A retrospective case control study identifies peripheral blood mononuclear cell albumin RNA expression as a biomarker for non-alcoholic fatty liver disease
Purpose Non-alcoholic fatty liver disease (NAFLD) improves after bariatric surgery. The aim of this study was to determine whether peripheral blood mononuclear cell albumin gene expression was related to NAFLD and whether albumin (ALB) and alpha fetoprotein (AFP) expression could be detected in whol...
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| Veröffentlicht in: | Langenbeck's archives of surgery 2020-03, Vol.405 (2), p.165-172 |
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| creator | Chu, Xin Karasinski, Kelsey Donellan, Sean Kaniper, Scott Wood, G. Craig Shi, Weixing Edwards, Michael A. Soans, Rohit Still, Christopher D. Gerhard, Glenn S. |
| description | Purpose
Non-alcoholic fatty liver disease (NAFLD) improves after bariatric surgery. The aim of this study was to determine whether peripheral blood mononuclear cell albumin gene expression was related to NAFLD and whether albumin (ALB) and alpha fetoprotein (AFP) expression could be detected in whole blood and visceral adipose tissue.
Methods
Using a retrospective case control study design, RNA isolated from peripheral blood mononuclear cells from patients prior to undergoing bariatric surgery was used for pooled microarray analysis. Quantitative polymerase chain reaction (QPCR) was used to analyze whole blood and visceral adipose tissue. Liver histology was obtained via intra-operative biopsy and clinical data extracted from the electronic health record.
Results
The albumin (ALB) gene was the second most up-regulated found in microarray analysis of peripheral blood mononuclear cell RNA from patients with hepatic lobular inflammation versus normal liver histology. Transcript levels of ALB were significantly different across those with normal (
n
= 50), steatosis (
n
= 50), lobular inflammation (
n
= 50), and peri-sinusoidal fibrosis (
n
= 50) liver histologies, with lobular inflammation 3.9 times higher than those with normal histology (
p
|
| doi_str_mv | 10.1007/s00423-019-01848-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7435277</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2325298599</sourcerecordid><originalsourceid>FETCH-LOGICAL-c446t-c11df8cff0362a174f87094010d9f13dbd30ce7ec44dcda0d5ea1d2865b6681f3</originalsourceid><addsrcrecordid>eNp9UcluFDEUbCEQWeAHOKB35NLBW28XpFEUFikCKYKz5bafMw5uu7G7I-Zb-FmcTBiRSw5e5FevXK-qqt5QckYJ6d5nQgTjNaFDWb3oa_KsOqaCNzUTDX1-uAt-VJ3kfEMIabtBvKyOOO1Z3xB-XP3ZQMIlxTyjXtwtgla5bDGUNw95Wc0OnMGwOOsww4zJzVtMysPoYzQwxRDDqj2qBBq9B-XHdXIBrr5uAH_PCXN2MYDKoGB0cVLpJyawMUFprJXXcRu902DVsuzAFwkJjMtYZLyqXljlM75-OE-rHx8vvp9_ri-_ffpyvrmstRDtUmtKje21tYS3TNFO2L4jgyCUmMFSbkbDicYOC9poo4hpUFHD-rYZ27anlp9WH_a88zpOaHSZtgwo5-SK2p2MysnHleC28jreyq74y7quELx7IEjx14p5kZPLd26ogHHNknHWsKFvhqFA2R6qi-c5oT18Q4m8S1XuU5UlVXmfqiSl6e3_Ag8t_2IsAL4H5FIK15jkTVxTKKY9RfsXPiGy-A</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2325298599</pqid></control><display><type>article</type><title>A retrospective case control study identifies peripheral blood mononuclear cell albumin RNA expression as a biomarker for non-alcoholic fatty liver disease</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Chu, Xin ; Karasinski, Kelsey ; Donellan, Sean ; Kaniper, Scott ; Wood, G. Craig ; Shi, Weixing ; Edwards, Michael A. ; Soans, Rohit ; Still, Christopher D. ; Gerhard, Glenn S.</creator><creatorcontrib>Chu, Xin ; Karasinski, Kelsey ; Donellan, Sean ; Kaniper, Scott ; Wood, G. Craig ; Shi, Weixing ; Edwards, Michael A. ; Soans, Rohit ; Still, Christopher D. ; Gerhard, Glenn S.</creatorcontrib><description>Purpose
Non-alcoholic fatty liver disease (NAFLD) improves after bariatric surgery. The aim of this study was to determine whether peripheral blood mononuclear cell albumin gene expression was related to NAFLD and whether albumin (ALB) and alpha fetoprotein (AFP) expression could be detected in whole blood and visceral adipose tissue.
Methods
Using a retrospective case control study design, RNA isolated from peripheral blood mononuclear cells from patients prior to undergoing bariatric surgery was used for pooled microarray analysis. Quantitative polymerase chain reaction (QPCR) was used to analyze whole blood and visceral adipose tissue. Liver histology was obtained via intra-operative biopsy and clinical data extracted from the electronic health record.
Results
The albumin (ALB) gene was the second most up-regulated found in microarray analysis of peripheral blood mononuclear cell RNA from patients with hepatic lobular inflammation versus normal liver histology. Transcript levels of ALB were significantly different across those with normal (
n
= 50), steatosis (
n
= 50), lobular inflammation (
n
= 50), and peri-sinusoidal fibrosis (
n
= 50) liver histologies, with lobular inflammation 3.9 times higher than those with normal histology (
p
< 0.017). Albumin expression levels decreased in 11/13 patients in paired samples obtained prior to and at 1 year after Roux-en-Y gastric bypass surgery. ALB expression could be detected in 23 visceral adipose tissue samples obtained intra-operatively and in 18/19 available paired whole blood samples. No significant correlation was found between ALB expression in visceral adipose tissue and whole blood RNA samples. Alpha fetoprotein expression as a marker of early hepatocytic differentiation was detected in 17/17 available VAT RNA samples, but in only 2/17 whole blood RNA samples.
Conclusion
Albumin RNA expression from blood cells may serve as a biomarker of NAFLD. Albumin and alpha fetoprotein appear to be ubiquitously expressed in visceral adipose tissue in patients with extreme obesity.</description><identifier>ISSN: 1435-2443</identifier><identifier>EISSN: 1435-2451</identifier><identifier>DOI: 10.1007/s00423-019-01848-0</identifier><identifier>PMID: 31828503</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Abdominal Surgery ; Adult ; Aged ; Albumins - genetics ; Albumins - metabolism ; alpha-Fetoproteins - metabolism ; Bariatric Surgery ; Biomarkers - metabolism ; Cardiac Surgery ; Case-Control Studies ; Female ; General Surgery ; Humans ; Intra-Abdominal Fat - metabolism ; Leukocytes, Mononuclear - metabolism ; Male ; Medicine ; Medicine & Public Health ; Middle Aged ; Non-alcoholic Fatty Liver Disease - diagnosis ; Non-alcoholic Fatty Liver Disease - metabolism ; Obesity, Morbid - complications ; Obesity, Morbid - metabolism ; Obesity, Morbid - surgery ; Original Article ; Real-Time Polymerase Chain Reaction ; RNA - metabolism ; Thoracic Surgery ; Tissue Array Analysis ; Traumatic Surgery ; Vascular Surgery</subject><ispartof>Langenbeck's archives of surgery, 2020-03, Vol.405 (2), p.165-172</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c446t-c11df8cff0362a174f87094010d9f13dbd30ce7ec44dcda0d5ea1d2865b6681f3</citedby><cites>FETCH-LOGICAL-c446t-c11df8cff0362a174f87094010d9f13dbd30ce7ec44dcda0d5ea1d2865b6681f3</cites><orcidid>0000-0001-6276-0337</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00423-019-01848-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00423-019-01848-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,780,784,885,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31828503$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chu, Xin</creatorcontrib><creatorcontrib>Karasinski, Kelsey</creatorcontrib><creatorcontrib>Donellan, Sean</creatorcontrib><creatorcontrib>Kaniper, Scott</creatorcontrib><creatorcontrib>Wood, G. Craig</creatorcontrib><creatorcontrib>Shi, Weixing</creatorcontrib><creatorcontrib>Edwards, Michael A.</creatorcontrib><creatorcontrib>Soans, Rohit</creatorcontrib><creatorcontrib>Still, Christopher D.</creatorcontrib><creatorcontrib>Gerhard, Glenn S.</creatorcontrib><title>A retrospective case control study identifies peripheral blood mononuclear cell albumin RNA expression as a biomarker for non-alcoholic fatty liver disease</title><title>Langenbeck's archives of surgery</title><addtitle>Langenbecks Arch Surg</addtitle><addtitle>Langenbecks Arch Surg</addtitle><description>Purpose
Non-alcoholic fatty liver disease (NAFLD) improves after bariatric surgery. The aim of this study was to determine whether peripheral blood mononuclear cell albumin gene expression was related to NAFLD and whether albumin (ALB) and alpha fetoprotein (AFP) expression could be detected in whole blood and visceral adipose tissue.
Methods
Using a retrospective case control study design, RNA isolated from peripheral blood mononuclear cells from patients prior to undergoing bariatric surgery was used for pooled microarray analysis. Quantitative polymerase chain reaction (QPCR) was used to analyze whole blood and visceral adipose tissue. Liver histology was obtained via intra-operative biopsy and clinical data extracted from the electronic health record.
Results
The albumin (ALB) gene was the second most up-regulated found in microarray analysis of peripheral blood mononuclear cell RNA from patients with hepatic lobular inflammation versus normal liver histology. Transcript levels of ALB were significantly different across those with normal (
n
= 50), steatosis (
n
= 50), lobular inflammation (
n
= 50), and peri-sinusoidal fibrosis (
n
= 50) liver histologies, with lobular inflammation 3.9 times higher than those with normal histology (
p
< 0.017). Albumin expression levels decreased in 11/13 patients in paired samples obtained prior to and at 1 year after Roux-en-Y gastric bypass surgery. ALB expression could be detected in 23 visceral adipose tissue samples obtained intra-operatively and in 18/19 available paired whole blood samples. No significant correlation was found between ALB expression in visceral adipose tissue and whole blood RNA samples. Alpha fetoprotein expression as a marker of early hepatocytic differentiation was detected in 17/17 available VAT RNA samples, but in only 2/17 whole blood RNA samples.
Conclusion
Albumin RNA expression from blood cells may serve as a biomarker of NAFLD. Albumin and alpha fetoprotein appear to be ubiquitously expressed in visceral adipose tissue in patients with extreme obesity.</description><subject>Abdominal Surgery</subject><subject>Adult</subject><subject>Aged</subject><subject>Albumins - genetics</subject><subject>Albumins - metabolism</subject><subject>alpha-Fetoproteins - metabolism</subject><subject>Bariatric Surgery</subject><subject>Biomarkers - metabolism</subject><subject>Cardiac Surgery</subject><subject>Case-Control Studies</subject><subject>Female</subject><subject>General Surgery</subject><subject>Humans</subject><subject>Intra-Abdominal Fat - metabolism</subject><subject>Leukocytes, Mononuclear - metabolism</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Middle Aged</subject><subject>Non-alcoholic Fatty Liver Disease - diagnosis</subject><subject>Non-alcoholic Fatty Liver Disease - metabolism</subject><subject>Obesity, Morbid - complications</subject><subject>Obesity, Morbid - metabolism</subject><subject>Obesity, Morbid - surgery</subject><subject>Original Article</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>RNA - metabolism</subject><subject>Thoracic Surgery</subject><subject>Tissue Array Analysis</subject><subject>Traumatic Surgery</subject><subject>Vascular Surgery</subject><issn>1435-2443</issn><issn>1435-2451</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UcluFDEUbCEQWeAHOKB35NLBW28XpFEUFikCKYKz5bafMw5uu7G7I-Zb-FmcTBiRSw5e5FevXK-qqt5QckYJ6d5nQgTjNaFDWb3oa_KsOqaCNzUTDX1-uAt-VJ3kfEMIabtBvKyOOO1Z3xB-XP3ZQMIlxTyjXtwtgla5bDGUNw95Wc0OnMGwOOsww4zJzVtMysPoYzQwxRDDqj2qBBq9B-XHdXIBrr5uAH_PCXN2MYDKoGB0cVLpJyawMUFprJXXcRu902DVsuzAFwkJjMtYZLyqXljlM75-OE-rHx8vvp9_ri-_ffpyvrmstRDtUmtKje21tYS3TNFO2L4jgyCUmMFSbkbDicYOC9poo4hpUFHD-rYZ27anlp9WH_a88zpOaHSZtgwo5-SK2p2MysnHleC28jreyq74y7quELx7IEjx14p5kZPLd26ogHHNknHWsKFvhqFA2R6qi-c5oT18Q4m8S1XuU5UlVXmfqiSl6e3_Ag8t_2IsAL4H5FIK15jkTVxTKKY9RfsXPiGy-A</recordid><startdate>20200301</startdate><enddate>20200301</enddate><creator>Chu, Xin</creator><creator>Karasinski, Kelsey</creator><creator>Donellan, Sean</creator><creator>Kaniper, Scott</creator><creator>Wood, G. Craig</creator><creator>Shi, Weixing</creator><creator>Edwards, Michael A.</creator><creator>Soans, Rohit</creator><creator>Still, Christopher D.</creator><creator>Gerhard, Glenn S.</creator><general>Springer Berlin Heidelberg</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-6276-0337</orcidid></search><sort><creationdate>20200301</creationdate><title>A retrospective case control study identifies peripheral blood mononuclear cell albumin RNA expression as a biomarker for non-alcoholic fatty liver disease</title><author>Chu, Xin ; Karasinski, Kelsey ; Donellan, Sean ; Kaniper, Scott ; Wood, G. Craig ; Shi, Weixing ; Edwards, Michael A. ; Soans, Rohit ; Still, Christopher D. ; Gerhard, Glenn S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c446t-c11df8cff0362a174f87094010d9f13dbd30ce7ec44dcda0d5ea1d2865b6681f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Abdominal Surgery</topic><topic>Adult</topic><topic>Aged</topic><topic>Albumins - genetics</topic><topic>Albumins - metabolism</topic><topic>alpha-Fetoproteins - metabolism</topic><topic>Bariatric Surgery</topic><topic>Biomarkers - metabolism</topic><topic>Cardiac Surgery</topic><topic>Case-Control Studies</topic><topic>Female</topic><topic>General Surgery</topic><topic>Humans</topic><topic>Intra-Abdominal Fat - metabolism</topic><topic>Leukocytes, Mononuclear - metabolism</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Middle Aged</topic><topic>Non-alcoholic Fatty Liver Disease - diagnosis</topic><topic>Non-alcoholic Fatty Liver Disease - metabolism</topic><topic>Obesity, Morbid - complications</topic><topic>Obesity, Morbid - metabolism</topic><topic>Obesity, Morbid - surgery</topic><topic>Original Article</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>RNA - metabolism</topic><topic>Thoracic Surgery</topic><topic>Tissue Array Analysis</topic><topic>Traumatic Surgery</topic><topic>Vascular Surgery</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chu, Xin</creatorcontrib><creatorcontrib>Karasinski, Kelsey</creatorcontrib><creatorcontrib>Donellan, Sean</creatorcontrib><creatorcontrib>Kaniper, Scott</creatorcontrib><creatorcontrib>Wood, G. Craig</creatorcontrib><creatorcontrib>Shi, Weixing</creatorcontrib><creatorcontrib>Edwards, Michael A.</creatorcontrib><creatorcontrib>Soans, Rohit</creatorcontrib><creatorcontrib>Still, Christopher D.</creatorcontrib><creatorcontrib>Gerhard, Glenn S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Langenbeck's archives of surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chu, Xin</au><au>Karasinski, Kelsey</au><au>Donellan, Sean</au><au>Kaniper, Scott</au><au>Wood, G. Craig</au><au>Shi, Weixing</au><au>Edwards, Michael A.</au><au>Soans, Rohit</au><au>Still, Christopher D.</au><au>Gerhard, Glenn S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A retrospective case control study identifies peripheral blood mononuclear cell albumin RNA expression as a biomarker for non-alcoholic fatty liver disease</atitle><jtitle>Langenbeck's archives of surgery</jtitle><stitle>Langenbecks Arch Surg</stitle><addtitle>Langenbecks Arch Surg</addtitle><date>2020-03-01</date><risdate>2020</risdate><volume>405</volume><issue>2</issue><spage>165</spage><epage>172</epage><pages>165-172</pages><issn>1435-2443</issn><eissn>1435-2451</eissn><abstract>Purpose
Non-alcoholic fatty liver disease (NAFLD) improves after bariatric surgery. The aim of this study was to determine whether peripheral blood mononuclear cell albumin gene expression was related to NAFLD and whether albumin (ALB) and alpha fetoprotein (AFP) expression could be detected in whole blood and visceral adipose tissue.
Methods
Using a retrospective case control study design, RNA isolated from peripheral blood mononuclear cells from patients prior to undergoing bariatric surgery was used for pooled microarray analysis. Quantitative polymerase chain reaction (QPCR) was used to analyze whole blood and visceral adipose tissue. Liver histology was obtained via intra-operative biopsy and clinical data extracted from the electronic health record.
Results
The albumin (ALB) gene was the second most up-regulated found in microarray analysis of peripheral blood mononuclear cell RNA from patients with hepatic lobular inflammation versus normal liver histology. Transcript levels of ALB were significantly different across those with normal (
n
= 50), steatosis (
n
= 50), lobular inflammation (
n
= 50), and peri-sinusoidal fibrosis (
n
= 50) liver histologies, with lobular inflammation 3.9 times higher than those with normal histology (
p
< 0.017). Albumin expression levels decreased in 11/13 patients in paired samples obtained prior to and at 1 year after Roux-en-Y gastric bypass surgery. ALB expression could be detected in 23 visceral adipose tissue samples obtained intra-operatively and in 18/19 available paired whole blood samples. No significant correlation was found between ALB expression in visceral adipose tissue and whole blood RNA samples. Alpha fetoprotein expression as a marker of early hepatocytic differentiation was detected in 17/17 available VAT RNA samples, but in only 2/17 whole blood RNA samples.
Conclusion
Albumin RNA expression from blood cells may serve as a biomarker of NAFLD. Albumin and alpha fetoprotein appear to be ubiquitously expressed in visceral adipose tissue in patients with extreme obesity.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>31828503</pmid><doi>10.1007/s00423-019-01848-0</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-6276-0337</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Langenbeck's archives of surgery, 2020-03, Vol.405 (2), p.165-172 |
| issn | 1435-2443 1435-2451 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Abdominal Surgery Adult Aged Albumins - genetics Albumins - metabolism alpha-Fetoproteins - metabolism Bariatric Surgery Biomarkers - metabolism Cardiac Surgery Case-Control Studies Female General Surgery Humans Intra-Abdominal Fat - metabolism Leukocytes, Mononuclear - metabolism Male Medicine Medicine & Public Health Middle Aged Non-alcoholic Fatty Liver Disease - diagnosis Non-alcoholic Fatty Liver Disease - metabolism Obesity, Morbid - complications Obesity, Morbid - metabolism Obesity, Morbid - surgery Original Article Real-Time Polymerase Chain Reaction RNA - metabolism Thoracic Surgery Tissue Array Analysis Traumatic Surgery Vascular Surgery |
| title | A retrospective case control study identifies peripheral blood mononuclear cell albumin RNA expression as a biomarker for non-alcoholic fatty liver disease |
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