Cord blood serum harvesting by hydroxyethyl starch: a fetal bovine serum alternative in expansion of umbilical cord-derived mesenchymal stem cells
As a widely used cell culture supplement, fetal bovine serum (FBS) harbor high content of growth, proliferation, and adhesion factors. However, high cost, bio-safety, possible xenogeneic agent transmission, finite accessible, and ethical controversy are major obstacles that discourage the use of thi...
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| Veröffentlicht in: | Cytotechnology (Dordrecht) 2020-08, Vol.72 (4), p.551-567 |
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| creator | Samareh Salavati Pour, Maryam Vahidi, Reza Lashkari, Mahla Derakhshani, Ali Ameri, Zahra Farsinejad, Alireza |
| description | As a widely used cell culture supplement, fetal bovine serum (FBS) harbor high content of growth, proliferation, and adhesion factors. However, high cost, bio-safety, possible xenogeneic agent transmission, finite accessible, and ethical controversy are major obstacles that discourage the use of this additive. Accordingly, novel alternatives have been proposed with various pros and cons. Still, caution should be taken in choosing suitable substitute given that the alteration in the main aspects of cultured cells can be biased the consequences of clinical applications. Herein, the authors evaluated the impact of cord blood serum harvesting by hydroxyethyl starch (CBS-HES), as an enriched source of growth factors, on the basic mesenchymal stem cells (MSCs) characteristics. In the present experiment, umbilical cord-derived MSCs were isolated and continuously nourished with Dulbecco’s Modified Eagle Medium containing either 10, 15, and 20% CBS-HES or FBSs to compare their morphology, immunophenotype, growth and proliferation rate, death rate, cell cycle, and gene expression profiles. Although all enriched media supported the expansion of MSCs with comparable morphology, cell surface markers, death rate, c-MYC and p16 expression, and growth rate, CBS-HES treated cells significantly (P |
| doi_str_mv | 10.1007/s10616-020-00404-9 |
| format | Article |
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However, high cost, bio-safety, possible xenogeneic agent transmission, finite accessible, and ethical controversy are major obstacles that discourage the use of this additive. Accordingly, novel alternatives have been proposed with various pros and cons. Still, caution should be taken in choosing suitable substitute given that the alteration in the main aspects of cultured cells can be biased the consequences of clinical applications. Herein, the authors evaluated the impact of cord blood serum harvesting by hydroxyethyl starch (CBS-HES), as an enriched source of growth factors, on the basic mesenchymal stem cells (MSCs) characteristics. In the present experiment, umbilical cord-derived MSCs were isolated and continuously nourished with Dulbecco’s Modified Eagle Medium containing either 10, 15, and 20% CBS-HES or FBSs to compare their morphology, immunophenotype, growth and proliferation rate, death rate, cell cycle, and gene expression profiles. Although all enriched media supported the expansion of MSCs with comparable morphology, cell surface markers, death rate, c-MYC and p16 expression, and growth rate, CBS-HES treated cells significantly (P < 0.05) expressed more hTERT gene in a concentration-dependent manner. Yet no significant shift was observed in the cell cycle of cultured cells using the same concentrations of additives, a finding which further confirmed by Ki-67 immunostaining. CBS-HES as an available and affordable additive, seems to be an optimal, relatively safe, and promising FBS alternative for cultivation, propagation, and subsequent clinical applications of MSCs.</description><identifier>ISSN: 0920-9069</identifier><identifier>EISSN: 1573-0778</identifier><identifier>DOI: 10.1007/s10616-020-00404-9</identifier><identifier>PMID: 32601794</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Adaptation ; Biochemistry ; Biomedicine ; Biotechnology ; c-Myc protein ; Cell culture ; Cell cycle ; Cell death ; Cell surface ; Chemistry ; Chemistry and Materials Science ; Cord blood ; Cytokines ; Cytology ; Enrichment media ; Experiments ; Fetal calf serum ; Gene expression ; Growth factors ; Growth rate ; Hydroxyethyl starch ; Medical research ; Mesenchymal stem cells ; Morphology ; Myc protein ; Original ; Original Article ; Penicillin ; Propagation ; Stem cells ; Surface markers ; Telomerase reverse transcriptase ; Therapeutic applications ; Umbilical cord</subject><ispartof>Cytotechnology (Dordrecht), 2020-08, Vol.72 (4), p.551-567</ispartof><rights>Springer Nature B.V. 2020</rights><rights>Springer Nature B.V. 2020.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c516t-21851414da46a2027bc247bd33da2bcf1a523d492dc61cfe8c83271c856552973</citedby><cites>FETCH-LOGICAL-c516t-21851414da46a2027bc247bd33da2bcf1a523d492dc61cfe8c83271c856552973</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7450036/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2918256692?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,21388,27924,27925,33744,33745,41488,42557,43805,51319,53791,53793,64385,64387,64389,72469</link.rule.ids></links><search><creatorcontrib>Samareh Salavati Pour, Maryam</creatorcontrib><creatorcontrib>Vahidi, Reza</creatorcontrib><creatorcontrib>Lashkari, Mahla</creatorcontrib><creatorcontrib>Derakhshani, Ali</creatorcontrib><creatorcontrib>Ameri, Zahra</creatorcontrib><creatorcontrib>Farsinejad, Alireza</creatorcontrib><title>Cord blood serum harvesting by hydroxyethyl starch: a fetal bovine serum alternative in expansion of umbilical cord-derived mesenchymal stem cells</title><title>Cytotechnology (Dordrecht)</title><addtitle>Cytotechnology</addtitle><description>As a widely used cell culture supplement, fetal bovine serum (FBS) harbor high content of growth, proliferation, and adhesion factors. However, high cost, bio-safety, possible xenogeneic agent transmission, finite accessible, and ethical controversy are major obstacles that discourage the use of this additive. Accordingly, novel alternatives have been proposed with various pros and cons. Still, caution should be taken in choosing suitable substitute given that the alteration in the main aspects of cultured cells can be biased the consequences of clinical applications. Herein, the authors evaluated the impact of cord blood serum harvesting by hydroxyethyl starch (CBS-HES), as an enriched source of growth factors, on the basic mesenchymal stem cells (MSCs) characteristics. In the present experiment, umbilical cord-derived MSCs were isolated and continuously nourished with Dulbecco’s Modified Eagle Medium containing either 10, 15, and 20% CBS-HES or FBSs to compare their morphology, immunophenotype, growth and proliferation rate, death rate, cell cycle, and gene expression profiles. Although all enriched media supported the expansion of MSCs with comparable morphology, cell surface markers, death rate, c-MYC and p16 expression, and growth rate, CBS-HES treated cells significantly (P < 0.05) expressed more hTERT gene in a concentration-dependent manner. Yet no significant shift was observed in the cell cycle of cultured cells using the same concentrations of additives, a finding which further confirmed by Ki-67 immunostaining. CBS-HES as an available and affordable additive, seems to be an optimal, relatively safe, and promising FBS alternative for cultivation, propagation, and subsequent clinical applications of MSCs.</description><subject>Adaptation</subject><subject>Biochemistry</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>c-Myc protein</subject><subject>Cell culture</subject><subject>Cell cycle</subject><subject>Cell death</subject><subject>Cell surface</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Cord blood</subject><subject>Cytokines</subject><subject>Cytology</subject><subject>Enrichment media</subject><subject>Experiments</subject><subject>Fetal calf serum</subject><subject>Gene expression</subject><subject>Growth factors</subject><subject>Growth rate</subject><subject>Hydroxyethyl starch</subject><subject>Medical research</subject><subject>Mesenchymal stem cells</subject><subject>Morphology</subject><subject>Myc protein</subject><subject>Original</subject><subject>Original Article</subject><subject>Penicillin</subject><subject>Propagation</subject><subject>Stem cells</subject><subject>Surface markers</subject><subject>Telomerase reverse transcriptase</subject><subject>Therapeutic applications</subject><subject>Umbilical cord</subject><issn>0920-9069</issn><issn>1573-0778</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9ks1u1DAURiMEokPhBVhZYsMmcP0TO2aBhEYtIFVi064tx3YmrhJ7sJNR8xo8cT3MCASLrrzw-Y59r76qeovhAwYQHzMGjnkNBGoABqyWz6oNbgStQYj2ebUBWa4kcHlRvcr5HgCkwPRldUEJBywk21S_tjFZ1I0xWpRdWiY06HRwefZhh7oVDatN8WF187COKM86meET0qh3sx5RFw8-uHNOj7NLQc_-4JAPyD3sdcg-BhR7tEydH70pEVOeq61LhbJoctkFM6yTPrrdhIwbx_y6etHrMbs35_Oyuru-ut1-q29-fP2-_XJTmwbzuSa4bTDDzGrGNQEiOkOY6CylVpPO9Fg3hFomiTUcm961pqVEYNM2vGmIFPSy-nzy7pducta4MCc9qn3yk06ritqrf2-CH9QuHpRgDQDlRfD-LEjx51J2piafjyPo4OKSFWFYgmw5oQV99x96H5eyrbFQErek4VySJylGBSNl8KOLnCiTYs7J9X--jEEdi6FOxVClGOp3MZQsIXoK5QKHnUt_1U-kHgEUErwd</recordid><startdate>20200801</startdate><enddate>20200801</enddate><creator>Samareh Salavati Pour, Maryam</creator><creator>Vahidi, Reza</creator><creator>Lashkari, Mahla</creator><creator>Derakhshani, Ali</creator><creator>Ameri, Zahra</creator><creator>Farsinejad, Alireza</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20200801</creationdate><title>Cord blood serum harvesting by hydroxyethyl starch: a fetal bovine serum alternative in expansion of umbilical cord-derived mesenchymal stem cells</title><author>Samareh Salavati Pour, Maryam ; Vahidi, Reza ; Lashkari, Mahla ; Derakhshani, Ali ; Ameri, Zahra ; Farsinejad, Alireza</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c516t-21851414da46a2027bc247bd33da2bcf1a523d492dc61cfe8c83271c856552973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adaptation</topic><topic>Biochemistry</topic><topic>Biomedicine</topic><topic>Biotechnology</topic><topic>c-Myc protein</topic><topic>Cell culture</topic><topic>Cell cycle</topic><topic>Cell death</topic><topic>Cell surface</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Cord blood</topic><topic>Cytokines</topic><topic>Cytology</topic><topic>Enrichment media</topic><topic>Experiments</topic><topic>Fetal calf serum</topic><topic>Gene expression</topic><topic>Growth factors</topic><topic>Growth rate</topic><topic>Hydroxyethyl starch</topic><topic>Medical research</topic><topic>Mesenchymal stem cells</topic><topic>Morphology</topic><topic>Myc protein</topic><topic>Original</topic><topic>Original Article</topic><topic>Penicillin</topic><topic>Propagation</topic><topic>Stem cells</topic><topic>Surface markers</topic><topic>Telomerase reverse transcriptase</topic><topic>Therapeutic applications</topic><topic>Umbilical cord</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Samareh Salavati Pour, Maryam</creatorcontrib><creatorcontrib>Vahidi, Reza</creatorcontrib><creatorcontrib>Lashkari, Mahla</creatorcontrib><creatorcontrib>Derakhshani, Ali</creatorcontrib><creatorcontrib>Ameri, Zahra</creatorcontrib><creatorcontrib>Farsinejad, Alireza</creatorcontrib><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cytotechnology (Dordrecht)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Samareh Salavati Pour, Maryam</au><au>Vahidi, Reza</au><au>Lashkari, Mahla</au><au>Derakhshani, Ali</au><au>Ameri, Zahra</au><au>Farsinejad, Alireza</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cord blood serum harvesting by hydroxyethyl starch: a fetal bovine serum alternative in expansion of umbilical cord-derived mesenchymal stem cells</atitle><jtitle>Cytotechnology (Dordrecht)</jtitle><stitle>Cytotechnology</stitle><date>2020-08-01</date><risdate>2020</risdate><volume>72</volume><issue>4</issue><spage>551</spage><epage>567</epage><pages>551-567</pages><issn>0920-9069</issn><eissn>1573-0778</eissn><abstract>As a widely used cell culture supplement, fetal bovine serum (FBS) harbor high content of growth, proliferation, and adhesion factors. However, high cost, bio-safety, possible xenogeneic agent transmission, finite accessible, and ethical controversy are major obstacles that discourage the use of this additive. Accordingly, novel alternatives have been proposed with various pros and cons. Still, caution should be taken in choosing suitable substitute given that the alteration in the main aspects of cultured cells can be biased the consequences of clinical applications. Herein, the authors evaluated the impact of cord blood serum harvesting by hydroxyethyl starch (CBS-HES), as an enriched source of growth factors, on the basic mesenchymal stem cells (MSCs) characteristics. In the present experiment, umbilical cord-derived MSCs were isolated and continuously nourished with Dulbecco’s Modified Eagle Medium containing either 10, 15, and 20% CBS-HES or FBSs to compare their morphology, immunophenotype, growth and proliferation rate, death rate, cell cycle, and gene expression profiles. Although all enriched media supported the expansion of MSCs with comparable morphology, cell surface markers, death rate, c-MYC and p16 expression, and growth rate, CBS-HES treated cells significantly (P < 0.05) expressed more hTERT gene in a concentration-dependent manner. Yet no significant shift was observed in the cell cycle of cultured cells using the same concentrations of additives, a finding which further confirmed by Ki-67 immunostaining. CBS-HES as an available and affordable additive, seems to be an optimal, relatively safe, and promising FBS alternative for cultivation, propagation, and subsequent clinical applications of MSCs.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>32601794</pmid><doi>10.1007/s10616-020-00404-9</doi><tpages>17</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adaptation Biochemistry Biomedicine Biotechnology c-Myc protein Cell culture Cell cycle Cell death Cell surface Chemistry Chemistry and Materials Science Cord blood Cytokines Cytology Enrichment media Experiments Fetal calf serum Gene expression Growth factors Growth rate Hydroxyethyl starch Medical research Mesenchymal stem cells Morphology Myc protein Original Original Article Penicillin Propagation Stem cells Surface markers Telomerase reverse transcriptase Therapeutic applications Umbilical cord |
| title | Cord blood serum harvesting by hydroxyethyl starch: a fetal bovine serum alternative in expansion of umbilical cord-derived mesenchymal stem cells |
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