Immunochromatographic test and ELISA for the detection of glutamate dehydrogenase (GDH) and A/B toxins as an alternative for the diagnosis of Clostridioides (Clostridium) difficile–associated diarrhea in foals and neonatal piglets
Considering the lack of studies evaluating the performance of commercially available methods for diagnosis of Clostridioides ( Clostridium ) difficile infection (CDI) in animals, the present study aimed to assess an immunochromatographic test for detection of glutamate dehydrogenase (GDH) and A/B to...
Gespeichert in:
| Veröffentlicht in: | Brazilian journal of microbiology 2020-09, Vol.51 (3), p.1459-1462 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1462 |
|---|---|
| container_issue | 3 |
| container_start_page | 1459 |
| container_title | Brazilian journal of microbiology |
| container_volume | 51 |
| creator | Ramos, Carolina Pantuzza Lopes, Emily Oliveira Oliveira Júnior, Carlos Augusto Diniz, Amanda Nádia Lobato, Francisco Carlos Faria Silva, Rodrigo Otávio Silveira |
| description | Considering the lack of studies evaluating the performance of commercially available methods for diagnosis of
Clostridioides
(
Clostridium
)
difficile
infection (CDI) in animals, the present study aimed to assess an immunochromatographic test for detection of glutamate dehydrogenase (GDH) and A/B toxins of
C
.
difficile
, also evaluated by an ELISA kit, in foals and neonatal piglets. Intestinal contents of 47 piglets and feces of 35 foals were tested to GDH antigen and A/B toxins in a lateral flow method (Ecodiagnostica, Brazil). Also, these samples were submitted to A/B toxin detection by an ELISA kit (
C
.
difficile
Tox A/B II, Techlab Inc., USA), using the toxigenic culture (TC) as the reference method. The GDH component of the lateral flow test showed sensitivity and negative predictive value (NPV) of 100% and a high specificity in samples of piglets (82.61%) and foals (100%). Detection of A/B toxins using the lateral flow test and the ELISA resulted in a specificity of 100% in samples of both species. On the other hand, the sensibility ranged from 54.2 to 90% for the ELISA and from 12.5 to 60% for the lateral flow test for piglets’ and foals’ samples, respectively. In conclusion, the present work suggests that the lateral flow test for GDH detection could be a useful method for diagnosing CDI in these species. On the other hand, the low sensitivity of the lateral flow test for A/B toxins might compromise its utility in piglets. |
| doi_str_mv | 10.1007/s42770-020-00275-4 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7455628</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2438213719</sourcerecordid><originalsourceid>FETCH-LOGICAL-c474t-b3889affd312e1599419a327d399c1f650c5aba199477bdbc26b3ea125233acd3</originalsourceid><addsrcrecordid>eNp9Ustu1DAUjRCIlsIPsECW2LSL0PiRONkgDUNpRxqJBbC2bmwncZXYg-1UdMc_8Ies-Aw8M-20bJBs-XHPw_I9WfYaF-9wUfDzwAjnRV6QNAvCy5w9yY5xxeucsaJ8mvYl5nlNa3KUvQjhOoHKgpHn2REltKJlVR9nf1bTNFsnB-8miK73sBmMRFGHiMAqdLFefVmgznkUB42UjlpG4yxyHerHOUIiba-HW-Vdry0EjU4vP16d7ciL8w8ouh_GBgRpWARj1N5CNDf6QdNAb10wYau5HF2I3ijjjNIBnR7O83SWkF1npBn175-_IAQnTTJXWwHvBw3I2CQKY9h5W-2SEYxoY_pRx_Aye9almn51t55k3z5dfF1e5evPl6vlYp1LxlnMW1rXDXSdophoXDYNww1QwhVtGom7qixkCS3gVOC8Va0kVUs1YFISSkEqepK93-tu5nbSSmobPYxi480E_lY4MOLfijWD6N2N4KwsK1Ingbd3At59n1MfxLWb06eNQRCWeokpx01CkT1KeheC193BARdimw6xT4dI6RC7dAiWSG8ev-1AuY9DAtA9IKSS7bV_8P6P7F8LmsxU</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2438213719</pqid></control><display><type>article</type><title>Immunochromatographic test and ELISA for the detection of glutamate dehydrogenase (GDH) and A/B toxins as an alternative for the diagnosis of Clostridioides (Clostridium) difficile–associated diarrhea in foals and neonatal piglets</title><source>MEDLINE</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>SpringerLink Journals - AutoHoldings</source><creator>Ramos, Carolina Pantuzza ; Lopes, Emily Oliveira ; Oliveira Júnior, Carlos Augusto ; Diniz, Amanda Nádia ; Lobato, Francisco Carlos Faria ; Silva, Rodrigo Otávio Silveira</creator><creatorcontrib>Ramos, Carolina Pantuzza ; Lopes, Emily Oliveira ; Oliveira Júnior, Carlos Augusto ; Diniz, Amanda Nádia ; Lobato, Francisco Carlos Faria ; Silva, Rodrigo Otávio Silveira</creatorcontrib><description>Considering the lack of studies evaluating the performance of commercially available methods for diagnosis of
Clostridioides
(
Clostridium
)
difficile
infection (CDI) in animals, the present study aimed to assess an immunochromatographic test for detection of glutamate dehydrogenase (GDH) and A/B toxins of
C
.
difficile
, also evaluated by an ELISA kit, in foals and neonatal piglets. Intestinal contents of 47 piglets and feces of 35 foals were tested to GDH antigen and A/B toxins in a lateral flow method (Ecodiagnostica, Brazil). Also, these samples were submitted to A/B toxin detection by an ELISA kit (
C
.
difficile
Tox A/B II, Techlab Inc., USA), using the toxigenic culture (TC) as the reference method. The GDH component of the lateral flow test showed sensitivity and negative predictive value (NPV) of 100% and a high specificity in samples of piglets (82.61%) and foals (100%). Detection of A/B toxins using the lateral flow test and the ELISA resulted in a specificity of 100% in samples of both species. On the other hand, the sensibility ranged from 54.2 to 90% for the ELISA and from 12.5 to 60% for the lateral flow test for piglets’ and foals’ samples, respectively. In conclusion, the present work suggests that the lateral flow test for GDH detection could be a useful method for diagnosing CDI in these species. On the other hand, the low sensitivity of the lateral flow test for A/B toxins might compromise its utility in piglets.</description><identifier>ISSN: 1517-8382</identifier><identifier>EISSN: 1678-4405</identifier><identifier>DOI: 10.1007/s42770-020-00275-4</identifier><identifier>PMID: 32363568</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Animals ; Animals, Newborn - microbiology ; Antigens ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Bacterial Toxins - analysis ; Bacterial Toxins - metabolism ; Biomedical and Life Sciences ; Brazil ; Clostridioides difficile - enzymology ; Clostridioides difficile - isolation & purification ; Clostridioides difficile - metabolism ; Clostridium ; Clostridium Infections - diagnosis ; Clostridium Infections - microbiology ; Clostridium Infections - veterinary ; Dehydrogenase ; Dehydrogenases ; Diagnosis ; Diarrhea ; Diarrhea - diagnosis ; Diarrhea - microbiology ; Diarrhea - veterinary ; Enzyme-linked immunosorbent assay ; Enzyme-Linked Immunosorbent Assay - methods ; Feces - microbiology ; Food Microbiology ; Glutamate dehydrogenase ; Glutamate Dehydrogenase - analysis ; Glutamate Dehydrogenase - metabolism ; Horse Diseases - diagnosis ; Horse Diseases - microbiology ; Horses ; Immunoassay - methods ; Immunoassay - veterinary ; Intestine ; Juveniles ; Life Sciences ; Medical Microbiology ; Microbial Ecology ; Microbial Genetics and Genomics ; Microbiology ; Mycology ; Neonates ; Performance evaluation ; Sensitivity ; Sensitivity analysis ; Swine ; Swine Diseases - diagnosis ; Swine Diseases - microbiology ; Toxins ; Veterinary Microbiology - Short Communication</subject><ispartof>Brazilian journal of microbiology, 2020-09, Vol.51 (3), p.1459-1462</ispartof><rights>Sociedade Brasileira de Microbiologia 2020</rights><rights>Sociedade Brasileira de Microbiologia 2020.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-b3889affd312e1599419a327d399c1f650c5aba199477bdbc26b3ea125233acd3</citedby><cites>FETCH-LOGICAL-c474t-b3889affd312e1599419a327d399c1f650c5aba199477bdbc26b3ea125233acd3</cites><orcidid>0000-0002-9609-0746 ; 0000-0001-8205-8465 ; 0000-0002-5287-5779 ; 0000-0002-0656-1285 ; 0000-0003-0074-2488 ; 0000-0003-2792-058X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7455628/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7455628/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,41467,42536,51297,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32363568$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ramos, Carolina Pantuzza</creatorcontrib><creatorcontrib>Lopes, Emily Oliveira</creatorcontrib><creatorcontrib>Oliveira Júnior, Carlos Augusto</creatorcontrib><creatorcontrib>Diniz, Amanda Nádia</creatorcontrib><creatorcontrib>Lobato, Francisco Carlos Faria</creatorcontrib><creatorcontrib>Silva, Rodrigo Otávio Silveira</creatorcontrib><title>Immunochromatographic test and ELISA for the detection of glutamate dehydrogenase (GDH) and A/B toxins as an alternative for the diagnosis of Clostridioides (Clostridium) difficile–associated diarrhea in foals and neonatal piglets</title><title>Brazilian journal of microbiology</title><addtitle>Braz J Microbiol</addtitle><addtitle>Braz J Microbiol</addtitle><description>Considering the lack of studies evaluating the performance of commercially available methods for diagnosis of
Clostridioides
(
Clostridium
)
difficile
infection (CDI) in animals, the present study aimed to assess an immunochromatographic test for detection of glutamate dehydrogenase (GDH) and A/B toxins of
C
.
difficile
, also evaluated by an ELISA kit, in foals and neonatal piglets. Intestinal contents of 47 piglets and feces of 35 foals were tested to GDH antigen and A/B toxins in a lateral flow method (Ecodiagnostica, Brazil). Also, these samples were submitted to A/B toxin detection by an ELISA kit (
C
.
difficile
Tox A/B II, Techlab Inc., USA), using the toxigenic culture (TC) as the reference method. The GDH component of the lateral flow test showed sensitivity and negative predictive value (NPV) of 100% and a high specificity in samples of piglets (82.61%) and foals (100%). Detection of A/B toxins using the lateral flow test and the ELISA resulted in a specificity of 100% in samples of both species. On the other hand, the sensibility ranged from 54.2 to 90% for the ELISA and from 12.5 to 60% for the lateral flow test for piglets’ and foals’ samples, respectively. In conclusion, the present work suggests that the lateral flow test for GDH detection could be a useful method for diagnosing CDI in these species. On the other hand, the low sensitivity of the lateral flow test for A/B toxins might compromise its utility in piglets.</description><subject>Animals</subject><subject>Animals, Newborn - microbiology</subject><subject>Antigens</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacterial Toxins - analysis</subject><subject>Bacterial Toxins - metabolism</subject><subject>Biomedical and Life Sciences</subject><subject>Brazil</subject><subject>Clostridioides difficile - enzymology</subject><subject>Clostridioides difficile - isolation & purification</subject><subject>Clostridioides difficile - metabolism</subject><subject>Clostridium</subject><subject>Clostridium Infections - diagnosis</subject><subject>Clostridium Infections - microbiology</subject><subject>Clostridium Infections - veterinary</subject><subject>Dehydrogenase</subject><subject>Dehydrogenases</subject><subject>Diagnosis</subject><subject>Diarrhea</subject><subject>Diarrhea - diagnosis</subject><subject>Diarrhea - microbiology</subject><subject>Diarrhea - veterinary</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Feces - microbiology</subject><subject>Food Microbiology</subject><subject>Glutamate dehydrogenase</subject><subject>Glutamate Dehydrogenase - analysis</subject><subject>Glutamate Dehydrogenase - metabolism</subject><subject>Horse Diseases - diagnosis</subject><subject>Horse Diseases - microbiology</subject><subject>Horses</subject><subject>Immunoassay - methods</subject><subject>Immunoassay - veterinary</subject><subject>Intestine</subject><subject>Juveniles</subject><subject>Life Sciences</subject><subject>Medical Microbiology</subject><subject>Microbial Ecology</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Mycology</subject><subject>Neonates</subject><subject>Performance evaluation</subject><subject>Sensitivity</subject><subject>Sensitivity analysis</subject><subject>Swine</subject><subject>Swine Diseases - diagnosis</subject><subject>Swine Diseases - microbiology</subject><subject>Toxins</subject><subject>Veterinary Microbiology - Short Communication</subject><issn>1517-8382</issn><issn>1678-4405</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9Ustu1DAUjRCIlsIPsECW2LSL0PiRONkgDUNpRxqJBbC2bmwncZXYg-1UdMc_8Ies-Aw8M-20bJBs-XHPw_I9WfYaF-9wUfDzwAjnRV6QNAvCy5w9yY5xxeucsaJ8mvYl5nlNa3KUvQjhOoHKgpHn2REltKJlVR9nf1bTNFsnB-8miK73sBmMRFGHiMAqdLFefVmgznkUB42UjlpG4yxyHerHOUIiba-HW-Vdry0EjU4vP16d7ciL8w8ouh_GBgRpWARj1N5CNDf6QdNAb10wYau5HF2I3ijjjNIBnR7O83SWkF1npBn175-_IAQnTTJXWwHvBw3I2CQKY9h5W-2SEYxoY_pRx_Aye9almn51t55k3z5dfF1e5evPl6vlYp1LxlnMW1rXDXSdophoXDYNww1QwhVtGom7qixkCS3gVOC8Va0kVUs1YFISSkEqepK93-tu5nbSSmobPYxi480E_lY4MOLfijWD6N2N4KwsK1Ingbd3At59n1MfxLWb06eNQRCWeokpx01CkT1KeheC193BARdimw6xT4dI6RC7dAiWSG8ev-1AuY9DAtA9IKSS7bV_8P6P7F8LmsxU</recordid><startdate>20200901</startdate><enddate>20200901</enddate><creator>Ramos, Carolina Pantuzza</creator><creator>Lopes, Emily Oliveira</creator><creator>Oliveira Júnior, Carlos Augusto</creator><creator>Diniz, Amanda Nádia</creator><creator>Lobato, Francisco Carlos Faria</creator><creator>Silva, Rodrigo Otávio Silveira</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-9609-0746</orcidid><orcidid>https://orcid.org/0000-0001-8205-8465</orcidid><orcidid>https://orcid.org/0000-0002-5287-5779</orcidid><orcidid>https://orcid.org/0000-0002-0656-1285</orcidid><orcidid>https://orcid.org/0000-0003-0074-2488</orcidid><orcidid>https://orcid.org/0000-0003-2792-058X</orcidid></search><sort><creationdate>20200901</creationdate><title>Immunochromatographic test and ELISA for the detection of glutamate dehydrogenase (GDH) and A/B toxins as an alternative for the diagnosis of Clostridioides (Clostridium) difficile–associated diarrhea in foals and neonatal piglets</title><author>Ramos, Carolina Pantuzza ; Lopes, Emily Oliveira ; Oliveira Júnior, Carlos Augusto ; Diniz, Amanda Nádia ; Lobato, Francisco Carlos Faria ; Silva, Rodrigo Otávio Silveira</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-b3889affd312e1599419a327d399c1f650c5aba199477bdbc26b3ea125233acd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Animals, Newborn - microbiology</topic><topic>Antigens</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacterial Toxins - analysis</topic><topic>Bacterial Toxins - metabolism</topic><topic>Biomedical and Life Sciences</topic><topic>Brazil</topic><topic>Clostridioides difficile - enzymology</topic><topic>Clostridioides difficile - isolation & purification</topic><topic>Clostridioides difficile - metabolism</topic><topic>Clostridium</topic><topic>Clostridium Infections - diagnosis</topic><topic>Clostridium Infections - microbiology</topic><topic>Clostridium Infections - veterinary</topic><topic>Dehydrogenase</topic><topic>Dehydrogenases</topic><topic>Diagnosis</topic><topic>Diarrhea</topic><topic>Diarrhea - diagnosis</topic><topic>Diarrhea - microbiology</topic><topic>Diarrhea - veterinary</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Feces - microbiology</topic><topic>Food Microbiology</topic><topic>Glutamate dehydrogenase</topic><topic>Glutamate Dehydrogenase - analysis</topic><topic>Glutamate Dehydrogenase - metabolism</topic><topic>Horse Diseases - diagnosis</topic><topic>Horse Diseases - microbiology</topic><topic>Horses</topic><topic>Immunoassay - methods</topic><topic>Immunoassay - veterinary</topic><topic>Intestine</topic><topic>Juveniles</topic><topic>Life Sciences</topic><topic>Medical Microbiology</topic><topic>Microbial Ecology</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbiology</topic><topic>Mycology</topic><topic>Neonates</topic><topic>Performance evaluation</topic><topic>Sensitivity</topic><topic>Sensitivity analysis</topic><topic>Swine</topic><topic>Swine Diseases - diagnosis</topic><topic>Swine Diseases - microbiology</topic><topic>Toxins</topic><topic>Veterinary Microbiology - Short Communication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ramos, Carolina Pantuzza</creatorcontrib><creatorcontrib>Lopes, Emily Oliveira</creatorcontrib><creatorcontrib>Oliveira Júnior, Carlos Augusto</creatorcontrib><creatorcontrib>Diniz, Amanda Nádia</creatorcontrib><creatorcontrib>Lobato, Francisco Carlos Faria</creatorcontrib><creatorcontrib>Silva, Rodrigo Otávio Silveira</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Brazilian journal of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ramos, Carolina Pantuzza</au><au>Lopes, Emily Oliveira</au><au>Oliveira Júnior, Carlos Augusto</au><au>Diniz, Amanda Nádia</au><au>Lobato, Francisco Carlos Faria</au><au>Silva, Rodrigo Otávio Silveira</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunochromatographic test and ELISA for the detection of glutamate dehydrogenase (GDH) and A/B toxins as an alternative for the diagnosis of Clostridioides (Clostridium) difficile–associated diarrhea in foals and neonatal piglets</atitle><jtitle>Brazilian journal of microbiology</jtitle><stitle>Braz J Microbiol</stitle><addtitle>Braz J Microbiol</addtitle><date>2020-09-01</date><risdate>2020</risdate><volume>51</volume><issue>3</issue><spage>1459</spage><epage>1462</epage><pages>1459-1462</pages><issn>1517-8382</issn><eissn>1678-4405</eissn><abstract>Considering the lack of studies evaluating the performance of commercially available methods for diagnosis of
Clostridioides
(
Clostridium
)
difficile
infection (CDI) in animals, the present study aimed to assess an immunochromatographic test for detection of glutamate dehydrogenase (GDH) and A/B toxins of
C
.
difficile
, also evaluated by an ELISA kit, in foals and neonatal piglets. Intestinal contents of 47 piglets and feces of 35 foals were tested to GDH antigen and A/B toxins in a lateral flow method (Ecodiagnostica, Brazil). Also, these samples were submitted to A/B toxin detection by an ELISA kit (
C
.
difficile
Tox A/B II, Techlab Inc., USA), using the toxigenic culture (TC) as the reference method. The GDH component of the lateral flow test showed sensitivity and negative predictive value (NPV) of 100% and a high specificity in samples of piglets (82.61%) and foals (100%). Detection of A/B toxins using the lateral flow test and the ELISA resulted in a specificity of 100% in samples of both species. On the other hand, the sensibility ranged from 54.2 to 90% for the ELISA and from 12.5 to 60% for the lateral flow test for piglets’ and foals’ samples, respectively. In conclusion, the present work suggests that the lateral flow test for GDH detection could be a useful method for diagnosing CDI in these species. On the other hand, the low sensitivity of the lateral flow test for A/B toxins might compromise its utility in piglets.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>32363568</pmid><doi>10.1007/s42770-020-00275-4</doi><tpages>4</tpages><orcidid>https://orcid.org/0000-0002-9609-0746</orcidid><orcidid>https://orcid.org/0000-0001-8205-8465</orcidid><orcidid>https://orcid.org/0000-0002-5287-5779</orcidid><orcidid>https://orcid.org/0000-0002-0656-1285</orcidid><orcidid>https://orcid.org/0000-0003-0074-2488</orcidid><orcidid>https://orcid.org/0000-0003-2792-058X</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1517-8382 |
| ispartof | Brazilian journal of microbiology, 2020-09, Vol.51 (3), p.1459-1462 |
| issn | 1517-8382 1678-4405 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7455628 |
| source | MEDLINE; PubMed Central; Alma/SFX Local Collection; SpringerLink Journals - AutoHoldings |
| subjects | Animals Animals, Newborn - microbiology Antigens Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacterial Toxins - analysis Bacterial Toxins - metabolism Biomedical and Life Sciences Brazil Clostridioides difficile - enzymology Clostridioides difficile - isolation & purification Clostridioides difficile - metabolism Clostridium Clostridium Infections - diagnosis Clostridium Infections - microbiology Clostridium Infections - veterinary Dehydrogenase Dehydrogenases Diagnosis Diarrhea Diarrhea - diagnosis Diarrhea - microbiology Diarrhea - veterinary Enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Feces - microbiology Food Microbiology Glutamate dehydrogenase Glutamate Dehydrogenase - analysis Glutamate Dehydrogenase - metabolism Horse Diseases - diagnosis Horse Diseases - microbiology Horses Immunoassay - methods Immunoassay - veterinary Intestine Juveniles Life Sciences Medical Microbiology Microbial Ecology Microbial Genetics and Genomics Microbiology Mycology Neonates Performance evaluation Sensitivity Sensitivity analysis Swine Swine Diseases - diagnosis Swine Diseases - microbiology Toxins Veterinary Microbiology - Short Communication |
| title | Immunochromatographic test and ELISA for the detection of glutamate dehydrogenase (GDH) and A/B toxins as an alternative for the diagnosis of Clostridioides (Clostridium) difficile–associated diarrhea in foals and neonatal piglets |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-24T07%3A43%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Immunochromatographic%20test%20and%20ELISA%20for%20the%20detection%20of%20glutamate%20dehydrogenase%20(GDH)%20and%20A/B%20toxins%20as%20an%20alternative%20for%20the%20diagnosis%20of%20Clostridioides%20(Clostridium)%20difficile%E2%80%93associated%20diarrhea%20in%20foals%20and%20neonatal%20piglets&rft.jtitle=Brazilian%20journal%20of%20microbiology&rft.au=Ramos,%20Carolina%20Pantuzza&rft.date=2020-09-01&rft.volume=51&rft.issue=3&rft.spage=1459&rft.epage=1462&rft.pages=1459-1462&rft.issn=1517-8382&rft.eissn=1678-4405&rft_id=info:doi/10.1007/s42770-020-00275-4&rft_dat=%3Cproquest_pubme%3E2438213719%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2438213719&rft_id=info:pmid/32363568&rfr_iscdi=true |