A rapid and low-cost method for genomic DNA extraction from the cyanobacterium Synechocystis
Abstract A two-step method is reported for preparation of genomic DNA from the model cyanobacterium Synechocystis that can be performed with minimal equipment and reagents in about an hour. High yields of genetic material can be obtained (200–450 ng/μl) with reasonable purity. A further ethanol prec...
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| Veröffentlicht in: | Biology Methods & Protocols 2020-01, Vol.5 (1), p.bpaa011 |
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| creator | Harrison, Dale J A Thompson, Elinor P |
| description | Abstract
A two-step method is reported for preparation of genomic DNA from the model cyanobacterium Synechocystis that can be performed with minimal equipment and reagents in about an hour. High yields of genetic material can be obtained (200–450 ng/μl) with reasonable purity. A further ethanol precipitation step can be included but is not necessary if template is simply required for polymerase chain reaction (PCR) or digestion. This new protocol is helpful for amplification of genes of interest in early-stage research projects and for low throughput screening of transformants. It is more reliable than colony PCR of Synechocystis cultures, and less involved and cheaper than existing clean-DNA preparation methods. It represents an unusually simple and reliable extraction protocol for the growing body of research making use of this cyanobacterium. |
| doi_str_mv | 10.1093/biomethods/bpaa011 |
| format | Article |
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A two-step method is reported for preparation of genomic DNA from the model cyanobacterium Synechocystis that can be performed with minimal equipment and reagents in about an hour. High yields of genetic material can be obtained (200–450 ng/μl) with reasonable purity. A further ethanol precipitation step can be included but is not necessary if template is simply required for polymerase chain reaction (PCR) or digestion. This new protocol is helpful for amplification of genes of interest in early-stage research projects and for low throughput screening of transformants. It is more reliable than colony PCR of Synechocystis cultures, and less involved and cheaper than existing clean-DNA preparation methods. It represents an unusually simple and reliable extraction protocol for the growing body of research making use of this cyanobacterium.</description><identifier>ISSN: 2396-8923</identifier><identifier>EISSN: 2396-8923</identifier><identifier>DOI: 10.1093/biomethods/bpaa011</identifier><identifier>PMID: 32913895</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Analysis ; Chemical tests and reagents ; DNA ; Ethylenediaminetetraacetic acid ; Genes ; Genetic research ; Methods ; Methods Manuscript</subject><ispartof>Biology Methods & Protocols, 2020-01, Vol.5 (1), p.bpaa011</ispartof><rights>The Author(s) 2020. Published by Oxford University Press. 2020</rights><rights>The Author(s) 2020. Published by Oxford University Press.</rights><rights>COPYRIGHT 2020 Oxford University Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c541t-7635d75ebba291445a2a80f01693728e392fe6c7038175ab4e3094227f09e54f3</citedby><cites>FETCH-LOGICAL-c541t-7635d75ebba291445a2a80f01693728e392fe6c7038175ab4e3094227f09e54f3</cites><orcidid>0000-0002-6434-9290</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7474859/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7474859/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,1604,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32913895$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Harrison, Dale J A</creatorcontrib><creatorcontrib>Thompson, Elinor P</creatorcontrib><title>A rapid and low-cost method for genomic DNA extraction from the cyanobacterium Synechocystis</title><title>Biology Methods & Protocols</title><addtitle>Biol Methods Protoc</addtitle><description>Abstract
A two-step method is reported for preparation of genomic DNA from the model cyanobacterium Synechocystis that can be performed with minimal equipment and reagents in about an hour. High yields of genetic material can be obtained (200–450 ng/μl) with reasonable purity. A further ethanol precipitation step can be included but is not necessary if template is simply required for polymerase chain reaction (PCR) or digestion. This new protocol is helpful for amplification of genes of interest in early-stage research projects and for low throughput screening of transformants. It is more reliable than colony PCR of Synechocystis cultures, and less involved and cheaper than existing clean-DNA preparation methods. It represents an unusually simple and reliable extraction protocol for the growing body of research making use of this cyanobacterium.</description><subject>Analysis</subject><subject>Chemical tests and reagents</subject><subject>DNA</subject><subject>Ethylenediaminetetraacetic acid</subject><subject>Genes</subject><subject>Genetic research</subject><subject>Methods</subject><subject>Methods Manuscript</subject><issn>2396-8923</issn><issn>2396-8923</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>TOX</sourceid><recordid>eNqNUU1v1TAQtBAVrdr-AQ7IRy5p_ZnEF6SnAm2lCg7ADclynPV7Rokd7KTw_n1d5VHKDe3Bq_XMaHYHodeUXFCi-GXn4wjzLvb5spuMIZS-QCeMq7pqFeMvn_XH6DznH4QQ2gpJCX2FjjlTlLdKnqDvG5zM5HtsQo-H-KuyMc94VcYuJryFEEdv8ftPGwy_52Ts7GPALsURzzvAdm9C7MoUkl9G_GUfwO6i3efZ5zN05MyQ4fzwnqJvHz98vbqp7j5f315t7iorBZ2rpuaybyR0nSm-hJCGmZY4QmvFG9YCV8xBbRvCW9pI0wngRAnGGkcUSOH4KXq36k5LN0JvIRSfg56SH03a62i8_vcn-J3exnvdiEa0UhWBtweBFH8ukGc9-mxhGEyAuGTNhKA1ZYqRAr1YoVszgPbBxceblOqhnCkGcL7MN3WrlFCk4YXAVoJNMecE7skXJfoxSv03Sn2IspDePN_oifInuAKoVkBcpv8RfACR-K6H</recordid><startdate>20200101</startdate><enddate>20200101</enddate><creator>Harrison, Dale J A</creator><creator>Thompson, Elinor P</creator><general>Oxford University Press</general><scope>TOX</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IAO</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-6434-9290</orcidid></search><sort><creationdate>20200101</creationdate><title>A rapid and low-cost method for genomic DNA extraction from the cyanobacterium Synechocystis</title><author>Harrison, Dale J A ; Thompson, Elinor P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c541t-7635d75ebba291445a2a80f01693728e392fe6c7038175ab4e3094227f09e54f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Analysis</topic><topic>Chemical tests and reagents</topic><topic>DNA</topic><topic>Ethylenediaminetetraacetic acid</topic><topic>Genes</topic><topic>Genetic research</topic><topic>Methods</topic><topic>Methods Manuscript</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Harrison, Dale J A</creatorcontrib><creatorcontrib>Thompson, Elinor P</creatorcontrib><collection>Oxford Open</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale Academic OneFile Select</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biology Methods & Protocols</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Harrison, Dale J A</au><au>Thompson, Elinor P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A rapid and low-cost method for genomic DNA extraction from the cyanobacterium Synechocystis</atitle><jtitle>Biology Methods & Protocols</jtitle><addtitle>Biol Methods Protoc</addtitle><date>2020-01-01</date><risdate>2020</risdate><volume>5</volume><issue>1</issue><spage>bpaa011</spage><pages>bpaa011-</pages><issn>2396-8923</issn><eissn>2396-8923</eissn><abstract>Abstract
A two-step method is reported for preparation of genomic DNA from the model cyanobacterium Synechocystis that can be performed with minimal equipment and reagents in about an hour. High yields of genetic material can be obtained (200–450 ng/μl) with reasonable purity. A further ethanol precipitation step can be included but is not necessary if template is simply required for polymerase chain reaction (PCR) or digestion. This new protocol is helpful for amplification of genes of interest in early-stage research projects and for low throughput screening of transformants. It is more reliable than colony PCR of Synechocystis cultures, and less involved and cheaper than existing clean-DNA preparation methods. It represents an unusually simple and reliable extraction protocol for the growing body of research making use of this cyanobacterium.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>32913895</pmid><doi>10.1093/biomethods/bpaa011</doi><orcidid>https://orcid.org/0000-0002-6434-9290</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Directory of Open Access Journals; Oxford Open; PubMed Central; EZB Electronic Journals Library |
| subjects | Analysis Chemical tests and reagents DNA Ethylenediaminetetraacetic acid Genes Genetic research Methods Methods Manuscript |
| title | A rapid and low-cost method for genomic DNA extraction from the cyanobacterium Synechocystis |
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