A positive, growth-based PAM screen identifies noncanonical motifs recognized by the S. pyogenes Cas9

CRISPR technologies have overwhelmingly relied on the Cas9 (SpyCas9), with its consensus NGG and less preferred NAG and NGA protospacer-adjacent motifs (PAMs). Here, we report that SpyCas9 also recognizes sequences within an N(A/C/T)GG motif. These sequences were identified on the basis of preferent...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Science advances 2020-07, Vol.6 (29), p.eabb4054-eabb4054
Hauptverfasser:	Collias, D, Leenay, R T, Slotkowski, R A, Zuo, Z, Collins, S P, McGirr, B A, Liu, J, Beisel, C L
Format:	Artikel
Sprache:	eng
Schlagworte:	Life Sciences SciAdv r-articles Synthetic Biology
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!