Influence of PCR cycle number on 16S rRNA gene amplicon sequencing of low biomass samples

The objective of this study was to evaluate the effects of increased PCR cycle number on sequencing results from samples with low microbial biomass, including bovine milk, and murine pelage and blood. We hypothesized that subjecting DNA from such samples to higher PCR cycle numbers would increase 16...

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Veröffentlicht in:	Journal of microbiological methods 2020-09, Vol.176, p.106033-106033, Article 106033
Hauptverfasser:	Witzke, Monica C., Gullic, Alexis, Yang, Peggy, Bivens, Nathan J., Adkins, Pamela R.F., Ericsson, Aaron C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Blood Dairy cattle Illumina sequencing Low biomass Microbiome Milk Mouse Pelage
Online-Zugang:	Volltext
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