Metagenome-wide association study of the alterations in the intestinal microbiome composition of ankylosing spondylitis patients and the effect of traditional and herbal treatment
. Ankylosing spondylitis (AS) is a systemic progressive disease with an unknown etiology that may be related to the gut microbiome. Therefore, a more thorough understanding of its pathogenesis is necessary for directing future therapy. . We aimed to determine the differences in intestinal microbial...
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| creator | Huang, RunYue Li, Fang Zhou, Yingyan Zeng, Zhenhua He, Xiaohong Fang, Lihua Pan, Feng Chen, Yile Lin, Jiehua Li, Jie Qiu, Dongni Tian, Yinping Tan, Xi Song, Yanni Xu, Yongyue Lai, Yonghui Yi, Hao Gao, Qiang Fang, Xiaodong Shi, Mingming Zhou, Chu Huang, Jinqun He, Yi-Ting |
| description | . Ankylosing spondylitis (AS) is a systemic progressive disease with an unknown etiology that may be related to the gut microbiome. Therefore, a more thorough understanding of its pathogenesis is necessary for directing future therapy.
. We aimed to determine the differences in intestinal microbial composition between healthy individuals and patients with AS who received and who did not receive treatment interventions. In parallel, the pathology of AS in each patient was analysed to better understand the link between AS treatment and the intestinal microbiota of the patients.
. Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis. All samples were sequenced using Illumina HiSeq 4000 and the microbial composition was determined.
. Four species were enriched in the patients with AS:
,
,
and
(HC vs. NM, |
| doi_str_mv | 10.1099/jmm.0.001107 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7451032</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2319490710</sourcerecordid><originalsourceid>FETCH-LOGICAL-c384t-5c1e0a0f970de5dc3d41eea0f8a4a98a197d34efed79b26a48eeea1cb9b9ba173</originalsourceid><addsrcrecordid>eNpVkUFv1DAQhS0EotuFG2eUIwey2LGzji9IqIKCVMSlPVuOPdl1Sexge0H7u_iDnWZLReVD7HnvfRPNEPKG0Q2jSn24naYN3VDKGJXPyIoJyet2K8RzsqK0aepmy9ozcp7zLXok5-olOeNMyg7TK_L3OxSzgxAnqP94B5XJOVpvio-hyuXgjlUcqrJHYSyQlnqufFhKPhTIxQczVpO3KfYeMZWN0xyzXwiYNeHnccR32FV5jsEdR5RyNSMKQsmouwUGwwC2LN2ScUscuffqHlKP15LAlAkzr8iLwYwZXj981-Tmy-fri6_11Y_LbxefrmrLO1Hq1jKghg5KUgets9wJBoCFzgijOsOUdFzAAE6qvtka0QHKzPYKj8FRrcnHE3c-9BM4i62TGfWc_GTSUUfj9VMl-L3exd9aipZR3iDg3QMgxV8HHJWefLYwjiZAPGTdcKaEohLNa_L-ZMUx5pxgeGzDqL7fs8Y9a6pPe0b72_9_7dH8b7H8Do5krFM</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2319490710</pqid></control><display><type>article</type><title>Metagenome-wide association study of the alterations in the intestinal microbiome composition of ankylosing spondylitis patients and the effect of traditional and herbal treatment</title><source>MEDLINE</source><source>Microbiology Society</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Huang, RunYue ; Li, Fang ; Zhou, Yingyan ; Zeng, Zhenhua ; He, Xiaohong ; Fang, Lihua ; Pan, Feng ; Chen, Yile ; Lin, Jiehua ; Li, Jie ; Qiu, Dongni ; Tian, Yinping ; Tan, Xi ; Song, Yanni ; Xu, Yongyue ; Lai, Yonghui ; Yi, Hao ; Gao, Qiang ; Fang, Xiaodong ; Shi, Mingming ; Zhou, Chu ; Huang, Jinqun ; He, Yi-Ting</creator><creatorcontrib>Huang, RunYue ; Li, Fang ; Zhou, Yingyan ; Zeng, Zhenhua ; He, Xiaohong ; Fang, Lihua ; Pan, Feng ; Chen, Yile ; Lin, Jiehua ; Li, Jie ; Qiu, Dongni ; Tian, Yinping ; Tan, Xi ; Song, Yanni ; Xu, Yongyue ; Lai, Yonghui ; Yi, Hao ; Gao, Qiang ; Fang, Xiaodong ; Shi, Mingming ; Zhou, Chu ; Huang, Jinqun ; He, Yi-Ting</creatorcontrib><description>. Ankylosing spondylitis (AS) is a systemic progressive disease with an unknown etiology that may be related to the gut microbiome. Therefore, a more thorough understanding of its pathogenesis is necessary for directing future therapy.
. We aimed to determine the differences in intestinal microbial composition between healthy individuals and patients with AS who received and who did not receive treatment interventions. In parallel, the pathology of AS in each patient was analysed to better understand the link between AS treatment and the intestinal microbiota of the patients.
. Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis. All samples were sequenced using Illumina HiSeq 4000 and the microbial composition was determined.
. Four species were enriched in the patients with AS:
,
,
and
(HC vs. NM,
<0.05); only
was found to be significantly changed in the NM-HC comparison. No additional species were found in the HC vs. CHM analysis, which indicated a beneficial effect of CHM in removing the other three strains.
was found to be significantly increased in the comparison between the HC and WM groups, along with four other species (
, Clostridiales bacterium 1_7_47FAA,
and
). The patients with AS harboured more bacterial species associated with carbohydrate metabolism and glycan biosynthesis in their faeces. They also had bacterial profiles less able to biodegrade xenobiotics or synthesize and transport vitamins.
. The gut microbiota of the patients with AS varied from that of the HCs, and the treatment had an impact on this divergence. Our data provide insight that could guide improvements in AS treatment.</description><identifier>ISSN: 0022-2615</identifier><identifier>EISSN: 1473-5644</identifier><identifier>DOI: 10.1099/jmm.0.001107</identifier><identifier>PMID: 31778109</identifier><language>eng</language><publisher>England: Microbiology Society</publisher><subject>Adolescent ; Adult ; Drugs, Chinese Herbal - therapeutic use ; Dysbiosis ; Gastrointestinal Microbiome ; Humans ; Metagenome ; Middle Aged ; Spondylitis, Ankylosing - drug therapy ; Spondylitis, Ankylosing - metabolism ; Spondylitis, Ankylosing - microbiology ; Young Adult</subject><ispartof>Journal of medical microbiology, 2020-06, Vol.69 (6), p.797-805</ispartof><rights>2020 The Authors 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-5c1e0a0f970de5dc3d41eea0f8a4a98a197d34efed79b26a48eeea1cb9b9ba173</citedby><cites>FETCH-LOGICAL-c384t-5c1e0a0f970de5dc3d41eea0f8a4a98a197d34efed79b26a48eeea1cb9b9ba173</cites><orcidid>0000-0002-0521-9154</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,3744,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31778109$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, RunYue</creatorcontrib><creatorcontrib>Li, Fang</creatorcontrib><creatorcontrib>Zhou, Yingyan</creatorcontrib><creatorcontrib>Zeng, Zhenhua</creatorcontrib><creatorcontrib>He, Xiaohong</creatorcontrib><creatorcontrib>Fang, Lihua</creatorcontrib><creatorcontrib>Pan, Feng</creatorcontrib><creatorcontrib>Chen, Yile</creatorcontrib><creatorcontrib>Lin, Jiehua</creatorcontrib><creatorcontrib>Li, Jie</creatorcontrib><creatorcontrib>Qiu, Dongni</creatorcontrib><creatorcontrib>Tian, Yinping</creatorcontrib><creatorcontrib>Tan, Xi</creatorcontrib><creatorcontrib>Song, Yanni</creatorcontrib><creatorcontrib>Xu, Yongyue</creatorcontrib><creatorcontrib>Lai, Yonghui</creatorcontrib><creatorcontrib>Yi, Hao</creatorcontrib><creatorcontrib>Gao, Qiang</creatorcontrib><creatorcontrib>Fang, Xiaodong</creatorcontrib><creatorcontrib>Shi, Mingming</creatorcontrib><creatorcontrib>Zhou, Chu</creatorcontrib><creatorcontrib>Huang, Jinqun</creatorcontrib><creatorcontrib>He, Yi-Ting</creatorcontrib><title>Metagenome-wide association study of the alterations in the intestinal microbiome composition of ankylosing spondylitis patients and the effect of traditional and herbal treatment</title><title>Journal of medical microbiology</title><addtitle>J Med Microbiol</addtitle><description>. Ankylosing spondylitis (AS) is a systemic progressive disease with an unknown etiology that may be related to the gut microbiome. Therefore, a more thorough understanding of its pathogenesis is necessary for directing future therapy.
. We aimed to determine the differences in intestinal microbial composition between healthy individuals and patients with AS who received and who did not receive treatment interventions. In parallel, the pathology of AS in each patient was analysed to better understand the link between AS treatment and the intestinal microbiota of the patients.
. Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis. All samples were sequenced using Illumina HiSeq 4000 and the microbial composition was determined.
. Four species were enriched in the patients with AS:
,
,
and
(HC vs. NM,
<0.05); only
was found to be significantly changed in the NM-HC comparison. No additional species were found in the HC vs. CHM analysis, which indicated a beneficial effect of CHM in removing the other three strains.
was found to be significantly increased in the comparison between the HC and WM groups, along with four other species (
, Clostridiales bacterium 1_7_47FAA,
and
). The patients with AS harboured more bacterial species associated with carbohydrate metabolism and glycan biosynthesis in their faeces. They also had bacterial profiles less able to biodegrade xenobiotics or synthesize and transport vitamins.
. The gut microbiota of the patients with AS varied from that of the HCs, and the treatment had an impact on this divergence. Our data provide insight that could guide improvements in AS treatment.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Drugs, Chinese Herbal - therapeutic use</subject><subject>Dysbiosis</subject><subject>Gastrointestinal Microbiome</subject><subject>Humans</subject><subject>Metagenome</subject><subject>Middle Aged</subject><subject>Spondylitis, Ankylosing - drug therapy</subject><subject>Spondylitis, Ankylosing - metabolism</subject><subject>Spondylitis, Ankylosing - microbiology</subject><subject>Young Adult</subject><issn>0022-2615</issn><issn>1473-5644</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkUFv1DAQhS0EotuFG2eUIwey2LGzji9IqIKCVMSlPVuOPdl1Sexge0H7u_iDnWZLReVD7HnvfRPNEPKG0Q2jSn24naYN3VDKGJXPyIoJyet2K8RzsqK0aepmy9ozcp7zLXok5-olOeNMyg7TK_L3OxSzgxAnqP94B5XJOVpvio-hyuXgjlUcqrJHYSyQlnqufFhKPhTIxQczVpO3KfYeMZWN0xyzXwiYNeHnccR32FV5jsEdR5RyNSMKQsmouwUGwwC2LN2ScUscuffqHlKP15LAlAkzr8iLwYwZXj981-Tmy-fri6_11Y_LbxefrmrLO1Hq1jKghg5KUgets9wJBoCFzgijOsOUdFzAAE6qvtka0QHKzPYKj8FRrcnHE3c-9BM4i62TGfWc_GTSUUfj9VMl-L3exd9aipZR3iDg3QMgxV8HHJWefLYwjiZAPGTdcKaEohLNa_L-ZMUx5pxgeGzDqL7fs8Y9a6pPe0b72_9_7dH8b7H8Do5krFM</recordid><startdate>202006</startdate><enddate>202006</enddate><creator>Huang, RunYue</creator><creator>Li, Fang</creator><creator>Zhou, Yingyan</creator><creator>Zeng, Zhenhua</creator><creator>He, Xiaohong</creator><creator>Fang, Lihua</creator><creator>Pan, Feng</creator><creator>Chen, Yile</creator><creator>Lin, Jiehua</creator><creator>Li, Jie</creator><creator>Qiu, Dongni</creator><creator>Tian, Yinping</creator><creator>Tan, Xi</creator><creator>Song, Yanni</creator><creator>Xu, Yongyue</creator><creator>Lai, Yonghui</creator><creator>Yi, Hao</creator><creator>Gao, Qiang</creator><creator>Fang, Xiaodong</creator><creator>Shi, Mingming</creator><creator>Zhou, Chu</creator><creator>Huang, Jinqun</creator><creator>He, Yi-Ting</creator><general>Microbiology Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-0521-9154</orcidid></search><sort><creationdate>202006</creationdate><title>Metagenome-wide association study of the alterations in the intestinal microbiome composition of ankylosing spondylitis patients and the effect of traditional and herbal treatment</title><author>Huang, RunYue ; Li, Fang ; Zhou, Yingyan ; Zeng, Zhenhua ; He, Xiaohong ; Fang, Lihua ; Pan, Feng ; Chen, Yile ; Lin, Jiehua ; Li, Jie ; Qiu, Dongni ; Tian, Yinping ; Tan, Xi ; Song, Yanni ; Xu, Yongyue ; Lai, Yonghui ; Yi, Hao ; Gao, Qiang ; Fang, Xiaodong ; Shi, Mingming ; Zhou, Chu ; Huang, Jinqun ; He, Yi-Ting</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-5c1e0a0f970de5dc3d41eea0f8a4a98a197d34efed79b26a48eeea1cb9b9ba173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Drugs, Chinese Herbal - therapeutic use</topic><topic>Dysbiosis</topic><topic>Gastrointestinal Microbiome</topic><topic>Humans</topic><topic>Metagenome</topic><topic>Middle Aged</topic><topic>Spondylitis, Ankylosing - drug therapy</topic><topic>Spondylitis, Ankylosing - metabolism</topic><topic>Spondylitis, Ankylosing - microbiology</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, RunYue</creatorcontrib><creatorcontrib>Li, Fang</creatorcontrib><creatorcontrib>Zhou, Yingyan</creatorcontrib><creatorcontrib>Zeng, Zhenhua</creatorcontrib><creatorcontrib>He, Xiaohong</creatorcontrib><creatorcontrib>Fang, Lihua</creatorcontrib><creatorcontrib>Pan, Feng</creatorcontrib><creatorcontrib>Chen, Yile</creatorcontrib><creatorcontrib>Lin, Jiehua</creatorcontrib><creatorcontrib>Li, Jie</creatorcontrib><creatorcontrib>Qiu, Dongni</creatorcontrib><creatorcontrib>Tian, Yinping</creatorcontrib><creatorcontrib>Tan, Xi</creatorcontrib><creatorcontrib>Song, Yanni</creatorcontrib><creatorcontrib>Xu, Yongyue</creatorcontrib><creatorcontrib>Lai, Yonghui</creatorcontrib><creatorcontrib>Yi, Hao</creatorcontrib><creatorcontrib>Gao, Qiang</creatorcontrib><creatorcontrib>Fang, Xiaodong</creatorcontrib><creatorcontrib>Shi, Mingming</creatorcontrib><creatorcontrib>Zhou, Chu</creatorcontrib><creatorcontrib>Huang, Jinqun</creatorcontrib><creatorcontrib>He, Yi-Ting</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of medical microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, RunYue</au><au>Li, Fang</au><au>Zhou, Yingyan</au><au>Zeng, Zhenhua</au><au>He, Xiaohong</au><au>Fang, Lihua</au><au>Pan, Feng</au><au>Chen, Yile</au><au>Lin, Jiehua</au><au>Li, Jie</au><au>Qiu, Dongni</au><au>Tian, Yinping</au><au>Tan, Xi</au><au>Song, Yanni</au><au>Xu, Yongyue</au><au>Lai, Yonghui</au><au>Yi, Hao</au><au>Gao, Qiang</au><au>Fang, Xiaodong</au><au>Shi, Mingming</au><au>Zhou, Chu</au><au>Huang, Jinqun</au><au>He, Yi-Ting</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Metagenome-wide association study of the alterations in the intestinal microbiome composition of ankylosing spondylitis patients and the effect of traditional and herbal treatment</atitle><jtitle>Journal of medical microbiology</jtitle><addtitle>J Med Microbiol</addtitle><date>2020-06</date><risdate>2020</risdate><volume>69</volume><issue>6</issue><spage>797</spage><epage>805</epage><pages>797-805</pages><issn>0022-2615</issn><eissn>1473-5644</eissn><abstract>. Ankylosing spondylitis (AS) is a systemic progressive disease with an unknown etiology that may be related to the gut microbiome. Therefore, a more thorough understanding of its pathogenesis is necessary for directing future therapy.
. We aimed to determine the differences in intestinal microbial composition between healthy individuals and patients with AS who received and who did not receive treatment interventions. In parallel, the pathology of AS in each patient was analysed to better understand the link between AS treatment and the intestinal microbiota of the patients.
. Sixty-six faecal DNA samples, including 37 from healthy controls (HCs), 11 from patients with untreated AS (NM), 7 from patients treated with nonsteroidal anti-inflammatory drugs (e.g. celecoxib; WM) and 11 from patients treated with Chinese herbal medicine (CHM), such as the Bushen-Qiangdu-Zhilv decoction, were collected and used in the drug effect analysis. All samples were sequenced using Illumina HiSeq 4000 and the microbial composition was determined.
. Four species were enriched in the patients with AS:
,
,
and
(HC vs. NM,
<0.05); only
was found to be significantly changed in the NM-HC comparison. No additional species were found in the HC vs. CHM analysis, which indicated a beneficial effect of CHM in removing the other three strains.
was found to be significantly increased in the comparison between the HC and WM groups, along with four other species (
, Clostridiales bacterium 1_7_47FAA,
and
). The patients with AS harboured more bacterial species associated with carbohydrate metabolism and glycan biosynthesis in their faeces. They also had bacterial profiles less able to biodegrade xenobiotics or synthesize and transport vitamins.
. The gut microbiota of the patients with AS varied from that of the HCs, and the treatment had an impact on this divergence. Our data provide insight that could guide improvements in AS treatment.</abstract><cop>England</cop><pub>Microbiology Society</pub><pmid>31778109</pmid><doi>10.1099/jmm.0.001107</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-0521-9154</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Adolescent Adult Drugs, Chinese Herbal - therapeutic use Dysbiosis Gastrointestinal Microbiome Humans Metagenome Middle Aged Spondylitis, Ankylosing - drug therapy Spondylitis, Ankylosing - metabolism Spondylitis, Ankylosing - microbiology Young Adult |
| title | Metagenome-wide association study of the alterations in the intestinal microbiome composition of ankylosing spondylitis patients and the effect of traditional and herbal treatment |
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