Donor hearts in the Sydney Heart Bank: reliable control but is it ‘normal’ heart?
Human heart samples from the Sydney Heart Bank have become a de facto standard against which others can be measured. Crucially, the heart bank contains a lot of donor heart material: for most researchers this is the hardest to obtain and yet is necessary since we can only study the pathological huma...
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| Veröffentlicht in: | Biophysical reviews 2020-08, Vol.12 (4), p.799-803 |
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| creator | Marston, Steven Jacques, Adam Bayliss, Christopher Dyer, Emma Memo, Massimiliano Papadaki, Maria Messer, Andrew |
| description | Human heart samples from the Sydney Heart Bank have become a de facto standard against which others can be measured. Crucially, the heart bank contains a lot of donor heart material: for most researchers this is the hardest to obtain and yet is necessary since we can only study the pathological human heart in comparison with a control, preferably a normal heart sample. It is not generally realised how important the control is for human heart studies. We review our studies on donor heart samples. We report the results obtained with 17 different donor samples collected from 1994 to 2011 and measured from 2005 to 2015 by our standard methodology for in vitro motility and troponin I phosphorylation measurements. The donor heart sample parameters are consistent between the hearts, over time and with different operators indicating that Sydney Heart Bank donor hearts are a valid baseline control for comparison with pathological heart samples. We also discuss to what extent donor heart samples are representative of the normal heart. |
| doi_str_mv | 10.1007/s12551-020-00740-2 |
| format | Article |
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| ispartof | Biophysical reviews, 2020-08, Vol.12 (4), p.799-803 |
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| source | EZB-FREE-00999 freely available EZB journals; PubMed Central; SpringerLink Journals - AutoHoldings |
| subjects | Biochemistry Biological and Medical Physics Biological Techniques Biomedical and Life Sciences Biophysics Calcium-binding protein Cell Biology Heart In vitro methods and tests Life Sciences Membrane Biology Nanotechnology Phosphorylation Review Troponin Troponin I |
| title | Donor hearts in the Sydney Heart Bank: reliable control but is it ‘normal’ heart? |
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