by suppressing NLRP3 inflammasome activation and support further investigation of the potential therapeutic tactics. |
| doi_str_mv | 10.1155/2020/5476064 |
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The activation of nucleotide-binding and oligomerization domain-like receptor containing protein 3 (NLRP3) could promote synovial inflammation. Previous studies have shown that electroacupuncture (EA) has potential anti-inflammatory effect. However, the effect of EA treatment on OA remains unclear. The aim of this study was to investigate the effect of applied EA on OA and joint pain and its relationship with NLRP3 inflammasome. The Hartley guinea pigs with naturally occurring OA at age 18 months were chosen as the OA model and treated with EA for 4 weeks. Mechanical allodynia was quantified by using von Frey filaments. The expression of NLRP3 inflammasome and the downstream proinflammatory factors in the cartilage tissue were quantified. Our results showed that EA treatment significantly reduces mechanical allodynia, improves the articular cartilage structure, and decreases the fibrillation on the cartilage surface in guinea pigs with spontaneous osteoarthritis. Moreover, we also found that EA treatment attenuates the NLRP3 inflammasome activation and suppresses the protein expression levels of caspase-1 and IL-1β in the cartilage tissue. Our findings suggest that EA treatment attenuates OA and joint pain by suppressing NLRP3 inflammasome activation and support further investigation of the potential therapeutic tactics.</description><identifier>ISSN: 1741-427X</identifier><identifier>EISSN: 1741-4288</identifier><identifier>DOI: 10.1155/2020/5476064</identifier><identifier>PMID: 32952587</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Acupuncture ; Arthritis ; Cartilage ; Cartilage (articular) ; Cartilage diseases ; Caspase-1 ; Degeneration ; Electroacupuncture ; Fibrillation ; Filaments ; IL-1β ; Inflammasomes ; Inflammation ; Joint diseases ; Oligomerization ; Osteoarthritis ; Pain ; Pain perception ; Protein expression ; Proteins ; Software ; Tumor necrosis factor-TNF</subject><ispartof>Evidence-based complementary and alternative medicine, 2020, Vol.2020 (2020), p.1-8</ispartof><rights>Copyright © 2020 Zhankui Wang et al.</rights><rights>Copyright © 2020 Zhankui Wang et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2020 Zhankui Wang et al. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c448t-c3b33998b823eb455f8cf42eb2a7de66f6a21b97c51b49eca66d8689f5432c1a3</citedby><cites>FETCH-LOGICAL-c448t-c3b33998b823eb455f8cf42eb2a7de66f6a21b97c51b49eca66d8689f5432c1a3</cites><orcidid>0000-0001-7916-8823 ; 0000-0003-4715-0973</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7487102/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7487102/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,4022,27921,27922,27923,53789,53791</link.rule.ids></links><search><contributor>Ren, Ke</contributor><contributor>Ke Ren</contributor><creatorcontrib>Yuan, Puwei</creatorcontrib><creatorcontrib>Li, Xiaoqun</creatorcontrib><creatorcontrib>Yu, Hongchao</creatorcontrib><creatorcontrib>Kang, Wulin</creatorcontrib><creatorcontrib>Wang, Bo</creatorcontrib><creatorcontrib>Chen, Mo</creatorcontrib><creatorcontrib>Wang, Zhankui</creatorcontrib><creatorcontrib>Dong, Bo</creatorcontrib><title>Electroacupuncture Alleviates Osteoarthritis by Suppressing NLRP3 Inflammasome Activation in Guinea Pigs</title><title>Evidence-based complementary and alternative medicine</title><description>Osteoarthritis (OA) is an increasingly prevalent disease affecting synovial joints, which includes joint degeneration, inflammation, and joint pain. The activation of nucleotide-binding and oligomerization domain-like receptor containing protein 3 (NLRP3) could promote synovial inflammation. Previous studies have shown that electroacupuncture (EA) has potential anti-inflammatory effect. However, the effect of EA treatment on OA remains unclear. The aim of this study was to investigate the effect of applied EA on OA and joint pain and its relationship with NLRP3 inflammasome. The Hartley guinea pigs with naturally occurring OA at age 18 months were chosen as the OA model and treated with EA for 4 weeks. Mechanical allodynia was quantified by using von Frey filaments. The expression of NLRP3 inflammasome and the downstream proinflammatory factors in the cartilage tissue were quantified. Our results showed that EA treatment significantly reduces mechanical allodynia, improves the articular cartilage structure, and decreases the fibrillation on the cartilage surface in guinea pigs with spontaneous osteoarthritis. Moreover, we also found that EA treatment attenuates the NLRP3 inflammasome activation and suppresses the protein expression levels of caspase-1 and IL-1β in the cartilage tissue. Our findings suggest that EA treatment attenuates OA and joint pain by suppressing NLRP3 inflammasome activation and support further investigation of the potential therapeutic tactics.</description><subject>Acupuncture</subject><subject>Arthritis</subject><subject>Cartilage</subject><subject>Cartilage (articular)</subject><subject>Cartilage diseases</subject><subject>Caspase-1</subject><subject>Degeneration</subject><subject>Electroacupuncture</subject><subject>Fibrillation</subject><subject>Filaments</subject><subject>IL-1β</subject><subject>Inflammasomes</subject><subject>Inflammation</subject><subject>Joint diseases</subject><subject>Oligomerization</subject><subject>Osteoarthritis</subject><subject>Pain</subject><subject>Pain perception</subject><subject>Protein expression</subject><subject>Proteins</subject><subject>Software</subject><subject>Tumor necrosis factor-TNF</subject><issn>1741-427X</issn><issn>1741-4288</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqF0c1rFDEYBvBBFFurN88S8CLo2kkmnxehlLYWFlv8AG_hnew7uykzyZhkVvrfd5ZdKnrxkgTyy8Mbnqp6TeuPlApxympWnwquZC35k-qYKk4XnGn99PGsfh5VL3K-q2tmlFLPq6OGGcGEVsfV5qJHV1IEN41TcGVKSM76HrceCmZykwtGSGWTfPGZtPfk2zSOCXP2YU2-LL_eNuQ6dD0MA-Q4zG9d8VsoPgbiA7mafEAgt36dX1bPOugzvjrsJ9WPy4vv558Xy5ur6_Oz5cJxrsvCNW3TGKNbzRpsuRCddh1n2DJQK5Syk8Boa5QTtOUGHUi50lKbTvCGOQrNSfVpnztO7YArh6Ek6O2Y_ADp3kbw9u-b4Dd2HbdWca1ozeaAd4eAFH9NmIsdfHbY9xAwTtkyzrmsxbzO9O0_9C5OKczf2ylGuVHczOrDXrkUc07YPQ5Da7ur0O4qtIcKZ_5-zzc-rOC3_59-s9c4G-zgj6ZCCq2bB8p9pZE</recordid><startdate>2020</startdate><enddate>2020</enddate><creator>Yuan, Puwei</creator><creator>Li, Xiaoqun</creator><creator>Yu, Hongchao</creator><creator>Kang, Wulin</creator><creator>Wang, Bo</creator><creator>Chen, Mo</creator><creator>Wang, Zhankui</creator><creator>Dong, Bo</creator><general>Hindawi Publishing Corporation</general><general>Hindawi</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7T5</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88G</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M2M</scope><scope>M2O</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-7916-8823</orcidid><orcidid>https://orcid.org/0000-0003-4715-0973</orcidid></search><sort><creationdate>2020</creationdate><title>Electroacupuncture Alleviates Osteoarthritis by Suppressing NLRP3 Inflammasome Activation in Guinea Pigs</title><author>Yuan, Puwei ; 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The activation of nucleotide-binding and oligomerization domain-like receptor containing protein 3 (NLRP3) could promote synovial inflammation. Previous studies have shown that electroacupuncture (EA) has potential anti-inflammatory effect. However, the effect of EA treatment on OA remains unclear. The aim of this study was to investigate the effect of applied EA on OA and joint pain and its relationship with NLRP3 inflammasome. The Hartley guinea pigs with naturally occurring OA at age 18 months were chosen as the OA model and treated with EA for 4 weeks. Mechanical allodynia was quantified by using von Frey filaments. The expression of NLRP3 inflammasome and the downstream proinflammatory factors in the cartilage tissue were quantified. Our results showed that EA treatment significantly reduces mechanical allodynia, improves the articular cartilage structure, and decreases the fibrillation on the cartilage surface in guinea pigs with spontaneous osteoarthritis. Moreover, we also found that EA treatment attenuates the NLRP3 inflammasome activation and suppresses the protein expression levels of caspase-1 and IL-1β in the cartilage tissue. Our findings suggest that EA treatment attenuates OA and joint pain by suppressing NLRP3 inflammasome activation and support further investigation of the potential therapeutic tactics.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>32952587</pmid><doi>10.1155/2020/5476064</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-7916-8823</orcidid><orcidid>https://orcid.org/0000-0003-4715-0973</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Acupuncture Arthritis Cartilage Cartilage (articular) Cartilage diseases Caspase-1 Degeneration Electroacupuncture Fibrillation Filaments IL-1β Inflammasomes Inflammation Joint diseases Oligomerization Osteoarthritis Pain Pain perception Protein expression Proteins Software Tumor necrosis factor-TNF |
| title | Electroacupuncture Alleviates Osteoarthritis by Suppressing NLRP3 Inflammasome Activation in Guinea Pigs |
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