Enhancement of target specificity of CRISPR–Cas12a by using a chimeric DNA–RNA guide

Abstract The CRISPR–Cas9 system is widely used for target-specific genome engineering. CRISPR–Cas12a (Cpf1) is one of the CRISPR effectors that controls target genes by recognizing thymine-rich protospacer adjacent motif (PAM) sequences. Cas12a has a higher sensitivity to mismatches in the guide RNA...

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Veröffentlicht in:	Nucleic acids research 2020-09, Vol.48 (15), p.8601-8616
Hauptverfasser:	Kim, Hanseop, Lee, Wi-jae, Oh, Yeounsun, Kang, Seung-Hun, Hur, Junho K, Lee, Hyomin, Song, WooJeung, Lim, Kyung-Seob, Park, Young-Ho, Song, Bong-Seok, Jin, Yeung Bae, Jun, Bong-Hyun, Jung, Cheulhee, Lee, Dong-Seok, Kim, Sun-Uk, Lee, Seung Hwan
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - genetics Base Pair Mismatch - genetics CRISPR-Associated Proteins - genetics CRISPR-Cas Systems - genetics DNA - genetics DNA Cleavage Endodeoxyribonucleases - genetics Gene Editing Humans Models, Molecular Mutation - genetics Nucleic Acid Conformation Nucleic Acid Enzymes RNA - genetics RNA, Circular - genetics RNA, Guide, CRISPR-Cas Systems - genetics
Online-Zugang:	Volltext
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